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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P02_F_E08
         (655 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   208   5e-55
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   204   1e-53
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces...    30   0.34 
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom...    28   1.0  
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom...    27   2.4  
SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2...    26   5.5  
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom...    26   5.5  
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar...    25   7.2  
SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch...    25   7.2  
SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces pombe...    25   9.5  

>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  208 bits (508), Expect = 5e-55
 Identities = 103/179 (57%), Positives = 129/179 (72%), Gaps = 2/179 (1%)
 Frame = +3

Query: 123 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXNVVXARRSSW 302
           +  +E+ V  AKLAEQAERY+ M   MK V  T  EL+ EERNLLSVAY NV+ ARR+SW
Sbjct: 3   TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62

Query: 303 RVISSIEQKTE--GSERKQQMAKEYRVKVQKELXXICYDVLGLLDKHLIPKASNPXSKVF 476
           R++SSIEQK E  G+  + ++ KEYR K+++EL  IC D+L +L+KHLIP A++  SKVF
Sbjct: 63  RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122

Query: 477 YLKMKGDYYRYLAXVATGETXHSVVQDSXKAYQDAFEISKAKMQPTHPIRLGLALNFSV 653
           Y KMKGDYYRYLA  A GE        S + Y+ A EI+ A++ PTHPIRLGLALNFSV
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSV 181


>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  204 bits (497), Expect = 1e-53
 Identities = 103/180 (57%), Positives = 129/180 (71%), Gaps = 2/180 (1%)
 Frame = +3

Query: 120 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXNVVXARRSS 299
           MS  +E  V  AKLAEQAERY++M   MK+V  +  +LS EERNLLSVAY N++ ARR+S
Sbjct: 1   MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60

Query: 300 WRVISSIEQKTE--GSERKQQMAKEYRVKVQKELXXICYDVLGLLDKHLIPKASNPXSKV 473
           WR+ISSIEQK E  G+ R+  + KEYR K++ EL  IC+DVL +L+KHLIP A+   SKV
Sbjct: 61  WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120

Query: 474 FYLKMKGDYYRYLAXVATGETXHSVVQDSXKAYQDAFEISKAKMQPTHPIRLGLALNFSV 653
           FY KMKGDYYRYLA    GE        S +AY+ A +I+ A++ PT P+RLGLALNFSV
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSV 180


>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 3131

 Score = 29.9 bits (64), Expect = 0.34
 Identities = 23/104 (22%), Positives = 41/104 (39%), Gaps = 2/104 (1%)
 Frame = +3

Query: 102  PLPSSTMSVDKE--ELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYXN 275
            P+ S TMS      E + R +     + Y+ MA  + E  E   ++ +    LLS  Y N
Sbjct: 2997 PIMSITMSDSSAYGEELMRERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDN 3056

Query: 276  VVXARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVQKELXXIC 407
                    +R+ + +E+          +  EY   +++ L   C
Sbjct: 3057 EAYQAELFYRLSNCVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100


>SPAC16A10.08c |mug74|SPAC589.01c|sequence
           orphan|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 285

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
 Frame = +1

Query: 13  FSAREKLILC-----LNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLS 177
           FS +E  ILC     L+L+V  +    ++  S+ + F +PR     K   N   WPN+ S
Sbjct: 39  FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98

Query: 178 DM 183
            +
Sbjct: 99  HL 100


>SPBC211.03c |||guanyl-nucleotide exchange
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1462

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 15/49 (30%), Positives = 21/49 (42%)
 Frame = -3

Query: 152 TLHQFFLVDGHRGRWKRTNSLMPLSEGEKCI*TSKFKHNISFSLALNGY 6
           TL   FL DG   RWK              +  S+ K +++   +LNGY
Sbjct: 52  TLKDEFLADGLESRWKTNGEKSSSIRYPLVMEFSQLKEDLTNRASLNGY 100


>SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase
           Byr2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 659

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 21/79 (26%), Positives = 35/79 (44%), Gaps = 3/79 (3%)
 Frame = +1

Query: 310 FPQLNRKPRVQKENSKWQKNIGLKYKRSSXKSATTFWAYLTNT*FLKLVI---XKVKCFT 480
           F   N + R  +    +QK + +  K+ S + A+ F   ++ +  +KL+     K  CF 
Sbjct: 75  FIACNGQTRAVQSRGDYQKTLAIALKKFSLEDASKFIVCVSQSSRIKLITEEEFKQICFN 134

Query: 481 SK*KEITTGTLXKWPQEKP 537
           S   E     L   P+EKP
Sbjct: 135 SSSPE--RDRLIIVPKEKP 151


>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1067

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 16/46 (34%), Positives = 21/46 (45%)
 Frame = +1

Query: 25  EKLILCLNLLVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 162
           EKL+ C       HF    +G+ +  L    RC +T    CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720


>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 580

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +1

Query: 67  FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 189
           FSP +K  + +L LFH  + PS+++    V N  +  SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201


>SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1374

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 2/31 (6%)
 Frame = +3

Query: 360 AKEYRVKVQKELXXICYDVL--GLLDKHLIP 446
           AK  R   Q     +C D++  GL+DKHL P
Sbjct: 590 AKSIRKAKQNAAFIMCLDLIRMGLIDKHLKP 620


>SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 719

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 9/17 (52%), Positives = 13/17 (76%)
 Frame = +1

Query: 4   VYPFSAREKLILCLNLL 54
           ++PF   EK++LCL LL
Sbjct: 92  IFPFELCEKIVLCLVLL 108


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,599,214
Number of Sequences: 5004
Number of extensions: 48460
Number of successful extensions: 137
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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