BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_B16 (654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 275 4e-75 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 153 2e-38 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 152 4e-38 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 125 5e-30 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 81 1e-16 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 79 4e-16 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 75 1e-14 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 55 1e-08 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.083 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 29 0.77 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.77 SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 4.1 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 4.1 SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 26 5.5 SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosa... 25 7.2 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 275 bits (674), Expect = 4e-75 Identities = 126/141 (89%), Positives = 131/141 (92%) Frame = +3 Query: 231 PAIRAVMVGMGQKDSYVXDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 410 P +MVGMGQKDSYV DEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97 Query: 411 PEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAXGRTTGIVLDSGD 590 PEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSLYA GRTTGIVLDSGD Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGD 157 Query: 591 GVSHTVPIYEGYALPHAILRL 653 GV+HTVPIYEGYALPHAI+RL Sbjct: 158 GVTHTVPIYEGYALPHAIMRL 178 Score = 86.6 bits (205), Expect = 3e-18 Identities = 37/41 (90%), Positives = 40/41 (97%) Frame = +2 Query: 122 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 244 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 153 bits (371), Expect = 2e-38 Identities = 69/134 (51%), Positives = 97/134 (72%), Gaps = 1/134 (0%) Frame = +3 Query: 246 VMVGMGQKDSYVXDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEH 422 VM QKD +V EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEH Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106 Query: 423 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAXGRTTGIVLDSGDGVSH 602 P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYA RTTGIVL+ GDG++H Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166 Query: 603 TVPIYEGYALPHAI 644 +VPIY+G+++P AI Sbjct: 167 SVPIYDGFSIPSAI 180 Score = 52.4 bits (120), Expect = 5e-08 Identities = 20/34 (58%), Positives = 27/34 (79%) Frame = +2 Query: 140 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 241 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 152 bits (369), Expect = 4e-38 Identities = 66/129 (51%), Positives = 96/129 (74%) Frame = +3 Query: 267 KDSYVXDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 446 KD V DEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109 Query: 447 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAXGRTTGIVLDSGDGVSHTVPIYEGY 626 +NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169 Query: 627 ALPHAILRL 653 L H + RL Sbjct: 170 VLNHLVGRL 178 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +2 Query: 128 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 232 E A +V+DNG+G K G+A D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 125 bits (302), Expect = 5e-30 Identities = 60/134 (44%), Positives = 83/134 (61%), Gaps = 9/134 (6%) Frame = +3 Query: 270 DSYVXDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 446 D ++ ++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132 Query: 447 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAXGRT--------TGIVLDSGDGVSH 602 LNP NRE +IMFE+FN +Y+A+QAVL+L A + TG V+DSGDGV+H Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTH 192 Query: 603 TVPIYEGYALPHAI 644 +P+ EGY + +I Sbjct: 193 IIPVAEGYVIGSSI 206 Score = 40.7 bits (91), Expect = 2e-04 Identities = 14/28 (50%), Positives = 23/28 (82%) Frame = +2 Query: 140 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 223 +++DNG+G K G+AG+DAP VFP+++ Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 81.4 bits (192), Expect = 1e-16 Identities = 39/107 (36%), Positives = 58/107 (54%) Frame = +3 Query: 312 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 491 + +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122 Query: 492 ETFNTPAMYVAIQAVLSLYAXGRTTGIVLDSGDGVSHTVPIYEGYAL 632 E+ PA Y+A Q + +A G+ T ++D G S IY+G+ L Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 24/34 (70%) Frame = +2 Query: 125 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 226 +EV+A+V+D GS + GF+G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 79.4 bits (187), Expect = 4e-16 Identities = 36/101 (35%), Positives = 60/101 (59%), Gaps = 2/101 (1%) Frame = +3 Query: 327 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNT 506 P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE +N Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFELYNA 144 Query: 507 PAMYVAIQAVLSLYAXGR--TTGIVLDSGDGVSHTVPIYEG 623 P++ I + S Y + ++GIVL+ G+ SH +P+ G Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNG 185 Score = 35.9 bits (79), Expect = 0.005 Identities = 15/32 (46%), Positives = 22/32 (68%) Frame = +2 Query: 140 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 235 LV+DNGS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 74.5 bits (175), Expect = 1e-14 Identities = 37/142 (26%), Positives = 72/142 (50%), Gaps = 2/142 (1%) Frame = +3 Query: 231 PAIRAVMVGMGQKDSYVXDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 404 P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+ Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96 Query: 405 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYAXGRTTGIVLDS 584 V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A ++T +++D Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDI 156 Query: 585 GDGVSHTVPIYEGYALPHAILR 650 G + P+ +G + I + Sbjct: 157 GSDNASVTPVVDGLIIRKGIFK 178 Score = 36.7 bits (81), Expect = 0.003 Identities = 14/34 (41%), Positives = 23/34 (67%) Frame = +2 Query: 125 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 226 EE+ +LV+D GS + G+AG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 54.8 bits (126), Expect = 1e-08 Identities = 32/103 (31%), Positives = 49/103 (47%), Gaps = 4/103 (3%) Frame = +3 Query: 327 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 494 PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171 Query: 495 TFNTPAMYVAIQAVLSLYAXGRTTGIVLDSGDGVSHTVPIYEG 623 P +A + ++ LYA G G+V+D G + PI +G Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDG 214 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 31.9 bits (69), Expect = 0.083 Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Frame = +3 Query: 387 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 530 +Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.7 bits (61), Expect = 0.77 Identities = 12/35 (34%), Positives = 16/35 (45%) Frame = +1 Query: 532 PCSRCTRXVVPPVSCWTPATVSPTPCPSTRDTHSP 636 PC+ + PPV+C TP P C + H P Sbjct: 621 PCTCGRTRLYPPVACGTPIPDCPYLCVLPKSCHHP 655 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 28.7 bits (61), Expect = 0.77 Identities = 16/45 (35%), Positives = 19/45 (42%) Frame = +1 Query: 511 PCTSPSKPCSRCTRXVVPPVSCWTPATVSPTPCPSTRDTHSPTPS 645 P T+P P T V P S P+ P P PS+ P PS Sbjct: 1037 PSTAPPVPIPTSTPPV-PKSSSGAPSAPPPVPAPSSEIPSIPAPS 1080 >SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 26.2 bits (55), Expect = 4.1 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 429 LLTEAPLNPKANREKMTQIMFETFN 503 +L APL+ + +KM +++FET+N Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 26.2 bits (55), Expect = 4.1 Identities = 18/54 (33%), Positives = 23/54 (42%), Gaps = 1/54 (1%) Frame = +1 Query: 487 CSKHSTRPPCTSPSKPCSRCTRXVVPPVS-CWTPATVSPTPCPSTRDTHSPTPS 645 C+ ++ PP T S S VPP S T + PT ST SP P+ Sbjct: 172 CTTSTSIPP-TGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPT 224 Score = 25.0 bits (52), Expect = 9.5 Identities = 17/54 (31%), Positives = 23/54 (42%), Gaps = 1/54 (1%) Frame = +1 Query: 487 CSKHSTRPPCTSPSKPCSRCTRXVVPPVS-CWTPATVSPTPCPSTRDTHSPTPS 645 C+ ++ PP T S S VPP S T + PT ST +P P+ Sbjct: 115 CTTSTSIPP-TGGSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSNPLPT 167 >SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1016 Score = 25.8 bits (54), Expect = 5.5 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = +1 Query: 514 CTSPSKPCSRCTRXVVPPVSCWTPATVSPTPCPSTRDTHSPTPS 645 C +PS+ C + PV + T S TP P+T + +PTP+ Sbjct: 825 CYNPSQ--YGCDNGALGPVQS-SSTTSSITPTPTTTSSITPTPT 865 >SPAC1D4.14 |tho2|SPAC22F3.14c|THO complex subunit Tho2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1628 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/50 (30%), Positives = 27/50 (54%) Frame = +2 Query: 443 SPQPQGQQREDDPDHVRNIQHARHVRRHPSRALAVRVRSYHRYRAGLRRR 592 +PQ + E++ D++R++ +RH RR P +A + R H + R R Sbjct: 1428 TPQSRSFTNENN-DNLRSV--SRHTRREPQQAQNLNARREHESQKSDRWR 1474 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,872,641 Number of Sequences: 5004 Number of extensions: 60878 Number of successful extensions: 217 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 179 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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