Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_A23
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 81 1e-16
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 63 3e-11
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 60 3e-10
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 57 2e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 43 4e-05
SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 29 0.44
SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 29 0.44
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 29 0.59
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 1.4
SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual 27 2.4
SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 26 4.1
SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2 |Sch... 26 5.5
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 25 7.2
SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 25 7.2
SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|c... 25 9.5
SPCC24B10.12 |||CGI121 family protein|Schizosaccharomyces pombe|... 25 9.5
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 81.4 bits (192), Expect = 1e-16
Identities = 40/105 (38%), Positives = 67/105 (63%), Gaps = 5/105 (4%)
Frame = +1
Query: 346 KNPTEDYLEGMMNEAP----GPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEXNNGVI 513
++PT L+ M+NE G I+FT FLT+ +++ TD E+ ++ AF FD+ NG I
Sbjct: 43 QSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYI 102
Query: 514 GEERLRELLTTMGDRFTDDDVDEMLREAPI-RDGLFDYVEFTRIL 645
E L +LT++G+R + ++V +M+REA DG+ +Y EF+R++
Sbjct: 103 TVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVI 147
Score = 39.9 bits (89), Expect = 3e-04
Identities = 17/36 (47%), Positives = 27/36 (75%)
Frame = +3
Query: 246 QIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLAQKP 353
QIAEF+EAF++ D+++DG I ++L ++ SL Q P
Sbjct: 10 QIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSP 45
Score = 29.1 bits (62), Expect = 0.59
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +3
Query: 231 MFDQAQIAEFKEAFNMIDQNRDGFIDKDDLHDMLASLAQK 350
M D E +EAF + D++ +G+I ++L +L SL ++
Sbjct: 78 MKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 63.3 bits (147), Expect = 3e-11
Identities = 35/124 (28%), Positives = 69/124 (55%), Gaps = 4/124 (3%)
Frame = +1
Query: 286 RTGMDSLTRTICMICLHL*RKNPTEDYLEGMMNEAPGPINFTMFLTLF----GERLQGTD 453
R G + +T L +NPT + + + P ++ FL + G + G D
Sbjct: 17 RHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQFLQVLNRPNGFDMPG-D 75
Query: 454 PEDVIKNAFGCFDEXNNGVIGEERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEF 633
PE+ +K F FD+ G+IG LR +LT++G++ +++++DE+L+ P++DG+ +Y +F
Sbjct: 76 PEEFVKG-FQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHDF 134
Query: 634 TRIL 645
+++
Sbjct: 135 VQMI 138
Score = 28.7 bits (61), Expect = 0.77
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 258 FKEAFNMIDQNRDGFIDKDDLHDMLASLAQKP 353
+K+AF++ D++ G I K + D+L + Q P
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNP 39
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 60.1 bits (139), Expect = 3e-10
Identities = 30/98 (30%), Positives = 54/98 (55%), Gaps = 1/98 (1%)
Frame = +1
Query: 349 NPTEDYLEGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEXNNGVIGEERL 528
N T+ L + NE I+ F++ +L+ T+ E+ AF FD+ N+G I +
Sbjct: 41 NVTDAELAKLSNELGDAIDEKKFMSFVSNKLRETESEEEYIKAFRVFDKDNSGYIETAKF 100
Query: 529 RELLTTMGDRFTDDDVDEMLREA-PIRDGLFDYVEFTR 639
+ + T+G++ +D++V M++EA P G FDY +F +
Sbjct: 101 ADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +3
Query: 246 QIAEFKEAFNMIDQNRDGFIDKDDLHDMLASL 341
Q E KEAF + D ++DG I + +L SL
Sbjct: 7 QTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSL 38
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 57.2 bits (132), Expect = 2e-09
Identities = 31/96 (32%), Positives = 49/96 (51%)
Frame = +1
Query: 346 KNPTEDYLEGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEXNNGVIGEER 525
++ +ED + M PIN FLT G L P + + AF FD+ +G I
Sbjct: 79 QDASEDSINHMFESINPPINLAAFLTAMGSMLCRISPRNDLLEAFSTFDDTQSGKIPIST 138
Query: 526 LRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEF 633
+R+ L++MGDR +V+ +LR + G+F Y +F
Sbjct: 139 MRDALSSMGDRMDPQEVESILR-SYTSHGVFYYEKF 173
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 42.7 bits (96), Expect = 4e-05
Identities = 23/85 (27%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
Frame = +1
Query: 394 GPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEXNNGVIGEERLRELLTTMGDRFTDDD 573
G + F+ + E++ DP + IK AF FD+ G I LR + + + D +
Sbjct: 88 GYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQE 147
Query: 574 VDEMLREAPI-RDGLFDYVEFTRIL 645
++ M+ E + +DG + EF I+
Sbjct: 148 LEAMIEEFDLDQDGEINEQEFIAIM 172
>SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit
Apc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1458
Score = 29.5 bits (63), Expect = 0.44
Identities = 12/43 (27%), Positives = 24/43 (55%), Gaps = 2/43 (4%)
Frame = -2
Query: 290 VLINHIESLLELCYLSLIKHSKHIGGCTL--CALLVNATTGCL 168
+LIN ++S + LC+L H + + T+ C +V ++ C+
Sbjct: 1119 ILINFLDSFIRLCHLPAKTHDERVTAVTVIRCTQIVALSSSCV 1161
>SPCC1259.11c |gyp2||GTPase activating protein Gyp2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 720
Score = 29.5 bits (63), Expect = 0.44
Identities = 19/53 (35%), Positives = 25/53 (47%), Gaps = 5/53 (9%)
Frame = +1
Query: 394 GP-INFTMFLTLFGERLQG----TDPEDVIKNAFGCFDEXNNGVIGEERLREL 537
GP + F + L LF QG TD V+K CFD N G +E++ L
Sbjct: 404 GPRVLFQVALALFKVNAQGILNATDDSSVMKVFRQCFDHINQGTAADEKMAAL 456
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 29.1 bits (62), Expect = 0.59
Identities = 24/102 (23%), Positives = 39/102 (38%), Gaps = 2/102 (1%)
Frame = +1
Query: 346 KNPTEDYLEGMMNEAP-GPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEXNNGVIG-E 519
K + + +G + P G +N + F ++ + DP + F FD NG I +
Sbjct: 24 KKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFK 83
Query: 520 ERLRELLTTMGDRFTDDDVDEMLREAPIRDGLFDYVEFTRIL 645
E + L T D + +GL Y E RI+
Sbjct: 84 EFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDEMLRIV 125
Score = 28.7 bits (61), Expect = 0.77
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +3
Query: 186 INKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFID 308
+NK Q+ F D + AE+ FN+ D +++G+ID
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEY--VFNVFDADKNGYID 81
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +3
Query: 255 EFKEAFNMIDQNRDGFIDKDDLHDMLASLAQ 347
E +EAF++ D G+ID +DL A L +
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGE 43
>SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 27.1 bits (57), Expect = 2.4
Identities = 12/44 (27%), Positives = 22/44 (50%), Gaps = 1/44 (2%)
Frame = -1
Query: 597 CFPEHFIHIIVCKAIAHCGKQL-AESLLTNHTIVLFIEAPKCIF 469
CF F H I C A+ K + + +T +T++ + +P I+
Sbjct: 137 CFASIFFHAIACYALYLLTKSIFSNQKMTAYTVIFYCFSPSGIY 180
>SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1155
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/46 (26%), Positives = 21/46 (45%)
Frame = +1
Query: 448 TDPEDVIKNAFGCFDEXNNGVIGEERLRELLTTMGDRFTDDDVDEM 585
TD D++KN F F+ +N + L +L + DD + +
Sbjct: 826 TDESDIVKNTFVSFNTTSNSLGNTTALSQLKGHINSVIVDDSYNNI 871
>SPBP23A10.04 |apc2||anaphase-promoting complex subunit Apc2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 681
Score = 25.8 bits (54), Expect = 5.5
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = -1
Query: 486 APKCIFDDILRVSALQSLTKQSEKHRKVNWS 394
A + + D+I VSA+QS + S +V WS
Sbjct: 590 ATETVLDEIQGVSAVQSEAESSAAEMRVYWS 620
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 25.4 bits (53), Expect = 7.2
Identities = 16/55 (29%), Positives = 28/55 (50%)
Frame = -2
Query: 287 LINHIESLLELCYLSLIKHSKHIGGCTLCALLVNATTGCLAR*HGEFYKLAKVNV 123
L+N I++ + + + + HS + C C LLV+A+ G A+ FY N+
Sbjct: 125 LLNLIDTPGHVDFRAEVMHS--LAACEGCILLVDASQGIQAQTLSNFYMAFSQNL 177
>SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 624
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +3
Query: 123 YVYFSKFIKFTMSSRKTAGRRINKKRAQRATSN 221
+VY+S FTMS T I + A AT N
Sbjct: 504 WVYYSNISTFTMSMNDTLSSGILENAALSATQN 536
>SPBC119.14 |rti1||Rad22 homolog Rti1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 371
Score = 25.0 bits (52), Expect = 9.5
Identities = 9/32 (28%), Positives = 17/32 (53%)
Frame = +3
Query: 237 DQAQIAEFKEAFNMIDQNRDGFIDKDDLHDML 332
+ ++ EF+E N + + +DK D HD +
Sbjct: 267 ESPRVEEFEELLNQFEGDEKVSVDKIDAHDKM 298
>SPCC24B10.12 |||CGI121 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 174
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/65 (20%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = -1
Query: 594 FPEHFIHIIVCKAIAHCGKQLAESLLTNHTI--VLFIEAPKCIFDDILRVSALQSLTKQS 421
FPE +H+ V + +++C + E L++ + I F++A ++ + + +++L +
Sbjct: 6 FPETQVHVFVYENVSNCA-AIHEQLISQNPIYDYAFLDAATILYKKQVYSAIIRALEDRR 64
Query: 420 EKHRK 406
++ K
Sbjct: 65 DEQMK 69
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,532,749
Number of Sequences: 5004
Number of extensions: 50049
Number of successful extensions: 157
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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