Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P03_F_A11
(656 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) / putre... 116 1e-26
At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) / putre... 116 1e-26
At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) / putre... 116 2e-26
At5g53120.3 68418.m06603 spermidine synthase, putative / putresc... 109 1e-24
At5g53120.2 68418.m06602 spermidine synthase, putative / putresc... 109 1e-24
At5g53120.1 68418.m06601 spermidine synthase, putative / putresc... 109 1e-24
At5g19530.1 68418.m02326 spermine/spermidine synthase family pro... 81 5e-16
At2g04920.1 68415.m00513 F-box family protein (FBX9) identical t... 31 0.89
At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4... 30 1.6
At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyc... 29 2.7
At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family... 29 2.7
At3g04350.1 68416.m00460 expressed protein 29 2.7
At1g14460.1 68414.m01715 DNA polymerase-related weak similarity ... 28 6.3
>At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 283
Score = 116 bits (279), Expect = 1e-26
Identities = 52/94 (55%), Positives = 68/94 (72%)
Frame = +1
Query: 373 WFTESCDMWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 552
WF+E MWPG S +V++VL KS Y ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 553 QEMISFLPLCCHKNPXNVLIVGGGDGXVAREVAK 654
QEMI+ LPLC NP VL++GGGDG V REVA+
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVAR 140
>At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 334
Score = 116 bits (279), Expect = 1e-26
Identities = 52/94 (55%), Positives = 68/94 (72%)
Frame = +1
Query: 373 WFTESCDMWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 552
WF+E MWPG S +V++VL KS Y ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 553 QEMISFLPLCCHKNPXNVLIVGGGDGXVAREVAK 654
QEMI+ LPLC NP VL++GGGDG V REVA+
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVAR 140
>At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) /
putrescine aminopropyltransferase 2 identical to
SP|O48661 Spermidine synthase 2 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 2) (SPDSY 2)
{Arabidopsis thaliana}
Length = 340
Score = 116 bits (278), Expect = 2e-26
Identities = 51/94 (54%), Positives = 68/94 (72%)
Frame = +1
Query: 373 WFTESCDMWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 552
WF+E MWPG S +V+++L KS Y ++ VF + + GKVLVLDG+IQ T++DE +Y
Sbjct: 51 WFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 110
Query: 553 QEMISFLPLCCHKNPXNVLIVGGGDGXVAREVAK 654
QEMI+ LPLC NP VL++GGGDG V REVA+
Sbjct: 111 QEMITHLPLCSISNPKKVLVIGGGDGGVLREVAR 144
>At5g53120.3 68418.m06603 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 109 bits (263), Expect = 1e-24
Identities = 48/87 (55%), Positives = 66/87 (75%)
Frame = +1
Query: 394 MWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 573
MWPG S +V++VL +KS + + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 574 PLCCHKNPXNVLIVGGGDGXVAREVAK 654
PLC +P NVL+VGGGDG V RE+++
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISR 163
>At5g53120.2 68418.m06602 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 109 bits (263), Expect = 1e-24
Identities = 48/87 (55%), Positives = 66/87 (75%)
Frame = +1
Query: 394 MWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 573
MWPG S +V++VL +KS + + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 574 PLCCHKNPXNVLIVGGGDGXVAREVAK 654
PLC +P NVL+VGGGDG V RE+++
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISR 163
>At5g53120.1 68418.m06601 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 109 bits (263), Expect = 1e-24
Identities = 48/87 (55%), Positives = 66/87 (75%)
Frame = +1
Query: 394 MWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSYQEMISFL 573
MWPG S +V++VL +KS + + VF++ + GKVLVLDGI+Q T+KDE +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 574 PLCCHKNPXNVLIVGGGDGXVAREVAK 654
PLC +P NVL+VGGGDG V RE+++
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISR 163
>At5g19530.1 68418.m02326 spermine/spermidine synthase family
protein similar to SP|P09158 Spermidine synthase (EC
2.5.1.16) (Putrescine aminopropyltransferase)
{Escherichia coli}; contains Pfam profile PF01564:
Spermine/spermidine synthase
Length = 339
Score = 81.4 bits (192), Expect = 5e-16
Identities = 40/95 (42%), Positives = 56/95 (58%)
Frame = +1
Query: 370 NWFTESCDMWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFS 549
+W+ E+ D +SF + VLH S+Y +I + DT GKVLV+DG +Q ++DEF
Sbjct: 34 HWYEETID--DDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFI 91
Query: 550 YQEMISFLPLCCHKNPXNVLIVGGGDGXVAREVAK 654
Y E + L H NP V I+GGG+G ARE+ K
Sbjct: 92 YHECLIHPALLFHPNPKTVFIMGGGEGSAAREILK 126
>At2g04920.1 68415.m00513 F-box family protein (FBX9) identical to
F-box protein family, AtFBX9 (GI:20197985) [Arabidopsis
thaliana]; contains F-box domain PF:00646; contains
TIGRFAM TIGR01640 : F-box protein interaction domain
Length = 376
Score = 30.7 bits (66), Expect = 0.89
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +1
Query: 415 SFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKD 540
S E K VL + S Y + QV+ + KV DG++ CT KD
Sbjct: 75 SIEFKSVLSLKDSHYNSEQVY----IAKVFHCDGLLLCTTKD 112
>At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4)
identical to CBL-interacting protein kinase 4
[Arabidopsis thaliana] gi|13249503|gb|AAG01367;
identical to cDNA calcineurin B-like (CBL) interacting
protein kinase 4 (CIPK4) GI:13249502
Length = 426
Score = 29.9 bits (64), Expect = 1.6
Identities = 18/67 (26%), Positives = 29/67 (43%)
Frame = +1
Query: 367 TNWFTESCDMWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEF 546
T WF +S ++ + FE+ L E I FD SL L L G+ + ++ E
Sbjct: 274 TVWFQKSLEISEFQSSVFELDRFLEKEAKSSNAITAFDLISLSSGLDLSGLFERRKRKEK 333
Query: 547 SYQEMIS 567
+ +S
Sbjct: 334 RFTARVS 340
>At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyclin
3a [Arabidopsis thaliana] GI:509425; contains Pfam
profiles PF00134: Cyclin, N-terminal domain, PF02984:
Cyclin, C-terminal domain; identical to cDNA cyc3b mRNA
for cyclin 3b protein GI:728520
Length = 436
Score = 29.1 bits (62), Expect = 2.7
Identities = 23/85 (27%), Positives = 36/85 (42%), Gaps = 3/85 (3%)
Frame = +1
Query: 82 PQKIYLSTN---RSLSRVSVCHMYRIISLDIYIVIVKCEFSNVEFVRRAKRVFIFRARNT 252
P +YL+ N R LS S R+ L + +++ ++ E F F NT
Sbjct: 224 PDTLYLTVNLIDRFLSN-SYIERQRLQLLGVSCMLIASKYE--ELSAPGVEEFCFITANT 280
Query: 253 LFEREIVKTETXCQNLFNFRLQLPT 327
E++ E N +FRL +PT
Sbjct: 281 YTRPEVLSMEIQILNFVHFRLSVPT 305
>At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 1012
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/68 (25%), Positives = 29/68 (42%)
Frame = +1
Query: 373 WFTESCDMWPGGTFSFEVKEVLHTEKSKYXNIQVFDTTSLGKVLVLDGIIQCTQKDEFSY 552
WF D GG S+ V ++L + F + G VL++ G + F+Y
Sbjct: 369 WFDFMADTGDGGNSSYSVAKLLAQPSLRVPVANNFISLPRGNVLLIGGDLAYPNPSSFTY 428
Query: 553 QEMISFLP 576
++ + F P
Sbjct: 429 EKRL-FCP 435
>At3g04350.1 68416.m00460 expressed protein
Length = 567
Score = 29.1 bits (62), Expect = 2.7
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -3
Query: 537 FLSALYDSVQDKNLPQTGCIKNLD 466
F YD ++ +P GC+KNLD
Sbjct: 227 FFCCTYDLSSERTVPDIGCLKNLD 250
>At1g14460.1 68414.m01715 DNA polymerase-related weak similarity to
DNA polymerase III holoenzyme tau subunit [Thermus
thermophilus] GI:2583049
Length = 1116
Score = 27.9 bits (59), Expect = 6.3
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = -1
Query: 278 VFTISRSNNVFLARNIKTRFALLTNSTLLNSHFT 177
V +I+ S + L RN++ R LL+ + LLNS T
Sbjct: 925 VSSITNSIEMVLRRNVEVRIILLSETELLNSKQT 958
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,255,829
Number of Sequences: 28952
Number of extensions: 263690
Number of successful extensions: 568
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 553
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 568
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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