Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P02_F_J16
         (631 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g03110.1 68416.m00307 exportin 1, putative strong similarity ...   120   6e-28
At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to Ex...   119   1e-27
At3g04490.1 68416.m00476 exportin-related weak similarity to Exp...    29   2.5  
At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2 (R...    28   5.9  

>At3g03110.1 68416.m00307 exportin 1, putative strong similarity to
           Exportin1 (XPO1) protein [Arabidopsis thaliana]
           GI:7671510; contains Pfam profile PF03810: Importin-beta
           N-terminal domain
          Length = 1076

 Score =  120 bits (290), Expect = 6e-28
 Identities = 59/155 (38%), Positives = 91/155 (58%), Gaps = 1/155 (0%)
 Frame = +1

Query: 169 ASKLLDFNQKLDINLLDNIVGCLYSAVGDQQRVAQD-ILTALKEHPDAWTRVDTILEYSQ 345
           A KL D +Q +D+ LLD  V   YS    ++R + D IL  LK +PD W +V  IL+ + 
Sbjct: 3   AEKLRDLSQPIDVVLLDATVEAFYSTGSKEERASADNILRDLKANPDTWLQVVHILQNTS 62

Query: 346 NQETKYYALQILXQVILTXWKILPXNQCXXXXXXXXXXXXXNSSXPVTMESNKVYLKKLN 525
           +  TK++ALQ+L  VI   W  LP  Q               S    +  + ++Y+ KLN
Sbjct: 63  STHTKFFALQVLEGVIKYRWNALPVEQRDGMKNYISDVIVQLSRDEASFRTERLYVNKLN 122

Query: 526 MILIQVLKREWPHNWETFISDIVGASKTNESLCQN 630
           +IL+Q++K+EWP  W++FI D+V A+KT+E++C+N
Sbjct: 123 IILVQIVKQEWPAKWKSFIPDLVIAAKTSETICEN 157


>At5g17020.1 68418.m01995 exportin1 (XPO1) nearly identical to
           Exportin1 (XPO1) protein [Arabidopsis thaliana]
           GI:7671510
          Length = 1075

 Score =  119 bits (287), Expect = 1e-27
 Identities = 57/155 (36%), Positives = 90/155 (58%), Gaps = 1/155 (0%)
 Frame = +1

Query: 169 ASKLLDFNQKLDINLLDNIVGCLYSAVGDQQRVAQD-ILTALKEHPDAWTRVDTILEYSQ 345
           A KL D +Q +D+ +LD  V   +     ++R A D IL  L+ +PD W +V  IL+ + 
Sbjct: 3   AEKLRDLSQPIDVGVLDATVAAFFVTGSKEERAAADQILRDLQANPDMWLQVVHILQNTN 62

Query: 346 NQETKYYALQILXQVILTXWKILPXNQCXXXXXXXXXXXXXNSSXPVTMESNKVYLKKLN 525
           + +TK++ALQ+L  VI   W  LP  Q               SS   +  S ++Y+ KLN
Sbjct: 63  SLDTKFFALQVLEGVIKYRWNALPVEQRDGMKNYISEVIVQLSSNEASFRSERLYVNKLN 122

Query: 526 MILIQVLKREWPHNWETFISDIVGASKTNESLCQN 630
           +IL+Q++K +WP  W +FI D+V A+KT+E++C+N
Sbjct: 123 VILVQIVKHDWPAKWTSFIPDLVAAAKTSETICEN 157


>At3g04490.1 68416.m00476 exportin-related weak similarity to
           Exportin 4 (Exp4) (Swiss-Prot:Q9ESJ0) [Mus musculus] and
           (Swiss-Prot:Q9C0E2) [Homo sapiens]
          Length = 921

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 20/111 (18%), Positives = 41/111 (36%)
 Frame = +1

Query: 226 VGCLYSAVGDQQRVAQDILTALKEHPDAWTRVDTILEYSQNQETKYYALQILXQVILTXW 405
           + C Y  +      A+  + +L + P  +     ILE SQ    ++ A   + +  +  W
Sbjct: 30  LACSYIQINSNPVAAEATILSLHQSPQPYKACRYILENSQVANARFQAAAAIRESAIREW 89

Query: 406 KILPXNQCXXXXXXXXXXXXXNSSXPVTMESNKVYLKKLNMILIQVLKREW 558
             L  +               +++      S    L K++ +  Q++KR W
Sbjct: 90  SFLATDDKGGLISFCLGYVMQHAN-----SSEGYVLSKVSSVAAQLMKRGW 135


>At2g20580.1 68415.m02404 26S proteasome regulatory subunit S2
           (RPN1) contains an APC-complex (cyclosome) and
           proteasome component repeat ( PS50248)
          Length = 891

 Score = 27.9 bits (59), Expect = 5.9
 Identities = 15/56 (26%), Positives = 27/56 (48%)
 Frame = +1

Query: 133 IYFTRMATLEQQASKLLDFNQKLDINLLDNIVGCLYSAVGDQQRVAQDILTALKEH 300
           +Y  +  ++E  A     FN+K+       ++ C Y+  G+  +V QD+L    EH
Sbjct: 570 LYLGKQESVEATAEVSKTFNEKIRKYCDMTLLSCAYAGTGNVLKV-QDLLAQCGEH 624


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,875,545
Number of Sequences: 28952
Number of extensions: 214418
Number of successful extensions: 484
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 477
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 482
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)