Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_I15
(655 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 203 9e-53
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 54 8e-08
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 54 8e-08
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 48 7e-06
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 46 2e-05
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 31 0.51
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 31 0.67
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 31 0.88
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 2.7
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 29 3.6
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 28 6.2
At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to ... 27 8.2
At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to ... 27 8.2
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 203 bits (495), Expect = 9e-53
Identities = 103/199 (51%), Positives = 132/199 (66%), Gaps = 3/199 (1%)
Frame = +2
Query: 68 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 238
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 239 EQTRPPXIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 418
EQ R P +V+VG++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 419 PFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILXXDEKHSDILWNTAVV 598
PFAFCTRE++ WCEFAE DG +T FL+ELA KY MVIVS IL D H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 599 ISDTGNVIGKHRKNHIPRV 655
I + GN+IGKHRKNHIPRV
Sbjct: 200 IGNNGNIIGKHRKNHIPRV 218
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 54.0 bits (124), Expect = 8e-08
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +2
Query: 314 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 487
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 488 TTTFLRELAIKYAMVIVSSILXXDEKHSDILWNTAVVISDTGNVIGKHRKNHI 646
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 54.0 bits (124), Expect = 8e-08
Identities = 32/113 (28%), Positives = 55/113 (48%), Gaps = 2/113 (1%)
Frame = +2
Query: 314 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 487
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 488 TTTFLRELAIKYAMVIVSSILXXDEKHSDILWNTAVVISDTGNVIGKHRKNHI 646
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHL 204
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 47.6 bits (108), Expect = 7e-06
Identities = 28/100 (28%), Positives = 56/100 (56%)
Frame = +2
Query: 350 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAM 529
++++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 530 VIVSSILXXDEKHSDILWNTAVVISDTGNVIGKHRKNHIP 649
VI S E+ + +N+ +I G +G +RK+HIP
Sbjct: 90 VIPVSFF---EEANTAHYNSIAIIDADGTDLGIYRKSHIP 126
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 46.4 bits (105), Expect = 2e-05
Identities = 28/98 (28%), Positives = 54/98 (55%)
Frame = +2
Query: 356 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVI 535
++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 536 VSSILXXDEKHSDILWNTAVVISDTGNVIGKHRKNHIP 649
S E+ + +N+ +I G +G +RK+HIP
Sbjct: 119 PVSFF---EEANTAHYNSIAIIDADGTDLGIYRKSHIP 153
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 31.5 bits (68), Expect = 0.51
Identities = 43/142 (30%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Frame = +2
Query: 251 PPXIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW------ 412
P V+V IVQ S V D P A +K +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 413 --NMPFAFCTREKQPWCEFAE---SA--EDGPTTTFLRELAIKYAMVIVSSILXXDEKHS 571
A ++ EF SA GP L ELA K + +V + EK
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAI---EKDG 130
Query: 572 DILWNTAVVISDTGNVIGKHRK 637
L+ TA+ S G +GKHRK
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK 152
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 31.1 bits (67), Expect = 0.67
Identities = 20/52 (38%), Positives = 26/52 (50%)
Frame = +2
Query: 482 GPTTTFLRELAIKYAMVIVSSILXXDEKHSDILWNTAVVISDTGNVIGKHRK 637
GP L ELA K + +V + EK L+ TA+ S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAI---EKDGYTLYCTALFFSPQGQFLGKHRK 145
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 30.7 bits (66), Expect = 0.88
Identities = 29/104 (27%), Positives = 45/104 (43%), Gaps = 1/104 (0%)
Frame = +2
Query: 338 FNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAI 517
F +++ A G +ICF E F+F ++ + AE DGP R ++
Sbjct: 54 FATCSRLVQEAALAGAKLICFPE----NFSFVGDKEGESVKIAEPL-DGPVME--RYCSL 106
Query: 518 KYAMVIVSSILXXDEKHSDI-LWNTAVVISDTGNVIGKHRKNHI 646
I S+ E+ D L NT VVI D G + ++K H+
Sbjct: 107 ARDSNIWLSLGGFQERFDDTHLCNTHVVIDDAGMIRDTYQKMHL 150
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.1 bits (62), Expect = 2.7
Identities = 39/142 (27%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Frame = +2
Query: 251 PPXIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE--LWNMPF 424
P V+V IVQ S V D P A +K +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 425 AFC---------TREKQPWCEFAESA--EDGPTTTFLRELAIKYAMVIVSSILXXDEKHS 571
F + + ++ SA GP L ++A K + +V + EK
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAI---EKEG 130
Query: 572 DILWNTAVVISDTGNVIGKHRK 637
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK 152
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 28.7 bits (61), Expect = 3.6
Identities = 28/115 (24%), Positives = 50/115 (43%), Gaps = 13/115 (11%)
Frame = +2
Query: 332 AIFNKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 478
A +K ++++ A + G ++ F E + A +R + +F + SA
Sbjct: 52 ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111
Query: 479 D--GPTTTFLRELAIKYAMVIVSSILXXDEKHSDILWNTAVVISDTGNVIGKHRK 637
D GP L +A KY + +V ++ E+ L+ T + G +GKHRK
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVI---EREGYTLYCTVLFFDSQGLFLGKHRK 163
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 27.9 bits (59), Expect = 6.2
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +1
Query: 490 HDLPSGTRHQVRNGDRVLDIXXGREAFGH 576
HD+PS H V NGD LD+ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At1g28610.2 68414.m03523 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 383
Score = 27.5 bits (58), Expect = 8.2
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 599 ISDTGNVIGKHRKNHIP 649
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
>At1g28610.1 68414.m03524 GDSL-motif lipase, putative similar to
lipase [Arabidopsis thaliana] GI:1145627; contains Pfam
profile: PF00657 Lipase/Acylhydrolase with GDSL-like
motif
Length = 250
Score = 27.5 bits (58), Expect = 8.2
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2
Query: 599 ISDTGNVIGKHRKNHIP 649
I+DTGN++G +NH+P
Sbjct: 39 ITDTGNLVGLSDRNHLP 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,848,509
Number of Sequences: 28952
Number of extensions: 275764
Number of successful extensions: 795
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 771
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 791
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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