Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_D20
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 87 1e-17
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 84 9e-17
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 81 5e-16
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 79 3e-15
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 36 0.018
At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK... 30 1.5
At5g38990.1 68418.m04717 protein kinase family protein contains ... 29 3.5
At1g70330.1 68414.m08091 equilibrative nucleoside transporter fa... 29 3.5
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 4.7
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 8.1
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 86.6 bits (205), Expect = 1e-17
Identities = 57/171 (33%), Positives = 82/171 (47%), Gaps = 11/171 (6%)
Frame = +2
Query: 110 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-----ASYTPAE 274
L L+++L +SY++GY S+ D+ VF + K P + +V RW+N I S AE
Sbjct: 13 LKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDALLRISGVSAE 72
Query: 275 RK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXXXXXXXXXXXL 436
+G+SP+ VDLFG
Sbjct: 73 GSGVIVEGSSPITEEAVATPPAADSKDTAAEEEDDDDVDLFGEETEEEKKAAEE------ 126
Query: 437 KAYADKKSKKPALIAKSSILLDVKPWDDETDMXEMENQVRTIEMEGLLWGS 589
+A + K S K KSS+L+D+KPWDDETDM ++E VR+I+MEGL WG+
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLFWGA 177
Score = 37.1 bits (82), Expect = 0.010
Identities = 16/19 (84%), Positives = 16/19 (84%)
Frame = +1
Query: 592 SKLVPVGYGINKLQIMCVI 648
SKLVPVGYGI KL IMC I
Sbjct: 178 SKLVPVGYGIKKLHIMCTI 196
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 83.8 bits (198), Expect = 9e-17
Identities = 55/171 (32%), Positives = 81/171 (47%), Gaps = 11/171 (6%)
Frame = +2
Query: 110 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQI-----ASYTPAE 274
L L+++L +SY++GY S+ D+ VF + K P + + RWYN I S AE
Sbjct: 13 LKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDALLRISGVSAE 72
Query: 275 RK-TWSQGTSPLXXXXXXXXXXXXXXXXXXXX-----VDLFGSGXXXXXXXXXXXXXXXL 436
+G++P+ VDLFG
Sbjct: 73 GSGVIVEGSAPITEEAVATPPAADSKDAAADEEDDDDVDLFGEETEEEKKAAEE------ 126
Query: 437 KAYADKKSKKPALIAKSSILLDVKPWDDETDMXEMENQVRTIEMEGLLWGS 589
+A + K S K KSS+L+D+KPWDDETDM ++E V++I+MEGL WG+
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLFWGA 177
Score = 37.9 bits (84), Expect = 0.006
Identities = 16/19 (84%), Positives = 17/19 (89%)
Frame = +1
Query: 592 SKLVPVGYGINKLQIMCVI 648
SKLVPVGYGI KLQI+C I
Sbjct: 178 SKLVPVGYGIKKLQILCTI 196
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 81.4 bits (192), Expect = 5e-16
Identities = 51/163 (31%), Positives = 73/163 (44%), Gaps = 3/163 (1%)
Frame = +2
Query: 110 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTPAE---RK 280
+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS +
Sbjct: 14 VKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVASQLAKSFPGKA 73
Query: 281 TWSQGTSPLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLKAYADKKS 460
Q +DLFG + A K +
Sbjct: 74 VGVQFGGSAAAAPAVEAEAPAAAADDDDDMDLFGDETEEEKKAAEE------REAAKKDT 127
Query: 461 KKPALIAKSSILLDVKPWDDETDMXEMENQVRTIEMEGLLWGS 589
KKP KSS+L+DVKPWDDETDM ++E VR +EM GL WG+
Sbjct: 128 KKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGA 170
Score = 32.3 bits (70), Expect = 0.29
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = +1
Query: 592 SKLVPVGYGINKLQIMCVI 648
SKLVPVGYGI KL IM I
Sbjct: 171 SKLVPVGYGIKKLTIMFTI 189
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 79.0 bits (186), Expect = 3e-15
Identities = 51/167 (30%), Positives = 75/167 (44%), Gaps = 7/167 (4%)
Frame = +2
Query: 110 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTPAERKTWS 289
L L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+ +
Sbjct: 14 LKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASHLAKSFPGKA 73
Query: 290 QGTS-------PLXXXXXXXXXXXXXXXXXXXXVDLFGSGXXXXXXXXXXXXXXXLKAYA 448
G P +DLF + A
Sbjct: 74 DGVRVGGGVAPPSEAHPHTEEPAADGDGDDDDDIDLFADETEDEKKAAEE------REAA 127
Query: 449 DKKSKKPALIAKSSILLDVKPWDDETDMXEMENQVRTIEMEGLLWGS 589
K +KK KSS+LL+VKPWDDETDM ++E VR+++M GL WG+
Sbjct: 128 KKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGA 174
Score = 32.7 bits (71), Expect = 0.22
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = +1
Query: 592 SKLVPVGYGINKLQIMCVI 648
SKLVPVGYGI KL IM I
Sbjct: 175 SKLVPVGYGIKKLTIMMTI 193
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 36.3 bits (80), Expect = 0.018
Identities = 24/58 (41%), Positives = 30/58 (51%), Gaps = 9/58 (15%)
Frame = +2
Query: 107 ALNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQI 253
AL LN LA KS + +G TPS ADV VF + + +PHV+RW N I
Sbjct: 75 ALEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g64960.1 68418.m08171 cyclin-dependent kinase, putative / CDK,
putative similar to cyclin dependent kinase C
[Lycopersicon esculentum] gi|15215944|emb|CAC51391
Length = 513
Score = 29.9 bits (64), Expect = 1.5
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +2
Query: 125 QYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLR--WYNQIASYTPAERK 280
+ L K + G T ++ +++E G +N P V + WYNQ+ S P +R+
Sbjct: 233 ELLNGKPILPGKTENEQLNKIYELCGSPDESNWPGVSKMPWYNQMKSSRPLKRR 286
>At5g38990.1 68418.m04717 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 880
Score = 28.7 bits (61), Expect = 3.5
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +3
Query: 222 FPTYYDGIIKLLHTLRLNAKLGLRAPAH*PPVLNPRLPPQ 341
+PTYYD I+ + L+L+ G A + P L+P PPQ
Sbjct: 387 YPTYYDAILSGVEILKLSNSDGNLAGLNPIPQLSP--PPQ 424
>At1g70330.1 68414.m08091 equilibrative nucleoside transporter
family protein contains similarity to SWISS-PROT:Q14542
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type,
36 kDa nucleolar protein HNP36, Hydrophobic nucleolar
protein, 36 kDa, Delayed-early response protein 12)
[Homo sapiens]
Length = 450
Score = 28.7 bits (61), Expect = 3.5
Identities = 9/25 (36%), Positives = 17/25 (68%)
Frame = -3
Query: 583 PEKAFHFNSAYLVFHFXHIGFIIPW 509
P ++HF AY+++ +GF++PW
Sbjct: 57 PSDSYHF--AYIIYFTLGVGFLLPW 79
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +2
Query: 110 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 205
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 8.1
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 110 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 223
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,633,230
Number of Sequences: 28952
Number of extensions: 234141
Number of successful extensions: 571
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 552
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 570
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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