Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_J08
(651 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.06 |gln1||glutamate-ammonia ligase Gln1|Schizosaccharom... 32 0.083
SPBC887.14c |pfh1|pif1|pif1 helicase homolog Pfh1|Schizosaccharo... 27 1.8
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces... 27 2.3
SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.1
SPCC18.02 |||membrane transporter|Schizosaccharomyces pombe|chr ... 26 4.1
SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces... 26 4.1
SPAC6B12.14c |||conserved fungal protein|Schizosaccharomyces pom... 26 4.1
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 26 5.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 25 7.2
SPCC594.01 ||SPCC736.16|DUF1769 family protein|Schizosaccharomyc... 25 7.2
SPBC2D10.04 |||arrestin Aly1 related|Schizosaccharomyces pombe|c... 25 9.5
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 25 9.5
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 25 9.5
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa... 25 9.5
>SPAC23H4.06 |gln1||glutamate-ammonia ligase
Gln1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 359
Score = 31.9 bits (69), Expect = 0.083
Identities = 16/33 (48%), Positives = 20/33 (60%)
Frame = -3
Query: 562 RLTCSRF*LRMAAEDFGVEVLVQPKAGPVLDGW 464
+L SRF L AEDFGV++ PK P+L W
Sbjct: 218 QLWMSRFLLHRIAEDFGVKISFHPK--PILGDW 248
>SPBC887.14c |pfh1|pif1|pif1 helicase homolog
Pfh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 805
Score = 27.5 bits (58), Expect = 1.8
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 4/65 (6%)
Frame = +2
Query: 260 PYPQRQPQSHQNSQHVPTNQDSTRRHHHQASQTDSTT---TGNDLSCYHLNKRLST-LSS 427
P P+R S SQH +N S + + ++S DS S ++K+ S L+S
Sbjct: 225 PDPKRTSTSADISQHTVSNDSSNKLSNGRSSSLDSLAKKRMSKSKSTPQISKKFSVPLNS 284
Query: 428 DRKSP 442
KSP
Sbjct: 285 ASKSP 289
>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 609
Score = 27.1 bits (57), Expect = 2.3
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = +1
Query: 445 TEASLLSIHQEQVQPLVEPRPQH 513
TE +L HQE QPL++ QH
Sbjct: 530 TEPQILEYHQEWPQPLIQSIQQH 552
>SPCC553.10 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 349
Score = 26.6 bits (56), Expect = 3.1
Identities = 24/94 (25%), Positives = 35/94 (37%), Gaps = 3/94 (3%)
Frame = +2
Query: 212 TSRTRWPSKWTM*LHHPYPQRQPQSHQNSQH---VPTNQDSTRRHHHQASQTDSTTTGND 382
T+ T WP+ ++ YP R Q + SQ V S+ +S S+TT +
Sbjct: 77 TNTTNWPTGQGFQINMAYPGRPEQIYAQSQQFNIVEGAASSSSSSSSSSSSLVSSTTSSS 136
Query: 383 LSCYHLNKRLSTLSSDRKSPRQKPHCFPSIKNRS 484
S S+ SS S S K+ S
Sbjct: 137 SSATPSTTSSSSSSSSSSSSSSSKSSSSSSKSSS 170
>SPCC18.02 |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 448
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 382 FILLPLEQATQYTVIRPQEPKTEASLLSIHQEQV 483
FI+L L+ A ++ +I P+E K +++L + + +
Sbjct: 182 FIILLLDIALRFLMIEPREMKNDSTLQDVERTSI 215
>SPAC2F3.09 |hem1||5-aminolevulinate synthase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 558
Score = 26.2 bits (55), Expect = 4.1
Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 2/50 (4%)
Frame = +3
Query: 132 SNFGARSQTASSAFRLLPPGGHADHSSPHGP--DGLRSGRCNSIIHIHND 275
S + A T S+ R LP H +G+R+ RC II HND
Sbjct: 242 SCYVANDATLSTLGRKLPNCIFLSDEMNHASMINGIRNSRCEKIIFKHND 291
>SPAC6B12.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 154
Score = 26.2 bits (55), Expect = 4.1
Identities = 19/71 (26%), Positives = 30/71 (42%)
Frame = +3
Query: 150 SQTASSAFRLLPPGGHADHSSPHGPDGLRSGRCNSIIHIHNDNHKATRTHNTSPQTRILR 329
+Q L+ G D S + + L S +S +H H+DN R +T Q I+
Sbjct: 55 NQALREVLELIESGASIDFISQYCLEKLASSDNDSSVHNHHDN-SVLRNEDTQLQPHIV- 112
Query: 330 AVITTKRHRPI 362
T + RP+
Sbjct: 113 CNRTVRAGRPV 123
>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 732
Score = 25.8 bits (54), Expect = 5.4
Identities = 14/45 (31%), Positives = 20/45 (44%)
Frame = +2
Query: 296 SQHVPTNQDSTRRHHHQASQTDSTTTGNDLSCYHLNKRLSTLSSD 430
SQH T+++S HH D N+ C H + L L +D
Sbjct: 528 SQHEHTHENSQEHHHEHNHNHDHIHKYNE-KCDHESISLQNLDND 571
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.4 bits (53), Expect = 7.2
Identities = 8/28 (28%), Positives = 14/28 (50%)
Frame = +2
Query: 251 LHHPYPQRQPQSHQNSQHVPTNQDSTRR 334
+H PY +P+S Q P+ +D +
Sbjct: 376 MHSPYSTHEPESEPEDQDEPSEKDDENK 403
>SPCC594.01 ||SPCC736.16|DUF1769 family protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 791
Score = 25.4 bits (53), Expect = 7.2
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +2
Query: 323 STRRHHHQASQTDSTTTGNDLSCYHLNKRLSTLSS 427
S RHHH +TD + + + N+R S + S
Sbjct: 477 SLHRHHHHHHKTDLIESDSGIEASESNRRKSDIFS 511
>SPBC2D10.04 |||arrestin Aly1 related|Schizosaccharomyces pombe|chr
2|||Manual
Length = 658
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/42 (30%), Positives = 18/42 (42%)
Frame = +2
Query: 215 SRTRWPSKWTM*LHHPYPQRQPQSHQNSQHVPTNQDSTRRHH 340
SRT WP + H Y + SH + PT +D+ H
Sbjct: 201 SRTEWPEGIPVKGHDTYEDKVIISHNWKFYEPTMKDADAPQH 242
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 25.0 bits (52), Expect = 9.5
Identities = 17/64 (26%), Positives = 25/64 (39%), Gaps = 1/64 (1%)
Frame = +2
Query: 266 PQRQPQSHQNSQHVPTNQDSTRRHHHQASQTDSTTTGNDLSCYHLNKRLSTLSSDR-KSP 442
P R +S + Q + T H S T TTT + STL++ R +P
Sbjct: 152 PTRSTRSSSHIQSLSPESKQTSDGHRPPSPTSITTTSTSIDPSVAFSSKSTLATTRTNAP 211
Query: 443 RQKP 454
+P
Sbjct: 212 LSRP 215
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.0 bits (52), Expect = 9.5
Identities = 13/47 (27%), Positives = 23/47 (48%)
Frame = +3
Query: 111 NIPDN*RSNFGARSQTASSAFRLLPPGGHADHSSPHGPDGLRSGRCN 251
N+P + + A+SQ+ + L + S P GP G+++G N
Sbjct: 236 NLPVHPATAINAQSQSGNPLSNPLFQPSNVPQSIPSGPMGMKTGSVN 282
>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 783
Score = 25.0 bits (52), Expect = 9.5
Identities = 15/53 (28%), Positives = 27/53 (50%)
Frame = +3
Query: 264 IHNDNHKATRTHNTSPQTRILRAVITTKRHRPIQPLLATIYPATT*TSDSVHC 422
I ++ H+AT H+ + +++RAV+ + H + L AT +T V C
Sbjct: 232 IFDEAHRATGNHSYA---QVMRAVLRSNSHFRVLGLTATPGSSTASVQKVVDC 281
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,579,685
Number of Sequences: 5004
Number of extensions: 53203
Number of successful extensions: 188
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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