Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_B20
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 212 2e-55
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 207 4e-54
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 207 4e-54
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 206 8e-54
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 204 5e-53
At5g50100.1 68418.m06204 expressed protein contains Pfam domain ... 28 6.2
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot... 28 6.2
At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (... 28 6.2
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 212 bits (518), Expect = 2e-55
Identities = 103/170 (60%), Positives = 125/170 (73%), Gaps = 2/170 (1%)
Frame = +3
Query: 147 FLFTSESVGEGHPDKMCDQISDAILXAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 326
FLFTSESV EGHPDK+CDQISDAIL A L QDP++KVACET TKT MV++ GEIT+KANV
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 327 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALXQQSPNIAAGVHXN--RNDEEVGAGDX 500
DY+++VR+T + IG+ + G D C V++ + QQSP+IA GVH + + EEVGAGD
Sbjct: 64 DYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEVGAGDQ 123
Query: 501 GLMFGYATXXTEXCMPLTVVLAHKLNXKIAELXRNGXFWWARPXSKTQVT 650
G MFGYAT T MPLT VLA KL K+ E+ +NG W RP KTQVT
Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVT 173
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 207 bits (506), Expect = 4e-54
Identities = 100/170 (58%), Positives = 123/170 (72%), Gaps = 2/170 (1%)
Frame = +3
Query: 147 FLFTSESVGEGHPDKMCDQISDAILXAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 326
FLFTSESV EGHPDK+CDQISDA+L A L QDPD+KVACET TKT MV++ GEIT+KA V
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 327 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALXQQSPNIAAGVHXN--RNDEEVGAGDX 500
DY+K+VR+T + IG+ G D C V++ + QQSP+IA GVH + + EE+GAGD
Sbjct: 64 DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123
Query: 501 GLMFGYATXXTEXCMPLTVVLAHKLNXKIAELXRNGXFWWARPXSKTQVT 650
G MFGYAT T MPL+ VLA KL ++ E+ +NG W RP KTQVT
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVT 173
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 207 bits (506), Expect = 4e-54
Identities = 100/170 (58%), Positives = 123/170 (72%), Gaps = 2/170 (1%)
Frame = +3
Query: 147 FLFTSESVGEGHPDKMCDQISDAILXAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 326
FLFTSESV EGHPDK+CDQISDA+L A L QDPD+KVACET TKT MV++ GEIT+KA V
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63
Query: 327 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALXQQSPNIAAGVHXN--RNDEEVGAGDX 500
DY+K+VR+T + IG+ G D C V++ + QQSP+IA GVH + + EE+GAGD
Sbjct: 64 DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123
Query: 501 GLMFGYATXXTEXCMPLTVVLAHKLNXKIAELXRNGXFWWARPXSKTQVT 650
G MFGYAT T MPL+ VLA KL ++ E+ +NG W RP KTQVT
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVT 173
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 206 bits (504), Expect = 8e-54
Identities = 97/170 (57%), Positives = 123/170 (72%), Gaps = 2/170 (1%)
Frame = +3
Query: 147 FLFTSESVGEGHPDKMCDQISDAILXAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 326
FLFTSESV EGHPDK+CDQISDA+L A L QDPD+KVACET TKT MV++ GEIT+KA +
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63
Query: 327 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALXQQSPNIAAGVHXN--RNDEEVGAGDX 500
DY+K+VR+T + IG+ G D C V++ + QQSP+IA GVH + + E++GAGD
Sbjct: 64 DYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQ 123
Query: 501 GLMFGYATXXTEXCMPLTVVLAHKLNXKIAELXRNGXFWWARPXSKTQVT 650
G MFGYAT T MPL+ VLA K+ ++ E+ +NG W RP KTQVT
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVT 173
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 204 bits (497), Expect = 5e-53
Identities = 99/170 (58%), Positives = 122/170 (71%), Gaps = 2/170 (1%)
Frame = +3
Query: 147 FLFTSESVGEGHPDKMCDQISDAILXAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 326
FLFTSESV EGHPDK+CDQISDAIL A L QDP++KVACET TKT MV++ GEIT+ A V
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKV 63
Query: 327 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALXQQSPNIAAGVHXN--RNDEEVGAGDX 500
DY+K+VR T + IG+ + G D C+V++ + QQSP+IA GVH + + E++GAGD
Sbjct: 64 DYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQ 123
Query: 501 GLMFGYATXXTEXCMPLTVVLAHKLNXKIAELXRNGXFWWARPXSKTQVT 650
G MFGYAT T MPLT VLA KL K+ E+ +N W RP KTQVT
Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVT 173
>At5g50100.1 68418.m06204 expressed protein contains Pfam domain
PF04134: Protein of unknown function, DUF393
Length = 214
Score = 27.9 bits (59), Expect = 6.2
Identities = 15/41 (36%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +3
Query: 375 DSSKGFDYKTCSVMLALXQQSPNIAAGVH-XNRNDEEVGAG 494
+ ++G DYKT + Q N+ GV R EEVG G
Sbjct: 116 EDNQGLDYKTVMGQIHAIQSDGNVVKGVEAFRRLYEEVGLG 156
>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
nucleotide-exchange protein 2 {Homo sapiens}; contains
Pfam profile PF01369: Sec7 domain
Length = 1756
Score = 27.9 bits (59), Expect = 6.2
Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Frame = +3
Query: 240 DPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRE---TVKHIGYDDSSKGFDYKTCS 410
DP+ +A +T + G L C K+ VD++K+ RE + ++ D +S G +T S
Sbjct: 523 DPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKS 582
>At1g54960.1 68414.m06277 NPK1-related protein kinase, putative
(ANP2) similar to protein kinase [Nicotiana tabacum]
gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
protein kinase 2, partial cds GI:2342424
Length = 596
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/49 (26%), Positives = 25/49 (51%)
Frame = +3
Query: 231 LNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDD 377
L+ + DAK +C+T+++ +L C + N + + V V H Y +
Sbjct: 431 LDNNTDAKKSCDTMSEISDILKCKFDENSGNGETETKVSMEVDHPSYSE 479
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,024,116
Number of Sequences: 28952
Number of extensions: 283294
Number of successful extensions: 630
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 613
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 625
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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