Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_E11
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.12 |dcp2||mRNA decapping complex subunit Dcp2|Schizosac... 35 0.009
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 33 0.027
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 30 0.25
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 28 1.0
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 27 2.4
SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 26 4.1
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 26 5.5
SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein Tea4|Schi... 26 5.5
SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces pombe... 25 7.2
SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual 25 7.2
SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 25 7.2
SPCC338.07c |||NatA N-acetyltransferase complex subunit |Schizos... 25 7.2
SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit Rpt6|S... 25 9.5
>SPAC19A8.12 |dcp2||mRNA decapping complex subunit
Dcp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 741
Score = 35.1 bits (77), Expect = 0.009
Identities = 25/109 (22%), Positives = 50/109 (45%)
Frame = +1
Query: 253 LKHKRSTPTNNYERNEQATLQWAKTSMKELXMEIREIGESVNNSALLRQLHIIRNELKQA 432
LK TP N+ + Q + A + +KEL E++ +S + L + L I RN+
Sbjct: 589 LKSGLPTPANDLQNKSQNNERKASSQVKEL--EVKNYSKSTD--LLKKTLRIPRNDEPLE 644
Query: 433 LTETSDLAQLARTQEARLHKMDNEVGRLKYEGQQMRSTVAELRTHVQQA 579
DL +++ Q++ + NE+ + K + ++ + + HV +
Sbjct: 645 AANQFDLLKVSPQQKSEVPPKRNELSQSKLKNRKKKENSETNKNHVDMS 693
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 33.5 bits (73), Expect = 0.027
Identities = 27/151 (17%), Positives = 71/151 (47%), Gaps = 5/151 (3%)
Frame = +1
Query: 139 IKAELRFKEELQAVAEQVEIMKTLHLTDITRLKSEIEELKHKRSTPTNNYERNEQATLQW 318
+ E+R KE++ E + I L++ + +S + + K++ ++ R + ++
Sbjct: 1402 LNQEIRLKEDVLKEKESLIISLEESLSNQRQKESSLLDAKNELEHMLDDTSRKNSSLMEK 1461
Query: 319 AKTSMKELXMEIREIGESVNNSALLRQLHIIRNELKQAL-TETSDLAQLARTQEARLHKM 495
++ L + E+ +V L++LH L + + ++ + + + E+ + ++
Sbjct: 1462 IESINSSLDDKSFELASAVEKLGALQKLHSESLSLMENIKSQLQEAKEKIQVDESTIQEL 1521
Query: 496 DNEVGRLK--YEGQ--QMRSTVAELRTHVQQ 576
D+E+ K YEG+ S + +L +++Q
Sbjct: 1522 DHEITASKNNYEGKLNDKDSIIRDLSENIEQ 1552
>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1115
Score = 30.3 bits (65), Expect = 0.25
Identities = 28/140 (20%), Positives = 62/140 (44%)
Frame = +1
Query: 136 VIKAELRFKEELQAVAEQVEIMKTLHLTDITRLKSEIEELKHKRSTPTNNYERNEQATLQ 315
+++ E +++E++ + E VE +IT+ + +E+ K S +++YE
Sbjct: 523 LMRMEQQWREDVDQLQEYVE--------EITQELQDTKEVLSKSSKESDDYEEVVGKLRT 574
Query: 316 WAKTSMKELXMEIREIGESVNNSALLRQLHIIRNELKQALTETSDLAQLARTQEARLHKM 495
A+ +++ IRE ES+ S ++ + +E+ Q +D + RL +
Sbjct: 575 EAEREIEKFEKTIRENEESI--SLFKEEVEKLTDEITQLSERYNDKCHEFDELQKRLQTL 632
Query: 496 DNEVGRLKYEGQQMRSTVAE 555
+ E + K + S + E
Sbjct: 633 EEENNKAKEDSTSKTSNLLE 652
Score = 27.5 bits (58), Expect = 1.8
Identities = 31/138 (22%), Positives = 68/138 (49%), Gaps = 1/138 (0%)
Frame = +1
Query: 154 RFKEELQAVAEQVEIMKTLHLTDITRLKSEIEELKHKRSTPTNNYERNEQATLQWAKTSM 333
R +EL ++ +++ + + L+ + LKSEIEE ++ T N++ + A ++ +
Sbjct: 730 RHNDELDSLNKKLREFEGI-LSSNSSLKSEIEERNNQYVTLRENFDSLQNAIMETFDKQV 788
Query: 334 KELXMEIREIGESVNNSALLRQLHIIRNELKQALTETSDLAQLARTQEARLHKMDNEVGR 513
SVN+ L++Q+ +++E K+ + T L + E L + N +
Sbjct: 789 THC---------SVNH--LVQQIRKLKDENKKDQSGTDKLMKKIYHCEQSLKEKTNSLET 837
Query: 514 LKYEGQQMRSTV-AELRT 564
L E +++++ + AE R+
Sbjct: 838 LVSEKKELKNLLDAERRS 855
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 28.3 bits (60), Expect = 1.0
Identities = 27/96 (28%), Positives = 43/96 (44%)
Frame = +1
Query: 154 RFKEELQAVAEQVEIMKTLHLTDITRLKSEIEELKHKRSTPTNNYERNEQATLQWAKTSM 333
R K EL + +E + +I LKSE+E +K K S N Q+ ++ +
Sbjct: 1321 RLKNELVSKENLIEELNQ----EIGHLKSELETVKSK-SEDLENERAQNQSKIEQLELKN 1375
Query: 334 KELXMEIREIGESVNNSALLRQLHIIRNELKQALTE 441
+L R E V N +L + + IR +L Q +E
Sbjct: 1376 TKLAAAWRTKYEQVVNKSLEKH-NQIRQQLSQKTSE 1410
>SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/68 (26%), Positives = 33/68 (48%)
Frame = +1
Query: 136 VIKAELRFKEELQAVAEQVEIMKTLHLTDITRLKSEIEELKHKRSTPTNNYERNEQATLQ 315
+++AE KEE + + VE+MKTLH ++ + I L N ++ +
Sbjct: 872 LLEAEKIEKEE-EKIERVVEVMKTLHPAHLSVFRFLIAHLGRVCKHAEKNLMNSKNVSTV 930
Query: 316 WAKTSMKE 339
+A T M++
Sbjct: 931 FAPTLMRD 938
>SPCC1919.07 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 206
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/48 (27%), Positives = 24/48 (50%)
Frame = -1
Query: 225 NVSKVQSFHDLDLFSDGLQLFLEPQLGLDHGENKK*SQESTNFRAQHD 82
N+S ++ D+D F + + P + D GEN S S+ +++D
Sbjct: 138 NISHAEAEEDIDSFLESMDQSAPPSITEDKGENYISSNHSSMHISRND 185
>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
Mad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 25.8 bits (54), Expect = 5.5
Identities = 22/84 (26%), Positives = 40/84 (47%), Gaps = 2/84 (2%)
Frame = +1
Query: 118 LFLVFTVIKAELRFKEELQAVAEQVEIMKTLHLTDITRLKSEIEELKHKRSTPTNNYERN 297
LF V E +E++ + + ++ L +T + S L+H RS PT YER
Sbjct: 482 LFQVKEKTNIEAFLREQIITLESSIATLRQ-ELAQVTEINS-CRVLQH-RSNPTLKYERI 538
Query: 298 EQATLQW--AKTSMKELXMEIREI 363
+ A L+ A+ S + +E +++
Sbjct: 539 KAAQLEMLNAENSALKALLEDKKV 562
>SPBC1706.01 |tea4|wsh3|tip elongation aberrant protein
Tea4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 809
Score = 25.8 bits (54), Expect = 5.5
Identities = 14/51 (27%), Positives = 23/51 (45%)
Frame = +3
Query: 387 PPPSTAHHT**AKASPYRDLGLSATCSHSRSQATQDGQ*SWQAQIRRTTDE 539
PP T A + +R ++ TCS + S D + ++A + RT E
Sbjct: 202 PPLDIVEKTLEAPSPNFRIKRVTFTCSSNSSDDEMDSENDYEAMVNRTVAE 252
>SPAC20G4.05c |||UPF0061 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 568
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/54 (24%), Positives = 27/54 (50%)
Frame = +1
Query: 292 RNEQATLQWAKTSMKELXMEIREIGESVNNSALLRQLHIIRNELKQALTETSDL 453
RN+ + W + + +E+ + V++ + QLH + LK+A TS++
Sbjct: 325 RNQPDIIIWNLSKLASALVELIGACDKVDDLQYMEQLHNSTDLLKKAFAYTSEV 378
>SPAC4A8.10 |||lipase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 723
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +3
Query: 81 DHVVPENLWTLDFISCFHRDQGRVEV 158
+H PENLW L + S FH + V
Sbjct: 223 EHQTPENLWRLPY-SSFHSKNSHLVV 247
>SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 396
Score = 25.4 bits (53), Expect = 7.2
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +1
Query: 397 QLHIIRNELKQALTETSDLAQLARTQEARLHKMDNEVGRLKYEGQQM 537
Q H + N+ + A+T +DLA+ + ARL ++ N + K +Q+
Sbjct: 50 QFHQVNNKDQSAMTNDTDLAK----RIARLRRVHNAYSKFKSLSEQI 92
>SPCC338.07c |||NatA N-acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 729
Score = 25.4 bits (53), Expect = 7.2
Identities = 12/25 (48%), Positives = 20/25 (80%), Gaps = 2/25 (8%)
Frame = +1
Query: 376 NNSALLRQLHIIRNELKQ--ALTET 444
NNS+LLR L +++++L+Q AL +T
Sbjct: 110 NNSSLLRDLALLQSQLRQYKALADT 134
>SPBC23G7.12c |rpt6|let1|19S proteasome regulatory subunit
Rpt6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 403
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +1
Query: 1 DSSPELWSRQTTRRSRVFTRNNSKSIVIMLCPKICGLLTLFLV 129
D SP++ ++ RV RN+S ++ +L K+ L++L +V
Sbjct: 94 DISPDIDIKEIKPNIRVALRNDSYQLIKILPNKVDPLVSLMMV 136
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,444,492
Number of Sequences: 5004
Number of extensions: 45741
Number of successful extensions: 138
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 137
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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