Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_G11
(464 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 101 5e-23
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 80 1e-16
SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 80 2e-16
SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 80 2e-16
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 80 2e-16
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 80 2e-16
SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 37 0.001
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 37 0.001
SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 37 0.002
SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 25 7.5
>SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like
protein modifier Ned8|Schizosaccharomyces pombe|chr
2|||Manual
Length = 78
Score = 101 bits (242), Expect = 5e-23
Identities = 46/63 (73%), Positives = 54/63 (85%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
MLIKVKTLTGKEIE+DI+P DKV RIK RV EKEGIPP QQRLI++GKQM D+K A+ Y
Sbjct: 1 MLIKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYH 60
Query: 318 VQG 326
++G
Sbjct: 61 LEG 63
Score = 29.5 bits (63), Expect = 0.26
Identities = 13/13 (100%), Positives = 13/13 (100%)
Frame = +1
Query: 328 GSVLHLVLALRGG 366
GSVLHLVLALRGG
Sbjct: 64 GSVLHLVLALRGG 76
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 80.2 bits (189), Expect = 1e-16
Identities = 35/81 (43%), Positives = 53/81 (65%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 318 VQGRFSTSSCFGFKGRRMIWL 380
+Q + +G I++
Sbjct: 61 IQKESTLHLVLRLRGGMQIFV 81
Score = 80.2 bits (189), Expect = 1e-16
Identities = 35/81 (43%), Positives = 53/81 (65%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136
Query: 318 VQGRFSTSSCFGFKGRRMIWL 380
+Q + +G I++
Sbjct: 137 IQKESTLHLVLRLRGGMQIFV 157
Score = 80.2 bits (189), Expect = 1e-16
Identities = 35/81 (43%), Positives = 53/81 (65%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212
Query: 318 VQGRFSTSSCFGFKGRRMIWL 380
+Q + +G I++
Sbjct: 213 IQKESTLHLVLRLRGGMQIFV 233
Score = 80.2 bits (189), Expect = 1e-16
Identities = 35/81 (43%), Positives = 53/81 (65%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288
Query: 318 VQGRFSTSSCFGFKGRRMIWL 380
+Q + +G I++
Sbjct: 289 IQKESTLHLVLRLRGGMQIFV 309
Score = 79.8 bits (188), Expect = 2e-16
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364
Query: 318 VQ 323
+Q
Sbjct: 365 IQ 366
>SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein
Ubi2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 79.8 bits (188), Expect = 2e-16
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 318 VQ 323
+Q
Sbjct: 61 IQ 62
>SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein
Ubi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 128
Score = 79.8 bits (188), Expect = 2e-16
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 318 VQ 323
+Q
Sbjct: 61 IQ 62
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 79.8 bits (188), Expect = 2e-16
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 318 VQ 323
+Q
Sbjct: 61 IQ 62
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 79.8 bits (188), Expect = 2e-16
Identities = 33/62 (53%), Positives = 47/62 (75%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYK 317
M I VKTLTGK I +++E +D ++ +K ++ +KEGIPP QQRLIF+GKQ+ D +T DY
Sbjct: 1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 318 VQ 323
+Q
Sbjct: 61 IQ 62
>SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase
Ubp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 37.1 bits (82), Expect = 0.001
Identities = 13/44 (29%), Positives = 28/44 (63%)
Frame = +3
Query: 165 GKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDE 296
GK+ +++IEP + +K ++ +PP++Q++I G Q+ D+
Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDD 53
>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 368
Score = 37.1 bits (82), Expect = 0.001
Identities = 16/63 (25%), Positives = 36/63 (57%), Gaps = 1/63 (1%)
Frame = +3
Query: 138 MLIKVKTLTGKEIEI-DIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDY 314
M + K L ++ I D+ K+ +K ++ ++ ++Q+LI+SG+ + D+KT +Y
Sbjct: 1 MNLTFKNLQQQKFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEY 60
Query: 315 KVQ 323
++
Sbjct: 61 NIK 63
>SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 354
Score = 36.7 bits (81), Expect = 0.002
Identities = 14/60 (23%), Positives = 33/60 (55%)
Frame = +3
Query: 144 IKVKTLTGKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYKVQ 323
+ +K ++ + ++ V +K + I ++QRLI++G+ + DE++ + YK+Q
Sbjct: 6 LTIKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYKIQ 65
>SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier
Hub1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 73
Score = 24.6 bits (51), Expect = 7.5
Identities = 15/57 (26%), Positives = 23/57 (40%)
Frame = +3
Query: 165 GKEIEIDIEPTDKVERIKXRVXEKEGIPPQQQRLIFSGKQMNDEKTAQDYKVQGRFS 335
GK++ + P D V K V + G P++ L D T DY++ S
Sbjct: 11 GKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKWHSVFKDNITLADYEIHDGMS 67
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,621,517
Number of Sequences: 5004
Number of extensions: 29202
Number of successful extensions: 87
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 73
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 83
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 176367270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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