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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P03_F_E21
         (640 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M...    31   0.11 
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    28   1.3  
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    25   7.0  
SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces...    25   9.2  
SPAC1039.08 |||serine acetyltransferase |Schizosaccharomyces pom...    25   9.2  

>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 685

 Score = 31.5 bits (68), Expect = 0.11
 Identities = 14/35 (40%), Positives = 23/35 (65%), Gaps = 5/35 (14%)
 Frame = +2

Query: 452 RWLHEAYGLGRFGYAQP-----ERFHYSISGXQER 541
           R++HEA+G+  FG + P     E+FH++ SG  +R
Sbjct: 629 RYVHEAFGMHTFGDSGPAPKLYEKFHFTTSGVAQR 663


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1811

 Score = 27.9 bits (59), Expect = 1.3
 Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 1/50 (2%)
 Frame = +2

Query: 269  HELHR*ELLQVRSDRSHHCRHYCFHCRVLRLLWRCQREPLH-DNNVFSIP 415
            HE+ R     V   + HH +     C +  L+  C  E  H DN + +IP
Sbjct: 1594 HEISRFNSQSVFKSKKHHLKSIVVKCTLQLLMLNCLWELFHSDNMLTNIP 1643



 Score = 26.2 bits (55), Expect = 4.0
 Identities = 10/35 (28%), Positives = 17/35 (48%)
 Frame = -3

Query: 515  NGSVQVAHNRIFQVRMLHVTSDAHSQFSHEYDQEE 411
            +GS+ + H++ FQ    H T  +  +   EY   E
Sbjct: 1111 SGSISLKHSKSFQSASTHSTKSSSVEIVREYSSRE 1145


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -1

Query: 370 APQQPKNATMKTIMPTMMRT 311
           AP QPK +   +I+PT+ RT
Sbjct: 810 APVQPKTSQASSILPTVPRT 829


>SPAC32A11.01 |mug8||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 720

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = -2

Query: 75  NKMSSQPAANKGXSRATVSSASNKF 1
           N +++ P  ++G +  TVSSAS+ F
Sbjct: 568 NMLNTLPPTSQGATSTTVSSASSNF 592


>SPAC1039.08 |||serine acetyltransferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/26 (34%), Positives = 14/26 (53%)
 Frame = +2

Query: 401 VFSIPLDHIRG*TGCGHRWLHEAYGL 478
           V ++PLDH+ G +     W +   GL
Sbjct: 55  VLALPLDHVTGSSESMENWFYSILGL 80


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,227,295
Number of Sequences: 5004
Number of extensions: 38921
Number of successful extensions: 95
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 91
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 95
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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