BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_C17 (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 338 3e-94 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 338 4e-94 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 338 4e-94 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 188 8e-49 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 184 1e-47 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 105 4e-24 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 53 3e-08 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 42 6e-05 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 41 2e-04 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 39 7e-04 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 39 7e-04 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 38 0.001 SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 30 0.34 SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 1.8 SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 27 2.4 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 27 2.4 SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 25 7.2 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 338 bits (832), Expect = 3e-94 Identities = 160/196 (81%), Positives = 175/196 (89%) Frame = +1 Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVL 243 MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTI KFEKEA E+GKGSFKYAWVL Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60 Query: 244 DKLKAERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGT 423 DKLKAERERGITIDIALWKFET KY VT+IDAP HR FIKNMITGTSQADCAVLI+ GT Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAVLIIGGGT 120 Query: 424 GEFEXGISXNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKK 603 GEFE GIS +GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK Sbjct: 121 GEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKK 178 Query: 604 IGYNPAAVAFVPISGW 651 +G+NP V FVP+SG+ Sbjct: 179 VGFNPKTVPFVPVSGF 194 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 338 bits (831), Expect = 4e-94 Identities = 159/196 (81%), Positives = 175/196 (89%) Frame = +1 Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVL 243 MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTI KFEKEA E+GKGSFKYAWVL Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60 Query: 244 DKLKAERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGT 423 DKLKAERERGITIDIALWKFET KY VT+IDAP HR FIKNMITGTSQADCA+LI+ GT Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGT 120 Query: 424 GEFEXGISXNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKK 603 GEFE GIS +GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK Sbjct: 121 GEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKK 178 Query: 604 IGYNPAAVAFVPISGW 651 +G+NP V FVP+SG+ Sbjct: 179 VGFNPKTVPFVPVSGF 194 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 338 bits (831), Expect = 4e-94 Identities = 159/196 (81%), Positives = 175/196 (89%) Frame = +1 Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVL 243 MGKEK HIN+VVIGHVDSGKSTTTGHLIYKCGGIDKRTI KFEKEA E+GKGSFKYAWVL Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVL 60 Query: 244 DKLKAERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGT 423 DKLKAERERGITIDIALWKFET KY VT+IDAP HR FIKNMITGTSQADCA+LI+ GT Sbjct: 61 DKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGT 120 Query: 424 GEFEXGISXNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKK 603 GEFE GIS +GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++IKK Sbjct: 121 GEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKK 178 Query: 604 IGYNPAAVAFVPISGW 651 +G+NP V FVP+SG+ Sbjct: 179 VGFNPKTVPFVPVSGF 194 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 188 bits (457), Expect = 8e-49 Identities = 87/194 (44%), Positives = 128/194 (65%), Gaps = 2/194 (1%) Frame = +1 Query: 76 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLK 255 K H+NIV IGHVD+GKST G++++ G +DKRT+ K E+EA E GK S+ +W LD Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNILFLTGMVDKRTMEKIEREAKEAGKESWYLSWALDSTS 295 Query: 256 AERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTGEFE 435 ERE+G T+++ FET +++DAP H+ ++ NMI G SQAD VL++ A GEFE Sbjct: 296 EEREKGKTVEVGRAYFETEHRRFSLLDAPGHKGYVTNMINGASQADIGVLVISARRGEFE 355 Query: 436 XGISXNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYIKKI-GY 612 G GQTREHA+LA T G+ L+V +NKMD +SE R++E ++S +++++ GY Sbjct: 356 AGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRRVAGY 415 Query: 613 NPAA-VAFVPISGW 651 N V ++P+S + Sbjct: 416 NSKTDVKYMPVSAY 429 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 184 bits (448), Expect = 1e-47 Identities = 93/191 (48%), Positives = 122/191 (63%), Gaps = 1/191 (0%) Frame = +1 Query: 76 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLK 255 K +++VV GHVDSGKST G ++++ G I+ R++ K EA GKGSF YAW+LD + Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTE 234 Query: 256 AERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTGEFE 435 ER RG+T+D+A FE+ K I DAP HR FI MI G S AD AVL+V + FE Sbjct: 235 EERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFE 294 Query: 436 XGISXNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSY-IKKIGY 612 G NGQTREHA L LG+ +++V VNK+D +SE RF+EI VS + IK +G+ Sbjct: 295 RGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGF 352 Query: 613 NPAAVAFVPIS 645 + V FVPIS Sbjct: 353 KTSNVHFVPIS 363 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 105 bits (253), Expect = 4e-24 Identities = 67/175 (38%), Positives = 94/175 (53%) Frame = +1 Query: 70 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDK 249 ++K H+NI IGHVD GK+T T I KC ++G+ SF +DK Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLTA-AITKC--------------LSDLGQASFMDYSQIDK 93 Query: 250 LKAERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTGE 429 E+ RGITI A ++ET + +D P H +IKNMITG + D A+++V A G+ Sbjct: 94 APEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAATMDGAIIVVSATDGQ 153 Query: 430 FEXGISXNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSY 594 QTREH LLA +GVKQ++V +NK+D EP E E+ + +S Y Sbjct: 154 MP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 53.2 bits (122), Expect = 3e-08 Identities = 45/148 (30%), Positives = 65/148 (43%) Frame = +1 Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERE 267 N+ +I H+D+GK+T T ++Y G F + G V+D L AER+ Sbjct: 30 NVGIIAHIDAGKTTLTEKMLYYGG---------FTSHFGNVDTGDT----VMDYLPAERQ 76 Query: 268 RGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTGEFEXGIS 447 RGITI+ A F + +ID P H F + + D AV I+ G Sbjct: 77 RGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAG------- 129 Query: 448 XNGQTREHALLAFTLGVKQLIVGVNKMD 531 QT+ A G+ ++I VNKMD Sbjct: 130 VEAQTKVVWKQATKRGIPKVIF-VNKMD 156 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 42.3 bits (95), Expect = 6e-05 Identities = 40/152 (26%), Positives = 67/152 (44%), Gaps = 4/152 (2%) Frame = +1 Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERE 267 N VI H+D GKST + ++ G I++ + F LDKL+ ER Sbjct: 60 NWAVIAHIDHGKSTLSDCILKLTGVINEHNF-----------RNQF-----LDKLEVERR 103 Query: 268 RGITIDI----ALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTGEFE 435 RGIT+ ++ + Y + +ID P H F ++ + + +L+V A G Sbjct: 104 RGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQG--- 160 Query: 436 XGISXNGQTREHALLAFTLGVKQLIVGVNKMD 531 QT + +AF+ + +I +NK+D Sbjct: 161 ----IQAQTLSNFYMAFSQNL-VIIPVLNKVD 187 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 40.7 bits (91), Expect = 2e-04 Identities = 43/147 (29%), Positives = 64/147 (43%), Gaps = 19/147 (12%) Frame = +1 Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERE 267 NI + H+DSGK+T T ++Y G I K+ E+ +G +D ++ ERE Sbjct: 61 NIGISAHIDSGKTTFTERVLYYTGRI---------KDIHEV-RGKDNVGAKMDFMELERE 110 Query: 268 RGITIDIA----LWK---------------FETXKYYVTIIDAPXHRXFIKNMITGTSQA 390 +GITI A W+ FE Y + IID P H F + Sbjct: 111 KGITIQSAATHCTWERTVDQIEANEKQKTDFE-KSYNINIIDTPGHIDFTIEVERALRVL 169 Query: 391 DCAVLIVXAGTGEFEXGISXNGQTREH 471 D AVL++ A +G I+ + Q R + Sbjct: 170 DGAVLVLCAVSGVQSQTITVDRQMRRY 196 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 38.7 bits (86), Expect = 7e-04 Identities = 36/119 (30%), Positives = 52/119 (43%), Gaps = 3/119 (2%) Frame = +1 Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGID--KRTIXKF-EKEAXEMGKGSFKYA 234 MGK N+ VI HVD GKST T L+ K G I K +F + A E +G + Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKS 72 Query: 235 WVLDKLKAERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIV 411 + L AE D+ + + V +ID+P H F + D A+++V Sbjct: 73 TAIS-LFAEMTDDDMKDMKE-PADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 38.7 bits (86), Expect = 7e-04 Identities = 36/119 (30%), Positives = 52/119 (43%), Gaps = 3/119 (2%) Frame = +1 Query: 64 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGID--KRTIXKF-EKEAXEMGKGSFKYA 234 MGK N+ VI HVD GKST T L+ K G I K +F + A E +G + Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKAGDARFMDTRADEQERGVTIKS 72 Query: 235 WVLDKLKAERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIV 411 + L AE D+ + + V +ID+P H F + D A+++V Sbjct: 73 TAIS-LFAEMTDDDMKDMKE-PADGTDFLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 38.3 bits (85), Expect = 0.001 Identities = 23/93 (24%), Positives = 42/93 (45%) Frame = +1 Query: 319 YVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTGEFEXGISXNGQTREHALLAFTLGV 498 +V+ +D P H + M+ G + D A+L++ AG QT EH + + Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLI-AGNES-----CPQPQTSEHLAAIEIMQL 162 Query: 499 KQLIVGVNKMDSTEPPYSEPRFEEIXKEVSSYI 597 K +I+ NK+D +E ++ I K + + Sbjct: 163 KHIIILQNKVDLIRESAAEEHYQSILKFIKGTV 195 >SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 29.9 bits (64), Expect = 0.34 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = -1 Query: 281 IVIPRSRSAFSLSNTQAYLKDPLPISWASFSNXSMVRLSIPPH 153 I IP+ A S+ N++ + +D LP + S V+L +P H Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213 >SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharomyces pombe|chr 3|||Manual Length = 642 Score = 27.5 bits (58), Expect = 1.8 Identities = 16/44 (36%), Positives = 22/44 (50%) Frame = -1 Query: 305 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSNXSMV 174 S F IS+ +P FSL+ ++ K LPI W S + S V Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446 >SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1079 Score = 27.1 bits (57), Expect = 2.4 Identities = 24/105 (22%), Positives = 44/105 (41%) Frame = +1 Query: 97 VIGHVDSGKSTTTGHLIYKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGI 276 ++GHVD+GK+ +L + + + ++ +G F + K K ++G Sbjct: 489 ILGHVDTGKTKLLDNL--RRSNVQEGEAGGITQQ---IGATYFPIESIKQKTKVVNKKG- 542 Query: 277 TIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIV 411 K + + IID P H F GTS + A+L++ Sbjct: 543 -------KLQYNIPGLLIIDTPGHESFTNLRSRGTSLCNIAILVI 580 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 27.1 bits (57), Expect = 2.4 Identities = 28/116 (24%), Positives = 46/116 (39%), Gaps = 3/116 (2%) Frame = +1 Query: 88 NIVVIGHVDSGKSTTTGHLIYKCGGIDKR---TIXKFEKEAXEMGKGSFKYAWVLDKLKA 258 N ++ HVD GK+T L+ G I + T+ + E+ +G + K A Sbjct: 21 NFTLLAHVDHGKTTLADSLLASNGIISSKLAGTVRFLDFREDEITRG------ITMKSSA 74 Query: 259 ERERGITIDIALWKFETXKYYVTIIDAPXHRXFIKNMITGTSQADCAVLIVXAGTG 426 I K Y + +ID+P H F + + + D A ++V A G Sbjct: 75 ISLFFKVISQNDEKRVEKDYLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130 >SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 367 Score = 25.4 bits (53), Expect = 7.2 Identities = 9/30 (30%), Positives = 18/30 (60%) Frame = +2 Query: 548 TVSPDLRKSXRKYPHTSRRLATTQLLSLSC 637 + +PD+ ++PHT +R +T + L + C Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,396,736 Number of Sequences: 5004 Number of extensions: 42643 Number of successful extensions: 145 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 137 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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