Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_A03
(653 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g44100.1 68415.m05484 Rab GDP dissociation inhibitor (GDI1) i... 123 1e-28
At3g59920.1 68416.m06687 Rab GDP dissociation inhibitor (GDI2) i... 120 8e-28
At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putativ... 106 1e-23
At3g06540.1 68416.m00758 GDP dissociation inhibitor family prote... 51 6e-07
At4g19380.1 68417.m02853 alcohol oxidase-related similar to long... 33 0.17
At1g79020.1 68414.m09214 transcription factor-related similar to... 29 2.7
At1g06820.1 68414.m00727 carotenoid isomerase, putative similar ... 29 3.6
At5g24155.1 68418.m02841 squalene monooxygenase, putative / squa... 28 4.7
At3g23410.1 68416.m02951 alcohol oxidase-related similar to long... 28 4.7
At4g36070.1 68417.m05135 calcium-dependent protein kinase family... 28 6.2
At1g19770.1 68414.m02471 purine permease-related low similarity ... 27 8.2
>At2g44100.1 68415.m05484 Rab GDP dissociation inhibitor (GDI1)
identical to GDP dissociation inhibitor [Arabidopsis
thaliana] GI:1655424
Length = 445
Score = 123 bits (296), Expect = 1e-28
Identities = 61/129 (47%), Positives = 80/129 (62%), Gaps = 1/129 (0%)
Frame = +1
Query: 262 LEELFAKFNAPAPDETY-GRGRDWNVDLIPKFLMANGLLVKLLIHTGVTRYLEFKSIEGS 438
L +L+ KF + G RD+NVD++PKF+MANG LV++LIHT VT+YL FK+++GS
Sbjct: 48 LNQLWKKFRGEEKAPAHLGSSRDYNVDMMPKFMMANGKLVRVLIHTDVTKYLSFKAVDGS 107
Query: 439 YVYKGGKISKVPVDQKEALASDLMGMFXKRRFRNFLIYVQXFQXEDAXTWXDFDPSTANM 618
YV+ GK+ KVP EAL S LMG+F KRR F YVQ + +D T D
Sbjct: 108 YVFVQGKVQKVPATPMEALKSPLMGIFEKRRAGKFFSYVQEYDEKDPKTHDGMDLRRVTT 167
Query: 619 QSLYDKFGL 645
+ L KFGL
Sbjct: 168 KDLIAKFGL 176
Score = 85.0 bits (201), Expect = 4e-17
Identities = 38/46 (82%), Positives = 42/46 (91%)
Frame = +3
Query: 117 MDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASI 254
MDEEY+VIVLGTGLKECILSG+LSV G KVLH+DRN YYGGES S+
Sbjct: 1 MDEEYEVIVLGTGLKECILSGLLSVDGLKVLHMDRNDYYGGESTSL 46
>At3g59920.1 68416.m06687 Rab GDP dissociation inhibitor (GDI2)
identical to Rab GDP dissociation inhibitor AtGDI2
[Arabidopsis thaliana] GI:2446981
Length = 444
Score = 120 bits (289), Expect = 8e-28
Identities = 61/130 (46%), Positives = 81/130 (62%), Gaps = 2/130 (1%)
Frame = +1
Query: 262 LEELFAKFNAP--APDETYGRGRDWNVDLIPKFLMANGLLVKLLIHTGVTRYLEFKSIEG 435
L +L+ KF AP E G RD+NVD++PKF+M NG LV+ LIHT VT+YL FK+++G
Sbjct: 48 LNQLWKKFRGEEKAP-EHLGASRDYNVDMMPKFMMGNGKLVRTLIHTDVTKYLSFKAVDG 106
Query: 436 SYVYKGGKISKVPVDQKEALASDLMGMFXKRRFRNFLIYVQXFQXEDAXTWXDFDPSTAN 615
SYV+ GK+ KVP EAL S LMG+F KRR F +VQ + +D T D +
Sbjct: 107 SYVFVKGKVQKVPATPMEALKSSLMGIFEKRRAGKFFSFVQEYDEKDPKTHDGMDLTRVT 166
Query: 616 MQSLYDKFGL 645
+ L K+GL
Sbjct: 167 TKELIAKYGL 176
Score = 85.0 bits (201), Expect = 4e-17
Identities = 38/46 (82%), Positives = 42/46 (91%)
Frame = +3
Query: 117 MDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASI 254
MDEEY+VIVLGTGLKECILSG+LSV G KVLH+DRN YYGGES S+
Sbjct: 1 MDEEYEVIVLGTGLKECILSGLLSVDGVKVLHMDRNDYYGGESTSL 46
>At5g09550.1 68418.m01106 Rab GDP dissociation inhibitor, putative
strong similarity to GDP dissociation inhibitor protein
OsGDI1 [Oryza sativa] GI:2384758; contains Pfam profile
PF00996: GDP dissociation inhibitor
Length = 365
Score = 106 bits (254), Expect = 1e-23
Identities = 51/96 (53%), Positives = 64/96 (66%)
Frame = +1
Query: 358 MANGLLVKLLIHTGVTRYLEFKSIEGSYVYKGGKISKVPVDQKEALASDLMGMFXKRRFR 537
MANGLLV+ LIHT VT+YL FK+++GS+VYK GKI KVP EAL S LMG+F KRR R
Sbjct: 1 MANGLLVQTLIHTDVTKYLNFKAVDGSFVYKKGKIYKVPATDVEALKSPLMGLFEKRRAR 60
Query: 538 NFLIYVQXFQXEDAXTWXDFDPSTANMQSLYDKFGL 645
F IYVQ + +D + D S + + K+GL
Sbjct: 61 KFFIYVQDYDEKDPKSHEGLDLSKVTAREIISKYGL 96
>At3g06540.1 68416.m00758 GDP dissociation inhibitor family protein
/ Rab GTPase activator family protein similar to
SP|P26374 Rab proteins geranylgeranyltransferase
component A 2 (Rab escort protein 2) {Homo sapiens};
contains Pfam profile PF00996: GDP dissociation
inhibitor
Length = 563
Score = 51.2 bits (117), Expect = 6e-07
Identities = 23/56 (41%), Positives = 35/56 (62%)
Frame = +3
Query: 90 IELIFYTIIMDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASIT 257
I++ Y + YD+IV+GTG+ E +L+ S SG VLH+D N +YG AS++
Sbjct: 2 IDIPPYPPLDPSNYDLIVVGTGVSESVLAAAASSSGSSVLHLDPNPFYGSHFASLS 57
Score = 40.3 bits (90), Expect = 0.001
Identities = 26/82 (31%), Positives = 43/82 (52%), Gaps = 3/82 (3%)
Frame = +1
Query: 322 RDWNVDLI-PKFLMANGLLVKLLIHTGVTRYLEFKSIEGSYV-YKGGKISKVPVDQKEAL 495
R +NVDL P+ + + L++ +G Y+EFKSI+ S+V G++ VP D + A+
Sbjct: 118 RRFNVDLAGPRVVFCADESINLMLKSGANNYVEFKSIDASFVGDSSGELRNVP-DSRAAI 176
Query: 496 ASD-LMGMFXKRRFRNFLIYVQ 558
D + + K + F VQ
Sbjct: 177 FKDKSLTLLEKNQLMKFFKLVQ 198
>At4g19380.1 68417.m02853 alcohol oxidase-related similar to long
chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]
Length = 726
Score = 33.1 bits (72), Expect = 0.17
Identities = 15/47 (31%), Positives = 27/47 (57%)
Frame = +3
Query: 114 IMDEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGESASI 254
+M + D +V+G+G + +G+L+ +G KVL I+ YY S+
Sbjct: 219 VMKIQCDAVVVGSGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSL 265
>At1g79020.1 68414.m09214 transcription factor-related similar to
enhancer of polycomb (GI:3757890) [Drosophila
melanogaster]; similar to enhancer of polycomb
(GI:11907923) [Homo sapiens]
Length = 453
Score = 29.1 bits (62), Expect = 2.7
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = -3
Query: 576 IFXLEXLNVD*EVAETPLXKHAHKIRRKSFFLIDRYFGDFS-TFVNITSF 430
+ + + VD EVA P K A +I F ++D Y D+S TF S+
Sbjct: 41 LLRIASVEVDNEVAPVPSKKPASEIPTPQFVIVDTYERDYSPTFGQPASY 90
>At1g06820.1 68414.m00727 carotenoid isomerase, putative similar to
carotenoid isomerase from Lycopersicon esculentum
[gi:19550437]; contains Pfam profile: PF02032 Phytoene
dehydrogenase related enzyme
Length = 595
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/39 (33%), Positives = 23/39 (58%)
Frame = +3
Query: 129 YDVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYYGGES 245
YD IV+G+G+ + + L+V +VL +++ GG S
Sbjct: 78 YDAIVIGSGIGGLVAATQLAVKEARVLVLEKYLIPGGSS 116
>At5g24155.1 68418.m02841 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2)
Length = 121
Score = 28.3 bits (60), Expect = 4.7
Identities = 13/35 (37%), Positives = 22/35 (62%)
Frame = +3
Query: 120 DEEYDVIVLGTGLKECILSGMLSVSGKKVLHIDRN 224
D DVI++G G+ L+ L+ G++VL I+R+
Sbjct: 45 DSAADVIIVGAGVGGSALAYSLAKDGRRVLAIERD 79
>At3g23410.1 68416.m02951 alcohol oxidase-related similar to long
chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]
Length = 746
Score = 28.3 bits (60), Expect = 4.7
Identities = 11/34 (32%), Positives = 23/34 (67%)
Frame = +3
Query: 132 DVIVLGTGLKECILSGMLSVSGKKVLHIDRNKYY 233
DV+V+G+G + + +L+ SG KV+ +++ Y+
Sbjct: 234 DVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYF 267
>At4g36070.1 68417.m05135 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 536
Score = 27.9 bits (59), Expect = 6.2
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = +1
Query: 361 ANGLLVKLLIHTGVTRYLEFKSIEGSYVYKGGKISKVPVD 480
A + KLL+ R +++ S+V +GG+ S+VP+D
Sbjct: 301 AKDFVKKLLVKEPRARLTAAQALSHSWVKEGGEASEVPID 340
>At1g19770.1 68414.m02471 purine permease-related low similarity to
purine permease [Arabidopsis thaliana] GI:7620007;
contains Pfam profile PF03151: Domain of unknown
function, DUF250
Length = 393
Score = 27.5 bits (58), Expect = 8.2
Identities = 17/46 (36%), Positives = 25/46 (54%)
Frame = +3
Query: 39 SVLSSDILTFLQPVVG*IELIFYTIIMDEEYDVIVLGTGLKECILS 176
SVLS+ I P+V + +IF+ MD+E+D G L +LS
Sbjct: 334 SVLSNVISVITWPIVSVLVVIFFN-FMDDEFDAF-KGVALVTAVLS 377
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,800,780
Number of Sequences: 28952
Number of extensions: 283793
Number of successful extensions: 762
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 762
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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