Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_D06
(493 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 69 5e-13
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 64 1e-11
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 63 3e-11
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 51 1e-07
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 41 9e-05
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 38 0.001
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 36 0.003
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 30 0.16
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 29 0.29
SPBC14C8.16c |bot1||mitochondrial ribosomal protein subunit S35 ... 28 0.88
SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 0.88
SPCC1494.01 ||SPCC965.15|iron/ascorbate oxidoreductase family|Sc... 27 1.5
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 26 2.7
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 25 6.2
SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 25 8.2
SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 8.2
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 8.2
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 68.5 bits (160), Expect = 5e-13
Identities = 38/107 (35%), Positives = 56/107 (52%), Gaps = 11/107 (10%)
Frame = +3
Query: 201 FXTEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDIY 380
+ E+EIGE ++ E V R DI++T+KLW HR V A+ +L+ L L+YID Y
Sbjct: 59 YGNEKEIGEGIR----ESGVPRTDIWVTSKLWCNAHRAGLVPLALEKTLQDLNLEYIDAY 114
Query: 381 LMHWPIGL-----------NADYSHSDVDFMETWRGLEDAQRMGIVK 488
L+HWP L + + DV ETW+ +E+ G V+
Sbjct: 115 LIHWPFALLSGPEELPRNEKGELIYEDVPIEETWQAMEELLETGKVR 161
Score = 34.3 bits (75), Expect = 0.010
Identities = 18/52 (34%), Positives = 28/52 (53%)
Frame = +2
Query: 53 NGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFXTASVFXN 208
NG +P+IGLGT+ +S + ++ V A+ GYRH TA ++ N
Sbjct: 19 NGDKIPSIGLGTW---------RSGKDETKNAVCAALKAGYRHIDTAHIYGN 61
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 64.1 bits (149), Expect = 1e-11
Identities = 37/107 (34%), Positives = 54/107 (50%), Gaps = 11/107 (10%)
Frame = +3
Query: 201 FXTEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDIY 380
+ E E+G+ +K E V R+DI++T+KLW H V +A+ +LK L LDY+D Y
Sbjct: 60 YGNEDEVGDGIK----ESGVPRKDIWVTSKLWCNAHAPEAVPKALEKTLKDLKLDYLDEY 115
Query: 381 LMHWPIGLNA--DYSHSDVD---------FMETWRGLEDAQRMGIVK 488
L+HWP+ D D D ETW+ +E G V+
Sbjct: 116 LIHWPVSFKTGEDKFPKDKDGNLIYEKNPIEETWKAMEKLLETGKVR 162
Score = 33.1 bits (72), Expect = 0.023
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +2
Query: 53 NGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFXTASVFXN 208
+G +P +GLGT+ +S+ Q ++ V A+ GYRH A+++ N
Sbjct: 20 DGSKIPGLGLGTW---------RSEPNQTKNAVKTALQYGYRHIDAAAIYGN 62
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 62.9 bits (146), Expect = 3e-11
Identities = 34/95 (35%), Positives = 54/95 (56%)
Frame = +3
Query: 201 FXTEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDIY 380
+ E+E+G A+K E V R +FIT+K+ H + +A+N+SL+KLG DY+D+Y
Sbjct: 54 YGNEEEVGVALK----EANVPRSKLFITSKV---MHNVDNIPEALNESLRKLGTDYLDLY 106
Query: 381 LMHWPIGLNADYSHSDVDFMETWRGLEDAQRMGIV 485
L+H PI + + E W+ +E A G+V
Sbjct: 107 LLHSPI----PFYEKKIPISEGWKAMETALGTGLV 137
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 50.8 bits (116), Expect = 1e-07
Identities = 31/93 (33%), Positives = 51/93 (54%)
Frame = +3
Query: 201 FXTEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDIY 380
+ E + G A+ +EE KREDI+ T+KL + ++ ++ +I+ S+K GL YID++
Sbjct: 50 YHNEADCGRAILKFMEETGTKREDIWFTSKLNDLSGYKSTLS-SIDASVKACGLGYIDLF 108
Query: 381 LMHWPIGLNADYSHSDVDFMETWRGLEDAQRMG 479
L+H P G D +E+W+ LE G
Sbjct: 109 LLHSPYG----------DRIESWKALEKGVEEG 131
Score = 29.1 bits (62), Expect = 0.38
Identities = 18/56 (32%), Positives = 28/56 (50%)
Frame = +2
Query: 41 LKMNNGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFXTASVFXN 208
+K+NNG P G+Y+ V ++K D V A+ GYRH +A ++ N
Sbjct: 6 VKLNNGLKCPQFAYGSYM------VNRTK---CFDSVYAALQCGYRHIDSAQMYHN 52
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 41.1 bits (92), Expect = 9e-05
Identities = 27/67 (40%), Positives = 37/67 (55%)
Frame = +3
Query: 201 FXTEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDIY 380
+ E G+A+ E+ VKR DIF+T+KL N + A AI SL LG YID++
Sbjct: 55 YGNEDICGKAIVDWCEKNNVKRTDIFLTSKLANCSDYYSTRA-AIRSSLHHLG-TYIDLF 112
Query: 381 LMHWPIG 401
L+ P G
Sbjct: 113 LIQSPAG 119
Score = 32.7 bits (71), Expect = 0.031
Identities = 18/54 (33%), Positives = 28/54 (51%)
Frame = +2
Query: 47 MNNGRDMPAIGLGTYLGFDQNGVVKSKDKQLRDVVMRAIDVGYRHFXTASVFXN 208
+ NG +P IG G ++ K + +V +A+D GYRH TA+V+ N
Sbjct: 13 LTNGMVIPRIGFGAFM---------LKYNECYGLVTQALDSGYRHIDTAAVYGN 57
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 37.5 bits (83), Expect = 0.001
Identities = 20/70 (28%), Positives = 39/70 (55%), Gaps = 4/70 (5%)
Frame = +3
Query: 201 FXTEQEIGEAVKMKVEEGV-VKREDIFITTKLWNTQHRRTEVAQAINDSLKKLGLDYIDI 377
+ E+E +A++ + V +KREDI + TK+ ++ +A+ SL++ G +D+
Sbjct: 47 YRNEKECEQAIQDFCHQNVNIKREDITLITKIPDSLQGFERTWKAVEQSLRRTGRPKLDV 106
Query: 378 YLMH---WPI 398
L+H WP+
Sbjct: 107 VLIHSPKWPV 116
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 35.9 bits (79), Expect = 0.003
Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 1/38 (2%)
Frame = +3
Query: 285 TKLWNTQH-RRTEVAQAINDSLKKLGLDYIDIYLMHWP 395
TKL NT R + + +N SLK+LGL Y+D+ + H P
Sbjct: 102 TKLPNTTGLSRKHIIEGLNASLKRLGLPYVDVIMAHRP 139
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 30.3 bits (65), Expect = 0.16
Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 10/53 (18%)
Frame = +3
Query: 261 KREDIFITTKLWNTQHRRTE----------VAQAINDSLKKLGLDYIDIYLMH 389
+R++IF+ TK ++ T + +A++ SLK+LG+D ID+Y +H
Sbjct: 74 RRKEIFLATKFGYEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYVH 126
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 29.5 bits (63), Expect = 0.29
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -2
Query: 411 RHLIQWASASNKYLYNPDLVSSV 343
+HL +W S+S YLY+P L S V
Sbjct: 1780 QHLARWISSSKSYLYSPLLSSHV 1802
>SPBC14C8.16c |bot1||mitochondrial ribosomal protein subunit S35
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 315
Score = 27.9 bits (59), Expect = 0.88
Identities = 14/56 (25%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
Frame = +3
Query: 195 PFFXTEQEIGEAVKMKVEEGVVKREDIFITTKLWNTQHRRTEV---AQAINDSLKK 353
PFF + I +++MK+ + ++ I + + +NT +R E + IND ++
Sbjct: 124 PFFKPPRPISHSLRMKITDEYLQGASIEVLARKFNTSPQRIEALIKLRRINDEFEE 179
>SPAC25A8.02 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 390
Score = 27.9 bits (59), Expect = 0.88
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = +3
Query: 285 TKLWNTQHRRTEVAQ-AINDSLKKLGLDYIDIYLMHW 392
+K + T +RR+E+ Q +++ KKL +D+Y +HW
Sbjct: 125 SKEYLTAYRRSELLQNKLHEYQKKLITRVLDMYQLHW 161
>SPCC1494.01 ||SPCC965.15|iron/ascorbate oxidoreductase
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 321
Score = 27.1 bits (57), Expect = 1.5
Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 4/33 (12%)
Frame = +3
Query: 186 IPPPFFX----TEQEIGEAVKMKVEEGVVKRED 272
IPP FF +E+++ +K + EGV +RED
Sbjct: 147 IPPDFFSKSHSSEEDVLRLLKYSIPEGVERRED 179
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 26.2 bits (55), Expect = 2.7
Identities = 9/30 (30%), Positives = 19/30 (63%)
Frame = +3
Query: 285 TKLWNTQHRRTEVAQAINDSLKKLGLDYID 374
TK+WN H+ E + ++D++++ L +D
Sbjct: 680 TKIWNCVHQLLEREKKMSDAMRQTKLTIVD 709
>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1323
Score = 25.0 bits (52), Expect = 6.2
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +2
Query: 17 RQRWKCPRLKMNNGRDMPAIGLGTYLGFDQN 109
R +C R +M N D GLG +L FD N
Sbjct: 1017 RDNPECARQEMENIADNNDPGLGYHLTFDPN 1047
>SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 630
Score = 24.6 bits (51), Expect = 8.2
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 335 KRFTEETRSGLYRYLFDALAHWIK 406
K T+E SG YRY FD W++
Sbjct: 587 KMITDEFESGSYRY-FDFEGDWVQ 609
>SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1628
Score = 24.6 bits (51), Expect = 8.2
Identities = 12/41 (29%), Positives = 21/41 (51%)
Frame = -3
Query: 125 WTSLRRSDQTQGTCPGRWRACLCRCSFLXEGTSIVDASPSP 3
W L SD+ G G+++ L +F +G ++ A+P P
Sbjct: 337 WAHLSPSDEELGKELGKYKDKLDEQTFKAKGNALTMAAPLP 377
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 24.6 bits (51), Expect = 8.2
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = -1
Query: 295 HSLVVIKISSLLTTPSSTFIFTASPISCSVXKNGGGXEMTVSDIYGAHYNIT 140
+S VI SS L + SS+ I ++SP++ S VS + NIT
Sbjct: 666 YSSSVIPSSSTLVSSSSSLIVSSSPVASSSSSPIPSSSSLVSTYSASLSNIT 717
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,002,717
Number of Sequences: 5004
Number of extensions: 38611
Number of successful extensions: 123
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 192109570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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