Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_J01
(654 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g34040.2 68415.m04168 apoptosis inhibitory 5 (API5) family pr... 114 4e-26
At2g34040.1 68415.m04167 apoptosis inhibitory 5 (API5) family pr... 114 4e-26
At1g29030.1 68414.m03553 apoptosis inhibitory 5 (API5) family pr... 95 3e-20
At3g19516.1 68416.m02474 hypothetical protein 60 1e-09
At1g23935.1 68414.m03020 apoptosis inhibitory protein 5 (API5)-r... 35 0.041
At5g15540.1 68418.m01819 expressed protein low similarity to DNA... 32 0.38
At4g31490.1 68417.m04473 coatomer beta subunit, putative / beta-... 32 0.38
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 32 0.38
At1g19870.1 68414.m02492 calmodulin-binding family protein conta... 30 1.2
At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /... 29 2.0
At1g55030.1 68414.m06285 F-box family protein contains F-box dom... 29 2.7
At5g14720.1 68418.m01727 protein kinase family protein contains ... 28 4.7
At4g02920.2 68417.m00396 expressed protein 28 4.7
At4g02920.1 68417.m00395 expressed protein 28 4.7
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 28 4.7
At1g27570.1 68414.m03360 phosphatidylinositol 3- and 4-kinase fa... 28 6.2
At1g15700.1 68414.m01884 ATP synthase gamma chain 2, chloroplast... 28 6.2
At2g25440.1 68415.m03047 leucine-rich repeat family protein cont... 27 8.2
>At2g34040.2 68415.m04168 apoptosis inhibitory 5 (API5) family
protein contains Pfam domain PF05918 Apoptosis
inhibitory protein 5 (API5)
Length = 442
Score = 114 bits (275), Expect = 4e-26
Identities = 62/179 (34%), Positives = 106/179 (59%), Gaps = 1/179 (0%)
Frame = +2
Query: 116 IXKLYKNYDILAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQA 295
I KLY+ + L A+KD+ SQ+ ++Y I+ K S K K+LASQ I ++F FP+LS +A
Sbjct: 12 IEKLYEFSERLNASKDK-SQNVEDYEGIIKMSKTSMKAKQLASQLIPRYFKFFPSLSTEA 70
Query: 296 IEAQFDLCEDDDVAIRKQAIKDLPVLCKEHKXHTQRIADILAQLLQSEDSTEINVVTNSL 475
+A D +D D+ +R QAI+ LP+ CK+ +I D+L QLL +E+ E + V +L
Sbjct: 71 FDAHMDCIDDGDLGVRVQAIRGLPLFCKDTPDILSKIVDVLVQLLNTEEPVERDAVHKAL 130
Query: 476 VTILKSDPKGALSGIFSQIHHNTXGELPNEIVTQRCIKFLSSKIXQLRTE-ISPNEAXE 649
+++L+ DPK + + +F+ H ++ + ++ + F+ K+ L+ E + P E E
Sbjct: 131 MSLLRQDPKASSTALFT---HAGVTPTTDDQIREKVLNFIRDKVIPLKGELLKPQEEME 186
>At2g34040.1 68415.m04167 apoptosis inhibitory 5 (API5) family
protein contains Pfam domain PF05918 Apoptosis
inhibitory protein 5 (API5)
Length = 553
Score = 114 bits (275), Expect = 4e-26
Identities = 62/179 (34%), Positives = 106/179 (59%), Gaps = 1/179 (0%)
Frame = +2
Query: 116 IXKLYKNYDILAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQA 295
I KLY+ + L A+KD+ SQ+ ++Y I+ K S K K+LASQ I ++F FP+LS +A
Sbjct: 12 IEKLYEFSERLNASKDK-SQNVEDYEGIIKMSKTSMKAKQLASQLIPRYFKFFPSLSTEA 70
Query: 296 IEAQFDLCEDDDVAIRKQAIKDLPVLCKEHKXHTQRIADILAQLLQSEDSTEINVVTNSL 475
+A D +D D+ +R QAI+ LP+ CK+ +I D+L QLL +E+ E + V +L
Sbjct: 71 FDAHMDCIDDGDLGVRVQAIRGLPLFCKDTPDILSKIVDVLVQLLNTEEPVERDAVHKAL 130
Query: 476 VTILKSDPKGALSGIFSQIHHNTXGELPNEIVTQRCIKFLSSKIXQLRTE-ISPNEAXE 649
+++L+ DPK + + +F+ H ++ + ++ + F+ K+ L+ E + P E E
Sbjct: 131 MSLLRQDPKASSTALFT---HAGVTPTTDDQIREKVLNFIRDKVIPLKGELLKPQEEME 186
>At1g29030.1 68414.m03553 apoptosis inhibitory 5 (API5) family
protein contains Pfam profile PF05918: Apoptosis
inhibitory protein 5 (API5)
Length = 556
Score = 95.5 bits (227), Expect = 3e-20
Identities = 52/180 (28%), Positives = 102/180 (56%), Gaps = 1/180 (0%)
Frame = +2
Query: 113 NIXKLYKNYDILAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQ 292
+I KLY+ + L+ + D+ SQ+ ++Y I+ K + K K+L +Q I ++F F +L+ +
Sbjct: 11 HIEKLYEFDERLSESTDK-SQNVQDYEGIIELSKTNIKTKQLGAQLIPRYFKFFTSLATE 69
Query: 293 AIEAQFDLCEDDDVAIRKQAIKDLPVLCKEHKXHTQRIADILAQLLQSEDSTEINVVTNS 472
A +A D+ ++ +V +R QAI+ LP+ CK+ +I D+L Q L +E+ E + V +
Sbjct: 70 AFDAYLDIFDEVEVGVRVQAIRGLPLFCKDTPDFISKIIDVLVQCLNTEEFVERDAVHKA 129
Query: 473 LVTILKSDPKGALSGIFSQIHHNTXGELPNEIVTQRCIKFLSSKIXQLRTE-ISPNEAXE 649
L+++ + D K +L+ +F H +E + ++ + F+ K+ L+ E + P + E
Sbjct: 130 LMSLFRQDTKASLTALFK---HTEATLSTDEQIREKVLHFIRDKVFPLKGELLKPQQEME 186
>At3g19516.1 68416.m02474 hypothetical protein
Length = 143
Score = 60.5 bits (140), Expect = 1e-09
Identities = 35/102 (34%), Positives = 55/102 (53%), Gaps = 2/102 (1%)
Frame = +2
Query: 131 KNYDILAAAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQF 310
K+++ E SQ+ K+YL I+ K S + K L + I ++F + + S QA +A
Sbjct: 5 KDFEKRLRESTEKSQNLKDYLGIIEFSKTSIETKELGADLIPRYFQFYTSHSNQAFDAYK 64
Query: 311 DLCEDDDV--AIRKQAIKDLPVLCKEHKXHTQRIADILAQLL 430
D+ E DV +R QAI+ LP+ CK+ +I D+L Q L
Sbjct: 65 DIIEAVDVNLTVRVQAIRKLPLFCKDAPELVSKIIDVLVQCL 106
>At1g23935.1 68414.m03020 apoptosis inhibitory protein 5
(API5)-related contains weak hit to Pfam profile
PF05918: Apoptosis inhibitory protein 5 (API5)
Length = 660
Score = 35.1 bits (77), Expect = 0.041
Identities = 17/85 (20%), Positives = 41/85 (48%)
Frame = +2
Query: 350 AIKDLPVLCKEHKXHTQRIADILAQLLQSEDSTEINVVTNSLVTILKSDPKGALSGIFSQ 529
+I D+ ++CK +I D+L + L +E E + V + ++ D + +L+ + +
Sbjct: 26 SICDIELICKHQPELISKIIDVLVEYLNTEKPVECDAVQEVFMAMVHVDKEASLTAMLNH 85
Query: 530 IHHNTXGELPNEIVTQRCIKFLSSK 604
+ + + +E QR ++ +K
Sbjct: 86 LANFKSLQKESEADLQRLAQYFETK 110
>At5g15540.1 68418.m01819 expressed protein low similarity to DNA
repair and meiosis protein Rad9 [Coprinus cinereus]
GI:1353390, SP|Q09725 Sister chromatid cohesion protein
mis4 {Schizosaccharomyces pombe}
Length = 1755
Score = 31.9 bits (69), Expect = 0.38
Identities = 21/95 (22%), Positives = 44/95 (46%), Gaps = 3/95 (3%)
Frame = +2
Query: 230 KRLASQFIAKFFNSFPTLSEQAIEAQFDLCEDDDVAIRKQAIKDLPVLCKEH---KXHTQ 400
+ A + + + S P + + E + +D V++RK+AIK + +C + T
Sbjct: 832 REAALELVGRHIASHPDVGIKYFEKVAERIKDTGVSVRKRAIKIIRDMCTSNPNFSEFTS 891
Query: 401 RIADILAQLLQSEDSTEINVVTNSLVTILKSDPKG 505
A+IL+++ E S + ++V + +P G
Sbjct: 892 ACAEILSRISDDESSVQ-DLVCKTFYEFWFEEPPG 925
>At4g31490.1 68417.m04473 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 948
Score = 31.9 bits (69), Expect = 0.38
Identities = 31/121 (25%), Positives = 51/121 (42%), Gaps = 2/121 (1%)
Frame = +2
Query: 239 ASQFIAKFFNSFPTLSEQAIEAQFDLCEDDDVAIRKQAIKDLPVLCKEHKXHTQRIADIL 418
+S + + P ++ + EA E +DV + A+K +L + Q I+
Sbjct: 4 SSTMLVHYDKGTPAVANEIKEA----LEGNDVEAKVDAMKKAIMLLLNGETIPQLFITII 59
Query: 419 AQLLQSEDSTEINVVTNSLVTILKSDPKGALSGIFSQIHHNTXGEL--PNEIVTQRCIKF 592
+L SED T ++ L I K+D KG + I N L PNE + ++F
Sbjct: 60 RYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMILICQNLRNNLQHPNEYIRGVTLRF 119
Query: 593 L 595
L
Sbjct: 120 L 120
>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
beta-coat protein, putative / beta-COP, putative similar
to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
from {Rattus norvegicus} SP|P23514, {Mus musculus}
SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
profile: PF01602 Adaptin N terminal region
Length = 971
Score = 31.9 bits (69), Expect = 0.38
Identities = 31/121 (25%), Positives = 51/121 (42%), Gaps = 2/121 (1%)
Frame = +2
Query: 239 ASQFIAKFFNSFPTLSEQAIEAQFDLCEDDDVAIRKQAIKDLPVLCKEHKXHTQRIADIL 418
+S + + P ++ + EA E +DV + A+K +L + Q I+
Sbjct: 27 SSTMLVHYDKGTPAVANEIKEA----LEGNDVEAKVDAMKKAIMLLLNGETIPQLFITII 82
Query: 419 AQLLQSEDSTEINVVTNSLVTILKSDPKGALSGIFSQIHHNTXGEL--PNEIVTQRCIKF 592
+L SED T ++ L I K+D KG + I N L PNE + ++F
Sbjct: 83 RYVLPSEDHTIQKLLLLYLELIEKTDSKGKVLPEMILICQNLRNNLQHPNEYIRGVTLRF 142
Query: 593 L 595
L
Sbjct: 143 L 143
>At1g19870.1 68414.m02492 calmodulin-binding family protein contains
Pfam profile: PF00612 IQ calmodulin-binding motif
Length = 794
Score = 30.3 bits (65), Expect = 1.2
Identities = 39/122 (31%), Positives = 53/122 (43%), Gaps = 3/122 (2%)
Frame = +2
Query: 152 AAKDEISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQFDLCEDDD 331
AAK + E EY++ +K S K K FIA S L DD
Sbjct: 433 AAKPDSLLEEPEYVD--GQIKHSLKRKVSNPSFIAAQSKFEELTSSTGSNKAMTLSSKDD 490
Query: 332 VAIRKQAIK-DLPVLCKEHKXHTQRIADIL-AQLLQSEDSTEINVVTNSLVTI-LKSDPK 502
V + D P K H+ + D+ A+L SE TE++ VT+SL T+ KSD +
Sbjct: 491 VLGEEGKTDIDSPDTTNTIKDHS--LEDVTPAELSGSECGTELS-VTSSLDTLEKKSDAE 547
Query: 503 GA 508
GA
Sbjct: 548 GA 549
>At1g01040.1 68414.m00004 DEAD/DEAH box helicase carpel factory /
CAF identical to RNA helicase/RNAseIII CAF protein
GB:AAF03534 GI:6102610 from [Arabidopsis thaliana]
Length = 1909
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -1
Query: 381 SLHKTGKSFMACLRIATSSSSHKSNWASIACSDKVGK 271
SLH+T K +A + A +SS KS W + D K
Sbjct: 473 SLHETIKQMIAAVEEAAQASSRKSKWQFMGARDAGAK 509
>At1g55030.1 68414.m06285 F-box family protein contains F-box domain
Pfam:PF00646
Length = 422
Score = 29.1 bits (62), Expect = 2.7
Identities = 19/44 (43%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
Frame = -3
Query: 295 CLFG*SR-KTVKKLRNELRCQ--TFLFVRSLYGCEYFQVLFLVL 173
CL G + + V+KL E+ TF F RSLY CE + L L+L
Sbjct: 97 CLIGIAMTRNVRKLVLEVYFHRGTFTFPRSLYHCETLETLELIL 140
>At5g14720.1 68418.m01727 protein kinase family protein contains
eukaryotic protein kinase domain, INTERPRO:IPR000719
Length = 674
Score = 28.3 bits (60), Expect = 4.7
Identities = 28/85 (32%), Positives = 40/85 (47%)
Frame = +2
Query: 263 FNSFPTLSEQAIEAQFDLCEDDDVAIRKQAIKDLPVLCKEHKXHTQRIADILAQLLQSED 442
F SFP QA++ FD+ ED+D A KD V + + T+ LA+ + ED
Sbjct: 403 FASFPIKPLQALKGCFDISEDEDNATTPD-WKDANVNSGQ-QLLTKASIGSLAETTKEED 460
Query: 443 STEINVVTNSLVTILKSDPKGALSG 517
+ N SL + S+ K LSG
Sbjct: 461 TAAQN---TSLPRHVISEQKKYLSG 482
>At4g02920.2 68417.m00396 expressed protein
Length = 419
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +3
Query: 153 PRKMKFHSTRKSTWKYSQP*RDLTKRNVWHLNSLRSFLTVFL 278
P K + S RK K RDL KRN HL++ S L++ +
Sbjct: 251 PEKSRLKSPRKHNTKRKAKERDLYKRN--HLHAYESLLSLMI 290
>At4g02920.1 68417.m00395 expressed protein
Length = 418
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = +3
Query: 153 PRKMKFHSTRKSTWKYSQP*RDLTKRNVWHLNSLRSFLTVFL 278
P K + S RK K RDL KRN HL++ S L++ +
Sbjct: 250 PEKSRLKSPRKHNTKRKAKERDLYKRN--HLHAYESLLSLMI 289
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 28.3 bits (60), Expect = 4.7
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = +2
Query: 164 EISQHEKEYLEILAAVKGSDKEKRLASQFIAKFFNSFPTLSEQAIEAQFDLCE 322
E + E+E L + VKG D++K L I + + +E Q DLC+
Sbjct: 531 EEEEKEEEELTRIKDVKGGDEDKALQEMTIPTASEAQEQARARVLEQQDDLCK 583
>At1g27570.1 68414.m03360 phosphatidylinositol 3- and 4-kinase
family protein contains Pfam profile PF00454:
Phosphatidylinositol 3- and 4-kinase
Length = 649
Score = 27.9 bits (59), Expect = 6.2
Identities = 28/119 (23%), Positives = 50/119 (42%), Gaps = 3/119 (2%)
Frame = +2
Query: 191 LEILAAVKG-SDKEKRLASQFIAK--FFNSFPTLSEQAIEAQFDLCEDDDVAIRKQAIKD 361
L +LAA K D +L+S K + P+LS + +LC +V +R+ I+
Sbjct: 14 LALLAAAKNHGDLAVKLSSLKEVKEILLSLEPSLSAEIFPYLRELCLSPEVLVRRSLIEI 73
Query: 362 LPVLCKEHKXHTQRIADILAQLLQSEDSTEINVVTNSLVTILKSDPKGALSGIFSQIHH 538
+ + H+ + +L L+ D T ++ T +S L + +Q HH
Sbjct: 74 IEEVGLRMLEHSYVLVSVLIHLVGDNDPTVAEKSISTGTTFFRS----ILEKMETQFHH 128
>At1g15700.1 68414.m01884 ATP synthase gamma chain 2, chloroplast
(ATPC2) identical to SP|Q01909 ATP synthase gamma chain
2, chloroplast precursor (EC 3.6.3.14) {Arabidopsis
thaliana}; contains Pfam profile: PF00231 ATP synthase;
similar to ATP synthase gamma-subunit GI:21241 from
[Spinacia oleracea]
Length = 386
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 398 QRIADILAQLLQSEDSTEINVVTNSLVTILKSDP 499
Q IAD + L SE+ ++ +V V+++KSDP
Sbjct: 212 QVIADDVFSLFVSEEVDKVELVYTKFVSLVKSDP 245
>At2g25440.1 68415.m03047 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to NL0E
[Lycopersicon esculentum] gi|4235643|gb|AAD13303
Length = 671
Score = 27.5 bits (58), Expect = 8.2
Identities = 22/72 (30%), Positives = 36/72 (50%), Gaps = 7/72 (9%)
Frame = +2
Query: 437 EDSTEINVVTNSLVTILKSDP-KGALSGI------FSQIHHNTXGELPNEIVTQRCIKFL 595
E STE+ V ++ + +L+S+ +GAL + FS H+N GE+P I T+ + L
Sbjct: 242 EGSTEVLVNSSVRILLLESNNFEGALPSLPHSINAFSAGHNNFTGEIPLSICTRTSLGVL 301
Query: 596 SSKIXQLRTEIS 631
L +S
Sbjct: 302 DLNYNNLIGPVS 313
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,398,483
Number of Sequences: 28952
Number of extensions: 235849
Number of successful extensions: 733
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 710
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 733
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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