BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_M03 (600 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_21409| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.058 SB_59206| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 0.54 >SB_21409| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 195 Score = 34.7 bits (76), Expect = 0.058 Identities = 39/144 (27%), Positives = 58/144 (40%), Gaps = 13/144 (9%) Frame = +2 Query: 203 DIXIEGFNPSAEEADEGTDSAVESGVDIVLNHRLVETYAFGDKKSYTXXXXXXXXXXXXX 382 D + G N SAE+A + + +G ++V+ +RLVE K+ Sbjct: 47 DENLIGGNKSAEDACDDVEDGCTTGCNVVMANRLVEIPYKTFKELLAEFKPYIKKVKEHM 106 Query: 383 XEXKAPD-QVEVFKTNMNKVMKDILGRFKELQFFTGESMDCD-----GMVAMMEY----- 529 E PD +V+ F+ + I FKE QFF DCD + M Y Sbjct: 107 KEEGCPDEEVKGFEKAAMDYILSIKKNFKEYQFF---QPDCDLGGGAHCIVFMWYPEKDL 163 Query: 530 --XDFDGTQIPIMMFFKHGLQXEK 595 D DG P++ FKH ++ K Sbjct: 164 RGVDQDGFS-PVLTVFKHAVKQVK 186 >SB_59206| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 499 Score = 31.5 bits (68), Expect = 0.54 Identities = 18/50 (36%), Positives = 30/50 (60%) Frame = -2 Query: 200 PCALVTNLPVTS*ITSSTNFIL*VSENISSASDNVLIDLHFDGLEAIKNN 51 PC L+ L T T+ +L +E++SSA+ + + D+ FDG++ I NN Sbjct: 106 PCLLLLTLVFT------TSQVLTTAEDVSSATIDFVGDISFDGIQKIFNN 149 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,464,784 Number of Sequences: 59808 Number of extensions: 294171 Number of successful extensions: 501 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 476 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 501 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1451595000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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