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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P02_F_K21
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g67270.1 68414.m07656 hypothetical protein                          29   2.0  
At3g58820.1 68416.m06556 F-box family protein contains F-box dom...    29   2.7  
At1g16110.1 68414.m01931 wall-associated kinase, putative contai...    28   4.7  
At5g43090.1 68418.m05260 pumilio/Puf RNA-binding domain-containi...    27   8.2  

>At1g67270.1 68414.m07656 hypothetical protein
          Length = 506

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 13/40 (32%), Positives = 19/40 (47%)
 Frame = +3

Query: 390 YDTFRGHSHYKCYPQKLKNGDPFYMFTYHSICCIKYCTKC 509
           YD+  G S ++C  + L    P        +C IK+C KC
Sbjct: 19  YDSQNGKSCHQCRQKTLDFAAPCKAMRRKKLCPIKFCYKC 58


>At3g58820.1 68416.m06556 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 413

 Score = 29.1 bits (62), Expect = 2.7
 Identities = 15/66 (22%), Positives = 33/66 (50%)
 Frame = +1

Query: 358 RGFQNIPITVVMTPLGDTAIINAILKSLKMETHSTCLRITQYVVSSTVQNVPCMFRDLKN 537
           RG+Q IP+ +   P  +T +I  +L ++  +    C  I++     ++ + P    +++ 
Sbjct: 273 RGWQAIPVLLRNCPHLETLVIEGVLHNVTDKCGDACDCISREDKGRSLTSCPVKMLEIRG 332

Query: 538 FAHTVK 555
           F  T+K
Sbjct: 333 FRGTMK 338


>At1g16110.1 68414.m01931 wall-associated kinase, putative contains
           similarity to wall-associated kinase 2 GI:4826399 from
           [Arabidopsis thaliana]
          Length = 642

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 14/40 (35%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
 Frame = +3

Query: 342 NLLCYAWFSKYTHYSC-YDTFRGHSHYKCYPQKLKNGDPF 458
           N+  Y+ +S +   SC YD F G S+  CY      G+P+
Sbjct: 276 NMTRYSSYSSFDKCSCEYDYFSGMSYRICYCNYGYTGNPY 315


>At5g43090.1 68418.m05260 pumilio/Puf RNA-binding domain-containing
           protein contains similarity to RNA-binding protein
          Length = 527

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 30/112 (26%), Positives = 47/112 (41%), Gaps = 4/112 (3%)
 Frame = +1

Query: 301 HCELFYKSADGPNETCFAMRGF--QNIPITVVMTPLGDTAIINAILKSLKMETHSTCLRI 474
           HC   Y+ A   N  C   + F  + +P   +   L    I +A+   L +  H     +
Sbjct: 362 HC---YQIAIDQNGCCLLQQCFDKERVPNHEIRQRLISEVIEHAL--KLCLNCHGNY--V 414

Query: 475 TQYVVSSTVQNVPCMFRD--LKNFAHTVKNNIISPVKSSILKYYGYPSASLV 624
            QYVV    Q+   +  +  L+N+AH  +N   S V   +LK  G  S  +V
Sbjct: 415 VQYVVELDNQHETDLLVNKLLRNYAHLARNKYGSHVVQKLLKLRGIDSKLIV 466


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,645,603
Number of Sequences: 28952
Number of extensions: 246063
Number of successful extensions: 531
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 519
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 531
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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