Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_E07
(597 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) / putre... 136 1e-32
At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) / putre... 134 6e-32
At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) / putre... 134 6e-32
At5g53120.3 68418.m06603 spermidine synthase, putative / putresc... 127 6e-30
At5g53120.2 68418.m06602 spermidine synthase, putative / putresc... 127 6e-30
At5g53120.1 68418.m06601 spermidine synthase, putative / putresc... 127 6e-30
At5g19530.1 68418.m02326 spermine/spermidine synthase family pro... 91 7e-19
At2g04920.1 68415.m00513 F-box family protein (FBX9) identical t... 31 0.58
At3g04350.1 68416.m00460 expressed protein 29 3.1
At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family... 28 4.1
At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4... 28 5.4
At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyc... 27 9.5
>At1g70310.1 68414.m08089 spermidine synthase 2 (SPDSYN2) /
putrescine aminopropyltransferase 2 identical to
SP|O48661 Spermidine synthase 2 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 2) (SPDSY 2)
{Arabidopsis thaliana}
Length = 340
Score = 136 bits (328), Expect = 1e-32
Identities = 63/142 (44%), Positives = 90/142 (63%)
Frame = +3
Query: 168 MDKLKTNWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCT 347
M + WF+E MWPG S +V+++L KS YQ++ VF + + GKVLVLDG+IQ T
Sbjct: 44 MSSIIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLT 103
Query: 348 XKDXFSYQEMISFLPLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIEL 527
+D +YQEMI+ LPLC NP VL+ V REVA+H V+ I + EID V+++
Sbjct: 104 ERDECAYQEMITHLPLCSISNPKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDV 163
Query: 528 SKRYLPFMAVGFXSPKLNSSCG 593
+K+Y P +AVG+ P++N G
Sbjct: 164 AKQYFPNVAVGYEDPRVNLIIG 185
>At1g23820.2 68414.m03004 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 283
Score = 134 bits (323), Expect = 6e-32
Identities = 61/135 (45%), Positives = 88/135 (65%)
Frame = +3
Query: 189 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFSY 368
WF+E MWPG S +V++VL KS YQ++ VF + + GKVLVLDG+IQ T +D +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 369 QEMISFLPLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPF 548
QEMI+ LPLC NP VL+ V REVA+H ++ I + EID V+++SK++ P
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPD 166
Query: 549 MAVGFXSPKLNSSCG 593
+A+G+ P++N G
Sbjct: 167 VAIGYEDPRVNLVIG 181
>At1g23820.1 68414.m03005 spermidine synthase 1 (SPDSYN1) /
putrescine aminopropyltransferase 1 identical to
SP|Q9ZUB3 Spermidine synthase 1 (EC 2.5.1.16)
(Putrescine aminopropyltransferase 1) (SPDSY 1)
{Arabidopsis thaliana}
Length = 334
Score = 134 bits (323), Expect = 6e-32
Identities = 61/135 (45%), Positives = 88/135 (65%)
Frame = +3
Query: 189 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFSY 368
WF+E MWPG S +V++VL KS YQ++ VF + + GKVLVLDG+IQ T +D +Y
Sbjct: 47 WFSEMSPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 106
Query: 369 QEMISFLPLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPF 548
QEMI+ LPLC NP VL+ V REVA+H ++ I + EID V+++SK++ P
Sbjct: 107 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPD 166
Query: 549 MAVGFXSPKLNSSCG 593
+A+G+ P++N G
Sbjct: 167 VAIGYEDPRVNLVIG 181
>At5g53120.3 68418.m06603 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 127 bits (306), Expect = 6e-30
Identities = 59/128 (46%), Positives = 85/128 (66%)
Frame = +3
Query: 210 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFSYQEMISFL 389
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T KD +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 390 PLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMAVGFXS 569
PLC +P NVL+ V RE+++H V+ I + EID VI++SK++ P +AVGF
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDD 196
Query: 570 PKLNSSCG 593
P++ G
Sbjct: 197 PRVQLHIG 204
>At5g53120.2 68418.m06602 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 127 bits (306), Expect = 6e-30
Identities = 59/128 (46%), Positives = 85/128 (66%)
Frame = +3
Query: 210 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFSYQEMISFL 389
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T KD +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 390 PLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMAVGFXS 569
PLC +P NVL+ V RE+++H V+ I + EID VI++SK++ P +AVGF
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDD 196
Query: 570 PKLNSSCG 593
P++ G
Sbjct: 197 PRVQLHIG 204
>At5g53120.1 68418.m06601 spermidine synthase, putative / putrescine
aminopropyltransferase, putative similar to SP|O82147
Spermidine synthase (EC 2.5.1.16) (Putrescine
aminopropyltransferase) (SPDSY) {Coffea arabica};
contains Pfam profile PF01564: Spermine/spermidine
synthase
Length = 359
Score = 127 bits (306), Expect = 6e-30
Identities = 59/128 (46%), Positives = 85/128 (66%)
Frame = +3
Query: 210 MWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFSYQEMISFL 389
MWPG S +V++VL +KS +Q + VF++ + GKVLVLDGI+Q T KD +YQEMI+ L
Sbjct: 77 MWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHL 136
Query: 390 PLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIELSKRYLPFMAVGFXS 569
PLC +P NVL+ V RE+++H V+ I + EID VI++SK++ P +AVGF
Sbjct: 137 PLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDD 196
Query: 570 PKLNSSCG 593
P++ G
Sbjct: 197 PRVQLHIG 204
>At5g19530.1 68418.m02326 spermine/spermidine synthase family
protein similar to SP|P09158 Spermidine synthase (EC
2.5.1.16) (Putrescine aminopropyltransferase)
{Escherichia coli}; contains Pfam profile PF01564:
Spermine/spermidine synthase
Length = 339
Score = 90.6 bits (215), Expect = 7e-19
Identities = 46/131 (35%), Positives = 70/131 (53%)
Frame = +3
Query: 186 NWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFS 365
+W+ E+ D +SF + VLH S+YQ+I + DT GKVLV+DG +Q +D F
Sbjct: 34 HWYEETID--DDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDGKMQSAERDEFI 91
Query: 366 YQEMISFLPLCCHKNPXNVLIXXXXXXXVAREVAKHPQVKHIVLVEIDDRVIELSKRYLP 545
Y E + L H NP V I ARE+ KH ++ +V+ +ID V++ +R+L
Sbjct: 92 YHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQEVVDFCRRFLT 151
Query: 546 FMAVGFXSPKL 578
+ F + KL
Sbjct: 152 VNSDAFCNKKL 162
>At2g04920.1 68415.m00513 F-box family protein (FBX9) identical to
F-box protein family, AtFBX9 (GI:20197985) [Arabidopsis
thaliana]; contains F-box domain PF:00646; contains
TIGRFAM TIGR01640 : F-box protein interaction domain
Length = 376
Score = 31.1 bits (67), Expect = 0.58
Identities = 17/42 (40%), Positives = 23/42 (54%)
Frame = +3
Query: 231 SFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKD 356
S E K VL + S Y + QV+ + KV DG++ CT KD
Sbjct: 75 SIEFKSVLSLKDSHYNSEQVY----IAKVFHCDGLLLCTTKD 112
>At3g04350.1 68416.m00460 expressed protein
Length = 567
Score = 28.7 bits (61), Expect = 3.1
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -2
Query: 353 FXSALYDSVQDKNLPQTGCIKNLD 282
F YD ++ +P GC+KNLD
Sbjct: 227 FFCCTYDLSSERTVPDIGCLKNLD 250
>At4g11800.1 68417.m01879 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 1012
Score = 28.3 bits (60), Expect = 4.1
Identities = 17/68 (25%), Positives = 29/68 (42%)
Frame = +3
Query: 189 WFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQCTXKDXFSY 368
WF D GG S+ V ++L + F + G VL++ G + F+Y
Sbjct: 369 WFDFMADTGDGGNSSYSVAKLLAQPSLRVPVANNFISLPRGNVLLIGGDLAYPNPSSFTY 428
Query: 369 QEMISFLP 392
++ + F P
Sbjct: 429 EKRL-FCP 435
>At4g14580.1 68417.m02244 CBL-interacting protein kinase 4 (CIPK4)
identical to CBL-interacting protein kinase 4
[Arabidopsis thaliana] gi|13249503|gb|AAG01367;
identical to cDNA calcineurin B-like (CBL) interacting
protein kinase 4 (CIPK4) GI:13249502
Length = 426
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/53 (30%), Positives = 23/53 (43%)
Frame = +3
Query: 183 TNWFTESCDMWPGGTFSFEVKEVLHTEKSKYQNIQVFDTTSLGKVLVLDGIIQ 341
T WF +S ++ + FE+ L E I FD SL L L G+ +
Sbjct: 274 TVWFQKSLEISEFQSSVFELDRFLEKEAKSSNAITAFDLISLSSGLDLSGLFE 326
>At5g11300.1 68418.m01319 cyclin, putative (CYC3b) similar to cyclin
3a [Arabidopsis thaliana] GI:509425; contains Pfam
profiles PF00134: Cyclin, N-terminal domain, PF02984:
Cyclin, C-terminal domain; identical to cDNA cyc3b mRNA
for cyclin 3b protein GI:728520
Length = 436
Score = 27.1 bits (57), Expect = 9.5
Identities = 17/60 (28%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +2
Query: 41 FIFRARNTLFEREIVKTETXCQNLFNFRLQLPTWY*LLKQ-EKXGQTKNQLVYGIMRYVA 217
F F NT E++ E N +FRL +PT L++ K Q ++ + + Y+A
Sbjct: 273 FCFITANTYTRPEVLSMEIQILNFVHFRLSVPTTKTFLRRFIKAAQASYKVPFIELEYLA 332
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,822,467
Number of Sequences: 28952
Number of extensions: 219008
Number of successful extensions: 517
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 511
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 517
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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