Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P03_F_G02
         (551 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote...    35   0.042
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS...    34   0.073
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS...    33   0.096
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote...    32   0.22 
At4g14830.1 68417.m02280 expressed protein                             29   2.1  
At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl methyl...    28   3.6  
At1g22080.1 68414.m02761 hypothetical protein                          28   3.6  
At1g66880.1 68414.m07601 serine/threonine protein kinase family ...    28   4.8  
At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase, puta...    27   6.3  

>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
           (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
           heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
           (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
           17 (19), 7995 (1989))
          Length = 157

 Score = 34.7 bits (76), Expect = 0.042
 Identities = 25/82 (30%), Positives = 41/82 (50%), Gaps = 8/82 (9%)
 Frame = +1

Query: 322 DVQHFAPEXISVKTADGYIV-VEGKHXEXKXEHG-------YISRQFVRRYALPEGAAPE 477
           D+     E + V+  DG I+ + G+      E           S +F RR+ LPE A  E
Sbjct: 66  DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125

Query: 478 TVESRLSSDGVLTITAPXKVPD 543
            +++ +  +GVL++T P KVP+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPE 145


>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
           (HSP17.4-CI) identical to 17.4 kDa class I heat shock
           protein SP:P19036 from [Arabidopsis thaliana]
          Length = 156

 Score = 33.9 bits (74), Expect = 0.073
 Identities = 24/77 (31%), Positives = 38/77 (49%), Gaps = 8/77 (10%)
 Frame = +1

Query: 322 DVQHFAPEXISVKTADGYIV-VEGKHXEXKXEHGYI-------SRQFVRRYALPEGAAPE 477
           DV     E + V+  DG I+ + G+      E           S +F+RR+ LPE A  E
Sbjct: 65  DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124

Query: 478 TVESRLSSDGVLTITAP 528
            V++ +  +GVL++T P
Sbjct: 125 EVKASM-ENGVLSVTVP 140


>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
           (HSP18.1-CI) identical to 18.2 kDa class I heat shock
           protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
           contains Pfam profile: PF00011 Hsp20/alpha crystallin
           family
          Length = 161

 Score = 33.5 bits (73), Expect = 0.096
 Identities = 33/110 (30%), Positives = 58/110 (52%), Gaps = 14/110 (12%)
 Frame = +1

Query: 256 AAAARDVGS--SIKVD-KDKFQVNL---DVQHFAPEXISVKTADGYIV-VEG---KHXEX 405
           A+ ARDV +  + +VD K+  + ++   D+     E + V+  D  ++ + G   K  E 
Sbjct: 40  ASTARDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENEE 99

Query: 406 KXEHGY----ISRQFVRRYALPEGAAPETVESRLSSDGVLTITAPXKVPD 543
           K +  +     S +F+RR+ LPE A  E V++ +  +GVLT+  P K P+
Sbjct: 100 KNDKWHRVERASGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPE 147


>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
           (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
           crystallin family; identified in Scharf, K-D., et al,
           Cell Stress & Chaperones (2001) 6: 225-237.
          Length = 153

 Score = 32.3 bits (70), Expect = 0.22
 Identities = 15/34 (44%), Positives = 22/34 (64%)
 Frame = +1

Query: 427 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAP 528
           S QF RR+ LPE    + V++ +  +GVLT+T P
Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVP 137


>At4g14830.1 68417.m02280 expressed protein
          Length = 152

 Score = 29.1 bits (62), Expect = 2.1
 Identities = 15/35 (42%), Positives = 18/35 (51%)
 Frame = +1

Query: 439 VRRYALPEGAAPETVESRLSSDGVLTITAPXKVPD 543
           V R+ LPE   PE V      DG L +T P K+ D
Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVP-KIED 142


>At1g66720.1 68414.m07584 S-adenosyl-L-methionine:carboxyl
           methyltransferase family protein similar to
           defense-related protein cjs1 [Brassica
           carinata][GI:14009292][Mol Plant Pathol (2001)
           2(3):159-169]
          Length = 352

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 10/31 (32%), Positives = 19/31 (61%)
 Frame = +2

Query: 437 SSDVTRCLKARRLRLWNRDCHQTGYSPSLRR 529
           +S + + +  R   LWNRD H TG++  +++
Sbjct: 151 TSKIPKGISDRNSPLWNRDMHCTGFNNKVKK 181


>At1g22080.1 68414.m02761 hypothetical protein
          Length = 475

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 15/43 (34%), Positives = 21/43 (48%)
 Frame = -1

Query: 227 LFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKYGSND 99
           L  + P A L+R    +  PK    RRR R+   SSK + + D
Sbjct: 305 LMDEAPRANLKRKQEQQDEPKRSKKRRRQRVARSSSKLHSNLD 347


>At1g66880.1 68414.m07601 serine/threonine protein kinase family
            protein contains protein kinase domain, Pfam:PF00069;
            contains serine/threonine protein kinase domain,
            INTERPRO:IPR002290
          Length = 1296

 Score = 27.9 bits (59), Expect = 4.8
 Identities = 13/41 (31%), Positives = 18/41 (43%)
 Frame = -1

Query: 233  YSLFSKGPAATLRRSSGVKARPKCWSNRRRGRLEPKSSKKY 111
            Y   S G  A     +  +ARP CWS R    +E  S+  +
Sbjct: 1041 YEYISNGTLAEHLHGNRAEARPLCWSTRLNIAIETASALSF 1081


>At4g22590.1 68417.m03259 trehalose-6-phosphate phosphatase,
           putative similar to trehalose-6-phosphate phosphatase
           (AtTPPA) GI:2944178; contains Pfam profile PF02358:
           Trehalose-phosphatase
          Length = 377

 Score = 27.5 bits (58), Expect = 6.3
 Identities = 16/57 (28%), Positives = 28/57 (49%)
 Frame = +1

Query: 133 SRRPRRLLDQHFGLALTPDDLLSVAAGPLLNREYYRPWRHLAAAARDVGSSIKVDKD 303
           S  PR+ L + F + + P+D  S  A  L        + H+AA A++   ++ +D D
Sbjct: 59  SSPPRKRLVKDFNIEIAPEDDFSQRAWMLKYPSAITSFAHIAAQAKNKKIAVFLDYD 115


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,482,401
Number of Sequences: 28952
Number of extensions: 220083
Number of successful extensions: 539
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 526
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 539
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1043173136
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)