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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P03_F_H11
         (649 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g27380.1 68418.m03269 glutathione synthetase (GSH2) non-conse...    72   4e-13
At4g11550.1 68417.m01852 DC1 domain-containing protein contains ...    32   0.29 
At4g38600.2 68417.m05463 HECT-domain-containing protein / ubiqui...    31   0.66 
At4g38600.1 68417.m05464 HECT-domain-containing protein / ubiqui...    31   0.66 
At5g37060.1 68418.m04448 cation/hydrogen exchanger, putative (CH...    30   1.2  
At5g14400.1 68418.m01682 cytochrome P450 family protein similar ...    30   1.5  
At5g58460.1 68418.m07321 cation/hydrogen exchanger, putative (CH...    29   2.0  
At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putat...    29   3.5  
At4g11390.1 68417.m01837 DC1 domain-containing protein contains ...    28   4.7  

>At5g27380.1 68418.m03269 glutathione synthetase (GSH2)
           non-consensus AT donor splice site at exon 6, AC
           acceptor splice site at exon 7; identical to
           Swiss-Prot:P46416 glutathione synthetase, chloroplast
           precursor (Glutathione synthase) (GSH synthetase)
           (GSH-S) [Arabidopsis thaliana]
          Length = 478

 Score = 71.7 bits (168), Expect = 4e-13
 Identities = 39/126 (30%), Positives = 69/126 (54%), Gaps = 5/126 (3%)
 Frame = +2

Query: 263 LENKTLVNVIEKAXDWALMHGVGMRDKKHFNKDVI-----QIAPFILLPSPFPKTEFMKA 427
           L+++ +  ++  A  W+ +HG+ + DK +     +       AP  LLP+ FP+  + +A
Sbjct: 13  LDDEFVQKLVYDALVWSSLHGLVVGDKSYQKSGNVPGVGLMHAPIALLPTAFPEAYWKQA 72

Query: 428 VELQPVLNELMHKVAHDDEFLEQTLQNALQVDEFTANLYDIWLKVKNEGISQPISLGMLR 607
             + P+ NEL+ +V+ D +FL+ +L    +VD FT+ L DI  K+      + I LG+ R
Sbjct: 73  CNVTPLFNELIDRVSLDGKFLQDSLSRTKKVDVFTSRLLDIHSKMLERNKKEDIRLGLHR 132

Query: 608 SDIMLE 625
            D ML+
Sbjct: 133 FDYMLD 138


>At4g11550.1 68417.m01852 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 668

 Score = 32.3 bits (70), Expect = 0.29
 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
 Frame = -2

Query: 648 FSVCXHLDSSMMSERSIPSDIG*DMP-SFFTFNHMSYKFAVNSSTCRAFCKVCSKN 484
           F  C    +S+ + + +  D    MP S  T++H+SY+   N+S  R FC+ C  +
Sbjct: 572 FYTCKDHRASLHT-KCVMGDFARLMPGSTLTYSHISYEVVRNNSMSRPFCRACESH 626


>At4g38600.2 68417.m05463 HECT-domain-containing protein /
            ubiquitin-transferase family protein similar to
            SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
            {Homo sapiens}; contains Pfam profile PF00632:
            HECT-domain (ubiquitin-transferase)
          Length = 1794

 Score = 31.1 bits (67), Expect = 0.66
 Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
 Frame = +2

Query: 353  NKDVIQIAPFILLPSPFPKT-------EFMKAVELQPVLNELMHKVAHDDEFLEQTLQNA 511
            N+D++ +AP  L P P+P T       +F K +E   +L  +M K   D   L+  L  A
Sbjct: 1467 NRDIV-LAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTA 1525


>At4g38600.1 68417.m05464 HECT-domain-containing protein /
            ubiquitin-transferase family protein similar to
            SP|Q14669Thyroid receptor interacting protein 12 (TRIP12)
            {Homo sapiens}; contains Pfam profile PF00632:
            HECT-domain (ubiquitin-transferase)
          Length = 1888

 Score = 31.1 bits (67), Expect = 0.66
 Identities = 19/60 (31%), Positives = 29/60 (48%), Gaps = 7/60 (11%)
 Frame = +2

Query: 353  NKDVIQIAPFILLPSPFPKT-------EFMKAVELQPVLNELMHKVAHDDEFLEQTLQNA 511
            N+D++ +AP  L P P+P T       +F K +E   +L  +M K   D   L+  L  A
Sbjct: 1558 NRDIV-LAPLGLFPRPWPSTADISEGGQFHKVIEYFRLLGRVMAKALQDGRLLDVPLSTA 1616


>At5g37060.1 68418.m04448 cation/hydrogen exchanger, putative
           (CHX24) similar to Na+/H+-exchanging protein NapA -
           Enterococcus hirae, PIR:A42111; monovalent cation:proton
           antiporter family 2 (CPA2) member, PMID:11500563
          Length = 859

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 13/53 (24%), Positives = 26/53 (49%)
 Frame = +2

Query: 443 VLNELMHKVAHDDEFLEQTLQNALQVDEFTANLYDIWLKVKNEGISQPISLGM 601
           V NE   +V++ +  ++   +    +     N YD+W+  + EGI+  I  G+
Sbjct: 745 VKNESNERVSYKEVVVKNGAETLAAIQAMNVNDYDLWITGRREGINPKILEGL 797


>At5g14400.1 68418.m01682 cytochrome P450 family protein similar to
           steroid 22-alpha-hydroxylase; DWF4; CYP90B1 (GI:2935342)
           [Arabidopsis thaliana]
          Length = 439

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 14/50 (28%), Positives = 28/50 (56%)
 Frame = +2

Query: 380 FILLPSPFPKTEFMKAVELQPVLNELMHKVAHDDEFLEQTLQNALQVDEF 529
           FI LP P P T +  A++ +  L+  +  +  + E  E+ + NA++ ++F
Sbjct: 173 FISLPIPLPGTGYTNAIKARKRLSARVMGMIKEREREEEDMNNAIREEDF 222


>At5g58460.1 68418.m07321 cation/hydrogen exchanger, putative
           (CHX25) similar to Na+/H+-exchanging protein slr1595,
           Synechocystis sp., PIR:S74951; monovalent cation:proton
           antiporter family 2 (CPA2) member, PMID:11500563
          Length = 857

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 13/53 (24%), Positives = 26/53 (49%)
 Frame = +2

Query: 443 VLNELMHKVAHDDEFLEQTLQNALQVDEFTANLYDIWLKVKNEGISQPISLGM 601
           V NE   +V++ +  ++   +    +     N YD+W+  + EGI+  I  G+
Sbjct: 743 VKNESNARVSYKEVVVKNGAETLAAIQAMNVNDYDLWITGRREGINPKILEGL 795


>At4g26970.1 68417.m03881 aconitate hydratase, cytoplasmic, putative
           / citrate hydro-lyase/aconitase, putative strong
           similarity to SP|P49608 Aconitate hydratase, cytoplasmic
           (EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
           {Cucurbita maxima}; contains Pfam profiles PF00330:
           Aconitase family (aconitate hydratase), PF00694:
           Aconitase C-terminal domain
          Length = 995

 Score = 28.7 bits (61), Expect = 3.5
 Identities = 16/44 (36%), Positives = 25/44 (56%)
 Frame = +2

Query: 326 VGMRDKKHFNKDVIQIAPFILLPSPFPKTEFMKAVELQPVLNEL 457
           V +RD    N++V Q+  + +LPS F K+ +    E  P+ NEL
Sbjct: 685 VYLRDVWPSNEEVAQVVQYSVLPSMF-KSSYETITEGNPLWNEL 727


>At4g11390.1 68417.m01837 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 514

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 12/32 (37%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
 Frame = -2

Query: 576 MP-SFFTFNHMSYKFAVNSSTCRAFCKVCSKN 484
           MP S   +   SY+  +N+S  R FC+VC  +
Sbjct: 370 MPRSTIKYGSRSYEVVLNNSVSRPFCRVCESH 401


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,899,112
Number of Sequences: 28952
Number of extensions: 218465
Number of successful extensions: 628
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 623
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 628
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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