Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_P19
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 100 4e-22
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 93 2e-20
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 83 3e-17
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 57 2e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 55 1e-08
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 33 0.036
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 29 0.77
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 27 2.4
SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 25 9.5
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 99.5 bits (237), Expect = 4e-22
Identities = 51/134 (38%), Positives = 79/134 (58%)
Frame = +1
Query: 172 FQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQ 351
+++AF LFD G G+I IGD LRA GQNPT +++ + L P E + E FL +
Sbjct: 8 YKQAFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLN 65
Query: 352 AISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGXKLXDDEVXQLLQGXXXS 531
+ +F++G + FDKD G I ELR++L++LG KL ++E+ +LL+G
Sbjct: 66 RPNGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVK 125
Query: 532 XGNINYENFVHLIM 573
G +NY +FV +I+
Sbjct: 126 DGMVNYHDFVQMIL 139
Score = 26.2 bits (55), Expect = 4.1
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +1
Query: 169 EFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDV 282
EF + FQ+FD G I V + L +LG+ + ++
Sbjct: 78 EFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEM 115
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 93.5 bits (222), Expect = 2e-20
Identities = 52/143 (36%), Positives = 81/143 (56%), Gaps = 3/143 (2%)
Frame = +1
Query: 151 SEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDER--IS 324
+++Q+AEF+EAF LFD DG I +G +R+LGQ+PT ++++ + D I
Sbjct: 7 TDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTID 66
Query: 325 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGXKLXDDEVX 504
F FL + K + D + E + FDKDGNG+I+ EL H+L++LG +L +EV
Sbjct: 67 FTEFLTMMAR--KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVA 124
Query: 505 QLL-QGXXXSXGNINYENFVHLI 570
++ + G INYE F +I
Sbjct: 125 DMIREADTDGDGVINYEEFSRVI 147
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 83.4 bits (197), Expect = 3e-17
Identities = 48/142 (33%), Positives = 81/142 (57%), Gaps = 1/142 (0%)
Frame = +1
Query: 151 SEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDERISFE 330
S++Q E +EAF L+D DG I + +G LR+LG N T++++ K + L + I +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEK 61
Query: 331 VFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGXKLXDDEVXQL 510
F+ +K R ++ ++I+ R FDKD +G+I +A+ + TLG KL D+EV +
Sbjct: 62 KFMSFVS--NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLM 119
Query: 511 LQ-GXXXSXGNINYENFVHLIM 573
+Q + G+ +Y +FV IM
Sbjct: 120 VQEADPTNSGSFDYYDFVQRIM 141
Score = 34.7 bits (76), Expect = 0.012
Identities = 16/65 (24%), Positives = 34/65 (52%)
Frame = +1
Query: 169 EFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 348
E+ +AF++FD G I A D ++ LG+ ++++V+ P SF+ + +
Sbjct: 79 EYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQ 138
Query: 349 QAISK 363
+ ++K
Sbjct: 139 RIMAK 143
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 57.2 bits (132), Expect = 2e-09
Identities = 42/143 (29%), Positives = 64/143 (44%)
Frame = +1
Query: 142 AGYSEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDERI 321
A + Q+ E +EAF L D GDG I + L +L Q+ +E + + P I
Sbjct: 40 AQLTSSQIQELKEAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--I 97
Query: 322 SFEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGXKLXDDEV 501
+ FL ++ S ND +E FD +G I + +R LS++G ++ EV
Sbjct: 98 NLAAFLTAMGSMLCRIS--PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEV 155
Query: 502 XQLLQGXXXSXGNINYENFVHLI 570
+L+ S G YE FV I
Sbjct: 156 ESILRS-YTSHGVFYYEKFVDAI 177
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 54.8 bits (126), Expect = 1e-08
Identities = 40/144 (27%), Positives = 63/144 (43%), Gaps = 3/144 (2%)
Frame = +1
Query: 151 SEDQLAEFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDER--IS 324
+E+Q + EAF+LFDS D I + A+RALG N +S+V K + +
Sbjct: 32 TEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQ 91
Query: 325 FEVFLPIYQAISKARSGDTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGXKLXDDEVX 504
E F+ + K D + FD D G IS LR + L + D E+
Sbjct: 92 MEDFVRV--MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELE 149
Query: 505 QLLQG-XXXSXGNINYENFVHLIM 573
+++ G IN + F+ ++M
Sbjct: 150 AMIEEFDLDQDGEINEQEFIAIMM 173
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 33.1 bits (72), Expect = 0.036
Identities = 15/57 (26%), Positives = 26/57 (45%)
Frame = +1
Query: 406 RHFDKDGNGFISSAELRHLLSTLGXKLXDDEVXQLLQGXXXSXGNINYENFVHLIMQ 576
RHF+K + ++ E L++LG +E L S + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 28.7 bits (61), Expect = 0.77
Identities = 22/84 (26%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +1
Query: 328 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGXKLXDDE 498
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G K +
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 499 VXQLLQGXXXSXGNINYENFVHLI 570
+ + + G N EN V ++
Sbjct: 702 LLEHILGLLIETLKRNTENTVAIL 725
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/64 (28%), Positives = 30/64 (46%)
Frame = +1
Query: 169 EFQEAFQLFDSRGDGKIHVAXIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIY 348
E +EAF LFD G I + + LG+N T+ ++ + ++S E F ++
Sbjct: 13 EAEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDLAGTNGKVSREEFAELW 72
Query: 349 QAIS 360
IS
Sbjct: 73 IHIS 76
>SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1131
Score = 25.0 bits (52), Expect = 9.5
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +3
Query: 429 WVHLFCGTATPALY 470
WVHL C + TP +Y
Sbjct: 927 WVHLICASWTPDVY 940
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,354,381
Number of Sequences: 5004
Number of extensions: 43941
Number of successful extensions: 138
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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