Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_I15
(655 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual 49 5e-07
SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr 1|||... 37 0.003
SPBC336.05c |||S-adenosylmethionine-dependentmethyltransferase|S... 32 0.063
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 27 3.1
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 26 5.5
SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2 |... 26 5.5
SPBC947.13 |rba50||RNA polymerase II associated protein |Schizos... 25 9.5
SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces pom... 25 9.5
SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces ... 25 9.5
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 25 9.5
>SPCC965.09 |||nitrilase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 272
Score = 49.2 bits (112), Expect = 5e-07
Identities = 28/87 (32%), Positives = 48/87 (55%)
Frame = +2
Query: 386 NIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILXXDEK 565
N+I F EL + C + + AE A +GP+ + LA KY + I+ +EK
Sbjct: 39 NLILFPELITSGYE-CGNT---FTQIAEIAGEGPSFKTMSNLAAKYHVNIIYGFPEKEEK 94
Query: 566 HSDILWNTAVVISDTGNVIGKHRKNHI 646
S+I++N+ + I++ GN+ G +RK H+
Sbjct: 95 QSNIIYNSCIYITENGNLGGVYRKVHL 121
>SPAC26A3.11 |||amidohydrolase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 322
Score = 36.7 bits (81), Expect = 0.003
Identities = 34/127 (26%), Positives = 59/127 (46%)
Frame = +2
Query: 266 KVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREK 445
++G+VQ +A D+ N Q + K+++ A + G N+I E++N P+ T
Sbjct: 45 RIGLVQ--LANTKDKSENLQLARL-----KVLEAA-KNGSNVIVLPEIFNSPYG--TGYF 94
Query: 446 QPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILXXDEKHSDILWNTAVVISDTGNVIG 625
+ E E E P+ L +A + + E+ L+NTA+V +G +I
Sbjct: 95 NQYAEPIE--ESSPSYQALSSMAKDTKTYLFGGSIP--ERKDGKLYNTAMVFDPSGKLIA 150
Query: 626 KHRKNHI 646
HRK H+
Sbjct: 151 VHRKIHL 157
>SPBC336.05c |||S-adenosylmethionine-
dependentmethyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 378
Score = 32.3 bits (70), Expect = 0.063
Identities = 21/64 (32%), Positives = 31/64 (48%)
Frame = -2
Query: 498 KVVVGPSSADSANSHHGCFSLVQNAKGMFHNSWKQMMLTPSWPATSMIFLTLLKIAFFCS 319
++ V S ++A SH CF QN+ + + + +P T F+ LLK AFF
Sbjct: 232 QIAVFHQSKNNAASH--CFLKDQNSSILLYKKITYPFMEQLFPPTVQQFMNLLKKAFFDH 289
Query: 318 LTGR 307
L GR
Sbjct: 290 LFGR 293
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 470 SAEDGPTTTFLRELAIKYAMVIVSSILXXDEKHSDIL 580
SAE+ + + + +++VS++L DEKH D++
Sbjct: 979 SAENTTSFSIFAAQGLTDFLIVVSNLLEMDEKHVDVV 1015
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.8 bits (54), Expect = 5.5
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2
Query: 89 NNLTGRDLEEFNRIHFGRRNNLEI 160
NN+ R +EE N I G+R LE+
Sbjct: 8 NNILKRHIEEDNNIDNGKRKKLEL 31
>SPAC3G9.06 |frs2||phenylalanine-tRNA ligase alpha subunit Frs2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 499
Score = 25.8 bits (54), Expect = 5.5
Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = +2
Query: 59 ETHSLESII-NNNLTGRDLEEFNRIHFGRRNNLEIKLK 169
E H +E +I + N+T DL F + FG+ N ++ K
Sbjct: 369 EFHQVEGVICDRNITLGDLIGFLEVFFGKMNVKNLRFK 406
>SPBC947.13 |rba50||RNA polymerase II associated protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 452
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2
Query: 92 NLTGRDLEEFNRIHFGRRNNLEIKLKESSI 181
NL + ++E NR+ R N+LEI+ + I
Sbjct: 74 NLDDQGIDEENRVRLERMNDLEIEGAQEEI 103
>SPAC24B11.07c |||ketopantoate reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 561
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = -1
Query: 418 HVPQLLETDDVNTLLAGNIDDFLDFIENC 332
++P+LLE D+N L G + + LD + NC
Sbjct: 222 NLPKLLERPDINDLHQGILQE-LDSLCNC 249
>SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 580
Score = 25.0 bits (52), Expect = 9.5
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = -2
Query: 654 TLGMWFLRCFPITFPVSLITTAVFQSMSECFSSXXNIEDTITIAYLMA 511
TLG +L F T+P+ LIT ++ C NI I I +A
Sbjct: 163 TLGWNYLASFLATYPLELITASI------CLQFWININSGIWITVFIA 204
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.0 bits (52), Expect = 9.5
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = -1
Query: 349 DFIENCFLLLVDWTIGGHRDGMLNYSYLHNXRGSGLLVLGRESVCGDVEVSL 194
+FI+ CF + + GH D +++ S + GS +G S D++VSL
Sbjct: 661 EFIQRCFHFADEASPDGHSDTLIDISDHMSSTGSENRSVGANS---DIKVSL 709
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,582,223
Number of Sequences: 5004
Number of extensions: 49966
Number of successful extensions: 173
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 167
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 172
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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