BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_F24 (652 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei... 134 6e-32 At5g61100.1 68418.m07666 hypothetical protein 30 1.2 At4g36980.1 68417.m05240 expressed protein 29 2.0 At1g54130.1 68414.m06171 RelA/SpoT protein, putative (RSH3) iden... 27 8.2 >At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein similar to SP|P12048 Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142: MGS-like domain Length = 596 Score = 134 bits (323), Expect = 6e-32 Identities = 70/145 (48%), Positives = 92/145 (63%), Gaps = 3/145 (2%) Frame = +3 Query: 219 QNMASNG-KLALLSVSDKTGLLSLAKSLSECGLQLIASGGTATALRNAGLTVQDVSDITR 395 Q+ S+G K AL+S+SDK L SL L E G ++++GGTA+ L NAG++V V +T Sbjct: 64 QSSGSSGEKQALISLSDKRDLASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTH 123 Query: 396 APEMLGGRVKTLHPAVHAGILXRLS-DSDQEDMKRQKYEMISVVVCNLYPFVQTVSKP-D 569 PEML GRVKTLHP +H GIL R + E + VVV NLYPF + V+ P Sbjct: 124 FPEMLDGRVKTLHPNIHGGILARRDVEHHMEALNEHGIGTFDVVVVNLYPFYEKVTAPGG 183 Query: 570 VTVADAVENIDIGGVTLLRAAAKNH 644 ++ D +ENIDIGG ++RAAAKNH Sbjct: 184 ISFEDGIENIDIGGPAMIRAAAKNH 208 >At5g61100.1 68418.m07666 hypothetical protein Length = 227 Score = 30.3 bits (65), Expect = 1.2 Identities = 14/45 (31%), Positives = 25/45 (55%) Frame = +1 Query: 376 MCRTSREHRRCSEVG*KLYIQRYMLGSXLDYPTLTRKT*NVRSTR 510 MCR +REH C+ V + + ++ D+ ++ KT N +S+R Sbjct: 51 MCRDTREHTYCARVMFQRVPRLWICEECRDFSSVANKTANAQSSR 95 >At4g36980.1 68417.m05240 expressed protein Length = 560 Score = 29.5 bits (63), Expect = 2.0 Identities = 16/48 (33%), Positives = 19/48 (39%) Frame = -2 Query: 534 GYRPPRLSSRTSDVSCLPGQSRIIEXRSQHVPLDVKFSPDLRASPVLS 391 GY PPR SD+ P RI+E D+ D A P S Sbjct: 355 GYSPPRSPPSQSDLLSRPSPGRILEALHVDPASDISLEKDKVAKPAKS 402 >At1g54130.1 68414.m06171 RelA/SpoT protein, putative (RSH3) identical to RSH3 (RelA/SpoT homolog) GI:7141308 from [Arabidopsis thaliana]; contains Pfam profiles PF01966: HD domain, PF04607: Region found in RelA / SpoT proteins Length = 712 Score = 27.5 bits (58), Expect = 8.2 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 2/35 (5%) Frame = +2 Query: 266 QDGSTLVSKEPVGMWP--AVDCQWRYRHGASERRP 364 +DGS++ S EP+ +P A DC + Y+ ++ P Sbjct: 565 KDGSSICSSEPLCSFPSHAEDCPFSYKPSGNQEGP 599 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,577,477 Number of Sequences: 28952 Number of extensions: 241346 Number of successful extensions: 709 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 699 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 707 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1354097952 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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