BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P02_F_A03 (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schiz... 134 1e-32 SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Sch... 50 2e-07 SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyc... 33 0.048 SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe... 27 3.1 SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces po... 26 4.1 SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces po... 26 5.5 SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac... 26 5.5 SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr ... 25 7.2 SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|c... 25 9.5 SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|... 25 9.5 >SPAC22H10.12c |gdi1|sec19|GDP dissociation inhibitor Gdi1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 440 Score = 134 bits (323), Expect = 1e-32 Identities = 65/129 (50%), Positives = 89/129 (68%), Gaps = 1/129 (0%) Frame = +1 Query: 262 LEELFAKFN-APAPDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGVTRYLEFKSIEGS 438 L +L+A F E+ GR RDW VDL+PKFLMANG L +LI+T VTRY+EFK I GS Sbjct: 48 LTQLYALFRPGEQRPESLGRDRDWCVDLVPKFLMANGDLTNILIYTDVTRYIEFKQIAGS 107 Query: 439 YVYKGGKISKVPVDQKEALASDLMGMFXKRRFRNFLIYVQXFQXEDAXTWXDFDPSTANM 618 YVY+ G+I+KVP ++ EAL S LM +F KRR + FL +V ++ +D T+ D + +M Sbjct: 108 YVYRDGRIAKVPGNEMEALKSPLMSLFEKRRAKKFLEWVNNYREDDPSTYKDINIDRDSM 167 Query: 619 QSLYDKFGL 645 +S++ KFGL Sbjct: 168 ESVFKKFGL 176 Score = 85.8 bits (203), Expect = 5e-18 Identities = 38/46 (82%), Positives = 42/46 (91%) Frame = +3 Query: 117 MDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASI 254 MDEEYDVIVLGTGL EC+LSG+LSV GKKVLHIDRN YYG +SAS+ Sbjct: 1 MDEEYDVIVLGTGLTECVLSGLLSVDGKKVLHIDRNDYYGADSASL 46 >SPBC15C4.03 |||Rab geranylgeranyltransferase escort protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 459 Score = 50.4 bits (115), Expect = 2e-07 Identities = 22/43 (51%), Positives = 30/43 (69%) Frame = +3 Query: 129 YDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASIT 257 YDVI++GT L+ ILS LS + ++VLHID N +YG S+T Sbjct: 7 YDVIIVGTNLRNSILSAALSWANQRVLHIDENSFYGEIDGSLT 49 Score = 26.2 bits (55), Expect = 4.1 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%) Frame = +1 Query: 346 PKFLMANGLLVKLLIHTGVTRYLEFKSIEG-SYVYKGGKISKVPVDQKEALASDLMGMFX 522 P+ + A+ LVKLL T + +YL K + + KVP + + + + + Sbjct: 104 PQEIFASSELVKLLSETKIYKYLLLKPARSFRLLTSNEEWIKVPESRADIFNNKNLSLAS 163 Query: 523 KRRFRNFLIYVQXFQXE 573 KR F+ +V E Sbjct: 164 KRIVMRFMKFVSNIADE 180 >SPBC713.12 |erg1||squalene monooxygenase Erg1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 32.7 bits (71), Expect = 0.048 Identities = 12/34 (35%), Positives = 22/34 (64%) Frame = +3 Query: 123 EEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRN 224 ++ D+I++G G+ C L L G+KVL ++R+ Sbjct: 4 QDADIIIIGAGITGCALGAALGRQGRKVLVLERD 37 >SPBC2G2.11 |||N-myristoyltransferase 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/31 (38%), Positives = 15/31 (48%) Frame = +1 Query: 262 LEELFAKFNAPAPDETYGRGRDWNVDLIPKF 354 L L K A AP +TY + W +PKF Sbjct: 32 LRSLIEKEEAAAPPKTYEDFKFWKTQPVPKF 62 >SPAC17H9.03c |rdl1||RAD51D-like protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 230 Score = 26.2 bits (55), Expect = 4.1 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = +1 Query: 184 CRFLGRRFCTSIAISTTVVNLRQL 255 CR L R+F ++ +ST++V ++QL Sbjct: 116 CRILTRKFRLAVYLSTSLVYIKQL 139 >SPAPB17E12.08 |||N-glycosylation protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 166 Score = 25.8 bits (54), Expect = 5.5 Identities = 13/37 (35%), Positives = 20/37 (54%) Frame = +1 Query: 292 PAPDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGV 402 P P + G + + V F++ANG L+K LIH + Sbjct: 14 PRPAQALGINQPF-VSAYLSFVLANGFLLKWLIHYSI 49 >SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosaccharomyces pombe|chr 3|||Manual Length = 477 Score = 25.8 bits (54), Expect = 5.5 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 1/86 (1%) Frame = -3 Query: 519 KHAHKIRRKSFFLIDRYFGDFSTFVNITSFDGLKLQVPGDARVNKQFD*QSISHQKLRNQ 340 K +HK+ ++S FL D + DF+ F+ I D + Q + R +F S + +R++ Sbjct: 378 KFSHKMLKRSQFLNDHFDADFN-FIQI-DHDPITNQ---ETRKPVKF---SKPTKDIRSR 429 Query: 339 V-NVPVSTSTVCFIRCRCVELSEQLL 265 + VP ST IR ++L E+ L Sbjct: 430 LKEVPTSTDEEFIIRQELMDLLEKCL 455 >SPAC17A2.05 |||fumerate reductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.4 bits (53), Expect = 7.2 Identities = 13/36 (36%), Positives = 19/36 (52%) Frame = +3 Query: 138 IVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGES 245 IV+G GL + + G VL +D+N +GG S Sbjct: 42 IVIGGGLAGLSATNTILDLGGNVLLLDKNTAFGGNS 77 >SPBC13G1.05 |||DUF747 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 649 Score = 25.0 bits (52), Expect = 9.5 Identities = 11/27 (40%), Positives = 15/27 (55%) Frame = +2 Query: 383 CLFTRASPGTWSLSPSKEVMFTKVEKS 463 C+F R S TWS+ S M ++ KS Sbjct: 487 CVFIRTSMQTWSMFRSTHSMKQEIAKS 513 >SPAC1F7.05 |cdc22||ribonucleoside reductase large subunit Cdc22|Schizosaccharomyces pombe|chr 1|||Manual Length = 811 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = -1 Query: 128 FFIHYDSVKDQLDLTNYWLKKCQNVGTENGKMSAIDDVAXMN 3 F ++ DS + + N +C N+ TE + S+ D+VA N Sbjct: 404 FMLYKDSCNRKSNQKNVGTIRCSNLCTEIVEYSSPDEVAVCN 445 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,649,124 Number of Sequences: 5004 Number of extensions: 53915 Number of successful extensions: 158 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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