Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_I18
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31020.1 68415.m03782 oxysterol-binding family protein simila... 45 1e-10
At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-contain... 43 3e-09
At1g13170.1 68414.m01527 oxysterol-binding family protein simila... 44 7e-05
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 43 2e-04
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 43 2e-04
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 43 2e-04
At4g12460.1 68417.m01971 oxysterol-binding family protein simila... 42 4e-04
At4g22540.1 68417.m03253 oxysterol-binding family protein simila... 41 6e-04
At2g30880.2 68415.m03764 pleckstrin homology (PH) domain-contain... 36 0.024
At2g30880.1 68415.m03763 pleckstrin homology (PH) domain-contain... 36 0.024
At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3) s... 33 0.17
At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical... 33 0.17
At4g22530.1 68417.m03251 embryo-abundant protein-related similar... 30 1.5
At1g48400.1 68414.m05406 F-box family protein contains F-box dom... 29 3.6
At5g66750.1 68418.m08414 SNF2 domain-containing protein / helica... 28 4.7
At2g29700.1 68415.m03610 pleckstrin homology (PH) domain-contain... 28 4.7
At5g10510.1 68418.m01217 ovule development protein, putative sim... 28 6.2
At1g47810.1 68414.m05319 F-box family protein contains F-box dom... 28 6.2
>At2g31020.1 68415.m03782 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 760
Score = 44.8 bits (101), Expect(2) = 1e-10
Identities = 20/49 (40%), Positives = 29/49 (59%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGPXDTIQPGQPARG 282
++G LYK+ N KGW+ RWF + + GVLSYY GP + + +G
Sbjct: 73 IAGILYKWVNYGKGWRPRWFVL--QDGVLSYYKIKGPDKIVVIHETEKG 119
Score = 38.7 bits (86), Expect(2) = 1e-10
Identities = 30/115 (26%), Positives = 51/115 (44%)
Frame = +1
Query: 280 GEAHLXAAVICPXDEXSKTFTINCAXGDMLXLRATDARARQEWVDGLRAIAXIHTKVMGA 459
GE HL + I K F+I L LRA R+ W++ L+A+ + ++
Sbjct: 153 GEVHLKVSSIRESRSDDKRFSIFTGT-KRLHLRAETREDREAWIEALQAVKDMFPRMSNC 211
Query: 460 NPPLQPREQLAVHDAMASARQQLQATELSDAALARCIESSDSPFPHTDPDLLLLK 624
+ P L + ++ R +L +S++A+ C + + S F LLLLK
Sbjct: 212 E-LMAPTNNLDI--SIEKLRLRLVEEGVSESAIQDCEQITRSEFSAIQSQLLLLK 263
>At1g77730.1 68414.m09050 pleckstrin homology (PH) domain-containing
protein similar to SP|P16258 Oxysterol-binding protein 1
{Oryctolagus cuniculus}; contains Pfam profile PF00169:
PH domain
Length = 265
Score = 43.2 bits (97), Expect(2) = 3e-09
Identities = 18/37 (48%), Positives = 26/37 (70%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGP 246
++G LYK+ N +GW++RWF + + GVLSYY GP
Sbjct: 54 VAGILYKWVNYGQGWKRRWFVL--QDGVLSYYRIHGP 88
Score = 35.5 bits (78), Expect(2) = 3e-09
Identities = 30/125 (24%), Positives = 52/125 (41%)
Frame = +1
Query: 250 DTIQPGQPARGEAHLXAAVICPXDEXSKTFTINCAXGDMLXLRATDARARQEWVDGLRAI 429
D PG+P G+ HL + I K FT+ L LRA + R W++ L+A+
Sbjct: 120 DVHSPGKPL-GQIHLKVSSIGQSISDGKRFTVFTGTKS-LHLRAATSEDRASWIEALKAV 177
Query: 430 AXIHTKVMGANPPLQPREQLAVHDAMASARQQLQATELSDAALARCIESSDSPFPHTDPD 609
++ +N L V + RQ+L E+ + + C + + F +
Sbjct: 178 KETFPRM--SNEELM-ASTTNVSVSTDKLRQRLMEEEVDETIIKDCEDIMKNNFLALHDE 234
Query: 610 LLLLK 624
++ LK
Sbjct: 235 VMSLK 239
>At1g13170.1 68414.m01527 oxysterol-binding family protein similar
to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus
cuniculus}; contains Pfam profiles PF00169: PH domain,
PF01237: Oxysterol-binding protein
Length = 816
Score = 44.4 bits (100), Expect = 7e-05
Identities = 20/49 (40%), Positives = 28/49 (57%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGPXDTIQPGQPARG 282
++G +YK+ N KGW+ RWF + E GVLSYY GP + +G
Sbjct: 95 VAGIMYKWVNYGKGWRARWFEL--EDGVLSYYKIHGPDKIVMNPSREKG 141
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 43.2 bits (97), Expect = 2e-04
Identities = 18/37 (48%), Positives = 25/37 (67%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGP 246
++G LYK+ N +GW+ RWF + + GVLSYY GP
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVL--QDGVLSYYKIHGP 140
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 43.2 bits (97), Expect = 2e-04
Identities = 18/37 (48%), Positives = 25/37 (67%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGP 246
++G LYK+ N +GW+ RWF + + GVLSYY GP
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVL--QDGVLSYYKIHGP 140
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 43.2 bits (97), Expect = 2e-04
Identities = 18/37 (48%), Positives = 25/37 (67%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGP 246
++G LYK+ N +GW+ RWF + + GVLSYY GP
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVL--QDGVLSYYKIHGP 140
>At4g12460.1 68417.m01971 oxysterol-binding family protein similar
to SP|P22059 Oxysterol-binding protein 1 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 694
Score = 41.9 bits (94), Expect = 4e-04
Identities = 19/37 (51%), Positives = 25/37 (67%)
Frame = +1
Query: 118 LTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 228
L S R ++G LYK+TN KGW+ RWF + G+LSY
Sbjct: 22 LQSGRSVAGILYKWTNYGKGWRSRWFLL--RDGILSY 56
>At4g22540.1 68417.m03253 oxysterol-binding family protein similar
to SP|P16258 Oxysterol-binding protein 1 {Oryctolagus
cuniculus}; contains Pfam profiles PF00169: PH domain,
PF01237: Oxysterol-binding protein
Length = 721
Score = 41.1 bits (92), Expect = 6e-04
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +1
Query: 112 TGLTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 228
TG + ++G LYK+TN KGW+ RWF + G+LSY
Sbjct: 45 TGGSESETVAGILYKWTNFGKGWRSRWFLL--RNGILSY 81
>At2g30880.2 68415.m03764 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 381
Score = 35.9 bits (79), Expect = 0.024
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 228
L G L+K + ++ W +RW +DP TG + Y
Sbjct: 34 LQGPLFKRSETLRKWNERWVILDPTTGKMEY 64
>At2g30880.1 68415.m03763 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 504
Score = 35.9 bits (79), Expect = 0.024
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +1
Query: 136 LSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 228
L G L+K + ++ W +RW +DP TG + Y
Sbjct: 34 LQGPLFKRSETLRKWNERWVILDPTTGKMEY 64
>At1g59610.1 68414.m06704 dynamin-like protein, putative (ADL3)
strong similarity to dynamin-like protein 6 (ADL6)
[Arabidopsis thaliana] GI:6651399; contains Pfam
profiles PF01031: Dynamin central region, PF00350:
Dynamin family, PF02212: Dynamin GTPase effector domain,
PF00169: PH domain; identical to cDNA dynamin-like
protein ADL3, GI:4803835
Length = 920
Score = 33.1 bits (72), Expect = 0.17
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +1
Query: 106 MKTGLTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 228
+KT +G L K + GW +RWF ++ +TG L Y
Sbjct: 572 LKTAGPEGEITAGYLMKKSAKTNGWSRRWFVLNEKTGKLGY 612
>At1g10290.1 68414.m01159 dynamin-like protein 6 (ADL6) identical to
dynamin-like protein 6 (ADL6) [Arabidopsis thaliana]
GI:6651399; contains Pfam profiles PF01031: Dynamin
central region, PF00350: Dynamin family, PF02212:
Dynamin GTPase effector domain, PF00169: PH domain
Length = 914
Score = 33.1 bits (72), Expect = 0.17
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +1
Query: 106 MKTGLTSHRPLSGQLYKYTNVVKGWQQRWFAVDPETGVLSY 228
+KT +G L K + GW +RWF ++ +TG L Y
Sbjct: 565 LKTAGPEGEITAGYLMKKSAKTNGWSRRWFVLNEKTGKLGY 605
>At4g22530.1 68417.m03251 embryo-abundant protein-related similar to
embryo-abundant protein [Picea glauca] GI:1350531
Length = 261
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +3
Query: 210 DWCSILLSL*WTXRHNTAWTAGQGGG 287
DW S L +L + RHN AW AG G G
Sbjct: 22 DWYSKLAAL--SHRHNLAWDAGTGNG 45
>At1g48400.1 68414.m05406 F-box family protein contains F-box domain
Pfam:PF00646
Length = 513
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +1
Query: 145 QLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGP 246
++YKY + V G +RW E G+L +L+ P
Sbjct: 97 RVYKYNDDVDGMVRRWIRTVMERGLLEIHLHATP 130
>At5g66750.1 68418.m08414 SNF2 domain-containing protein / helicase
domain-containing protein similar to
proliferation-associated SNF2-like protein [Homo
sapiens] GI:8980660; contains Pfam profiles PF00271:
Helicase conserved C-terminal domain, PF00176: SNF2
family N-terminal domain
Length = 764
Score = 28.3 bits (60), Expect = 4.7
Identities = 15/48 (31%), Positives = 27/48 (56%)
Frame = +1
Query: 439 HTKVMGANPPLQPREQLAVHDAMASARQQLQATELSDAALARCIESSD 582
H + ++ PL+ + LA+ +A +L T++SDA L R ++ SD
Sbjct: 679 HQERAKSSTPLEEEDILALLKEDETAEDKLIQTDISDADLDRLLDRSD 726
>At2g29700.1 68415.m03610 pleckstrin homology (PH) domain-containing
protein (PH1) identical to AtPH1 [Arabidopsis thaliana]
GI:5926716; contains Pfam profile PF00169: PH domain
Length = 145
Score = 28.3 bits (60), Expect = 4.7
Identities = 25/106 (23%), Positives = 47/106 (44%), Gaps = 3/106 (2%)
Frame = +1
Query: 139 SGQLYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGPXDTIQPGQPARGEAHL--XAAVIC 312
SG L K + +K W++RWF + + G L ++ D I+ G RG + V
Sbjct: 30 SGWLTKQGDYIKTWRRRWFVL--KRGKLLWF-KDQAAAGIR-GSTPRGVISVGDCLTVKG 85
Query: 313 PXDEXSKTFTINCAXGD-MLXLRATDARARQEWVDGLRAIAXIHTK 447
D +K F + G + A + + ++EW++ + H++
Sbjct: 86 AEDVVNKPFAFELSSGSYTMFFIADNEKEKEEWINSIGRSIVQHSR 131
>At5g10510.1 68418.m01217 ovule development protein, putative
similar to ovule development protein aintegumenta
(GI:1209099) [Arabidopsis thaliana]
Length = 566
Score = 27.9 bits (59), Expect = 6.2
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = -3
Query: 241 HHKDNKIEHQSQDQQQTIFAAIPS 170
HH + +HQ Q QQQ F P+
Sbjct: 506 HHHQQQQQHQQQQQQQNFFQHFPA 529
>At1g47810.1 68414.m05319 F-box family protein contains F-box domain
Pfam:PF00646
Length = 351
Score = 27.9 bits (59), Expect = 6.2
Identities = 14/41 (34%), Positives = 22/41 (53%)
Frame = +1
Query: 148 LYKYTNVVKGWQQRWFAVDPETGVLSYYLYDGPXDTIQPGQ 270
L+ + N +KG+Q+ W+ T V S LY+ P + P Q
Sbjct: 68 LFAFKNQIKGYQENWYFFSKSTHV-STPLYE-PNSLVLPDQ 106
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,263,554
Number of Sequences: 28952
Number of extensions: 228420
Number of successful extensions: 564
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 541
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 558
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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