BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_E08 (655 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 208 5e-55 SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 204 1e-53 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 30 0.34 SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 28 1.0 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 2.4 SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 26 5.5 SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 5.5 SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 7.2 SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch... 25 7.2 SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces pombe... 25 9.5 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 208 bits (508), Expect = 5e-55 Identities = 103/179 (57%), Positives = 129/179 (72%), Gaps = 2/179 (1%) Frame = +3 Query: 123 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXNVVXARRSSW 302 + +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAY NV+ ARR+SW Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62 Query: 303 RVISSIEQKTE--GSERKQQMAKEYRVKVQKELXXICYDVLGLLDKHLIPKASNPXSKVF 476 R++SSIEQK E G+ + ++ KEYR K+++EL IC D+L +L+KHLIP A++ SKVF Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122 Query: 477 YLKMKGDYYRYLAXVATGETXHSVVQDSXKAYQDAFEISKAKMQPTHPIRLGLALNFSV 653 Y KMKGDYYRYLA A GE S + Y+ A EI+ A++ PTHPIRLGLALNFSV Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSV 181 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 204 bits (497), Expect = 1e-53 Identities = 103/180 (57%), Positives = 129/180 (71%), Gaps = 2/180 (1%) Frame = +3 Query: 120 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXNVVXARRSS 299 MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAY N++ ARR+S Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60 Query: 300 WRVISSIEQKTE--GSERKQQMAKEYRVKVQKELXXICYDVLGLLDKHLIPKASNPXSKV 473 WR+ISSIEQK E G+ R+ + KEYR K++ EL IC+DVL +L+KHLIP A+ SKV Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120 Query: 474 FYLKMKGDYYRYLAXVATGETXHSVVQDSXKAYQDAFEISKAKMQPTHPIRLGLALNFSV 653 FY KMKGDYYRYLA GE S +AY+ A +I+ A++ PT P+RLGLALNFSV Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSV 180 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 29.9 bits (64), Expect = 0.34 Identities = 23/104 (22%), Positives = 41/104 (39%), Gaps = 2/104 (1%) Frame = +3 Query: 102 PLPSSTMSVDKE--ELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXN 275 P+ S TMS E + R + + Y+ MA + E E ++ + LLS Y N Sbjct: 2997 PIMSITMSDSSAYGEELMRERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056 Query: 276 VVXARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVQKELXXIC 407 +R+ + +E+ + EY +++ L C Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100 >SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 285 Score = 28.3 bits (60), Expect = 1.0 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%) Frame = +1 Query: 13 FSAREKLILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLS 177 FS +E ILC L+L+V + ++ S+ + F +PR K N WPN+ S Sbjct: 39 FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98 Query: 178 DM 183 + Sbjct: 99 HL 100 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 27.1 bits (57), Expect = 2.4 Identities = 15/49 (30%), Positives = 21/49 (42%) Frame = -3 Query: 152 TLHQFFLVDGHRGRWKRTNSLMPLSEGEKCI*TSKFKHNISFSLALNGY 6 TL FL DG RWK + S+ K +++ +LNGY Sbjct: 52 TLKDEFLADGLESRWKTNGEKSSSIRYPLVMEFSQLKEDLTNRASLNGY 100 >SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2|Schizosaccharomyces pombe|chr 2|||Manual Length = 659 Score = 25.8 bits (54), Expect = 5.5 Identities = 21/79 (26%), Positives = 35/79 (44%), Gaps = 3/79 (3%) Frame = +1 Query: 310 FPQLNRKPRVQKENSKWQKNIGLKYKRSSXKSATTFWAYLTNT*FLKLVI---XKVKCFT 480 F N + R + +QK + + K+ S + A+ F ++ + +KL+ K CF Sbjct: 75 FIACNGQTRAVQSRGDYQKTLAIALKKFSLEDASKFIVCVSQSSRIKLITEEEFKQICFN 134 Query: 481 SK*KEITTGTLXKWPQEKP 537 S E L P+EKP Sbjct: 135 SSSPE--RDRLIIVPKEKP 151 >SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pombe|chr 2|||Manual Length = 1067 Score = 25.8 bits (54), Expect = 5.5 Identities = 16/46 (34%), Positives = 21/46 (45%) Frame = +1 Query: 25 EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 162 EKL+ C HF +G+ + L RC +T CNVP W Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720 >SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 580 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Frame = +1 Query: 67 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 189 FSP +K + +L LFH + PS+++ V N + SD +T Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201 >SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr 3|||Manual Length = 1374 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 2/31 (6%) Frame = +3 Query: 360 AKEYRVKVQKELXXICYDVL--GLLDKHLIP 446 AK R Q +C D++ GL+DKHL P Sbjct: 590 AKSIRKAKQNAAFIMCLDLIRMGLIDKHLKP 620 >SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 719 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +1 Query: 4 VYPFSAREKLILCLNLL 54 ++PF EK++LCL LL Sbjct: 92 IFPFELCEKIVLCLVLL 108 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,599,214 Number of Sequences: 5004 Number of extensions: 48460 Number of successful extensions: 137 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 132 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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