Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_E12
(648 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 42 5e-04
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 41 8e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 39 0.003
At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17... 38 0.006
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 37 0.010
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 35 0.054
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 33 0.16
At3g49540.1 68416.m05414 expressed protein 29 2.7
At5g04290.1 68418.m00422 KOW domain-containing transcription fac... 28 6.1
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 8.1
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 41.5 bits (93), Expect = 5e-04
Identities = 31/93 (33%), Positives = 49/93 (52%), Gaps = 8/93 (8%)
Frame = +2
Query: 320 DVQHFAPEEISVKTADGYIV-VEGK----HXEKKDQHGYISR---QFTRRYALPEGCTAE 475
D+ EE+ V+ DG I+ + G+ + EK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 476 SVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 574
+++ + +GVLSV P KVP + I I+
Sbjct: 126 EIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 40.7 bits (91), Expect = 8e-04
Identities = 30/104 (28%), Positives = 55/104 (52%), Gaps = 8/104 (7%)
Frame = +2
Query: 287 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHXEKK-DQHGYISR---QFTR 442
K + ++ LD+ +E+ ++ + G + V G + EKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 443 RYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKIPIA 574
++ LP+ ESV+++L +GVL++ + P V+G R + IA
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTINLTKLSPEKVKGPRVVNIA 178
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 38.7 bits (86), Expect = 0.003
Identities = 27/78 (34%), Positives = 41/78 (52%), Gaps = 8/78 (10%)
Frame = +2
Query: 320 DVQHFAPEEISVKTADGYIV-VEGK----HXEKKDQHGYISR---QFTRRYALPEGCTAE 475
DV EE+ V+ DG I+ + G+ + EK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 476 SVESRLSSDGVLSVIAPR 529
V++ + +GVLSV P+
Sbjct: 125 EVKASM-ENGVLSVTVPK 141
>At5g12030.1 68418.m01406 17.7 kDa class II heat shock protein 17.6A
(HSP17.7-CII) identical to heat shock protein 17.6A
GI:3256075 from [Arabidopsis thaliana]
Length = 156
Score = 37.9 bits (84), Expect = 0.006
Identities = 34/126 (26%), Positives = 57/126 (45%), Gaps = 9/126 (7%)
Frame = +2
Query: 221 SREYYRPWRHLAAAARDLGSSIKSDKDKFQVNLDVQHFAPEEISVKTA-DGYIVVEGKHX 397
SR Y R + +AA D + D + +D+ +EI V+ + +VV GK
Sbjct: 33 SRAYMRDAKAMAATPAD----VIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVVSGKRQ 88
Query: 398 -EKKDQHGY----ISRQ---FTRRYALPEGCTAESVESRLSSDGVLSVIAPRKVPPAVEG 553
+ K+ G + R+ F R++ LP+ E + S +DGVL V P+ PP +
Sbjct: 89 RDNKENEGVKFVRMERRMGKFMRKFQLPDNADLEKI-SAACNDGVLKVTIPKLPPPEPKK 147
Query: 554 ERKIPI 571
+ I +
Sbjct: 148 PKTIQV 153
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 37.1 bits (82), Expect = 0.010
Identities = 25/78 (32%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +2
Query: 320 DVQHFAPEEISVKTA-DGYIVVEG-KHXEKKDQHGYISR------QFTRRYALPEGCTAE 475
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 476 SVESRLSSDGVLSVIAPR 529
V++ + +GVL+V P+
Sbjct: 122 QVKAAM-ENGVLTVTVPK 138
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 34.7 bits (76), Expect = 0.054
Identities = 23/78 (29%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +2
Query: 320 DVQHFAPEEISVKTADGYIV-VEGK----HXEKKDQHGYISR---QFTRRYALPEGCTAE 475
D+ EE+ V+ D ++ + G+ + EK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 476 SVESRLSSDGVLSVIAPR 529
V++ + +GVL+V+ P+
Sbjct: 128 EVKATM-ENGVLTVVVPK 144
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 33.1 bits (72), Expect = 0.16
Identities = 22/78 (28%), Positives = 42/78 (53%), Gaps = 8/78 (10%)
Frame = +2
Query: 320 DVQHFAPEEISVKTADGYIV-VEG-KHXEKKDQHGYISR------QFTRRYALPEGCTAE 475
D+ EE+ V+ D ++ + G +H EK+++ R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 476 SVESRLSSDGVLSVIAPR 529
V++ + +GVL+V P+
Sbjct: 124 QVKASM-ENGVLTVTVPK 140
>At3g49540.1 68416.m05414 expressed protein
Length = 166
Score = 29.1 bits (62), Expect = 2.7
Identities = 23/70 (32%), Positives = 39/70 (55%), Gaps = 7/70 (10%)
Frame = +2
Query: 458 EGCTAESVESRLSSDGVLSVIAPRKVPPAVEGERKI---PI-AQTGPVRKE---VKDQSN 616
E E+ E+++ + ++V AP KV AVE E+K P+ A+ PV+ E VK++S
Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156
Query: 617 GDKA*VVSSV 646
++ V +V
Sbjct: 157 QEEKEAVVTV 166
>At5g04290.1 68418.m00422 KOW domain-containing transcription factor
family protein
Length = 1493
Score = 27.9 bits (59), Expect = 6.1
Identities = 16/44 (36%), Positives = 20/44 (45%)
Frame = +1
Query: 262 GSRPWLQHQK**G*VPSKPGRPAFRPGRNLC*NGGRLHRG*RQT 393
GS+PW +H + G FR GRN GGR G R +
Sbjct: 1321 GSKPWNEHSGGGRGFGERRGGGGFRGGRNQSGRGGRSFDGGRSS 1364
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 8.1
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -2
Query: 506 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSXRVCLQPRCSRP 363
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,055,349
Number of Sequences: 28952
Number of extensions: 308581
Number of successful extensions: 889
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 853
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 889
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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