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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P02_F_H16
         (656 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...   101   9e-23
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    68   1e-12
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    66   4e-12
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    60   2e-10
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    60   3e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    59   6e-10
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    57   3e-09
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    56   3e-09
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    56   4e-09
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    50   2e-07
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    44   1e-05
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    40   2e-04
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    34   0.021
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...    32   0.063
SPBC146.09c |lsd1|swm1, saf110|histone demethylase SWIRM1|Schizo...    30   0.34 
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz...    27   2.4  
SPBC4C3.08 |mug136||acetylglucosaminyltransferase|Schizosaccharo...    25   7.3  

>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score =  101 bits (242), Expect = 9e-23
 Identities = 43/68 (63%), Positives = 51/68 (75%)
 Frame = +2

Query: 452 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFK 631
           KRI +ELA++  DPP +CSAGP GD+L+ W +TI+GP  S Y GGVFFL IHF   YPFK
Sbjct: 4   KRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63

Query: 632 PPKVTFRT 655
           PPKV F T
Sbjct: 64  PPKVNFTT 71


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 67.7 bits (158), Expect = 1e-12
 Identities = 30/77 (38%), Positives = 43/77 (55%)
 Frame = +2

Query: 425 MSKALGTSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDI 604
           M+ A  +S++R+ KE +++   P P+       DNL+ W  T LGP  SVY GG F   +
Sbjct: 1   MASASPSSSRRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSL 60

Query: 605 HFSPXYPFKPPKVTFRT 655
            F   YPF+PP + F T
Sbjct: 61  KFPLDYPFQPPTIEFTT 77


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 66.1 bits (154), Expect = 4e-12
 Identities = 32/68 (47%), Positives = 38/68 (55%)
 Frame = +2

Query: 452 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFK 631
           KRI KE+  +T DPPP   A P  DNL  +  T+ GP  S YEGG F L++     YP  
Sbjct: 5   KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMM 64

Query: 632 PPKVTFRT 655
           PP V F T
Sbjct: 65  PPNVRFLT 72


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 28/71 (39%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
 Frame = +2

Query: 446 SAKRIQKELAEITLDPPPNCSAGPKGD-NLYEWVSTILGPPGSVYEGGVFFLDIHFSPXY 622
           S + ++K+L EI  +PP   S G   D +++EW   I+GP  ++YEGG F   + F   Y
Sbjct: 6   SEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDY 65

Query: 623 PFKPPKVTFRT 655
           P  PPK+ F T
Sbjct: 66  PLMPPKMKFTT 76


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 26/69 (37%), Positives = 38/69 (55%)
 Frame = +2

Query: 443 TSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXY 622
           T+ +R+ ++   +  DPP   SA P  DN+  W + I+GP  + +E G F L + F   Y
Sbjct: 4   TARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQY 63

Query: 623 PFKPPKVTF 649
           P KPP V F
Sbjct: 64  PNKPPLVKF 72


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 58.8 bits (136), Expect = 6e-10
 Identities = 27/68 (39%), Positives = 36/68 (52%)
 Frame = +2

Query: 452 KRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFK 631
           KR+ KE   +  +P     A P  +N+ EW   I GPP + YEGG +   + F P YPFK
Sbjct: 8   KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67

Query: 632 PPKVTFRT 655
           PP +   T
Sbjct: 68  PPAIRMIT 75


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 28/76 (36%), Positives = 43/76 (56%), Gaps = 1/76 (1%)
 Frame = +2

Query: 425 MSKALGTSAKRIQKELAEITLDPPPNCSAGPKG-DNLYEWVSTILGPPGSVYEGGVFFLD 601
           MSKA+  + +R+ KE  E+T + P   +AGP   D+ + W   I GP G+ +EGG++   
Sbjct: 1   MSKAM--ALRRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPAT 58

Query: 602 IHFSPXYPFKPPKVTF 649
           + F   YP  PP + F
Sbjct: 59  LKFPSDYPLGPPTLKF 74


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 56.4 bits (130), Expect = 3e-09
 Identities = 26/67 (38%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
 Frame = +2

Query: 452 KRIQKELAEITLDPPPNCSAGPKGD-NLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPF 628
           KR++ EL  + +   P  SA P  D NL  W  TI GP  + YEG  F + + F   YP+
Sbjct: 31  KRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPY 90

Query: 629 KPPKVTF 649
            PP + F
Sbjct: 91  SPPTIIF 97


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 56.0 bits (129), Expect = 4e-09
 Identities = 26/69 (37%), Positives = 36/69 (52%)
 Frame = +2

Query: 449 AKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPF 628
           ++RI KELA++  D           D++        GP G+ YEGG F +DI     YPF
Sbjct: 6   SRRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPF 65

Query: 629 KPPKVTFRT 655
           +PPK+ F T
Sbjct: 66  RPPKMNFDT 74


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 28/75 (37%), Positives = 38/75 (50%), Gaps = 2/75 (2%)
 Frame = +2

Query: 437 LGTSAKRIQKELAEITL-DPPPNCSAGPKGD-NLYEWVSTILGPPGSVYEGGVFFLDIHF 610
           + +S KR+ KE A     D        P  D +++ W + I GP  + YEGG + LDIH 
Sbjct: 1   MNSSIKRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHV 60

Query: 611 SPXYPFKPPKVTFRT 655
              YP  PP V F+T
Sbjct: 61  HEGYPISPPSVYFQT 75


>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 44.4 bits (100), Expect = 1e-05
 Identities = 20/40 (50%), Positives = 25/40 (62%)
 Frame = +2

Query: 530 LYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFKPPKVTF 649
           L +W +TILGP  SV+E  ++ L IH    YP  PP VTF
Sbjct: 38  LSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTF 77


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 40.3 bits (90), Expect = 2e-04
 Identities = 18/49 (36%), Positives = 26/49 (53%)
 Frame = +2

Query: 503 CSAGPKGDNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFKPPKVTF 649
           C +   G +L  W   I G P + +EGG++ L + F   YP +PPK  F
Sbjct: 29  CKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRF 77


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 33.9 bits (74), Expect = 0.021
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +2

Query: 524 DNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFKPPKVTF 649
           DN+ E+     GP  + Y GG++ + +     YP+K P + F
Sbjct: 27  DNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGF 68


>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score = 32.3 bits (70), Expect = 0.063
 Identities = 21/81 (25%), Positives = 35/81 (43%)
 Frame = +2

Query: 401 KENKPNHKMSKALGTSAKRIQKELAEITLDPPPNCSAGPKGDNLYEWVSTILGPPGSVYE 580
           K+     K +  +  +  RIQK++ ++ + P    ++ P    L   +   + P    Y+
Sbjct: 9   KKEAQKEKNASGISPAQIRIQKDVTDLEI-PSTMSTSWPDPIKL-NVLHLEIRPDEGYYK 66

Query: 581 GGVFFLDIHFSPXYPFKPPKV 643
           GG F   I     YP  PPKV
Sbjct: 67  GGKFKFRIQIDDNYPHDPPKV 87


>SPBC146.09c |lsd1|swm1, saf110|histone demethylase
           SWIRM1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1000

 Score = 29.9 bits (64), Expect = 0.34
 Identities = 12/31 (38%), Positives = 18/31 (58%)
 Frame = -2

Query: 244 HLIVSRHPHQHKQLRNFSRCHFVNSESL*II 152
           HLI    P + K+L+   RCHF N + + +I
Sbjct: 578 HLITFEPPLEEKKLKAIDRCHFTNVKKVILI 608


>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 255

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = -3

Query: 624 GYXGEK*MSRKNTPPSYTEPGGPRMVDT-HSYKLS 523
           G+  EK +  K TP    +PG P +  T H YK++
Sbjct: 216 GFQIEKILEEKRTPMFEEDPGAPEIRATVHGYKMT 250


>SPBC4C3.08 |mug136||acetylglucosaminyltransferase|Schizosaccharomyc
           es pombe|chr 2|||Manual
          Length = 372

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 13/43 (30%), Positives = 21/43 (48%)
 Frame = +2

Query: 524 DNLYEWVSTILGPPGSVYEGGVFFLDIHFSPXYPFKPPKVTFR 652
           D+LY +V   +  PG  +E    F D   +  + FKP K  ++
Sbjct: 231 DDLYPYVFAAVSDPGMWHETPPPFKDYFNAGLFVFKPLKAHYK 273


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,643,426
Number of Sequences: 5004
Number of extensions: 54368
Number of successful extensions: 143
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 141
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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