Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_B06
(656 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE... 75 3e-14
At1g02010.1 68414.m00119 cytokinesis-related Sec1 protein, putat... 74 7e-14
At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putat... 73 2e-13
At5g46540.1 68418.m05730 ABC transporter family protein contains... 29 2.1
At4g23330.1 68417.m03362 eukaryotic translation initiation facto... 29 2.1
At4g28300.2 68417.m04053 hydroxyproline-rich glycoprotein family... 28 4.8
At4g28300.1 68417.m04052 hydroxyproline-rich glycoprotein family... 28 4.8
At1g30160.1 68414.m03687 expressed protein contains Pfam profile... 28 6.3
At4g33630.2 68417.m04778 expressed protein 27 8.3
At4g33630.1 68417.m04777 expressed protein 27 8.3
At3g03530.1 68416.m00353 phosphoesterase family protein low simi... 27 8.3
At1g56690.1 68414.m06520 pentatricopeptide (PPR) repeat-containi... 27 8.3
>At1g12360.1 68414.m01428 cytokinesis-related Sec1 protein (KEULE)
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 666
Score = 75.4 bits (177), Expect = 3e-14
Identities = 39/117 (33%), Positives = 65/117 (55%), Gaps = 11/117 (9%)
Frame = +1
Query: 280 LLHELTLQAMAYDLLPIENDVYKY-----DASQGQLKEVLLDENDDLWVELRHQHIAVVS 444
++HE T AM +DLL +E + Y + Q + K+VLL+E+D +W+ELRH HIA S
Sbjct: 268 VIHEWTYDAMCHDLLNMEGNKYVHVIPSKSGGQPEKKDVLLEEHDPIWLELRHAHIADAS 327
Query: 445 TAVTKNLXKFTESKRMG------GGDKQSMRDLSQMIKKMPQYQKELSKYATHLRLA 597
+ + F + G + S RDL +M++ +PQY +++ K + H+ +A
Sbjct: 328 ERLHDKMTNFLSKNKAAQLQGKRDGAELSTRDLQKMVQALPQYSEQIDKLSLHVEIA 384
Score = 39.1 bits (87), Expect = 0.003
Identities = 28/86 (32%), Positives = 45/86 (52%), Gaps = 9/86 (10%)
Frame = +3
Query: 48 NANMERIAEQIATLCATLGEYPSVRYRS----DWERNVELAQLIQQKLDA-----YKADE 200
+A + +A +IAT+ A+L E+P+VRYR+ D L LI KL A +
Sbjct: 182 DACLNVMASRIATVFASLREFPAVRYRAAKSLDASTMTTLRDLIPTKLAAGIWNCLAKHK 241
Query: 201 PTMGEGPEKARSQLLVLDRGFDCVSP 278
++ P+ +LL+LDR D ++P
Sbjct: 242 QSIENFPQTETCELLILDRSIDQIAP 267
>At1g02010.1 68414.m00119 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family; non-consensus GC
donor splice site at exon boundary 46833
Length = 676
Score = 74.1 bits (174), Expect = 7e-14
Identities = 39/116 (33%), Positives = 68/116 (58%), Gaps = 10/116 (8%)
Frame = +1
Query: 280 LLHELTLQAMAYDLLPIEND--VYKYDASQG---QLKEVLLDENDDLWVELRHQHIAVVS 444
++HE T AM +DLL +E + V + + G + KE++L+++D +W+ELRH HIA S
Sbjct: 267 IIHEWTYDAMCHDLLDMEGNKHVIEVPSKTGGPPEKKEIVLEDHDPVWLELRHTHIADAS 326
Query: 445 TAVTKNLXKFTESK-----RMGGGDKQSMRDLSQMIKKMPQYQKELSKYATHLRLA 597
+ + + F R G + S RDL ++++ +PQY +++ K +TH+ LA
Sbjct: 327 ERLHEKMTNFASKNKAAQMRSRDGSELSTRDLQKIVQALPQYGEQVDKLSTHVELA 382
Score = 34.7 bits (76), Expect = 0.055
Identities = 27/77 (35%), Positives = 42/77 (54%), Gaps = 6/77 (7%)
Frame = +3
Query: 66 IAEQIATLCATLGEYPSVRYR---SDWERNV---ELAQLIQQKLDAYKADEPTMGEGPEK 227
+A +IAT+ A+L E P VRYR S R++ +LA I + YKA + P+
Sbjct: 194 MATRIATVFASLKELPFVRYRAAKSTASRDLVPSKLAAAIWDCISKYKA----IPNFPQT 249
Query: 228 ARSQLLVLDRGFDCVSP 278
+LL++DR D ++P
Sbjct: 250 ETCELLIVDRSVDQIAP 266
>At4g12120.1 68417.m01924 cytokinesis-related Sec1 protein, putative
similar to cytokinesis-related Sec1 protein KEULE
[Arabidopsis thaliana] gi|12659318|gb|AAK01291; contains
Pfam domain, PF00995: Sec1 family
Length = 662
Score = 72.5 bits (170), Expect = 2e-13
Identities = 41/119 (34%), Positives = 64/119 (53%), Gaps = 13/119 (10%)
Frame = +1
Query: 280 LLHELTLQAMAYDLLPIENDVYKYDA-----SQGQLKEVLLDENDDLWVELRHQHIAVVS 444
L+HE T AM +DLL +E + Y ++ + + KEVLLDE D +WVELR HIA S
Sbjct: 268 LIHEWTYDAMCHDLLNMEGNKYTHEVPSKTGDKPEKKEVLLDEEDSIWVELRDAHIADAS 327
Query: 445 TAVTKNLXKFTESKRMGG--------GDKQSMRDLSQMIKKMPQYQKELSKYATHLRLA 597
+ + + F + GD S +DL +M+ +PQY +++ K + H+ +A
Sbjct: 328 ERLHEKMTNFVSKNKAAQLKHSSKDFGDLSS-KDLQKMVHALPQYSEQIDKLSLHVEIA 385
Score = 41.9 bits (94), Expect = 4e-04
Identities = 30/89 (33%), Positives = 46/89 (51%), Gaps = 9/89 (10%)
Frame = +3
Query: 39 QTRNANMERIAEQIATLCATLGEYPSVRYRS----DWERNVELAQLIQQKLDA-----YK 191
Q +A + +A++IAT+ A+L EYP VRYR D +LI KL A
Sbjct: 179 QRADACLNVVAKRIATVLASLKEYPFVRYRGAKALDATTMTTYRELIPTKLAASVWNCLA 238
Query: 192 ADEPTMGEGPEKARSQLLVLDRGFDCVSP 278
+ T+ + P+ +LL+LDR D ++P
Sbjct: 239 RYKQTIEDFPQTETCELLILDRSIDQIAP 267
>At5g46540.1 68418.m05730 ABC transporter family protein contains
Pfam profile: PF00005 ABC transporter; similar to
multidrug-resistant protein CjMDR1 GI:14715462 from
[Coptis japonica]
Length = 1248
Score = 29.5 bits (63), Expect = 2.1
Identities = 23/85 (27%), Positives = 33/85 (38%)
Frame = +3
Query: 6 DPKILCLLALAQTRNANMERIAEQIATLCATLGEYPSVRYRSDWERNVELAQLIQQKLDA 185
+PKIL L +A ERI + V +R R ++ ++QQ
Sbjct: 513 NPKILLLDEATSALDAESERIVQDALVKLMLSRTTVVVAHRLTTIRTADMIAVVQQGKVI 572
Query: 186 YKADEPTMGEGPEKARSQLLVLDRG 260
K M + PE SQL+ L G
Sbjct: 573 EKGTHDEMIKDPEGTYSQLVRLQEG 597
>At4g23330.1 68417.m03362 eukaryotic translation initiation
factor-related contains weak similarity to
Swiss-Prot:Q9LD55 eukaryotic translation initiation
factor 3 subunit 10 (eIF-3 theta) (Eukaryotic
translation initiation factor 3 large subunit) (eIF3a)
(p114) [Arabidopsis thaliana]
Length = 259
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +2
Query: 284 YTN*LFKPWLTIFCQLKMMFTSMMHPRGS*RKF-SWMKMMT-YGSNCGTSILLW 439
Y N + W+T F +K++ +M P+ S F S K ++ +GS+ + LW
Sbjct: 105 YINSCLRLWITYFSYIKILIIKLMMPKNSKLTFRSLSKSLSLFGSSSPARLNLW 158
>At4g28300.2 68417.m04053 hydroxyproline-rich glycoprotein family
protein
Length = 438
Score = 28.3 bits (60), Expect = 4.8
Identities = 26/102 (25%), Positives = 50/102 (49%)
Frame = +1
Query: 298 LQAMAYDLLPIENDVYKYDASQGQLKEVLLDENDDLWVELRHQHIAVVSTAVTKNLXKFT 477
L+ ++ L +E Y D + G+++ L ++D V+LR ++ K+L +
Sbjct: 42 LEGLSSRLSQLELYCYNLDKTIGEMRSELTHAHEDADVKLR---------SLDKHLQEVH 92
Query: 478 ESKRMGGGDKQSMRDLSQMIKKMPQYQKELSKYATHLRLAED 603
S ++ DKQ + D + + K+ QKE S ++H + ED
Sbjct: 93 RSVQILR-DKQELADTQKELAKLQLVQKE-SSSSSHSQHGED 132
>At4g28300.1 68417.m04052 hydroxyproline-rich glycoprotein family
protein
Length = 496
Score = 28.3 bits (60), Expect = 4.8
Identities = 26/102 (25%), Positives = 50/102 (49%)
Frame = +1
Query: 298 LQAMAYDLLPIENDVYKYDASQGQLKEVLLDENDDLWVELRHQHIAVVSTAVTKNLXKFT 477
L+ ++ L +E Y D + G+++ L ++D V+LR ++ K+L +
Sbjct: 100 LEGLSSRLSQLELYCYNLDKTIGEMRSELTHAHEDADVKLR---------SLDKHLQEVH 150
Query: 478 ESKRMGGGDKQSMRDLSQMIKKMPQYQKELSKYATHLRLAED 603
S ++ DKQ + D + + K+ QKE S ++H + ED
Sbjct: 151 RSVQILR-DKQELADTQKELAKLQLVQKE-SSSSSHSQHGED 190
>At1g30160.1 68414.m03687 expressed protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 311
Score = 27.9 bits (59), Expect = 6.3
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = -2
Query: 373 LTAPGMHHTCKHHFQLAEDRKPWLEELV 290
L APG ++ C ED+KP ELV
Sbjct: 138 LLAPGGNYLCSWDLNFKEDKKPKFHELV 165
>At4g33630.2 68417.m04778 expressed protein
Length = 684
Score = 27.5 bits (58), Expect = 8.3
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -1
Query: 443 ETTAICWCRNSTHKSSFSSKRTSFNCPWDAS 351
+ TA+C C +S+ +S SS + N WD++
Sbjct: 40 DPTALCRCSSSSGSNSSSSSSSDDNPRWDSA 70
>At4g33630.1 68417.m04777 expressed protein
Length = 684
Score = 27.5 bits (58), Expect = 8.3
Identities = 11/31 (35%), Positives = 19/31 (61%)
Frame = -1
Query: 443 ETTAICWCRNSTHKSSFSSKRTSFNCPWDAS 351
+ TA+C C +S+ +S SS + N WD++
Sbjct: 40 DPTALCRCSSSSGSNSSSSSSSDDNPRWDSA 70
>At3g03530.1 68416.m00353 phosphoesterase family protein low
similarity to SP|P95246 Phospholipase C 2 precursor (EC
3.1.4.3) {Mycobacterium tuberculosis}; contains Pfam
profile PF04185: Phosphoesterase family
Length = 538
Score = 27.5 bits (58), Expect = 8.3
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = -2
Query: 307 WLEELVRVVNGDTQSNPRSRTRS 239
W +EL R ++G T+S+P+S T S
Sbjct: 33 WFKELNREIDGVTKSDPKSNTVS 55
>At1g56690.1 68414.m06520 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 704
Score = 27.5 bits (58), Expect = 8.3
Identities = 12/48 (25%), Positives = 28/48 (58%)
Frame = +1
Query: 313 YDLLPIENDVYKYDASQGQLKEVLLDENDDLWVELRHQHIAVVSTAVT 456
YD++P+++ V + G +E +DE ++ E+R +++ +T +T
Sbjct: 164 YDMMPVKDVVASTNMIGGLCREGRVDEARLIFDEMRERNVVTWTTMIT 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,464,865
Number of Sequences: 28952
Number of extensions: 287500
Number of successful extensions: 822
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 800
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 816
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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