Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_G24
(652 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 233 2e-62
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 134 1e-32
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 129 4e-31
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 124 1e-29
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 28 1.0
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.3
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.3
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 3.1
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 4.1
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 5.4
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.2
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 233 bits (570), Expect = 2e-62
Identities = 100/132 (75%), Positives = 116/132 (87%)
Frame = +1
Query: 91 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 270
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 271 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAXGHYTEGAELVDSVLDVV 450
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WA GHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 451 RKEAESCDCLQG 486
R+EAE+CD LQG
Sbjct: 121 RREAEACDALQG 132
Score = 56.4 bits (130), Expect = 3e-09
Identities = 25/34 (73%), Positives = 28/34 (82%)
Frame = +2
Query: 551 KIXXXYPDRIMNTYSVVPSPKVSDTVVEPYNATL 652
KI YPDR+M T+SV P+PK SDTVVEPYNATL
Sbjct: 154 KIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 134 bits (323), Expect = 1e-32
Identities = 62/134 (46%), Positives = 84/134 (62%), Gaps = 2/134 (1%)
Frame = +1
Query: 91 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 264
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 265 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAXGHYTEGAELVDSVLD 444
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 445 VVRKEAESCDCLQG 486
+R+ A++C LQG
Sbjct: 121 KIRRIADNCSGLQG 134
Score = 33.5 bits (73), Expect = 0.027
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = +2
Query: 551 KIXXXYPDRIMNTYSVVPSPKVSDTVVEPYNATL 652
++ Y + +SV P+P+VS +VVEPYN+ L
Sbjct: 156 RLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVL 189
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 129 bits (311), Expect = 4e-31
Identities = 59/138 (42%), Positives = 85/138 (61%), Gaps = 6/138 (4%)
Frame = +1
Query: 91 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 252
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 253 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAXGHYTEGAELVD 432
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 433 SVLDVVRKEAESCDCLQG 486
SVL+ +R+ A++C LQG
Sbjct: 121 SVLERIRRMADNCSGLQG 138
Score = 33.1 bits (72), Expect = 0.036
Identities = 13/21 (61%), Positives = 18/21 (85%)
Frame = +2
Query: 590 YSVVPSPKVSDTVVEPYNATL 652
+SV P+P+VS +VVEPYN+ L
Sbjct: 173 FSVYPAPQVSTSVVEPYNSVL 193
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 124 bits (298), Expect = 1e-29
Identities = 52/133 (39%), Positives = 90/133 (67%), Gaps = 2/133 (1%)
Frame = +1
Query: 94 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 273
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 274 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAXGHYTEGAELVDSVLDV 447
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 448 VRKEAESCDCLQG 486
+ +EA+ D L+G
Sbjct: 122 IDREADGSDSLEG 134
Score = 39.1 bits (87), Expect = 5e-04
Identities = 18/35 (51%), Positives = 24/35 (68%), Gaps = 1/35 (2%)
Frame = +2
Query: 551 KIXXXYPDRIMNTYSVVP-SPKVSDTVVEPYNATL 652
++ YP +I+ TYSV P S VSD VV+PYN+ L
Sbjct: 156 RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLL 190
>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 879
Score = 28.3 bits (60), Expect = 1.0
Identities = 14/40 (35%), Positives = 27/40 (67%)
Frame = -2
Query: 138 DLVAALTSLDMYDFPHFVLFM*TINYVNTKNXLRTLHSLS 19
D+++ TSL + + P L+M + + ++TK L+TLH+L+
Sbjct: 123 DILSTGTSLRLANAPTSKLYMLSESPLSTKQILQTLHALN 162
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 130 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 231
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 27.1 bits (57), Expect = 2.3
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +1
Query: 325 GPFGQIFRPDNFVFGQSGAG-NNWAXGHYTEGAEL 426
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.6 bits (56), Expect = 3.1
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +1
Query: 394 AXGHYTEGAELVDSVLDVVRKEAESCDCLQGIP 492
A GH G ELV + D +RK++E+ L+ P
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALEVCP 215
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.1
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 467 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 330
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 5.4
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 346 RPDNFVFGQSGAGNNWAXGHYTEGAELVDSVLDVVRKEAESCDC 477
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.2
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -3
Query: 383 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 288
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,302,668
Number of Sequences: 5004
Number of extensions: 41424
Number of successful extensions: 115
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 293780908
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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