Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_F06
(643 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 31 0.65
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 31 0.65
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 31 0.86
At3g54310.1 68416.m06002 hypothetical protein predicted protein,... 29 2.6
At3g42800.1 68416.m04480 expressed protein hypothetical proteins... 29 3.5
At4g24805.1 68417.m03553 expressed protein 28 6.1
At3g46360.1 68416.m05021 hypothetical protein 28 6.1
At2g37520.1 68415.m04601 PHD finger family protein contains Pfam... 28 6.1
At2g34150.1 68415.m04180 expressed protein 28 6.1
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 31.1 bits (67), Expect = 0.65
Identities = 23/111 (20%), Positives = 47/111 (42%)
Frame = +3
Query: 267 SLAALGXGGDPKSQKELFEVLNLPAEACKRERYYQIANSLATPGTDVHLTTTRALILDNN 446
S+ LG G P+ ++++ + + + Y+I S A PG+ V + A I+
Sbjct: 520 SVRVLGEGYSPEDEEDM--TIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTA 577
Query: 447 LIINPDWSKFVHTAKLLHIVRAPIKKHLTDHENPIXXREITQDEESFGNSF 599
+ N + + V+ + L P+ K T+ NP ++ + S+
Sbjct: 578 TLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSY 628
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 31.1 bits (67), Expect = 0.65
Identities = 23/111 (20%), Positives = 47/111 (42%)
Frame = +3
Query: 267 SLAALGXGGDPKSQKELFEVLNLPAEACKRERYYQIANSLATPGTDVHLTTTRALILDNN 446
S+ LG G P+ ++++ + + + Y+I S A PG+ V + A I+
Sbjct: 520 SVRVLGEGYSPEDEEDM--TIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTA 577
Query: 447 LIINPDWSKFVHTAKLLHIVRAPIKKHLTDHENPIXXREITQDEESFGNSF 599
+ N + + V+ + L P+ K T+ NP ++ + S+
Sbjct: 578 TLCNASYDEDVYIFRALQFNTLPVVKTATEPLNPSELPKMVEGLRKISKSY 628
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 30.7 bits (66), Expect = 0.86
Identities = 23/111 (20%), Positives = 47/111 (42%)
Frame = +3
Query: 267 SLAALGXGGDPKSQKELFEVLNLPAEACKRERYYQIANSLATPGTDVHLTTTRALILDNN 446
S+ LG G P+ ++++ + + + Y+I S A PG+ V + A I+
Sbjct: 506 SVRVLGEGYSPEDEEDM--TIKEVTKLWIYQARYRIPVSSAPPGSWVLIEGVDASIMKTA 563
Query: 447 LIINPDWSKFVHTAKLLHIVRAPIKKHLTDHENPIXXREITQDEESFGNSF 599
+ N + + V+ + L P+ K T+ NP ++ + S+
Sbjct: 564 TLCNASYDEDVYIFRALKFNTLPVVKTATEPLNPSELPKMVEGLRKISKSY 614
>At3g54310.1 68416.m06002 hypothetical protein predicted protein,
Arabidopsis thaliana
Length = 358
Score = 29.1 bits (62), Expect = 2.6
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -2
Query: 162 DSDLHCRCLEELNCQRHVNL 103
D + C+C+ ELN RH+NL
Sbjct: 195 DVTISCKCMAELNALRHLNL 214
>At3g42800.1 68416.m04480 expressed protein hypothetical proteins -
Arabidopsis thaliana; expression supported by MPSS
Length = 341
Score = 28.7 bits (61), Expect = 3.5
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = +2
Query: 443 QSNNKPGLVQVCSHSKTTAYSASSNKETSNRPRKSD*XXRNNAR 574
QSN K V++CS KTT +SS+K +R R NN +
Sbjct: 172 QSNAKK--VKLCSVGKTTDVKSSSSKSCFSRTRNKTDNNNNNCK 213
>At4g24805.1 68417.m03553 expressed protein
Length = 247
Score = 27.9 bits (59), Expect = 6.1
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = +1
Query: 289 EETRSRKRNYSKFSIYLPKHVNAKGITKLRIVWPRRE 399
E R R YS + Y+ +N KLR VW R+
Sbjct: 49 EGIRIRHHGYSSYEAYIKHQLNKTQNPKLRKVWTTRD 85
>At3g46360.1 68416.m05021 hypothetical protein
Length = 197
Score = 27.9 bits (59), Expect = 6.1
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -1
Query: 586 KDSSSCVISLXSIGFSWSVRCFFIGARTICS 494
+DSS V+SL +GFS ++ F G + + S
Sbjct: 75 RDSSDIVVSLLCVGFSEALGFLFYGGQCVSS 105
>At2g37520.1 68415.m04601 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 854
Score = 27.9 bits (59), Expect = 6.1
Identities = 11/33 (33%), Positives = 23/33 (69%), Gaps = 2/33 (6%)
Frame = +1
Query: 319 SKFSIYLPKHVNAKGITKL--RIVWPRRELTSI 411
S+F + +PKH+++ GITK+ ++ P++E +
Sbjct: 115 SRFVLEIPKHLSSTGITKITFKLSKPKKEFDDL 147
>At2g34150.1 68415.m04180 expressed protein
Length = 700
Score = 27.9 bits (59), Expect = 6.1
Identities = 19/66 (28%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = +3
Query: 435 LDNNLIINPDWSKFVHTAKLLHIVRAPI-KKHLTDHENPIXXREITQDEESFGNSFLIDT 611
+ NN + D +K T++L H+ +P+ K H++P EI+ S+ N F
Sbjct: 280 ISNNFSV--DETKCAATSEL-HLSSSPVYKSDELIHQDPWAASEISSGTHSYSNGFSNPL 336
Query: 612 LDYSGL 629
D SG+
Sbjct: 337 YDISGI 342
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,223,886
Number of Sequences: 28952
Number of extensions: 285754
Number of successful extensions: 790
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 790
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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