Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_H20
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein S3a|S... 163 2e-41
SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein S3a|... 161 8e-41
SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomy... 28 1.0
SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 28 1.4
SPBC106.11c |plg7||phospholipase A2 |Schizosaccharomyces pombe|c... 27 2.4
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 27 3.1
SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 26 5.5
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 5.5
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 25 9.5
>SPAC13G6.02c |rps101|rps1-1, rps3a-1|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 163 bits (397), Expect = 2e-41
Identities = 79/156 (50%), Positives = 111/156 (71%)
Frame = +2
Query: 182 KIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRW 361
K A++ LKGR+ EVSLADLQ D E SFRK +L E +QG++ L +F+G D+T+DKLR
Sbjct: 55 KNANDSLKGRILEVSLADLQKDE--EHSFRKVKLRVEDIQGKSCLTSFNGFDMTSDKLRS 112
Query: 362 MVKKWQTLIEANIDVXTTDGYVLRVFCIGFTNKDSLSQRKTCYAQHTQVRAIXKKMCEII 541
+V+KWQ+ IEAN + TTDGY+ R+F IGFT++ +KT YAQ +Q+RAI +KM ++I
Sbjct: 113 LVRKWQSTIEANQTIKTTDGYLCRIFVIGFTSRRVNQVKKTTYAQSSQIRAIHQKMFQVI 172
Query: 542 TRDVTNSELREVVNKLIPDSIAKDIEKACHGIYPLR 649
++E+V KLIP+ I + IEKA + IYPL+
Sbjct: 173 QNQANGCSMKELVQKLIPEVIGRAIEKATNNIYPLQ 208
Score = 66.5 bits (155), Expect = 3e-12
Identities = 30/62 (48%), Positives = 42/62 (67%)
Frame = +1
Query: 25 GKIKACRRAVKKVLTKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQXNENCFGRIE 204
GK K + KK + K++VDPF+RKDWYD+KAP+ F + VG TLVNRT +N ++
Sbjct: 4 GKNKRLSKG-KKGIKKRVVDPFSRKDWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLK 62
Query: 205 GK 210
G+
Sbjct: 63 GR 64
>SPAC22H12.04c |rps102|rps1-2, rps3a-2|40S ribosomal protein
S3a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 252
Score = 161 bits (391), Expect = 8e-41
Identities = 77/156 (49%), Positives = 113/156 (72%)
Frame = +2
Query: 182 KIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRW 361
K A++ LKGR+ EVSLADLQ D E +FRK +L E +QG++ L +F+G+ +T+DKLR
Sbjct: 55 KNANDSLKGRILEVSLADLQKDE--EHAFRKVKLRVEDIQGKSCLTSFNGLSITSDKLRS 112
Query: 362 MVKKWQTLIEANIDVXTTDGYVLRVFCIGFTNKDSLSQRKTCYAQHTQVRAIXKKMCEII 541
+V+KWQT IEA+ + TTDGY+ RVF IGFT + + +KT YAQ +Q+RAI +KM ++I
Sbjct: 113 LVRKWQTTIEADQTIKTTDGYLCRVFVIGFTRRRANQVKKTTYAQSSQIRAIRQKMFQVI 172
Query: 542 TRDVTNSELREVVNKLIPDSIAKDIEKACHGIYPLR 649
++ +RE+V KLIP+ I ++IE+A I+PL+
Sbjct: 173 QNQTSSCSMRELVQKLIPEVIGREIERATGSIFPLQ 208
Score = 64.9 bits (151), Expect = 1e-11
Identities = 29/62 (46%), Positives = 42/62 (67%)
Frame = +1
Query: 25 GKIKACRRAVKKVLTKKIVDPFTRKDWYDVKAPSMFSKRQVGTTLVNRTQXNENCFGRIE 204
GK K + KK + K++VDPF+RK+WYD+KAP+ F + VG TLVNRT +N ++
Sbjct: 4 GKNKRLSKG-KKGIKKRVVDPFSRKEWYDIKAPAFFEVKNVGKTLVNRTAGLKNANDSLK 62
Query: 205 GK 210
G+
Sbjct: 63 GR 64
>SPBP8B7.19 |spt16||FACT complex component Spt16|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1019
Score = 28.3 bits (60), Expect = 1.0
Identities = 24/102 (23%), Positives = 43/102 (42%), Gaps = 1/102 (0%)
Frame = +2
Query: 179 TKIASEGLKGRVFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLR 358
TK+A KG +V++ L+ DAE + + F I EY++ N D T K
Sbjct: 91 TKLAET--KGAAADVNI--LKRTKDAEENKKLFEKIIEYIRATNKKVGVFPKDKTQGK-- 144
Query: 359 WMVKKWQTLIE-ANIDVXTTDGYVLRVFCIGFTNKDSLSQRK 481
+ +W ++ E + D + C+ ++ L+ K
Sbjct: 145 -FINEWDSIFEPVKSEFNLVDASLGLAKCLAIKDEQELANIK 185
>SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 308
Score = 27.9 bits (59), Expect = 1.4
Identities = 22/70 (31%), Positives = 35/70 (50%), Gaps = 2/70 (2%)
Frame = +2
Query: 212 VFEVSLADLQADTDAERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMV--KKWQTL 385
VF+V + + D R+ + +A Q R V C H MDL + LR +V ++ L
Sbjct: 86 VFDVESREFERDLVTFRNCLE-ATVANSPQAR-VFCLIHKMDLVQEDLRDLVFEERKAIL 143
Query: 386 IEANIDVXTT 415
+E + D+ TT
Sbjct: 144 LETSKDLETT 153
>SPBC106.11c |plg7||phospholipase A2 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 438
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/56 (32%), Positives = 27/56 (48%)
Frame = -3
Query: 273 LRKDLSASVSACRSARETSKTLPFNPSEAIFVALGTVDKRGADLPLAEHRRSLDIV 106
+ KD S + +A SA E +P +FV GTV +DLP+ R L ++
Sbjct: 332 VNKD-SENQNAGESADEKMSVVPLKKYSHVFVYDGTVHANQSDLPILLPRMVLRVL 386
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = -2
Query: 379 LPFFNHPPELIGCEVHAVEVAEHITS 302
L H PEL+ C +VA+H+TS
Sbjct: 181 LQSMGHSPELVQCITCLPDVADHLTS 206
>SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 882
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +2
Query: 356 RWMVKKWQTLIEANIDVXTTDGYVLRVFCIGFTNKDSLSQRKTCYA 493
++ W L+ T GYV+R GFT+ ++ + K +A
Sbjct: 795 KFATSNWDKLLSRLPVAGTMRGYVVRFVTSGFTHASAIDKIKEFFA 840
>SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1309
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/33 (36%), Positives = 18/33 (54%), Gaps = 2/33 (6%)
Frame = +1
Query: 439 LHWFHQ*GLLEPTQDVLRPAHSGQ--SNXKENV 531
LHW+ L+ +Q + P H+GQ S + NV
Sbjct: 1162 LHWYSLMSLMGTSQGICIPRHAGQVLSTPRRNV 1194
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -2
Query: 358 PELIGCEVHAVEVAEHITSL 299
PELI C H EV E I SL
Sbjct: 216 PELINCIAHPEEVPETIHSL 235
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,691,775
Number of Sequences: 5004
Number of extensions: 55332
Number of successful extensions: 157
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 149
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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