Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_B18
(653 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 202 4e-53
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 100 2e-22
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 29 0.44
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.4
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 2.4
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 5.5
SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma... 25 9.5
SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 25 9.5
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 25 9.5
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 202 bits (493), Expect = 4e-53
Identities = 89/157 (56%), Positives = 122/157 (77%), Gaps = 1/157 (0%)
Frame = +3
Query: 183 IKFLEVIKPFCSILPEIAKPERKIQFRXKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 362
++FL+++KPF LPEIA PERK+ F+ K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 363 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAXKLFGM 539
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ K +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Query: 540 VITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVA 650
+I GQA YV+TGMYG P ++GAG+CLL+I+QL A
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAA 160
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 100 bits (240), Expect = 2e-22
Identities = 52/159 (32%), Positives = 90/159 (56%), Gaps = 1/159 (0%)
Frame = +3
Query: 180 GIKFLEVIKPFCSILPEIAKPERKIQFRXKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 359
G +F+ IKP S+LPE+ P+ ++ K+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 360 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAXKLFG 536
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF A K+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122
Query: 537 MVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAG 653
++ + A+ YV +G YG S++G +++I+Q+F+ G
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPG 161
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.5 bits (63), Expect = 0.44
Identities = 23/65 (35%), Positives = 29/65 (44%), Gaps = 4/65 (6%)
Frame = -2
Query: 523 CAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ*KG 356
CA +NKAL G+ TS A C PD I + S V PL + RI ++ G
Sbjct: 106 CAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRASG 162
Query: 355 SALSD 341
L D
Sbjct: 163 RVLVD 167
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.1 bits (57), Expect = 2.4
Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = -1
Query: 638 LYDNEEAHSGTNFTGFTIHSSHYINN-SLTNSNHHT 534
+Y + E H+G G +HSS NN S T+SN T
Sbjct: 150 VYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +3
Query: 183 IKFLEVI-KPFCSILPEIAKPERKIQFRXKVLWTAITLFIFLVCCQIPLFGIMS 341
I+FL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 5.5
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -2
Query: 232 ISGNILQNGLITSKNFIPILIYC 164
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
>SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 899
Score = 25.0 bits (52), Expect = 9.5
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = +1
Query: 61 LFSVKKSYWSVINEQKLNGY*RIDQLI 141
L S+ K+ W+V++E++ GY + +LI
Sbjct: 3 LQSLVKALWNVLHEEESEGYPDLTELI 29
>SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 557
Score = 25.0 bits (52), Expect = 9.5
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2
Query: 445 ISPDVTIGEIPSSINVPLFDA 383
IS ++T GE+PS NVP +A
Sbjct: 414 ISANMTTGEMPSMTNVPAGNA 434
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 25.0 bits (52), Expect = 9.5
Identities = 15/56 (26%), Positives = 28/56 (50%)
Frame = -2
Query: 532 NNXCAPLNKALSFGVSPTSMILAPASNCMISPDVTIGEIPSSINVPLFDARITRIQ 365
N C +K + P ++I +P+S S ++ + PSS+NV + R+ +Q
Sbjct: 368 NGLCRNDSKCRFLMILPETIIKSPSSFIGDSYNIANIDPPSSMNVQSSEFRVGEVQ 423
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,647,753
Number of Sequences: 5004
Number of extensions: 52356
Number of successful extensions: 142
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 140
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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