Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_L17
(651 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 161 6e-41
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 93 2e-20
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 87 2e-18
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 87 2e-18
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 77 3e-15
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 75 1e-14
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 60 2e-10
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 29 0.44
SPAC343.17c |||WD repeat protein, human WDR70 family|Schizosacch... 25 7.2
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 161 bits (392), Expect = 6e-41
Identities = 74/136 (54%), Positives = 101/136 (74%)
Frame = +1
Query: 214 SAGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQR 393
+AG+TSV + G + A V +Q+KVPD+L+D +V H+F +T IGCVMTG IAD+R+QV R
Sbjct: 32 NAGITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVSR 91
Query: 394 ARYEAANWQYKYGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLIAYDDEAGPCVYK 573
AR EAA ++YK G +P +L +R+A+I+QV TQ A MRPLG +M L+A DDE GP ++K
Sbjct: 92 ARSEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMTLVAVDDEIGPSLFK 151
Query: 574 TDPSGYYCSYKAVXAG 621
DP+G+Y YKA AG
Sbjct: 152 LDPAGFYIGYKATSAG 167
Score = 62.1 bits (144), Expect = 7e-11
Identities = 28/50 (56%), Positives = 33/50 (66%)
Frame = +3
Query: 135 GFDRHITIFSPEGRLYQVEYALKAINQRWVDICGITRYRCSCSGSSTQGP 284
GFDR IT+FSPEGRLYQVEYA KA N + G+T C+C S + P
Sbjct: 6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVP 55
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 93.5 bits (222), Expect = 2e-20
Identities = 55/148 (37%), Positives = 81/148 (54%), Gaps = 2/148 (1%)
Frame = +1
Query: 214 SAGLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQR 393
+AG+TSV ++ TD V+A ++K L S+ + A+T IG V +GM D R V +
Sbjct: 29 NAGVTSVGIKATDGVVLATEKKPTSELAIGASLEKVCAITPDIGMVYSGMGPDFRVLVDK 88
Query: 394 ARYEAANWQYK--YGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLIAYDDEAGPCV 567
+R + A YK Y P IL + IA + Q TQ+ +RP G S+ L+A DE GP +
Sbjct: 89 SR-KVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGGVRPFGVSL-LVAGMDEKGPSL 146
Query: 568 YKTDPSGYYCSYKAVXAGAXTVDGNAYL 651
Y+ DPSG Y ++KA G + +L
Sbjct: 147 YQVDPSGTYFAWKATAIGKSSTAAKTFL 174
Score = 34.3 bits (75), Expect = 0.015
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = +3
Query: 150 ITIFSPEGRLYQVEYALKAIN 212
+T FSP G+L Q+EYAL A+N
Sbjct: 9 LTTFSPNGKLVQIEYALNAVN 29
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 87.0 bits (206), Expect = 2e-18
Identities = 48/146 (32%), Positives = 78/146 (53%), Gaps = 2/146 (1%)
Frame = +1
Query: 220 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCV-MTGMIADSRSQVQRA 396
G T++ALRG + V+ +RK +L + VS A+ D C+ G+ AD+R + +A
Sbjct: 30 GTTAIALRGNECIVIGVERKNVPKLQN-VSNFQKIAMVDNHVCLAFAGLNADARILIDKA 88
Query: 397 RYEAANWQYKYGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLIAYD-DEAGPCVYK 573
R EA N + + + L R +A + Q YTQ+ +RP G S ++ +D + P VY+
Sbjct: 89 RVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFDVGDNTPRVYQ 148
Query: 574 TDPSGYYCSYKAVXAGAXTVDGNAYL 651
T+P+G Y ++KA G + YL
Sbjct: 149 TEPAGIYNAWKATAIGRASKAAREYL 174
Score = 38.7 bits (86), Expect = 7e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +3
Query: 132 AGFDRHITIFSPEGRLYQVEYALKAINQ 215
+G+DR +++FSP+GRL QVEY +A+ +
Sbjct: 2 SGYDRALSVFSPDGRLLQVEYGQEAVRR 29
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 87.0 bits (206), Expect = 2e-18
Identities = 43/132 (32%), Positives = 70/132 (53%)
Frame = +1
Query: 226 TSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRARYE 405
T + ++ D ++A ++ V +LL P + ++ IG TG I D + V+RAR E
Sbjct: 35 TCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRARDE 94
Query: 406 AANWQYKYGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLIAYDDEAGPCVYKTDPS 585
A +W+ YGS IP ++ R+ + Q++T + +RP G + YD E GP +Y +P+
Sbjct: 95 ATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATYDSE-GPHLYMVEPN 153
Query: 586 GYYCSYKAVXAG 621
G Y Y AG
Sbjct: 154 GVYWGYNGAAAG 165
Score = 32.7 bits (71), Expect = 0.047
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +3
Query: 135 GFDRHITIFSPEGRLYQVEYALKAI 209
G+D + FSP+GRL+Q EYA KA+
Sbjct: 7 GYDLGL-FFSPDGRLFQAEYAYKAV 30
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 76.6 bits (180), Expect = 3e-15
Identities = 41/141 (29%), Positives = 76/141 (53%), Gaps = 6/141 (4%)
Frame = +1
Query: 220 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRAR 399
G T++ ++ DA V+ ++++ L++ SV LF + IGC ++G+ AD+R+ ++ AR
Sbjct: 34 GSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEHAR 93
Query: 400 YEAANWQYKYGSXIPVHILCRRIADISQVY------TQNAXMRPLGCSMMLIAYDDEAGP 561
+ N ++ Y + + I D++ + + RP G + +LIA DE GP
Sbjct: 94 VQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVA-LLIAGIDEHGP 152
Query: 562 CVYKTDPSGYYCSYKAVXAGA 624
+Y ++PSG Y Y+A G+
Sbjct: 153 QLYHSEPSGTYFRYEAKAIGS 173
Score = 40.3 bits (90), Expect = 2e-04
Identities = 16/24 (66%), Positives = 21/24 (87%)
Frame = +3
Query: 138 FDRHITIFSPEGRLYQVEYALKAI 209
+DR + FSPEGRL+QVEYA++AI
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAI 31
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 74.5 bits (175), Expect = 1e-14
Identities = 39/125 (31%), Positives = 66/125 (52%), Gaps = 1/125 (0%)
Frame = +1
Query: 250 DAAVVAAQRKVPDRLLDPV-SVTHLFALTDRIGCVMTGMIADSRSQVQRARYEAANWQYK 426
D V+AA++KV +LL+ S L+ + D + C + G+ AD+ + AR +
Sbjct: 41 DGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQYLQT 100
Query: 427 YGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLIAYDDEAGPCVYKTDPSGYYCSYK 606
+ +P L RR+ D+ Q YTQ +RP G S + +D G +++++PSG Y S++
Sbjct: 101 FNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQ 160
Query: 607 AVXAG 621
A G
Sbjct: 161 ANSIG 165
Score = 47.6 bits (108), Expect = 2e-06
Identities = 22/32 (68%), Positives = 24/32 (75%)
Frame = +3
Query: 129 SAGFDRHITIFSPEGRLYQVEYALKAINQRWV 224
S +D TIFSPEGRLYQVEYAL+AIN V
Sbjct: 2 SRSYDSRTTIFSPEGRLYQVEYALEAINHAGV 33
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 60.5 bits (140), Expect = 2e-10
Identities = 39/144 (27%), Positives = 65/144 (45%)
Frame = +1
Query: 220 GLTSVALRGTDAAVVAAQRKVPDRLLDPVSVTHLFALTDRIGCVMTGMIADSRSQVQRAR 399
G +V L AV+ A ++ + L L + D IG + G+ D+R +
Sbjct: 32 GSATVGLVSKTHAVLVALKRNAEELSSYQK--KLIRIDDHIGIAIAGLAPDARVLSNYMK 89
Query: 400 YEAANWQYKYGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLIAYDDEAGPCVYKTD 579
EA + + + IPV L ++A+ +Q+ TQ RP G ++I Y DE+GP + +
Sbjct: 90 QEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIGY-DESGPHLLEFQ 148
Query: 580 PSGYYCSYKAVXAGAXTVDGNAYL 651
PSG Y G+ + Y+
Sbjct: 149 PSGLVLEYLGTSMGSRSQSARTYI 172
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/26 (61%), Positives = 21/26 (80%)
Frame = +3
Query: 138 FDRHITIFSPEGRLYQVEYALKAINQ 215
+D T +SP+GRL+QVEYAL+AI Q
Sbjct: 6 YDGDATTWSPQGRLHQVEYALEAIKQ 31
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 29.5 bits (63), Expect = 0.44
Identities = 15/62 (24%), Positives = 25/62 (40%)
Frame = +1
Query: 358 GMIADSRSQVQRARYEAANWQYKYGSXIPVHILCRRIADISQVYTQNAXMRPLGCSMMLI 537
GM +R+ Q +E +W Y + V LCR + ++ N + + LI
Sbjct: 606 GMCPYTRAVSQHVEWENDSWMYVLNVSLQVAKLCRHVGNVFMELNTNKLANAINYLISLI 665
Query: 538 AY 543
Y
Sbjct: 666 LY 667
>SPAC343.17c |||WD repeat protein, human WDR70
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 576
Score = 25.4 bits (53), Expect = 7.2
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -3
Query: 412 WQPRSELVVLETGYQQSSR 356
WQP S + L TGY ++R
Sbjct: 205 WQPDSSQIFLTTGYDSTAR 223
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,445,299
Number of Sequences: 5004
Number of extensions: 44063
Number of successful extensions: 106
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 102
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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