Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_B07
(621 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 136 2e-33
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 71 2e-13
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 60 3e-10
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 54 2e-08
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 36 0.005
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 36 0.006
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 34 0.019
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca... 27 1.7
SPBC1683.09c |frp1||ferric-chelate reductase Frp1|Schizosaccharo... 27 2.9
SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces p... 23 3.1
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 3.8
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 26 5.1
SPAC1834.06c |pmo25||mo25 family protein Pmo25|Schizosaccharomyc... 25 6.7
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe... 25 6.7
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 25 6.7
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 136 bits (329), Expect = 2e-33
Identities = 59/65 (90%), Positives = 60/65 (92%)
Frame = +2
Query: 236 PAIRAVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 415
P +MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA
Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97
Query: 416 PEEHP 430
PEEHP
Sbjct: 98 PEEHP 102
Score = 78.2 bits (184), Expect = 9e-16
Identities = 33/40 (82%), Positives = 37/40 (92%)
Frame = +1
Query: 130 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 249
+++ LV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 3 EEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 66.5 bits (155), Expect = 3e-12
Identities = 30/40 (75%), Positives = 32/40 (80%)
Frame = +3
Query: 417 PRNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQ 536
P LLTEAPLNPK+NREKMTQI+FETFN PA YV IQ
Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQ 137
Score = 41.5 bits (93), Expect = 1e-04
Identities = 20/26 (76%), Positives = 22/26 (84%)
Frame = +1
Query: 529 ASKXVLSLYAXGRTTGIVLDSXDGVS 606
A + VLSLYA GRTTGIVLDS DGV+
Sbjct: 135 AIQAVLSLYASGRTTGIVLDSGDGVT 160
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 70.5 bits (165), Expect = 2e-13
Identities = 31/61 (50%), Positives = 42/61 (68%), Gaps = 1/61 (1%)
Frame = +2
Query: 251 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEH 427
VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 428 P 430
P
Sbjct: 107 P 107
Score = 52.4 bits (120), Expect = 5e-08
Identities = 20/34 (58%), Positives = 27/34 (79%)
Frame = +1
Query: 145 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 246
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
Score = 36.7 bits (81), Expect = 0.003
Identities = 16/40 (40%), Positives = 23/40 (57%)
Frame = +3
Query: 417 PRNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQ 536
P +LLTE PL N+EK+ + +ET N PA+ +Q
Sbjct: 103 PEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQ 142
Score = 33.5 bits (73), Expect = 0.025
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +1
Query: 514 PPCTSASKXVLSLYAXGRTTGIVLDSXDGVS 606
P + + + VL+LYA RTTGIVL+ DG++
Sbjct: 135 PALSFSLQPVLALYASARTTGIVLECGDGLT 165
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 59.7 bits (138), Expect = 3e-10
Identities = 21/49 (42%), Positives = 39/49 (79%)
Frame = +2
Query: 272 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAP 418
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDP 98
Score = 51.2 bits (117), Expect = 1e-07
Identities = 22/40 (55%), Positives = 28/40 (70%)
Frame = +3
Query: 417 PRNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQ 536
PR ++LLTE P+NP ANREKM + MFE + +YV IQ
Sbjct: 98 PRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQ 137
Score = 45.6 bits (103), Expect = 6e-06
Identities = 19/31 (61%), Positives = 24/31 (77%)
Frame = +1
Query: 145 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 237
+V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 6 IVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 33.9 bits (74), Expect = 0.019
Identities = 15/26 (57%), Positives = 21/26 (80%)
Frame = +1
Query: 529 ASKXVLSLYAXGRTTGIVLDSXDGVS 606
A + VLSLYA G ++G+V+DS DGV+
Sbjct: 135 AIQAVLSLYAQGLSSGVVVDSGDGVT 160
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 53.6 bits (123), Expect = 2e-08
Identities = 21/52 (40%), Positives = 31/52 (59%), Gaps = 1/52 (1%)
Frame = +2
Query: 275 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 427
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDH 124
Score = 48.8 bits (111), Expect = 6e-07
Identities = 20/40 (50%), Positives = 26/40 (65%)
Frame = +3
Query: 417 PRNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQ 536
P + LLTE PLNP NRE +IMFE+FN +Y+ +Q
Sbjct: 121 PEDHYFLLTEPPLNPPENRENTAEIMFESFNCAGLYIAVQ 160
Score = 40.7 bits (91), Expect = 2e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 145 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 228
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 145 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 240
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 33.5 bits (73), Expect = 0.025
Identities = 16/57 (28%), Positives = 33/57 (57%), Gaps = 1/57 (1%)
Frame = +2
Query: 263 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 430
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHP 117
Score = 32.7 bits (71), Expect = 0.044
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +3
Query: 432 VLLTEAPLNPKANREKMTQIMFETFNTPAMYVGI 533
+ +TE NP R MT+++FE +N P++ GI
Sbjct: 118 ICMTEPLANPTYVRSTMTELLFELYNAPSVAYGI 151
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 35.5 bits (78), Expect = 0.006
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +1
Query: 130 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSIVG 231
+++P LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
Score = 30.3 bits (65), Expect = 0.23
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = +3
Query: 417 PRNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQXRALA 551
P +++TE NP++ R+++ + FE + PA Y+ Q +A
Sbjct: 99 PTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVA 143
Score = 28.7 bits (61), Expect = 0.72
Identities = 17/66 (25%), Positives = 30/66 (45%), Gaps = 2/66 (3%)
Frame = +2
Query: 236 PAIRAVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 409
P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 410 VAPEEH 427
V P E+
Sbjct: 97 VNPTEY 102
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 33.9 bits (74), Expect = 0.019
Identities = 13/29 (44%), Positives = 19/29 (65%)
Frame = +1
Query: 145 LVVDNGSGMCKAGFAGDDAPRAVFPSIVG 231
+V+D GS + GF+G+D P+ V PS G
Sbjct: 14 IVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
Score = 33.5 bits (73), Expect = 0.025
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 317 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHP 430
+ +K I +G V NWD +W + +L+ P EHP
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHP 100
Score = 33.5 bits (73), Expect = 0.025
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = +3
Query: 417 PRNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQXRALA 551
P +L+TE NP NR K + MFE+ PA Y+ Q A
Sbjct: 96 PLEHPILITEPFDNPPENRVKTLETMFESLRCPATYLAKQETCAA 140
>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 2410
Score = 27.5 bits (58), Expect = 1.7
Identities = 14/43 (32%), Positives = 20/43 (46%)
Frame = -3
Query: 550 ARARXWMPTYMAGVLNVSNMIWVIFSLLALGLRGASVSRTWVF 422
A R W+ A + +++ IF L G GA +R WVF
Sbjct: 2069 APGRVWITNVAAWIYAIASASGSIFFSLNFGEEGAVQTRIWVF 2111
>SPBC1683.09c |frp1||ferric-chelate reductase
Frp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 564
Score = 26.6 bits (56), Expect = 2.9
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +2
Query: 248 AVMVGMGQKDSYVGDEAQSKRGILTLKYPI 337
A+M+G +Y+G AQ KR +LT + I
Sbjct: 164 AIMIGAIHGFAYIGLAAQGKRALLTARVTI 193
>SPBC725.05c |||nucleotide pyrophosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 485
Score = 23.0 bits (47), Expect(2) = 3.1
Identities = 11/27 (40%), Positives = 13/27 (48%)
Frame = +2
Query: 353 TNWDDMEKIWHHTFYNELRVAPEEHPG 433
T WD E IW + N +R A PG
Sbjct: 162 TWWDKGEPIWVNAERNNVRSAVHMWPG 188
Score = 21.8 bits (44), Expect(2) = 3.1
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +2
Query: 314 ILTLKYPIEHGIVTN 358
I+T YP HGIV+N
Sbjct: 125 IVTGLYPESHGIVSN 139
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 3.8
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +3
Query: 435 LLTEAPLNPKANREKMTQIMFETFN 509
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 25.8 bits (54), Expect = 5.1
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = +2
Query: 332 PIEHGIVTNWDDMEKIWHHTF 394
PI+ G V +W+ ++ W H +
Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133
>SPAC1834.06c |pmo25||mo25 family protein Pmo25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 329
Score = 25.4 bits (53), Expect = 6.7
Identities = 14/54 (25%), Positives = 29/54 (53%)
Frame = +3
Query: 420 RNTQVLLTEAPLNPKANREKMTQIMFETFNTPAMYVGIQXRALAVRVRSYHRYR 581
R + LL E LN +ANR MT+ + N M + ++ ++ ++ ++H ++
Sbjct: 223 RQSLKLLGEILLN-RANRSVMTRYISSAENLKLMMILLRDKSKNIQFEAFHVFK 275
>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = -1
Query: 606 GNTVAGVQHDTGGTTXRVQREHGLGCRRTWRAC*MFRT 493
G +AG H T T ++ LG TW +C ++ T
Sbjct: 46 GARIAGCLHMTIQTAVLIETLVALGAEVTWSSCNIYST 83
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 25.4 bits (53), Expect = 6.7
Identities = 19/65 (29%), Positives = 26/65 (40%)
Frame = +1
Query: 130 KKLPRLVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGRYGTEGLLCRR*GTEQK 309
K + RL +D G AGF ++ V H G G + RR G E+
Sbjct: 899 KGISRLGIDRGFSSSGAGFGSGSNYKSAPSRGVSHHGHGGMSGSHRGSQRGSRRGGGERD 958
Query: 310 RYPDP 324
+ PDP
Sbjct: 959 K-PDP 962
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,632,961
Number of Sequences: 5004
Number of extensions: 54687
Number of successful extensions: 155
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 273658928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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