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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P02_F_F24
         (652 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei...   134   6e-32
At5g61100.1 68418.m07666 hypothetical protein                          30   1.2  
At4g36980.1 68417.m05240 expressed protein                             29   2.0  
At1g54130.1 68414.m06171 RelA/SpoT protein, putative (RSH3) iden...    27   8.2  

>At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein
           similar to SP|P12048 Bifunctional purine biosynthesis
           protein purH [Includes:
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase (EC 2.1.2.3) (AICAR transformylase);
           IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP
           synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam
           profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142:
           MGS-like domain
          Length = 596

 Score =  134 bits (323), Expect = 6e-32
 Identities = 70/145 (48%), Positives = 92/145 (63%), Gaps = 3/145 (2%)
 Frame = +3

Query: 219 QNMASNG-KLALLSVSDKTGLLSLAKSLSECGLQLIASGGTATALRNAGLTVQDVSDITR 395
           Q+  S+G K AL+S+SDK  L SL   L E G  ++++GGTA+ L NAG++V  V  +T 
Sbjct: 64  QSSGSSGEKQALISLSDKRDLASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTH 123

Query: 396 APEMLGGRVKTLHPAVHAGILXRLS-DSDQEDMKRQKYEMISVVVCNLYPFVQTVSKP-D 569
            PEML GRVKTLHP +H GIL R   +   E +         VVV NLYPF + V+ P  
Sbjct: 124 FPEMLDGRVKTLHPNIHGGILARRDVEHHMEALNEHGIGTFDVVVVNLYPFYEKVTAPGG 183

Query: 570 VTVADAVENIDIGGVTLLRAAAKNH 644
           ++  D +ENIDIGG  ++RAAAKNH
Sbjct: 184 ISFEDGIENIDIGGPAMIRAAAKNH 208


>At5g61100.1 68418.m07666 hypothetical protein
          Length = 227

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 14/45 (31%), Positives = 25/45 (55%)
 Frame = +1

Query: 376 MCRTSREHRRCSEVG*KLYIQRYMLGSXLDYPTLTRKT*NVRSTR 510
           MCR +REH  C+ V  +   + ++     D+ ++  KT N +S+R
Sbjct: 51  MCRDTREHTYCARVMFQRVPRLWICEECRDFSSVANKTANAQSSR 95


>At4g36980.1 68417.m05240 expressed protein
          Length = 560

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 16/48 (33%), Positives = 19/48 (39%)
 Frame = -2

Query: 534 GYRPPRLSSRTSDVSCLPGQSRIIEXRSQHVPLDVKFSPDLRASPVLS 391
           GY PPR     SD+   P   RI+E        D+    D  A P  S
Sbjct: 355 GYSPPRSPPSQSDLLSRPSPGRILEALHVDPASDISLEKDKVAKPAKS 402


>At1g54130.1 68414.m06171 RelA/SpoT protein, putative (RSH3)
           identical to RSH3 (RelA/SpoT homolog) GI:7141308 from
           [Arabidopsis thaliana]; contains Pfam profiles PF01966:
           HD domain, PF04607: Region found in RelA / SpoT proteins
          Length = 712

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 2/35 (5%)
 Frame = +2

Query: 266 QDGSTLVSKEPVGMWP--AVDCQWRYRHGASERRP 364
           +DGS++ S EP+  +P  A DC + Y+   ++  P
Sbjct: 565 KDGSSICSSEPLCSFPSHAEDCPFSYKPSGNQEGP 599


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,577,477
Number of Sequences: 28952
Number of extensions: 241346
Number of successful extensions: 709
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 707
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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