Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_G02
(551 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_16632| Best HMM Match : HSP20 (HMM E-Value=1e-12) 70 1e-12
SB_21425| Best HMM Match : HSP20 (HMM E-Value=1.9e-26) 66 2e-11
SB_49292| Best HMM Match : HSP20 (HMM E-Value=1.9e-29) 62 4e-10
SB_38963| Best HMM Match : HSP20 (HMM E-Value=7.3e-26) 59 3e-09
SB_37349| Best HMM Match : HSP20 (HMM E-Value=7.3e-26) 59 3e-09
SB_21424| Best HMM Match : HSP20 (HMM E-Value=0) 57 8e-09
SB_3291| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 8e-09
SB_58906| Best HMM Match : HSP20 (HMM E-Value=3e-26) 56 1e-08
SB_58907| Best HMM Match : HSP20 (HMM E-Value=4.2e-05) 39 0.002
SB_59044| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.83
SB_16236| Best HMM Match : No HMM Matches (HMM E-Value=.) 27 1.3
SB_23220| Best HMM Match : RVT_1 (HMM E-Value=3.8e-32) 27 7.7
>SB_16632| Best HMM Match : HSP20 (HMM E-Value=1e-12)
Length = 210
Score = 70.1 bits (164), Expect = 1e-12
Identities = 34/85 (40%), Positives = 56/85 (65%), Gaps = 1/85 (1%)
Frame = +1
Query: 274 VGSSIKVDKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRY 450
+ ++ +++ DK ++ LDV+++ PE IS+K G I ++GKH + + EHGY + +F R Y
Sbjct: 29 LAANTEMEGDKVEIATLDVKNYRPEEISLKVEHGRIKIDGKH-KSEGEHGYETSEFHRSY 87
Query: 451 ALPEGAAPETVESRLSSDGVLTITA 525
LP+G TV SR++ DG+L I A
Sbjct: 88 NLPDGVDVSTVSSRITGDGLLHIEA 112
Score = 27.9 bits (59), Expect(2) = 0.18
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = +1
Query: 253 LAAAARDVGSSIKVDKDKFQVNLDVQHFAPE 345
+ A+ G K+D +F V LDV F+P+
Sbjct: 125 VGASTGSAGDIAKIDDKRFTVTLDVSSFSPD 155
Score = 23.8 bits (49), Expect(2) = 0.18
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +1
Query: 442 RRYALPEGAAPETVESRLSSDGVLTITA 525
R + LP+ +++ SRL DG L I A
Sbjct: 161 RHFVLPKDVDMDSLVSRLGKDGKLYIEA 188
>SB_21425| Best HMM Match : HSP20 (HMM E-Value=1.9e-26)
Length = 185
Score = 66.1 bits (154), Expect = 2e-11
Identities = 35/86 (40%), Positives = 51/86 (59%), Gaps = 1/86 (1%)
Frame = +1
Query: 292 VDKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGA 468
+D DKFQ+ LDV+ F PE I+ K +G I V G + E G+ S++F R Y LPEG
Sbjct: 1 MDADKFQIATLDVREFKPEEITCKVENGKIKVSGLQRH-ESEEGFDSKEFRRCYNLPEGV 59
Query: 469 APETVESRLSSDGVLTITAPXKVPDA 546
++ +R++ DG+L + A K P A
Sbjct: 60 DESSISTRIAEDGMLHVEALKKSPPA 85
Score = 50.0 bits (114), Expect = 1e-06
Identities = 30/78 (38%), Positives = 40/78 (51%), Gaps = 1/78 (1%)
Frame = +1
Query: 295 DKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYIS-RQFVRRYALPEGAA 471
D KF + LDV F PE + VK + V + + EHG+ + RQF R + LP
Sbjct: 96 DDTKFTLALDVSDFKPEEVDVKVYGHELSVRAR--QECEEHGFFTARQFNRHFVLPREVD 153
Query: 472 PETVESRLSSDGVLTITA 525
+T+ RL DGVL I A
Sbjct: 154 MDTLVPRLGKDGVLYIEA 171
>SB_49292| Best HMM Match : HSP20 (HMM E-Value=1.9e-29)
Length = 189
Score = 61.7 bits (143), Expect = 4e-10
Identities = 31/74 (41%), Positives = 45/74 (60%)
Frame = +1
Query: 319 LDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAAPETVESRLS 498
LDV+ + PE IS K +G + V+G+H + E G+ ++F R + LPEG PE V SR+S
Sbjct: 12 LDVKEYRPEEISFKVENGVVKVQGRHVN-EGEFGFELKEFRRTFTLPEGIDPENVTSRIS 70
Query: 499 SDGVLTITAPXKVP 540
+ G L I A +P
Sbjct: 71 NHGHLHIEARKALP 84
Score = 48.8 bits (111), Expect = 3e-06
Identities = 27/87 (31%), Positives = 43/87 (49%)
Frame = +1
Query: 289 KVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGA 468
K +DKF + +DV F PE I V+ ++V H E + E Y + F R++ LP
Sbjct: 92 KAKEDKFSMAIDVAGFPPESIKVQVLGNELLVNANH-EVEHEGHYHAMHFNRQFVLPREV 150
Query: 469 APETVESRLSSDGVLTITAPXKVPDAV 549
+++ +RL +G L A V A+
Sbjct: 151 DMDSLTTRLDKEGKLHFEAKKSVAPAL 177
>SB_38963| Best HMM Match : HSP20 (HMM E-Value=7.3e-26)
Length = 190
Score = 58.8 bits (136), Expect = 3e-09
Identities = 29/83 (34%), Positives = 51/83 (61%), Gaps = 1/83 (1%)
Frame = +1
Query: 295 DKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAA 471
+++K ++ LDV+ + PE IS K +G++ V+G+H + G+ ++F R +ALPEG
Sbjct: 3 NENKLEIAKLDVREYRPEEISFKVENGFVKVQGRHVN-EGPFGFELKEFRRTFALPEGVE 61
Query: 472 PETVESRLSSDGVLTITAPXKVP 540
V++R+S+ G L I A +P
Sbjct: 62 ASNVKTRISNHGQLHIEAMKALP 84
Score = 44.4 bits (100), Expect = 6e-05
Identities = 25/85 (29%), Positives = 40/85 (47%)
Frame = +1
Query: 271 DVGSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRY 450
D G DKF + +DV F P+ I V+ ++V H E + E Y + F R++
Sbjct: 87 DGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANH-EVEHEGHYHAMHFNRQF 145
Query: 451 ALPEGAAPETVESRLSSDGVLTITA 525
LP +++ +RL +G L A
Sbjct: 146 VLPREVDMDSLTTRLDKEGKLHFEA 170
>SB_37349| Best HMM Match : HSP20 (HMM E-Value=7.3e-26)
Length = 190
Score = 58.8 bits (136), Expect = 3e-09
Identities = 29/83 (34%), Positives = 51/83 (61%), Gaps = 1/83 (1%)
Frame = +1
Query: 295 DKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAA 471
+++K ++ LDV+ + PE IS K +G++ V+G+H + G+ ++F R +ALPEG
Sbjct: 3 NENKLEIAKLDVREYRPEEISFKVENGFVKVQGRHVN-EGPFGFELKEFRRTFALPEGVE 61
Query: 472 PETVESRLSSDGVLTITAPXKVP 540
V++R+S+ G L I A +P
Sbjct: 62 ASNVKTRISNHGQLHIEAMKALP 84
Score = 44.4 bits (100), Expect = 6e-05
Identities = 25/85 (29%), Positives = 40/85 (47%)
Frame = +1
Query: 271 DVGSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRY 450
D G DKF + +DV F P+ I V+ ++V H E + E Y + F R++
Sbjct: 87 DGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANH-EVEHEGHYHAMHFNRQF 145
Query: 451 ALPEGAAPETVESRLSSDGVLTITA 525
LP +++ +RL +G L A
Sbjct: 146 VLPREVDMDSLTTRLDKEGKLHFEA 170
>SB_21424| Best HMM Match : HSP20 (HMM E-Value=0)
Length = 424
Score = 57.2 bits (132), Expect = 8e-09
Identities = 28/83 (33%), Positives = 50/83 (60%), Gaps = 1/83 (1%)
Frame = +1
Query: 295 DKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAA 471
+++K ++ LDV+ + PE IS K +G++ V+G+H + G+ ++F R + LPEG
Sbjct: 3 NENKLEIAKLDVREYRPEEISFKVENGFVKVQGRHVN-EGPFGFELKEFRRTFTLPEGVE 61
Query: 472 PETVESRLSSDGVLTITAPXKVP 540
V++R+S+ G L I A +P
Sbjct: 62 ASNVKTRISNHGQLHIEAMKALP 84
Score = 56.8 bits (131), Expect = 1e-08
Identities = 28/81 (34%), Positives = 48/81 (59%), Gaps = 1/81 (1%)
Frame = +1
Query: 301 DKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAAPE 477
D+ ++ LDV+ + PE IS K +G++ V+G+H + G+ ++F R + LPEG
Sbjct: 239 DELEIAKLDVREYRPEEISFKVENGFVKVQGRHVN-EGPFGFELKEFRRTFTLPEGVEAS 297
Query: 478 TVESRLSSDGVLTITAPXKVP 540
V++R+S+ G L I A +P
Sbjct: 298 NVKTRISNHGQLHIEAMKALP 318
Score = 44.4 bits (100), Expect = 6e-05
Identities = 25/85 (29%), Positives = 40/85 (47%)
Frame = +1
Query: 271 DVGSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRY 450
D G DKF + +DV F P+ I V+ ++V H E + E Y + F R++
Sbjct: 87 DGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANH-EVEHEGHYHAMHFNRQF 145
Query: 451 ALPEGAAPETVESRLSSDGVLTITA 525
LP +++ +RL +G L A
Sbjct: 146 VLPREVDMDSLTTRLDKEGKLHFEA 170
Score = 44.4 bits (100), Expect = 6e-05
Identities = 25/85 (29%), Positives = 40/85 (47%)
Frame = +1
Query: 271 DVGSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRY 450
D G DKF + +DV F P+ I V+ ++V H E + E Y + F R++
Sbjct: 321 DGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANH-EVEHEGHYHAMHFNRQF 379
Query: 451 ALPEGAAPETVESRLSSDGVLTITA 525
LP +++ +RL +G L A
Sbjct: 380 VLPREVDMDSLTTRLDKEGKLHFEA 404
>SB_3291| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 760
Score = 57.2 bits (132), Expect = 8e-09
Identities = 28/83 (33%), Positives = 50/83 (60%), Gaps = 1/83 (1%)
Frame = +1
Query: 295 DKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAA 471
+++K ++ LDV+ + PE IS K +G++ V+G+H + G+ ++F R + LPEG
Sbjct: 3 NENKLEIAKLDVREYRPEEISFKVENGFVKVQGRHVN-EGPFGFELKEFRRTFTLPEGVE 61
Query: 472 PETVESRLSSDGVLTITAPXKVP 540
V++R+S+ G L I A +P
Sbjct: 62 ASNVKTRISNHGQLHIEAMKALP 84
Score = 44.4 bits (100), Expect = 6e-05
Identities = 25/85 (29%), Positives = 40/85 (47%)
Frame = +1
Query: 271 DVGSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRY 450
D G DKF + +DV F P+ I V+ ++V H E + E Y + F R++
Sbjct: 87 DGGEEESKGDDKFSMAMDVTGFPPDSIKVQVLGNELLVSANH-EVEHEGHYHAMHFNRQF 145
Query: 451 ALPEGAAPETVESRLSSDGVLTITA 525
LP +++ +RL +G L A
Sbjct: 146 VLPREVDMDSLTTRLDKEGKLHFEA 170
>SB_58906| Best HMM Match : HSP20 (HMM E-Value=3e-26)
Length = 695
Score = 56.4 bits (130), Expect = 1e-08
Identities = 28/84 (33%), Positives = 50/84 (59%), Gaps = 1/84 (1%)
Frame = +1
Query: 295 DKDKFQV-NLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYALPEGAA 471
+++K ++ LDV+ + PE IS K +G + V+G+H + G+ ++F R + LPEG
Sbjct: 4 NENKLEIAKLDVREYRPEEISFKVENGVVKVQGRHVN-EGPFGFELKEFRRTFTLPEGVE 62
Query: 472 PETVESRLSSDGVLTITAPXKVPD 543
V++R+S+ G L I A +P+
Sbjct: 63 ASNVKTRISNHGQLHIEAMKALPE 86
Score = 45.6 bits (103), Expect = 3e-05
Identities = 25/83 (30%), Positives = 42/83 (50%)
Frame = +1
Query: 277 GSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYISRQFVRRYAL 456
G+ + DKF + +DV+ F PE I V+ ++V H E + E Y + F R++ L
Sbjct: 89 GAKKESKDDKFSMAMDVKGFPPEAIKVQVLGNELLVSANH-EVEHEGHYHAMHFNRQFIL 147
Query: 457 PEGAAPETVESRLSSDGVLTITA 525
P +++ +RL +G L A
Sbjct: 148 PREVDMDSLTTRLDKEGKLHFEA 170
>SB_58907| Best HMM Match : HSP20 (HMM E-Value=4.2e-05)
Length = 259
Score = 39.1 bits (87), Expect = 0.002
Identities = 23/68 (33%), Positives = 35/68 (51%), Gaps = 1/68 (1%)
Frame = +1
Query: 262 AARDVGSSIKVDKDKFQVNLDVQHFAPEXISVKTADGYIVVEGKHXEXKXEHGYI-SRQF 438
A ++ S+ D KF + LDV F PE + VK + V + + EHG+ +RQF
Sbjct: 13 ATKENQSAATKDDTKFTLALDVSDFKPEEVDVKVYGHELSVRAR--QECEEHGFFTARQF 70
Query: 439 VRRYALPE 462
R + LP+
Sbjct: 71 NRHFVLPQ 78
>SB_59044| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 96
Score = 30.7 bits (66), Expect = 0.83
Identities = 17/58 (29%), Positives = 28/58 (48%)
Frame = -3
Query: 210 GSDTKKIIWC*GKAKMLVQQTARASRAKIIEEIW*QRHSCFLLKITPGKSLGYNVCMF 37
G++ + WC + LV TA + A I + W Q + FL+ I+ G G N+ +
Sbjct: 30 GANNSQYYWCQKQPVFLVPITASITGASIRQYFWCQYQAVFLVPIS-GSITGANISQY 86
>SB_16236| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2317
Score = 27.1 bits (57), Expect(2) = 1.3
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = -1
Query: 335 KCCTSKFTWN 306
KCCTS +TWN
Sbjct: 1829 KCCTSNYTWN 1838
Score = 21.4 bits (43), Expect(2) = 1.3
Identities = 5/10 (50%), Positives = 8/10 (80%)
Frame = -1
Query: 332 CCTSKFTWNL 303
CCT ++ WN+
Sbjct: 1860 CCTHEYGWNI 1869
>SB_23220| Best HMM Match : RVT_1 (HMM E-Value=3.8e-32)
Length = 1597
Score = 27.5 bits (58), Expect = 7.7
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 5/37 (13%)
Frame = -1
Query: 212 PAATLRRSSGVKARPK-----CWSNRRRGRLEPKSSK 117
PA+TLRRS+ +K P CW+ PK+SK
Sbjct: 1313 PASTLRRSTRLKTTPTHLKDYCWTKHVARASVPKNSK 1349
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,848,228
Number of Sequences: 59808
Number of extensions: 327898
Number of successful extensions: 882
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 877
length of database: 16,821,457
effective HSP length: 78
effective length of database: 12,156,433
effective search space used: 1276425465
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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