BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_A17 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 28 4.7 >At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, putative similar to dihydrolipoamide acetyltransferase (E2) subunit of PDC [Arabidopsis thaliana] GI:559395; contains Pfam profiles PF00198: 2-oxo acid dehydrogenases acyltransferase (catalytic domain), PF00364: Biotin-requiring enzyme, PF02817: e3 binding domain; supporting cDNA gi|5881964|gb|AF066080.1|AF066080 Length = 637 Score = 28.3 bits (60), Expect = 4.7 Identities = 26/106 (24%), Positives = 42/106 (39%), Gaps = 1/106 (0%) Frame = +3 Query: 159 PFKMADKTIPIFVPSEKPVKLAKWKIKQGTVVTQGQVLFLYKDLXGDGEXLKKFKSSHAG 338 P +P P+ + KW K+G V G VL ++ D + +F+S G Sbjct: 82 PISQTVLAMPALSPTMSHGNVVKWMKKEGDKVEVGDVLC---EIETD-KATVEFESQEEG 137 Query: 339 TVSSIXVKEGXVXXP-SSAVAXLEECRHPTVMMXMCAECGADLRSE 473 ++ I V EG P + +A + E + E G D + E Sbjct: 138 FLAKILVTEGSKDIPVNEPIAIMVEEEDDIKNVPATIEGGRDGKEE 183 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,989,449 Number of Sequences: 28952 Number of extensions: 255343 Number of successful extensions: 550 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 537 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 550 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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