Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_G04
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g27740.1 68418.m03327 expressed protein 203 7e-53
At1g21690.1 68414.m02714 replication factor C 37 kDa, putative S... 101 4e-22
At1g77470.1 68414.m09021 replication factor C 36 kDA, putative s... 100 7e-22
At1g21690.2 68414.m02715 replication factor C 37 kDa, putative S... 82 3e-16
At1g63160.1 68414.m07138 replication factor C 40 kDa, putative s... 77 1e-14
At5g66130.1 68418.m08331 cell cycle checkpoint protein-related w... 37 0.010
At3g54540.1 68416.m06035 ABC transporter family protein similar ... 30 1.5
At5g25860.1 68418.m03068 F-box family protein contains F-box dom... 29 2.7
At1g54350.1 68414.m06196 ABC transporter family protein similar ... 29 3.6
At1g71960.1 68414.m08318 ABC transporter family protein similar ... 28 4.7
At5g43690.1 68418.m05340 sulfotransferase family protein similar... 28 6.2
At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A... 28 6.2
At5g38340.1 68418.m04627 disease resistance protein (TIR-NBS-LRR... 27 8.2
At2g41880.1 68415.m05179 guanylate kinase 1 (GK-1) identical to ... 27 8.2
At1g77850.1 68414.m09072 transcriptional factor B3 family protei... 27 8.2
At1g63740.1 68414.m07213 disease resistance protein (TIR-NBS-LRR... 27 8.2
At1g16760.1 68414.m02013 protein kinase family protein contains ... 27 8.2
>At5g27740.1 68418.m03327 expressed protein
Length = 354
Score = 203 bits (496), Expect = 7e-53
Identities = 89/188 (47%), Positives = 136/188 (72%), Gaps = 3/188 (1%)
Frame = +3
Query: 81 LWVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGS 260
LWVDK+RPK L K+ H++ A +LK LV + D PHLL YGPSG+GKKT +M LL+++YG+
Sbjct: 2 LWVDKYRPKSLDKVIVHEDIAQKLKKLVSEQDCPHLLFYGPSGSGKKTLIMALLKQIYGA 61
Query: 261 GVERLRQETMNFTTPSNKK---IEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTH 431
E+++ E + + + +E+ T+SS H+E+ P+D G DR ++ +++K +A+
Sbjct: 62 SAEKVKVENRAWKVDAGSRTIDLELTTLSSTNHVELTPSDAGFQDRYIVQEIIKEMAKNR 121
Query: 432 QIDSNGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRC 611
ID+ G++ +KV++LNEVD L+++AQH+LRRTMEKY ++CRLIL NS S+V AI+SRC
Sbjct: 122 PIDTKGKKGYKVLVLNEVDKLSREAQHSLRRTMEKYSSSCRLILCCNSSSKVTEAIKSRC 181
Query: 612 LTISVPAP 635
L + + AP
Sbjct: 182 LNVRINAP 189
>At1g21690.1 68414.m02714 replication factor C 37 kDa, putative
Similar to SWISS-PROT:P35249 activator 1 37 kDa subunit
(Replication factor C 37 kDa subunit, A1 37 kDa subunit,
RF-C 37 kDa subunit, RFC37) [Homo sapiens]; contains
Pfam domain, PF00004: ATPase, AAA family
Length = 339
Score = 101 bits (242), Expect = 4e-22
Identities = 61/177 (34%), Positives = 91/177 (51%), Gaps = 1/177 (0%)
Frame = +3
Query: 84 WVDKHRPKDLMKLDYHKEQAVR-LKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGS 260
WV+K+RPK + + H+E+ VR L N +Q +D PH+L YGP G GK T + + +L+G
Sbjct: 11 WVEKYRPKQVKDVA-HQEEVVRVLTNTLQTADCPHMLFYGPPGTGKTTTALAIAHQLFG- 68
Query: 261 GVERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQID 440
P K ++ ++++ +N I D + V H+
Sbjct: 69 --------------PELYKSRVLELNASDDRGINVVRTKIKDFAAVA-----VGSNHRQS 109
Query: 441 SNGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRC 611
FK++IL+E D +T+DAQ+ALRRTME Y R I N ISR+I + SRC
Sbjct: 110 GYPCPSFKIIILDEADSMTEDAQNALRRTMETYSKVTRFFFICNYISRIIEPLASRC 166
>At1g77470.1 68414.m09021 replication factor C 36 kDA, putative
similar to SWISS-PROT:P40937 activator 1 36 kDa subunit
(Replication factor C 36 kDa subunit, A1 36 kDa subunit,
RF-C 36 kDa subunit, RFC36) [Homo sapiens]
Length = 369
Score = 100 bits (240), Expect = 7e-22
Identities = 61/180 (33%), Positives = 98/180 (54%)
Frame = +3
Query: 72 KMSLWVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLREL 251
K + WV+K+RP+ L + H++ + L ++ PHLL+YGP G GK + ++ + R+L
Sbjct: 37 KATPWVEKYRPQSLDDVAAHRDIIDTIDRLTNENKLPHLLLYGPPGTGKTSTILAVARKL 96
Query: 252 YGSGVERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTH 431
YG P + N +E+N +D D V+ +++ A T
Sbjct: 97 YG---------------PKYR---------NMILELNASDDRGID--VVRQQIQDFASTQ 130
Query: 432 QIDSNGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRC 611
S G+ K+V+L+E D +TKDAQ ALRR +EKY + R LI N ++++IPA++SRC
Sbjct: 131 SF-SLGKSSVKLVLLDEADAMTKDAQFALRRVIEKYTKSTRFALIGNHVNKIIPALQSRC 189
>At1g21690.2 68414.m02715 replication factor C 37 kDa, putative
Similar to SWISS-PROT:P35249 activator 1 37 kDa subunit
(Replication factor C 37 kDa subunit, A1 37 kDa subunit,
RF-C 37 kDa subunit, RFC37) [Homo sapiens]; contains
Pfam domain, PF00004: ATPase, AAA family
Length = 327
Score = 82.2 bits (194), Expect = 3e-16
Identities = 54/176 (30%), Positives = 82/176 (46%)
Frame = +3
Query: 84 WVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGSG 263
WV+K+RPK + + + +E PH+L YGP G GK T + + +L+G
Sbjct: 11 WVEKYRPKQVKDVAHQEEVC------------PHMLFYGPPGTGKTTTALAIAHQLFG-- 56
Query: 264 VERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQIDS 443
P K ++ ++++ +N I D + V H+
Sbjct: 57 -------------PELYKSRVLELNASDDRGINVVRTKIKDFAAVA-----VGSNHRQSG 98
Query: 444 NGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRC 611
FK++IL+E D +T+DAQ+ALRRTME Y R I N ISR+I + SRC
Sbjct: 99 YPCPSFKIIILDEADSMTEDAQNALRRTMETYSKVTRFFFICNYISRIIEPLASRC 154
>At1g63160.1 68414.m07138 replication factor C 40 kDa, putative
similar to SWISS-PROT:Q9WUK4 activator 1 40 kDa subunit
(Replication factor C 40 kDa subunit, A1 40 kDa subunit,
RF-C 40 kDa subunit, RFC40) [Mus musculus]
Length = 333
Score = 76.6 bits (180), Expect = 1e-14
Identities = 54/179 (30%), Positives = 92/179 (51%)
Frame = +3
Query: 84 WVDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGSG 263
WV+K+RP ++ + +++ RL+ + + + P+L++ GP G GK T ++ L EL G+
Sbjct: 17 WVEKYRPSKVVDIVGNEDAVSRLQVIARDGNMPNLILSGPPGTGKTTSILALAHELLGT- 75
Query: 264 VERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQIDS 443
N K ++ ++++ D GI V+ + +K AQ
Sbjct: 76 ---------------NYKEAVLELNAS-------DDRGID---VVRNKIKMFAQKKVTLP 110
Query: 444 NGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRSRCLTI 620
G+ KVVIL+E D +T AQ ALRRT+E Y + R L N+ +++I I+SRC +
Sbjct: 111 PGRH--KVVILDEADSMTSGAQQALRRTIEIYSNSTRFALACNTSAKIIEPIQSRCALV 167
>At5g66130.1 68418.m08331 cell cycle checkpoint protein-related weak
similarity to cell cycle checkpoint protein RAD17 [Homo
sapiens] GI:4102916
Length = 599
Score = 37.1 bits (82), Expect = 0.010
Identities = 22/58 (37%), Positives = 32/58 (55%), Gaps = 8/58 (13%)
Frame = +3
Query: 81 LWVDKHRPKDLMKLDYHKEQAVRLKNLVQQS-DFPH-------LLIYGPSGAGKKTRV 230
LWVDK+RP+ L +L HK++ +K Q+S DF LL+ G +G GK +
Sbjct: 92 LWVDKYRPRTLEELSVHKKKVDEVKLWFQESLDFLKNGLRNNVLLVTGQAGVGKSATI 149
>At3g54540.1 68416.m06035 ABC transporter family protein similar to
ABC50 GI:10863747 from [Rattus norvegicus]
Length = 723
Score = 29.9 bits (64), Expect = 1.5
Identities = 22/92 (23%), Positives = 39/92 (42%), Gaps = 4/92 (4%)
Frame = +3
Query: 114 MKLDYHKEQAVRLKNLVQQSDF-PHLLIYGPSGAGKKTRVMCLLRELYGSGVERLRQETM 290
+ Y RL N+ D + I GP+GAGK T + L +L + E R + +
Sbjct: 501 VSFSYPNRPDFRLSNVDVGIDMGTRVAIVGPNGAGKSTLLNLLAGDLVPTEGEMRRSQKL 560
Query: 291 NFTTPSNKKIEIMTVSS---NYHIEVNPTDVG 377
S ++++T+ Y + ++P G
Sbjct: 561 RIGRYSQHFVDLLTMGETPVQYLLRLHPDQEG 592
>At5g25860.1 68418.m03068 F-box family protein contains F-box domain
Pfam:PF00646
Length = 448
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/31 (48%), Positives = 21/31 (67%), Gaps = 1/31 (3%)
Frame = +3
Query: 162 VQQSDFPHLLIYGPSGAGKKTR-VMCLLREL 251
V +++ L IYG SG G++ R V CLLRE+
Sbjct: 378 VDRNEVKVLEIYGFSGCGREVRQVKCLLREM 408
>At1g54350.1 68414.m06196 ABC transporter family protein similar to
hypothetical ABC transporter ATP-binding protein
GI:9955395 from [Microcystis aeruginosa]
Length = 706
Score = 28.7 bits (61), Expect = 3.6
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = +3
Query: 174 DFPHLLIYGPSGAGKKTRVMCLLRELYGSG 263
D HLLI GPSG+G KT ++ + L+ SG
Sbjct: 456 DKDHLLIMGPSGSG-KTSLLRAMAGLWRSG 484
>At1g71960.1 68414.m08318 ABC transporter family protein similar to
breast cancer resistance protein GB:AAC97367 from [Homo
sapiens]
Length = 662
Score = 28.3 bits (60), Expect = 4.7
Identities = 14/58 (24%), Positives = 28/58 (48%)
Frame = +3
Query: 93 KHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYGSGV 266
K +P D + + + ++ +F + + GPSG+GK T + + L+GS +
Sbjct: 68 KQKPSDETRSTEERTILSGVTGMISPGEF--MAVLGPSGSGKSTLLNAVAGRLHGSNL 123
>At5g43690.1 68418.m05340 sulfotransferase family protein similar to
steroid sulfotransferase 3 [Brassica napus] GI:3420008,
steroid sulfotransferase 1 [Brassica napus] GI:3420004;
contains Pfam profile PF00685: Sulfotransferase domain
Length = 331
Score = 27.9 bits (59), Expect = 6.2
Identities = 30/117 (25%), Positives = 52/117 (44%), Gaps = 6/117 (5%)
Frame = +3
Query: 75 MSLWVDK-HRPKDLMKLDYHK---EQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLL 242
+S W PK ++ + Y + E V++K L + FP SG+ K +C L
Sbjct: 213 LSYWRGSLENPKHVLFMRYEEMKTEPCVQVKRLAEFLGFPFTKEEEDSGSISKLLELCSL 272
Query: 243 RELYGSGVERLRQETMNFTTPSN-KKIEIMTVSSNYHIEV-NPTDVGIHDRVVIMDL 407
L G V + + MN+ S +K E+ ++ E+ N D+ I +++ DL
Sbjct: 273 GNLSGLEVNKTGKTWMNYDYKSYFRKGEVGDWKNHLTPEMENKIDMIIEEKLKGSDL 329
>At1g25490.1 68414.m03165 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit A (RCN1) identical to
phosphoprotein phosphatase 2A, regulatory subunit A
GI:1262171 from [Arabidopsis thaliana]
Length = 588
Score = 27.9 bits (59), Expect = 6.2
Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +3
Query: 471 ILNEVDDLTKDAQHALR-RTMEKYVATCRLILIANSISRVIPAI 599
I+ DDLTKD Q ++R +E A +L+ + ++R++P I
Sbjct: 203 IMTMFDDLTKDDQDSVRLLAVEGCAALGKLLEPQDCVARILPVI 246
>At5g38340.1 68418.m04627 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1059
Score = 27.5 bits (58), Expect = 8.2
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = +3
Query: 108 DLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRELYG 257
DL+ ++ H E+ L +L ++ + I+GPSG GK T L G
Sbjct: 230 DLIGMEAHMEKMKSLLSL-HSNEVKMIGIWGPSGIGKTTIARVLYNRFSG 278
>At2g41880.1 68415.m05179 guanylate kinase 1 (GK-1) identical to
guanylate kinase (GK-1) [Arabidopsis thaliana]
gi|7861795|gb|AAF70408
Length = 387
Score = 27.5 bits (58), Expect = 8.2
Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +3
Query: 78 SLW---VDKHRPKDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKTRVMCLLRE 248
S+W VD ++ KL KE K + ++ P ++I GPSG GK T + L++E
Sbjct: 103 SIWFLEVDSPYVREQKKL-LRKEVVAWSKGVRGNAEKP-IVISGPSGVGKGTLISMLMKE 160
>At1g77850.1 68414.m09072 transcriptional factor B3 family protein
similar to auxin response factor 10 GI:6165644 from
[Arabidopsis thaliana]; contains Pfam profile PF02362:
B3 DNA binding domain
Length = 585
Score = 27.5 bits (58), Expect = 8.2
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -1
Query: 218 LTGSRGPVNKKVRKVTLLHKI 156
L G+RGP++KKV + L KI
Sbjct: 510 LVGNRGPLSKKVNSIQLFGKI 530
>At1g63740.1 68414.m07213 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 992
Score = 27.5 bits (58), Expect = 8.2
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +3
Query: 105 KDLMKLDYHKEQAVRLKNLVQQSDFPHLLIYGPSGAGKKT 224
+D++ ++ H E+ L +L + + IYGP+G GK T
Sbjct: 142 EDMVGVETHLEKIQSLLHLDNDDEAMIVGIYGPAGIGKTT 181
>At1g16760.1 68414.m02013 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 758
Score = 27.5 bits (58), Expect = 8.2
Identities = 22/116 (18%), Positives = 46/116 (39%)
Frame = +3
Query: 258 SGVERLRQETMNFTTPSNKKIEIMTVSSNYHIEVNPTDVGIHDRVVIMDLVKNVAQTHQI 437
SG + + + + P + + M+ SS I + + D + + +
Sbjct: 254 SGRPSVDRSSFTYDLPESARTSRMSTSSEQSIGSHRLGIKFTD----LSYLNGSSSVSDE 309
Query: 438 DSNGQREFKVVILNEVDDLTKDAQHALRRTMEKYVATCRLILIANSISRVIPAIRS 605
F LN+V+ K + L++TM+ Y + CR L A + + + +R+
Sbjct: 310 SGRTSCSFSSQSLNDVEAQMKRLRLELKQTMDMYSSACREALTARNEATELQKLRT 365
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,790,547
Number of Sequences: 28952
Number of extensions: 249092
Number of successful extensions: 852
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 813
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 850
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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