BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_J01 (654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 35 0.012 SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosacc... 33 0.027 SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |... 27 1.8 SPBC3E7.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 2.4 SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 25 7.2 SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 25 9.5 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 34.7 bits (76), Expect = 0.012 Identities = 26/75 (34%), Positives = 37/75 (49%), Gaps = 4/75 (5%) Frame = -1 Query: 387 LCSLHKTGKSF----MACLRIATSSSSHKSNWASIACSDKVGKLLKNFAMN*DAKRFSLS 220 L +L KT +S M+CLRI +SSS W++ +V LKN N A F +S Sbjct: 254 LLNLVKTSESLSSQQMSCLRICCNSSSF---WSAADSFKEVSSFLKNLLKNVTATPFEVS 310 Query: 219 DPFTAASISKYSFSC 175 D A I+ + +C Sbjct: 311 DMNWAYIIASFFRTC 325 >SPAC29A4.08c |prp19|cwf8|ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 33.5 bits (73), Expect = 0.027 Identities = 34/125 (27%), Positives = 55/125 (44%), Gaps = 9/125 (7%) Frame = +2 Query: 146 LAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQ-FDLCE 322 L K E+S L + KE+ A + +AKF ++ T+S + IE Q ++ E Sbjct: 94 LTETKQELSTALYSLDAALRVISRLTKERDEAREALAKFSDNIGTVSSKTIEVQEVEMGE 153 Query: 323 DDD---VAIRKQAIKDLPVLCKEHKXHTQR----IADILAQLLQSEDSTEI-NVVTNSLV 478 DD ++R K L + K + D ++QLLQ+ ST + N+ T S Sbjct: 154 SDDQLKTSLRSTVEKTFQELSSKRKRTKLQPKWATDDAVSQLLQATPSTILENLETESTT 213 Query: 479 TILKS 493 + S Sbjct: 214 SFFPS 218 >SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 661 Score = 27.5 bits (58), Expect = 1.8 Identities = 17/51 (33%), Positives = 31/51 (60%), Gaps = 2/51 (3%) Frame = +2 Query: 164 EISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFP--TLSEQAIEAQF 310 +I + E+E+LEILA ++ +KE S+ A + P LSE+ +++Q+ Sbjct: 179 DIVRSEEEFLEILAKLEQQEKEASNVSE--ASRIATIPPMILSEEEVKSQY 227 >SPBC3E7.05c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 27.1 bits (57), Expect = 2.4 Identities = 12/44 (27%), Positives = 24/44 (54%) Frame = +2 Query: 182 KEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQFD 313 ++++ L D+ LAS+ I K +S P L ++ ++ +FD Sbjct: 394 RQHVNTLKEATKDDELAALASRVIEKIVDSGPILDKEELQTKFD 437 >SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 25.4 bits (53), Expect = 7.2 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = -3 Query: 364 QIFYGLFTNSNVVILAQVKLGFYCL 290 Q+ YGL+TNS +I + + F C+ Sbjct: 364 QVIYGLWTNSLGLISDAIHMAFDCI 388 >SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 865 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +2 Query: 398 QRIADILAQLLQSEDSTEINVVTNSLVTILKSDPK 502 +R+ + A LL E+ + ++TNSL LKS K Sbjct: 110 KRLGYLAAMLLLDENQEVLTLLTNSLQNDLKSRDK 144 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,476,805 Number of Sequences: 5004 Number of extensions: 48708 Number of successful extensions: 135 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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