Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_P07
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1620.06c |||ribose-phosphate pyrophosphokinase |Schizosaccha... 210 2e-55
SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizo... 166 2e-42
SPBC3D6.06c |||ribose-phosphate pyrophosphokinase |Schizosacchar... 150 1e-37
SPAC630.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 5.5
SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit Git... 25 7.2
SPCC1442.09 |trp3||anthranilate synthase component I|Schizosacch... 25 9.5
>SPCC1620.06c |||ribose-phosphate pyrophosphokinase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 321
Score = 210 bits (512), Expect = 2e-55
Identities = 95/139 (68%), Positives = 119/139 (85%)
Frame = +2
Query: 236 NIKVFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIXXSVRGEDVYIVQSGSGE 415
+IK+F+G+SHP+LA+K+ R+G+ LGKV ++SN ET V I SVR EDV+I+Q+G G
Sbjct: 5 SIKIFAGNSHPELAEKVARRIGLSLGKVAVVQYSNRETSVTIGESVRDEDVFILQTGCGS 64
Query: 416 INDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKXKSRAPITAKLVANILSVSGAD 595
IND+LMELLIMINAC+ ASA R+TA+IPCFPYARQDKK KSRAPITA+LVAN+L +G +
Sbjct: 65 INDHLMELLIMINACRSASARRITAIIPCFPYARQDKKDKSRAPITARLVANMLQTAGCN 124
Query: 596 HIITMDLHASQIQGFFDIP 652
HIITMDLHASQIQGFF++P
Sbjct: 125 HIITMDLHASQIQGFFNVP 143
>SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase
Prs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 166 bits (404), Expect = 2e-42
Identities = 81/141 (57%), Positives = 105/141 (74%)
Frame = +2
Query: 230 MPNIKVFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIXXSVRGEDVYIVQSGS 409
M + + G SHP+L I +RLGI V K+F+N ET VEI SVR +DV+I+QSGS
Sbjct: 1 MKSATIIGGGSHPELLHLISNRLGITPCDVSLKRFANGETSVEIRESVRDKDVFILQSGS 60
Query: 410 GEINDNLMELLIMINACKIASASRVTAVIPCFPYARQDKKXKSRAPITAKLVANILSVSG 589
+ND+LMELLI+I+ACK SA R+TAV+P FPY++Q K K R ITA++VAN+L+V+G
Sbjct: 61 STVNDSLMELLIIISACKGGSAKRITAVMPYFPYSKQSKMRKYRDAITARMVANLLTVAG 120
Query: 590 ADHIITMDLHASQIQGFFDIP 652
DHIIT+DLHASQ+QGFF P
Sbjct: 121 VDHIITLDLHASQMQGFFTRP 141
>SPBC3D6.06c |||ribose-phosphate pyrophosphokinase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 341
Score = 150 bits (364), Expect = 1e-37
Identities = 87/165 (52%), Positives = 105/165 (63%), Gaps = 24/165 (14%)
Frame = +2
Query: 230 MPNIKVFSGSSHPDLAQKIVDRLGIDLGKVVTKKFSNMETCVEIXXSVRGEDVYIVQSGS 409
M N+ VF SHP L + I + L +D+G+V KFSN ET V I SVRG DVYIV S
Sbjct: 1 MKNLVVFGTESHPKLTESICEHLCLDIGRVELSKFSNGETSVRIKQSVRGCDVYIVSPAS 60
Query: 410 GEINDNLMELLIMINACKIASASRVTAVIPCFPYARQ-DKKXK-SRAPI----------- 550
G++ND+LMELLIMI+ACK ASA +VTAV+P FPY+RQ D+K S AP+
Sbjct: 61 GQVNDHLMELLIMISACKTASAKKVTAVLPVFPYSRQPDQKFSFSGAPLSDLQDAVVPCK 120
Query: 551 -----------TAKLVANILSVSGADHIITMDLHASQIQGFFDIP 652
+ LVA++L SGADHIITMDLH Q QGFFDIP
Sbjct: 121 KQTGYHPWIAQSGTLVADLLMCSGADHIITMDLHDPQFQGFFDIP 165
>SPAC630.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 27.5 bits (58), Expect = 1.8
Identities = 14/42 (33%), Positives = 20/42 (47%)
Frame = +2
Query: 497 PCFPYARQDKKXKSRAPITAKLVANILSVSGADHIITMDLHA 622
PCF Y + K+ K I + V N S G + +DLH+
Sbjct: 93 PCFSYCARLKEPKDLLEIGSVSVDNKCSTCGLFRVSRIDLHS 134
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/39 (33%), Positives = 19/39 (48%), Gaps = 1/39 (2%)
Frame = +3
Query: 522 TKXTRAEPRSRPNSLPTSF-QCLEPITSSLWIFMRRKFK 635
T+ R PR P ++ +EP TS +W+F K K
Sbjct: 76 TQYQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNK 114
>SPBC32H8.07 |git5|gpb1|heterotrimeric G protein beta subunit
Git5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 305
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +2
Query: 572 ILSVSGADHIITMDLHASQIQGFFDIP 652
+LS S D ++ DLH S FF+ P
Sbjct: 36 LLSASTNDVLLVWDLHTSNKVAFFEAP 62
>SPCC1442.09 |trp3||anthranilate synthase component
I|Schizosaccharomyces pombe|chr 3|||Manual
Length = 489
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +2
Query: 338 NMETCVEIXXSVRGEDVYIVQSGSGEINDN 427
NM+TC+ I V + +Q+G G + D+
Sbjct: 425 NMDTCIAIRTMVYKDGTVYLQAGGGIVFDS 454
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,396,492
Number of Sequences: 5004
Number of extensions: 43210
Number of successful extensions: 129
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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