Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P02_F_A02
(654 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 279 2e-76
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 279 3e-76
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 279 3e-76
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 154 1e-38
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 151 6e-38
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 98 1e-21
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 50 4e-07
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 44 2e-05
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 39 5e-04
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 35 0.012
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 33 0.036
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 31 0.11
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 31 0.11
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 30 0.25
SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces p... 30 0.34
SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr 1||... 29 0.44
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.44
SPCC63.03 |||DNAJ domain protein, DNAJC11 family|Schizosaccharom... 27 1.8
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 27 2.4
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 279 bits (685), Expect = 2e-76
Identities = 132/168 (78%), Positives = 146/168 (86%)
Frame = +3
Query: 150 YKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYYVT 329
YKCGGIDKRTI KFEKEA E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT
Sbjct: 29 YKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVT 88
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGVKQL 509
+IDAPGHRDFIKNMITGTSQADCAVLI+ GTGEF GIS +GQTREHALLA+TLGVKQL
Sbjct: 89 VIDAPGHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQL 148
Query: 510 IVRVNKMDSTEPPYSEPXFXEIXXEVSSYIKKIGYNPXAVAFVPISGW 653
IV VNKMD+T +S+ F EI E S++IKK+G+NP V FVP+SG+
Sbjct: 149 IVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGF 194
Score = 59.3 bits (137), Expect = 5e-10
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLI 148
MGKEK HIN+VVIGHVDSGKSTTTGHLI
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLI 28
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 279 bits (684), Expect = 3e-76
Identities = 131/168 (77%), Positives = 146/168 (86%)
Frame = +3
Query: 150 YKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYYVT 329
YKCGGIDKRTI KFEKEA E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT
Sbjct: 29 YKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVT 88
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGVKQL 509
+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEF GIS +GQTREHALLA+TLGVKQL
Sbjct: 89 VIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQL 148
Query: 510 IVRVNKMDSTEPPYSEPXFXEIXXEVSSYIKKIGYNPXAVAFVPISGW 653
IV VNKMD+T +S+ F EI E S++IKK+G+NP V FVP+SG+
Sbjct: 149 IVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGF 194
Score = 59.3 bits (137), Expect = 5e-10
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLI 148
MGKEK HIN+VVIGHVDSGKSTTTGHLI
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLI 28
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 279 bits (684), Expect = 3e-76
Identities = 131/168 (77%), Positives = 146/168 (86%)
Frame = +3
Query: 150 YKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYYVT 329
YKCGGIDKRTI KFEKEA E+GKGSFKYAWVLDKLKAERERGITIDIALWKFET KY VT
Sbjct: 29 YKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVT 88
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGVKQL 509
+IDAPGHRDFIKNMITGTSQADCA+LI+ GTGEF GIS +GQTREHALLA+TLGVKQL
Sbjct: 89 VIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQL 148
Query: 510 IVRVNKMDSTEPPYSEPXFXEIXXEVSSYIKKIGYNPXAVAFVPISGW 653
IV VNKMD+T +S+ F EI E S++IKK+G+NP V FVP+SG+
Sbjct: 149 IVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGF 194
Score = 59.3 bits (137), Expect = 5e-10
Identities = 26/28 (92%), Positives = 27/28 (96%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLI 148
MGKEK HIN+VVIGHVDSGKSTTTGHLI
Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLI 28
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 154 bits (373), Expect = 1e-38
Identities = 80/167 (47%), Positives = 102/167 (61%), Gaps = 1/167 (0%)
Frame = +3
Query: 150 YKCGGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYYVT 329
++ G I+ R++ K EA GKGSF YAW+LD + ER RG+T+D+A FE+ K
Sbjct: 199 FELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYE 258
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGVKQL 509
I DAPGHRDFI MI G S AD AVL+V + F G NGQTREHA L LG+ ++
Sbjct: 259 IGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEI 318
Query: 510 IVRVNKMDSTEPPYSEPXFXEIXXEVSSY-IKKIGYNPXAVAFVPIS 647
+V VNK+D +SE F EI VS + IK +G+ V FVPIS
Sbjct: 319 VVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPIS 363
Score = 33.5 bits (73), Expect = 0.027
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = +2
Query: 77 KTHINIVVIGHVDSGKSTTTGHLILQM 157
K +++VV GHVDSGKST G ++ ++
Sbjct: 175 KPVVHLVVTGHVDSGKSTMLGRIMFEL 201
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 151 bits (367), Expect = 6e-38
Identities = 71/167 (42%), Positives = 106/167 (63%), Gaps = 2/167 (1%)
Frame = +3
Query: 159 GGIDKRTIXKFEKEAXEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYYVTIID 338
G +DKRT+ K E+EA E GK S+ +W LD ERE+G T+++ FET +++D
Sbjct: 263 GMVDKRTMEKIEREAKEAGKESWYLSWALDSTSEEREKGKTVEVGRAYFETEHRRFSLLD 322
Query: 339 APGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGVKQLIVR 518
APGH+ ++ NMI G SQAD VL+++A GEF G GQTREHA+LA T G+ L+V
Sbjct: 323 APGHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVV 382
Query: 519 VNKMDSTEPPYSEPXFXEIXXEVSSYIKKI-GYNPXA-VAFVPISGW 653
+NKMD +SE + E ++S +++++ GYN V ++P+S +
Sbjct: 383 INKMDEPSVQWSEERYKECVDKLSMFLRRVAGYNSKTDVKYMPVSAY 429
Score = 38.3 bits (85), Expect = 0.001
Identities = 15/24 (62%), Positives = 20/24 (83%)
Frame = +2
Query: 77 KTHINIVVIGHVDSGKSTTTGHLI 148
K H+NIV IGHVD+GKST G+++
Sbjct: 236 KEHVNIVFIGHVDAGKSTLGGNIL 259
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 97.9 bits (233), Expect = 1e-21
Identities = 53/130 (40%), Positives = 76/130 (58%)
Frame = +3
Query: 207 EMGKGSFKYAWVLDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTS 386
++G+ SF +DK E+ RGITI A ++ET + +D PGH D+IKNMITG +
Sbjct: 79 DLGQASFMDYSQIDKAPEEKARGITISSAHVEYETANRHYAHVDCPGHADYIKNMITGAA 138
Query: 387 QADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGVKQLIVRVNKMDSTEPPYSEPXF 566
D A+++V+A G+ QTREH LLA +GVKQ++V +NK+D EP E
Sbjct: 139 TMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVE 191
Query: 567 XEIXXEVSSY 596
E+ +S Y
Sbjct: 192 MEMRELLSEY 201
Score = 33.5 bits (73), Expect = 0.027
Identities = 13/22 (59%), Positives = 17/22 (77%)
Frame = +2
Query: 71 KEKTHINIVVIGHVDSGKSTTT 136
++K H+NI IGHVD GK+T T
Sbjct: 49 RKKPHVNIGTIGHVDHGKTTLT 70
>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 813
Score = 49.6 bits (113), Expect = 4e-07
Identities = 35/98 (35%), Positives = 47/98 (47%)
Frame = +3
Query: 240 VLDKLKAERERGITIDIALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAA 419
V+D L AER+RGITI+ A F + +ID PGH DF + + D AV I+
Sbjct: 67 VMDYLPAERQRGITINSAAISFTWRNQRINLIDTPGHADFTFEVERSVAVLDGAVAIIDG 126
Query: 420 GTGEFXXGISXNGQTREHALLAFTLGVKQLIVRVNKMD 533
G QT+ A G+ ++I VNKMD
Sbjct: 127 SAG-------VEAQTKVVWKQATKRGIPKVIF-VNKMD 156
Score = 25.4 bits (53), Expect = 7.2
Identities = 8/20 (40%), Positives = 15/20 (75%)
Frame = +2
Query: 89 NIVVIGHVDSGKSTTTGHLI 148
N+ +I H+D+GK+T T ++
Sbjct: 30 NVGIIAHIDAGKTTLTEKML 49
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 44.0 bits (99), Expect = 2e-05
Identities = 30/101 (29%), Positives = 50/101 (49%), Gaps = 4/101 (3%)
Frame = +3
Query: 243 LDKLKAERERGITIDIA----LWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 410
LDKL+ ER RGIT+ ++ + Y + +ID PGH DF ++ + + +L+
Sbjct: 95 LDKLEVERRRGITVKAQTCSMIYYYHGQSYLLNLIDTPGHVDFRAEVMHSLAACEGCILL 154
Query: 411 VAAGTGEFXXGISXNGQTREHALLAFTLGVKQLIVRVNKMD 533
V A G QT + +AF+ + +I +NK+D
Sbjct: 155 VDASQG-------IQAQTLSNFYMAFSQNL-VIIPVLNKVD 187
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 39.1 bits (87), Expect = 5e-04
Identities = 22/85 (25%), Positives = 38/85 (44%)
Frame = +3
Query: 321 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREHALLAFTLGV 500
+V+ +D PGH + M+ G + D A+L++A QT EH + +
Sbjct: 109 HVSFVDCPGHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQL 162
Query: 501 KQLIVRVNKMDSTEPPYSEPXFXEI 575
K +I+ NK+D +E + I
Sbjct: 163 KHIIILQNKVDLIRESAAEEHYQSI 187
Score = 25.0 bits (52), Expect = 9.5
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +2
Query: 59 PKMGKEKTHINIVVIGHVDSGKST 130
P + + INI IGHV GKST
Sbjct: 15 PAIISRQATINIGTIGHVAHGKST 38
>SPBC1271.15c |||translation initiation factor
IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
Length = 686
Score = 34.7 bits (76), Expect = 0.012
Identities = 22/74 (29%), Positives = 33/74 (44%), Gaps = 2/74 (2%)
Frame = +3
Query: 213 GKGSFKYAWVLDKLKAERERGITIDIALWK--FETXKYYVTIIDAPGHRDFIKNMITGTS 386
GK + A+ + + GIT I + F+ ++T +D PGH F G +
Sbjct: 183 GKTTLLDAFRKSTIASTEHGGITQKIGAFTVPFDKGSKFITFLDTPGHMAFEAMRKRGAN 242
Query: 387 QADCAVLIVAAGTG 428
AD VL+VA G
Sbjct: 243 IADIVVLVVAGDDG 256
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 33.1 bits (72), Expect = 0.036
Identities = 18/52 (34%), Positives = 26/52 (50%)
Frame = +3
Query: 318 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFXXGISXNGQTREH 473
Y + IID PGH DF + D AVL++ A +G I+ + Q R +
Sbjct: 145 YNINIIDTPGHIDFTIEVERALRVLDGAVLVLCAVSGVQSQTITVDRQMRRY 196
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 31.5 bits (68), Expect = 0.11
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLI 148
MGK N+ VI HVD GKST T L+
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLV 40
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 318 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 413
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 31.5 bits (68), Expect = 0.11
Identities = 15/28 (53%), Positives = 17/28 (60%)
Frame = +2
Query: 65 MGKEKTHINIVVIGHVDSGKSTTTGHLI 148
MGK N+ VI HVD GKST T L+
Sbjct: 13 MGKPSNVRNMSVIAHVDHGKSTLTDSLV 40
Score = 27.9 bits (59), Expect = 1.4
Identities = 11/32 (34%), Positives = 18/32 (56%)
Frame = +3
Query: 318 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 413
+ V +ID+PGH DF + D A+++V
Sbjct: 98 FLVNLIDSPGHVDFSSEVTAALRVTDGALVVV 129
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1000
Score = 30.3 bits (65), Expect = 0.25
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 318 YYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 428
Y + +ID+PGH DF + + + D A ++V A G
Sbjct: 94 YLINLIDSPGHVDFSSEVSSASRLCDGAFVLVDAVEG 130
>SPAPYUG7.03c |mid2||anillin homologue Mid2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 29.9 bits (64), Expect = 0.34
Identities = 14/43 (32%), Positives = 23/43 (53%)
Frame = -3
Query: 283 IVIPRSRSAFSLSNTQAYLKDPLPISWASFSNXSMVRLSIPPH 155
I IP+ A S+ N++ + +D LP + S V+L +P H
Sbjct: 171 IPIPKKNPARSVCNSKLFNEDTLPAEFEEVSISPPVKLELPTH 213
>SPAC9G1.11c |spn4||septin Spn4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 380
Score = 29.5 bits (63), Expect = 0.44
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 3/44 (6%)
Frame = +3
Query: 246 DKLKAER-ERGITIDIALWKFETXKYYV--TIIDAPGHRDFIKN 368
+K++A+ E+ + I+I + E +++ T+ID PG DFI N
Sbjct: 60 EKVRAKHAEKTVEIEITKAELEEKNFHLRLTVIDTPGFGDFINN 103
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.5 bits (63), Expect = 0.44
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 330 IIDAPGHRDFIKNMITGTSQADCAVLIV 413
IID PGH F GTS + A+L++
Sbjct: 553 IIDTPGHESFTNLRSRGTSLCNIAILVI 580
>SPCC63.03 |||DNAJ domain protein, DNAJC11
family|Schizosaccharomyces pombe|chr 3|||Manual
Length = 642
Score = 27.5 bits (58), Expect = 1.8
Identities = 16/44 (36%), Positives = 22/44 (50%)
Frame = -3
Query: 307 SNFQRAISIVIPRSRSAFSLSNTQAYLKDPLPISWASFSNXSMV 176
S F IS+ +P FSL+ ++ K LPI W S + S V
Sbjct: 403 STFGVNISVGVPTGSITFSLNWSRLGQKISLPIMWCSVFDRSAV 446
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 27.1 bits (57), Expect = 2.4
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +3
Query: 261 ERERGITID-----IALWKFETXKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 413
ERER ++I +A+ + + ID PGH DF+ + + +D VL+V
Sbjct: 185 ERERVMSIKSTPLTLAVSDMKGKTFAFQCIDTPGHVDFVDEVAAPMAISDGVVLVV 240
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,366,755
Number of Sequences: 5004
Number of extensions: 41356
Number of successful extensions: 143
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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