Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_G04
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC83.14c |rfc5||DNA replication factor C complex subunit Rfc5 ... 226 2e-60
SPAC27E2.10c |rfc3|SPAPJ698.01c|DNA replication factor C complex... 97 1e-21
SPAC23D3.02 |rfc2||DNA replication factor C complex subunit Rfc2... 91 2e-19
SPAC1687.03c |rfc4||DNA replication factor C complex subunit Rfc... 79 6e-16
SPAC14C4.13 |rad17||RFC related checkpoint protein Rad17|Schizos... 55 8e-09
SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces p... 27 2.4
SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces pomb... 26 4.1
SPBP23A10.13 |orc4|orp4|origin recognition complex subunit Orc4|... 26 5.5
SPAC9E9.04 |||bcap family homolog|Schizosaccharomyces pombe|chr ... 26 5.5
SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces pom... 26 5.5
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 5.5
SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2... 26 5.5
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 25 9.5
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 25 9.5
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 25 9.5
SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces po... 25 9.5
SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces pombe... 25 9.5
>SPBC83.14c |rfc5||DNA replication factor C complex subunit Rfc5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 358
Score = 226 bits (553), Expect = 2e-60
Identities = 105/185 (56%), Positives = 143/185 (77%)
Frame = +3
Query: 81 LWVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGS 260
LW+D++RPK L LDYHK+ + RL +L ++FPHLL+YGPSGAGKKTRV+ +LRELYG
Sbjct: 2 LWLDQYRPKTLASLDYHKQLSERLISLSSTNEFPHLLVYGPSGAGKKTRVVAILRELYGP 61
Query: 261 GVERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQID 440
G E+L+ + F TPS+KK++I VSS +H+E+ P+DVG +DRV++ +L+K+VAQ+ Q+D
Sbjct: 62 GSEKLKIDQRTFLTPSSKKLQINIVSSLHHLEITPSDVGNYDRVIMQELLKDVAQSAQVD 121
Query: 441 SNGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRCLTI 620
++ FKVV++N D+LT+DAQ ALRRTMEKY RLILIANS S++I IRSR L +
Sbjct: 122 LQAKKIFKVVVINVADELTRDAQAALRRTMEKYSNNIRLILIANSTSKIIEPIRSRTLMV 181
Query: 621 SVPAP 635
V AP
Sbjct: 182 RVAAP 186
>SPAC27E2.10c |rfc3|SPAPJ698.01c|DNA replication factor C complex
subunit Rfc3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 342
Score = 97.5 bits (232), Expect = 1e-21
Identities = 61/190 (32%), Positives = 100/190 (52%), Gaps = 1/190 (0%)
Frame = +3
Query: 84 WVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGSG 263
WV+K+RP +L + HK+ L+ + + PH+L YGP G GK + ++ R++YG
Sbjct: 25 WVEKYRPANLEDVVSHKDIISTLEKFISSNRVPHMLFYGPPGTGKTSTILACARKIYGP- 83
Query: 264 VERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQIDS 443
N + ++M ++++ D GI + + +KN A T QI +
Sbjct: 84 ---------------NYRNQLMELNAS-------DDRGID---AVREQIKNFASTRQIFA 118
Query: 444 NGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRCLTIS 623
+ FK++IL+E D +T AQ+ALRR +EKY R +I N I+++ PAI+SRC
Sbjct: 119 S---TFKMIILDEADAMTLAAQNALRRVIEKYTKNVRFCIICNYINKISPAIQSRCTRFR 175
Query: 624 V-PAPPXNLK 650
P PP ++
Sbjct: 176 FQPLPPKEIE 185
>SPAC23D3.02 |rfc2||DNA replication factor C complex subunit
Rfc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 90.6 bits (215), Expect = 2e-19
Identities = 61/180 (33%), Positives = 94/180 (52%), Gaps = 4/180 (2%)
Frame = +3
Query: 84 WVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGSG 263
WV+ +RPK L ++ + LK + ++ PH+L YG G GK + ++ L REL+G
Sbjct: 21 WVELYRPKTLDQVSSQESTVQVLKKTLLSNNLPHMLFYGSPGTGKTSTILALSRELFG-- 78
Query: 264 VERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDV-GIHDRVVIMDLVKNVAQT---H 431
P K ++ E+N +D GI +I + VK+ A+T +
Sbjct: 79 -------------PQLMKSRVL--------ELNASDERGIS---IIREKVKSFAKTTVTN 114
Query: 432 QIDSNGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRC 611
++D FK++IL+E D +T+DAQ ALRRTME Y R LI N ++R+I + SRC
Sbjct: 115 KVDGYPCPPFKIIILDEADSMTQDAQAALRRTMESYARITRFCLICNYMTRIIDPLSSRC 174
>SPAC1687.03c |rfc4||DNA replication factor C complex subunit
Rfc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 342
Score = 79.0 bits (186), Expect = 6e-16
Identities = 60/176 (34%), Positives = 89/176 (50%)
Frame = +3
Query: 84 WVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGSG 263
WV+K+RP L + ++E RLK + ++ + PHL+I G G GK T ++CL L G
Sbjct: 23 WVEKYRPIVLDDIVGNEETIDRLKVIAKEGNMPHLVISGMPGIGKTTSILCLAHALLG-- 80
Query: 264 VERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQIDS 443
P+ K+ + E+N +D D V+ + +K AQ I
Sbjct: 81 -------------PAYKEGVL---------ELNASDERGID--VVRNRIKAFAQKKVILP 116
Query: 444 NGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRC 611
G+ K++IL+E D +T AQ ALRRTME Y T R L N +++I I+SRC
Sbjct: 117 PGRH--KIIILDEADSMTAGAQQALRRTMEIYSNTTRFALACNQSNKIIEPIQSRC 170
>SPAC14C4.13 |rad17||RFC related checkpoint protein
Rad17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 606
Score = 55.2 bits (127), Expect = 8e-09
Identities = 41/145 (28%), Positives = 73/145 (50%), Gaps = 2/145 (1%)
Frame = +3
Query: 72 KMSLWVDKHRPKDLMKLDYHKEQAVRLKN--LVQQSDFPHLLIYGPSGAGKKTRVMCLLR 245
+ LW +K+ P+ L HK + +K L + LLI GPSG GK T V L +
Sbjct: 68 RFQLWFEKYIPQKAADLAVHKSKISAIKQWMLTDSLESRLLLICGPSGCGKSTAVQVLAK 127
Query: 246 ELYGSGVERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQ 425
EL S +E L MN PSN++ + ++++ + ++ + + + +MD N Q
Sbjct: 128 ELGYSLIEWL--NPMNLKEPSNQESDTLSLTEKFSRFMSLCET--YPELELMD--SNNIQ 181
Query: 426 THQIDSNGQREFKVVILNEVDDLTK 500
++ G+++F + L+E+ L+K
Sbjct: 182 KRGKNAQGKKKF--IFLDEIPHLSK 204
>SPBC21C3.20c |git1||C2 domain protein Git1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1098
Score = 27.1 bits (57), Expect = 2.4
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 378 IHDRVVIMDLVKNVAQTHQIDSNGQREFKVVI 473
+H R V +LV N +T+Q++ + FK++I
Sbjct: 824 VHTRDVYDELVPNWKETYQMEITNVKRFKLII 855
>SPAC57A10.05c |pof1||F-box protein Pof1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 605
Score = 26.2 bits (55), Expect = 4.1
Identities = 14/29 (48%), Positives = 16/29 (55%)
Frame = -1
Query: 587 DSRNRISNKYETTSCHIFLHGST*CVLCV 501
D RI N Y T+ C LHG T VLC+
Sbjct: 332 DKTIRIWN-YRTSECISILHGHTDSVLCL 359
>SPBP23A10.13 |orc4|orp4|origin recognition complex subunit
Orc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 972
Score = 25.8 bits (54), Expect = 5.5
Identities = 24/112 (21%), Positives = 48/112 (42%), Gaps = 8/112 (7%)
Frame = +3
Query: 129 HKEQAVRLKNLVQQS----DFPHLLIYGPSGAGKKTRVMCLLRELYGSGVERLRQETMNF 296
H ++ +L V+Q+ + ++I GP G+GK V +L E+ +N
Sbjct: 555 HMDEQTKLYQWVRQTIVLGEGNSVIIVGPRGSGKSVLVDDILSRAAQEINEKSYVVRLNG 614
Query: 297 TTPSNKKIEIMTVSSNYHIEVN--PTDVGIHDRVVIMDLVKNVAQT--HQID 440
T ++ K+ + +S IE+ +D + + D + + T H +D
Sbjct: 615 TYQTDDKLALREISRQLSIELESIESDEALKSEMNFSDTLTKLLATLSHPVD 666
>SPAC9E9.04 |||bcap family homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 188
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +3
Query: 534 KYVATCRLILIANSISRVIPAIRSRCLTISVPA 632
K C LIL A+S+ RV+ + L I+ P+
Sbjct: 52 KITIICILILFADSVRRVVRVTKEYDLAIAAPS 84
>SPBC29A3.09c |||AAA family ATPase Gcn20 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 5.5
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 192 IYGPSGAGKKTRVMCLLRELY 254
+ GP+GAGK T + L+ +L+
Sbjct: 549 VVGPNGAGKSTMLKLLIEQLH 569
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 25.8 bits (54), Expect = 5.5
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +3
Query: 192 IYGPSGAGKKTRVMCLLR 245
I GPSG+GK T + LLR
Sbjct: 467 IIGPSGSGKSTFISLLLR 484
>SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 202
Score = 25.8 bits (54), Expect = 5.5
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = +3
Query: 186 LLIYGPSGAGKKTRVMCLLRE 248
++++GPSG GK T + LL++
Sbjct: 21 VVVFGPSGVGKSTLLKRLLKD 41
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 189 LIYGPSGAGKKTRVMCLLREL 251
++ GP+G+GK + + LL EL
Sbjct: 645 IVIGPTGSGKSSLISALLGEL 665
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 25.0 bits (52), Expect = 9.5
Identities = 19/89 (21%), Positives = 37/89 (41%)
Frame = +3
Query: 231 MCLLRELYGSGVERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLV 410
M +LRE YG+ + L + N +I+ S + +N ++ + D + +LV
Sbjct: 535 MAMLRESYGTEINNLLERRNELLDDYNNVQKILNESLEASVHLNTKNLELAD--LNNNLV 592
Query: 411 KNVAQTHQIDSNGQREFKVVILNEVDDLT 497
K + H++ Q + I + T
Sbjct: 593 KQI--QHRVPPENQSNLEHTITTSSKNTT 619
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 186 LLIYGPSGAGKKTRVMCLLREL 251
+ + GP+GAGK T + L EL
Sbjct: 703 IAVIGPNGAGKSTLIKVLTGEL 724
>SPAC3C7.08c |elf1||AAA family ATPase ELf1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1057
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = +3
Query: 192 IYGPSGAGKKTRVMCLLREL 251
I GP+GAGK T + L+ E+
Sbjct: 724 ILGPNGAGKSTLIKVLIGEV 743
>SPAC26H5.02c |||DNA replication ATPase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 504
Score = 25.0 bits (52), Expect = 9.5
Identities = 8/25 (32%), Positives = 17/25 (68%)
Frame = +3
Query: 150 LKNLVQQSDFPHLLIYGPSGAGKKT 224
++NL++Q ++++G +G GK T
Sbjct: 111 IRNLIEQDRCNSMILWGSAGTGKTT 135
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,528,052
Number of Sequences: 5004
Number of extensions: 50057
Number of successful extensions: 169
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 168
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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