Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_J06
(584 letters)
Database: fruitfly
53,049 sequences; 24,988,368 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY089523-1|AAL90261.1| 143|Drosophila melanogaster GM14585p pro... 121 8e-28
AE013599-1845|AAF58277.2| 143|Drosophila melanogaster CG8415-PA... 121 8e-28
M19494-1|AAA28935.1| 140|Drosophila melanogaster protein ( D.me... 46 4e-05
BT015958-1|AAV36843.1| 140|Drosophila melanogaster RH56553p pro... 46 4e-05
AL133505-2|CAB65841.1| 140|Drosophila melanogaster EG:BACH59J11... 46 4e-05
AL133505-1|CAB63528.1| 154|Drosophila melanogaster EG:BACH59J11... 46 4e-05
AE014298-399|AAF45781.1| 140|Drosophila melanogaster CG7925-PB ... 46 4e-05
AJ250320-1|CAB65721.1| 140|Drosophila melanogaster technical kn... 43 3e-04
AY058686-1|AAL13915.1| 547|Drosophila melanogaster LD40728p pro... 31 1.5
AE014134-1900|AAF52961.2| 547|Drosophila melanogaster CG6187-PA... 31 1.5
BT016082-1|AAV36967.1| 568|Drosophila melanogaster LD47465p pro... 29 4.6
AY070520-1|AAL47991.1| 507|Drosophila melanogaster GH23534p pro... 29 4.6
AE014297-2300|AAX52959.1| 568|Drosophila melanogaster CG5201-PC... 29 4.6
AE014297-2299|AAF55383.1| 568|Drosophila melanogaster CG5201-PB... 29 4.6
AE014297-2298|AAN13728.1| 568|Drosophila melanogaster CG5201-PA... 29 4.6
AB004232-1|BAA22841.1| 568|Drosophila melanogaster DAD polypept... 29 4.6
AE014297-650|AAF54148.1| 358|Drosophila melanogaster CG18268-PA... 28 8.1
>AY089523-1|AAL90261.1| 143|Drosophila melanogaster GM14585p
protein.
Length = 143
Score = 121 bits (291), Expect = 8e-28
Identities = 55/61 (90%), Positives = 59/61 (96%)
Frame = +2
Query: 125 KNSTKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAF 304
K+ KAH+GT+WKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK+TAF
Sbjct: 25 KDYKKAHLGTRWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAF 84
Query: 305 V 307
V
Sbjct: 85 V 85
Score = 113 bits (271), Expect = 2e-25
Identities = 67/142 (47%), Positives = 77/142 (54%), Gaps = 1/142 (0%)
Frame = +3
Query: 60 PQAIRTARKHVNHRS*TAMGGQRIQRKPTWVR-NGRLTLSVVHLTQRAXXXXXXXXXXXX 236
P+ +RTARKHVNHR + ++ R H +
Sbjct: 4 PRGLRTARKHVNHRRDQRWADKDYKKAHLGTRWKANPFGGASHA--KGIVLEKVGVEAKQ 61
Query: 237 PTLPSANASVYSSLRTERK*PHSSPXDGCLNHIEXNDEXLVAGFGRKGHAVGDIPGVRFK 416
P ++ +K P DG LN+IE NDE LVAGFGRKGHAVGDIPGVRFK
Sbjct: 62 PNSAIRKCVRVQLIKNGKKITAFVPRDGSLNYIEENDEVLVAGFGRKGHAVGDIPGVRFK 121
Query: 417 VVKVANVSLLALYKEKKERPXS 482
VVKVANVSLLALYKEKKERP S
Sbjct: 122 VVKVANVSLLALYKEKKERPRS 143
Score = 30.7 bits (66), Expect = 1.5
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = +1
Query: 49 LGKPRRQYERRVST*TTAREQRWADKEFNESPHGYEMEG*PFRWCISRKG 198
+GKPR R R+QRWADK++ ++ G + PF KG
Sbjct: 1 MGKPRGLRTARKHV-NHRRDQRWADKDYKKAHLGTRWKANPFGGASHAKG 49
>AE013599-1845|AAF58277.2| 143|Drosophila melanogaster CG8415-PA
protein.
Length = 143
Score = 121 bits (291), Expect = 8e-28
Identities = 55/61 (90%), Positives = 59/61 (96%)
Frame = +2
Query: 125 KNSTKAHMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAF 304
K+ KAH+GT+WKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK+TAF
Sbjct: 25 KDYKKAHLGTRWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKITAF 84
Query: 305 V 307
V
Sbjct: 85 V 85
Score = 113 bits (271), Expect = 2e-25
Identities = 67/142 (47%), Positives = 77/142 (54%), Gaps = 1/142 (0%)
Frame = +3
Query: 60 PQAIRTARKHVNHRS*TAMGGQRIQRKPTWVR-NGRLTLSVVHLTQRAXXXXXXXXXXXX 236
P+ +RTARKHVNHR + ++ R H +
Sbjct: 4 PRGLRTARKHVNHRRDQRWADKDYKKAHLGTRWKANPFGGASHA--KGIVLEKVGVEAKQ 61
Query: 237 PTLPSANASVYSSLRTERK*PHSSPXDGCLNHIEXNDEXLVAGFGRKGHAVGDIPGVRFK 416
P ++ +K P DG LN+IE NDE LVAGFGRKGHAVGDIPGVRFK
Sbjct: 62 PNSAIRKCVRVQLIKNGKKITAFVPRDGSLNYIEENDEVLVAGFGRKGHAVGDIPGVRFK 121
Query: 417 VVKVANVSLLALYKEKKERPXS 482
VVKVANVSLLALYKEKKERP S
Sbjct: 122 VVKVANVSLLALYKEKKERPRS 143
Score = 30.7 bits (66), Expect = 1.5
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = +1
Query: 49 LGKPRRQYERRVST*TTAREQRWADKEFNESPHGYEMEG*PFRWCISRKG 198
+GKPR R R+QRWADK++ ++ G + PF KG
Sbjct: 1 MGKPRGLRTARKHV-NHRRDQRWADKDYKKAHLGTRWKANPFGGASHAKG 49
>M19494-1|AAA28935.1| 140|Drosophila melanogaster protein (
D.melanogaster tko (technical knockout) locus mRNA
encoding tko gene product,complete cds. ).
Length = 140
Score = 46.0 bits (104), Expect = 4e-05
Identities = 23/55 (41%), Positives = 35/55 (63%)
Frame = +2
Query: 143 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFV 307
H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK++ A++
Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKEMVAYI 95
>BT015958-1|AAV36843.1| 140|Drosophila melanogaster RH56553p
protein.
Length = 140
Score = 46.0 bits (104), Expect = 4e-05
Identities = 23/55 (41%), Positives = 35/55 (63%)
Frame = +2
Query: 143 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFV 307
H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK++ A++
Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKEMVAYI 95
>AL133505-2|CAB65841.1| 140|Drosophila melanogaster
EG:BACH59J11.1,FBgn0003714;tko protein.
Length = 140
Score = 46.0 bits (104), Expect = 4e-05
Identities = 23/55 (41%), Positives = 35/55 (63%)
Frame = +2
Query: 143 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFV 307
H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK++ A++
Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKEMVAYI 95
>AL133505-1|CAB63528.1| 154|Drosophila melanogaster
EG:BACH59J11.1,FBgn0003714;tko protein.
Length = 154
Score = 46.0 bits (104), Expect = 4e-05
Identities = 23/55 (41%), Positives = 35/55 (63%)
Frame = +2
Query: 143 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFV 307
H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK++ A++
Sbjct: 56 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKEMVAYI 109
>AE014298-399|AAF45781.1| 140|Drosophila melanogaster CG7925-PB
protein.
Length = 140
Score = 46.0 bits (104), Expect = 4e-05
Identities = 23/55 (41%), Positives = 35/55 (63%)
Frame = +2
Query: 143 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFV 307
H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+L GK++ A++
Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVRL-STGKEMVAYI 95
>AJ250320-1|CAB65721.1| 140|Drosophila melanogaster technical
knockout protein protein.
Length = 140
Score = 43.2 bits (97), Expect = 3e-04
Identities = 22/55 (40%), Positives = 34/55 (61%)
Frame = +2
Query: 143 HMGTKWKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKKVTAFV 307
H+ T+ P G AKG+VL+ + + K+PNSA RKCV V+ GK++ A++
Sbjct: 42 HIKTRPPRQPLDGKPFAKGVVLKTLIKKPKKPNSANRKCVLVR-HSTGKEMVAYI 95
>AY058686-1|AAL13915.1| 547|Drosophila melanogaster LD40728p
protein.
Length = 547
Score = 30.7 bits (66), Expect = 1.5
Identities = 15/63 (23%), Positives = 32/63 (50%)
Frame = +2
Query: 50 WVNPAGNTNGA*AREPPLVNSDGRTKNSTKAHMGTKWKANPFGGASHAKGIVLEKVGVEA 229
W + + A +++PP+ S+ +++ +K + K KA+ H K + LE ++
Sbjct: 53 WCPDDKDGSPAESKDPPISISEEKSETQSKEVLSEKRKASSTDDEGHVKKVKLEANSLKT 112
Query: 230 KQP 238
K+P
Sbjct: 113 KRP 115
>AE014134-1900|AAF52961.2| 547|Drosophila melanogaster CG6187-PA
protein.
Length = 547
Score = 30.7 bits (66), Expect = 1.5
Identities = 15/63 (23%), Positives = 32/63 (50%)
Frame = +2
Query: 50 WVNPAGNTNGA*AREPPLVNSDGRTKNSTKAHMGTKWKANPFGGASHAKGIVLEKVGVEA 229
W + + A +++PP+ S+ +++ +K + K KA+ H K + LE ++
Sbjct: 53 WCPDDKDGSPAESKDPPISISEEKSETQSKEVLSEKRKASSTDDEGHVKKVKLEANSLKT 112
Query: 230 KQP 238
K+P
Sbjct: 113 KRP 115
>BT016082-1|AAV36967.1| 568|Drosophila melanogaster LD47465p
protein.
Length = 568
Score = 29.1 bits (62), Expect = 4.6
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 497 HNVHL*XWSLLFLFVESEERHVGYFYHLKTNSGNV-TDGV 381
HN++ W + + E R VG F+H KTN+ N+ TDG+
Sbjct: 393 HNINSQVWCQI-AYWEMAHR-VGEFFHAKTNAVNIYTDGI 430
>AY070520-1|AAL47991.1| 507|Drosophila melanogaster GH23534p
protein.
Length = 507
Score = 29.1 bits (62), Expect = 4.6
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 497 HNVHL*XWSLLFLFVESEERHVGYFYHLKTNSGNV-TDGV 381
HN++ W + + E R VG F+H KTN+ N+ TDG+
Sbjct: 332 HNINSQVWCQI-AYWEMAHR-VGEFFHAKTNAVNIYTDGI 369
>AE014297-2300|AAX52959.1| 568|Drosophila melanogaster CG5201-PC,
isoform C protein.
Length = 568
Score = 29.1 bits (62), Expect = 4.6
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 497 HNVHL*XWSLLFLFVESEERHVGYFYHLKTNSGNV-TDGV 381
HN++ W + + E R VG F+H KTN+ N+ TDG+
Sbjct: 393 HNINSQVWCQI-AYWEMAHR-VGEFFHAKTNAVNIYTDGI 430
>AE014297-2299|AAF55383.1| 568|Drosophila melanogaster CG5201-PB,
isoform B protein.
Length = 568
Score = 29.1 bits (62), Expect = 4.6
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 497 HNVHL*XWSLLFLFVESEERHVGYFYHLKTNSGNV-TDGV 381
HN++ W + + E R VG F+H KTN+ N+ TDG+
Sbjct: 393 HNINSQVWCQI-AYWEMAHR-VGEFFHAKTNAVNIYTDGI 430
>AE014297-2298|AAN13728.1| 568|Drosophila melanogaster CG5201-PA,
isoform A protein.
Length = 568
Score = 29.1 bits (62), Expect = 4.6
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 497 HNVHL*XWSLLFLFVESEERHVGYFYHLKTNSGNV-TDGV 381
HN++ W + + E R VG F+H KTN+ N+ TDG+
Sbjct: 393 HNINSQVWCQI-AYWEMAHR-VGEFFHAKTNAVNIYTDGI 430
>AB004232-1|BAA22841.1| 568|Drosophila melanogaster DAD polypeptide
protein.
Length = 568
Score = 29.1 bits (62), Expect = 4.6
Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 1/40 (2%)
Frame = -1
Query: 497 HNVHL*XWSLLFLFVESEERHVGYFYHLKTNSGNV-TDGV 381
HN++ W + + E R VG F+H KTN+ N+ TDG+
Sbjct: 393 HNINSQVWCQI-AYWEMAHR-VGEFFHAKTNAVNIYTDGI 430
>AE014297-650|AAF54148.1| 358|Drosophila melanogaster CG18268-PA
protein.
Length = 358
Score = 28.3 bits (60), Expect = 8.1
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 2/39 (5%)
Frame = +2
Query: 170 PFGGASHAKGIVLEKVGVEAK--QPNSAIRKCVRVQLIK 280
PFG + +VLE+ G K Q IRKCV + +K
Sbjct: 123 PFGNVEYVLKVVLERRGTHNKCFQQRLVIRKCVELADLK 161
Database: fruitfly
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 24,988,368
Number of sequences in database: 53,049
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 27,890,177
Number of Sequences: 53049
Number of extensions: 608364
Number of successful extensions: 1727
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 1507
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1727
length of database: 24,988,368
effective HSP length: 81
effective length of database: 20,691,399
effective search space used: 2338128087
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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