Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_C06
(650 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 70 3e-13
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 53 3e-08
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 41 2e-04
SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr 2|||Ma... 35 0.012
SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy... 30 0.25
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 28 1.3
SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit ... 27 1.8
SPAPB17E12.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.3
SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces pom... 26 5.4
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 25 7.2
SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr... 25 9.5
SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po... 25 9.5
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 69.7 bits (163), Expect = 3e-13
Identities = 49/118 (41%), Positives = 68/118 (57%), Gaps = 5/118 (4%)
Frame = +2
Query: 308 LKDGTLLCKLANNIHPNMIKKINTSSMAFKCMENINAFLEAARQL-GVPAQETFQTVDLW 484
L+ G +LC++ I+ S+M F MENI+AF+ A+Q+ VP+Q+ FQT DL+
Sbjct: 31 LQSGVILCRICKEALGANIR-YKESNMPFVQMENISAFINYAQQVVHVPSQDMFQTSDLF 89
Query: 485 ERQNLNSVVICLQSLGRKAGTY--GK-PSIGPKEAEKNVRNFSEEQLRA-GQGVISLQ 646
ER+N V+ + S R A GK +GPK AEK R FS +Q R +GV SLQ
Sbjct: 90 ERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLAEKKPRVFSAQQQREFREGVNSLQ 147
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 53.2 bits (122), Expect = 3e-08
Identities = 26/87 (29%), Positives = 47/87 (54%), Gaps = 1/87 (1%)
Frame = +2
Query: 272 TPQGDMDNFYEVLKDGTLLCKLANNIHPN-MIKKINTSSMAFKCMENINAFLEAARQLGV 448
T G F + L++G +L L P+ +IK ++ + F+ +NIN FL+ +G+
Sbjct: 57 TDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHGIGL 116
Query: 449 PAQETFQTVDLWERQNLNSVVICLQSL 529
P F+ D++E +NL V+ C+ +L
Sbjct: 117 PEIFHFELTDIYEGKNLPKVIYCIHAL 143
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 40.7 bits (91), Expect = 2e-04
Identities = 23/83 (27%), Positives = 42/83 (50%), Gaps = 1/83 (1%)
Frame = +2
Query: 284 DMDNFYEVLKDGTLLCKLANNIHPNMIKKINTS-SMAFKCMENINAFLEAARQLGVPAQE 460
++D+F + L +G +LC+LA +P + ++ + +NAF +G+
Sbjct: 84 NLDDFVDALVNGKVLCQLAFKYYPKLASNWKPRYQISERNTVYLNAFFHFLDFIGMFTPF 143
Query: 461 TFQTVDLWERQNLNSVVICLQSL 529
F+T DL R N+ V+ CL +L
Sbjct: 144 RFETKDLVRRFNIPKVIYCLHAL 166
>SPBC1778.06c |fim1||fimbrin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 614
Score = 34.7 bits (76), Expect = 0.012
Identities = 20/57 (35%), Positives = 32/57 (56%), Gaps = 7/57 (12%)
Frame = +2
Query: 296 FYEVLKDGTLLCKLANNIHPNMI------KKINTSSMA-FKCMENINAFLEAARQLG 445
F++ KDG +L KL N+ P+ I K+ N + FKC+EN N + +A+ +G
Sbjct: 145 FFDQCKDGLILSKLINDSVPDTIDERVLNKQRNNKPLDNFKCIENNNVVINSAKAMG 201
Score = 30.3 bits (65), Expect = 0.25
Identities = 18/59 (30%), Positives = 31/59 (52%), Gaps = 8/59 (13%)
Frame = +2
Query: 293 NFYEVLKDGTLLCKLANNIHPNMI--KKINTS------SMAFKCMENINAFLEAARQLG 445
+F+ L+DG +L + + I PN + KK+N + M FK +EN N ++ + G
Sbjct: 406 DFFNNLRDGLILLQAYDKITPNTVNWKKVNKAPASGDEMMRFKAVENCNYAVDLGKNQG 464
>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 991
Score = 30.3 bits (65), Expect = 0.25
Identities = 19/89 (21%), Positives = 35/89 (39%)
Frame = +2
Query: 365 KKINTSSMAFKCMENINAFLEAARQLGVPAQETFQTVDLWERQNLNSVVICLQSLGRKAG 544
++ +S A E + AF+E A+Q G+P E W+ + + +L +
Sbjct: 118 RRFRSSREAALKEEELQAFIEEAKQQGIPIDENATKKKSWDSNCITPGTPFMDTLAKSLR 177
Query: 545 TYGKPSIGPKEAEKNVRNFSEEQLRAGQG 631
Y + +NVR + G+G
Sbjct: 178 YYIINKLNSDPCWRNVRFILSDASVPGEG 206
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 27.9 bits (59), Expect = 1.3
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = +1
Query: 421 PRSRKTVGCTGTGNFSNCRPVGETESQLRRDLLAVTGQKGWNLR 552
P+ + V CTG G+ V +S D L +TG+ G L+
Sbjct: 119 PKFKNIVDCTGAGDVDTSVEVAAADS---NDYLTITGRSGRTLK 159
>SPCC1919.14c |bdp1||transcription factor TFIIIB complex subunit
Bdp1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 507
Score = 27.5 bits (58), Expect = 1.8
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 3/66 (4%)
Frame = +1
Query: 256 TGEPENTSGRYGQLLRSA*RRHSSVQASQQHPPEH--DQ-KDQHIVDGLQVHGEYQRFPR 426
+ EP+ G L +S++ S HP + DQ D+ VDGL + Y PR
Sbjct: 253 SSEPKEKEEIVGSLEEEGTDESASLEDSLSHPTKSILDQLADKMEVDGLNNNSNYSATPR 312
Query: 427 SRKTVG 444
+R G
Sbjct: 313 TRVVNG 318
>SPAPB17E12.09 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 203
Score = 27.1 bits (57), Expect = 2.3
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 1/98 (1%)
Frame = +2
Query: 308 LKDGTLLCKLANNIHPNMIKKINTSSMAFKCMENI-NAFLEAARQLGVPAQETFQTVDLW 484
LK+ T + L+++IHPN +++ S +N+ N E + L + + VD +
Sbjct: 6 LKENTEIINLSSSIHPNRDSYLDSQSDPLN--QNLYNIETENVKDLNI------EDVDYY 57
Query: 485 ERQNLNSVVICLQSLGRKAGTYGKPSIGPKEAEKNVRN 598
E+ L + I +++ TY K S+G +N N
Sbjct: 58 EK--LQNFKIVDENIDPGLRTYSKRSVGVNNTFQNPCN 93
>SPAC821.13c ||SPAC955.01c|P-type ATPase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1562
Score = 25.8 bits (54), Expect = 5.4
Identities = 19/63 (30%), Positives = 24/63 (38%), Gaps = 1/63 (1%)
Frame = +3
Query: 39 PVDPPACLHSVHSDFTFYRVCDLFK*FIKLLLVRNGKQ-PSDQIRICS*SPEKDQQQIQR 215
P PP L S H +FT F+ L R+ + S RI +P D QI
Sbjct: 166 PPIPPEFLKSRHKEFTIPNPLQFISNFLSNLFSRDTRYLKSSHGRIIIINPYDDSSQIDE 225
Query: 216 GAG 224
G
Sbjct: 226 RTG 228
>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 879
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/38 (28%), Positives = 18/38 (47%)
Frame = -2
Query: 457 LCRYTQLSCGFEESVDILHALEGHRRCVDLFDHVRVDV 344
L R+ LS +D++ L+G + C HV D+
Sbjct: 67 LARWVHLSPSVHRCIDVVAFLQGQKFCFQNLVHVLQDI 104
>SPAC16E8.14c |||methyltransferase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -2
Query: 169 LIWSLGCLPFRTNNNLINYLNK 104
LIW+ CL T+ +LI YL++
Sbjct: 123 LIWNQWCLSHLTDEDLIAYLSR 144
>SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 361
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = -1
Query: 260 PVIIRIHSKDSWASSSLYLL 201
P IIR H +D W S + LL
Sbjct: 331 PCIIRSHKEDDWVSDIVSLL 350
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,887,058
Number of Sequences: 5004
Number of extensions: 61414
Number of successful extensions: 187
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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