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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P02_F_J01
         (654 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    35   0.012
SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc...    33   0.027
SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |...    27   1.8  
SPBC3E7.05c |||conserved eukaryotic protein|Schizosaccharomyces ...    27   2.4  
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|...    25   7.2  
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    25   9.5  

>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 34.7 bits (76), Expect = 0.012
 Identities = 26/75 (34%), Positives = 37/75 (49%), Gaps = 4/75 (5%)
 Frame = -1

Query: 387 LCSLHKTGKSF----MACLRIATSSSSHKSNWASIACSDKVGKLLKNFAMN*DAKRFSLS 220
           L +L KT +S     M+CLRI  +SSS    W++     +V   LKN   N  A  F +S
Sbjct: 254 LLNLVKTSESLSSQQMSCLRICCNSSSF---WSAADSFKEVSSFLKNLLKNVTATPFEVS 310

Query: 219 DPFTAASISKYSFSC 175
           D   A  I+ +  +C
Sbjct: 311 DMNWAYIIASFFRTC 325


>SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 488

 Score = 33.5 bits (73), Expect = 0.027
 Identities = 34/125 (27%), Positives = 55/125 (44%), Gaps = 9/125 (7%)
 Frame = +2

Query: 146 LAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQ-FDLCE 322
           L   K E+S         L  +    KE+  A + +AKF ++  T+S + IE Q  ++ E
Sbjct: 94  LTETKQELSTALYSLDAALRVISRLTKERDEAREALAKFSDNIGTVSSKTIEVQEVEMGE 153

Query: 323 DDD---VAIRKQAIKDLPVLCKEHKXHTQR----IADILAQLLQSEDSTEI-NVVTNSLV 478
            DD    ++R    K    L  + K    +      D ++QLLQ+  ST + N+ T S  
Sbjct: 154 SDDQLKTSLRSTVEKTFQELSSKRKRTKLQPKWATDDAVSQLLQATPSTILENLETESTT 213

Query: 479 TILKS 493
           +   S
Sbjct: 214 SFFPS 218


>SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 661

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 2/51 (3%)
 Frame = +2

Query: 164 EISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFP--TLSEQAIEAQF 310
           +I + E+E+LEILA ++  +KE    S+  A    + P   LSE+ +++Q+
Sbjct: 179 DIVRSEEEFLEILAKLEQQEKEASNVSE--ASRIATIPPMILSEEEVKSQY 227


>SPBC3E7.05c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 550

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 12/44 (27%), Positives = 24/44 (54%)
 Frame = +2

Query: 182 KEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQFD 313
           ++++  L      D+   LAS+ I K  +S P L ++ ++ +FD
Sbjct: 394 RQHVNTLKEATKDDELAALASRVIEKIVDSGPILDKEELQTKFD 437


>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 732

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 10/25 (40%), Positives = 16/25 (64%)
 Frame = -3

Query: 364 QIFYGLFTNSNVVILAQVKLGFYCL 290
           Q+ YGL+TNS  +I   + + F C+
Sbjct: 364 QVIYGLWTNSLGLISDAIHMAFDCI 388


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = +2

Query: 398 QRIADILAQLLQSEDSTEINVVTNSLVTILKSDPK 502
           +R+  + A LL  E+   + ++TNSL   LKS  K
Sbjct: 110 KRLGYLAAMLLLDENQEVLTLLTNSLQNDLKSRDK 144


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,476,805
Number of Sequences: 5004
Number of extensions: 48708
Number of successful extensions: 135
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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