Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_P19
(582 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53280.1 68414.m06038 DJ-1 family protein similar to DJ-1 pro... 90 9e-19
At3g14990.2 68416.m01896 4-methyl-5(b-hydroxyethyl)-thiazole mon... 77 7e-15
At3g14990.1 68416.m01895 4-methyl-5(b-hydroxyethyl)-thiazole mon... 77 7e-15
At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1 [Ra... 75 5e-14
At3g02720.1 68416.m00263 DJ-1 family protein / protease-related ... 34 0.060
At1g49600.1 68414.m05561 RNA-binding protein 47 (RBP47), putativ... 27 6.9
>At1g53280.1 68414.m06038 DJ-1 family protein similar to DJ-1
protein [Homo sapiens] GI:1780755; similar to DJ-1 beta
(GI:18642508) [Drosophila melanogaster]; contains Pfam
profile: PF01965 ThiJ/PfpI family; TIGRFAM TIGR01383:
DJ-1 family protein
Length = 438
Score = 90.2 bits (214), Expect = 9e-19
Identities = 54/156 (34%), Positives = 83/156 (53%), Gaps = 1/156 (0%)
Frame = +2
Query: 107 EMETVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPX 286
EME V +D+L+R V +A L S V+ SR+V LV D L EA EK YD ++LP
Sbjct: 270 EMEAVAIIDVLKRAKANVVVAALGNSLEVVASRKVKLVADVLLDEA--EKNSYDLIVLPG 327
Query: 287 XXXXXXXXXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXX-HGVARGRRVTSYPSTRDKL 463
+ +LK ++ K HG+ +G++ T++P+ KL
Sbjct: 328 GLGGAEAFASSEKLVNMLKKQAESNKPYGAICASPALVFEPHGLLKGKKATAFPAMCSKL 387
Query: 464 SAGDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTLI 571
+ D +++E RV+VDGN++TSRGPGT+ F L ++
Sbjct: 388 T--DQSHIE-HRVLVDGNLITSRGPGTSLEFALAIV 420
Score = 70.5 bits (165), Expect = 7e-13
Identities = 48/157 (30%), Positives = 74/157 (47%), Gaps = 1/157 (0%)
Frame = +2
Query: 113 ETVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPXXX 292
E V+ +D+LRRGG VT+A +E V + +V D L++ +D ++LP
Sbjct: 67 EAVVMIDVLRRGGADVTVASVENQVGVDACHGIKMVADTLLSDIT--DSVFDLIMLPGGL 124
Query: 293 XXXXXXXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXXH-GVARGRRVTSYPSTRDKLSA 469
+ ++K +G+ G+ G++ T YP +KL+A
Sbjct: 125 PGGETLKNCKPLEKMVKKQDTDGRLNAAICCAPALAFGTWGLLEGKKATCYPVFMEKLAA 184
Query: 470 GDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTLIXLL 580
T VE RV +DG +VTSRGPGT F +TL+ L
Sbjct: 185 CA-TAVES-RVEIDGKIVTSRGPGTTMEFSVTLVEQL 219
>At3g14990.2 68416.m01896 4-methyl-5(b-hydroxyethyl)-thiazole
monophosphate biosynthesis protein, putative supporting
cDNA gi|11908017|gb|AF326856.1|; contains Pfam profile
PF01965:DJ-1/PfpI family; identical to cDNA EST Athsr7
GI:5281061
Length = 369
Score = 77.4 bits (182), Expect = 7e-15
Identities = 54/156 (34%), Positives = 78/156 (50%), Gaps = 1/156 (0%)
Frame = +2
Query: 107 EMETVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPX 286
E+E + VD+LRR V +A + S V SR+ LV + L E +AEK +D ++LP
Sbjct: 201 EIEAIALVDILRRAKANVVIAAVGNSLEVEGSRKAKLVAEVLLDE-VAEKS-FDLIVLPG 258
Query: 287 XXXXXXXXXXXXX-VGALLKDHXDNGKXXXXXXXXXXXXXXHGVARGRRVTSYPSTRDKL 463
V L K N +G+ +G++ T++P DKL
Sbjct: 259 GLNGAQRFASCEKLVNMLRKQAEANKPYGGICASPAYVFEPNGLLKGKKATTHPVVSDKL 318
Query: 464 SAGDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTLI 571
S D +++E RVVVDGNV+TSR PGTA F L ++
Sbjct: 319 S--DKSHIE-HRVVVDGNVITSRAPGTAMEFSLAIV 351
Score = 68.1 bits (159), Expect = 4e-12
Identities = 46/152 (30%), Positives = 70/152 (46%), Gaps = 1/152 (0%)
Frame = +2
Query: 128 VDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPXXXXXXXX 307
+ +LRRGG VT+A +E V + +V D L++ +D ++LP
Sbjct: 2 ITVLRRGGADVTVASVETQVGVDACHGIKMVADTLLSDIT--DSVFDLIVLPGGLPGGET 59
Query: 308 XXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXXH-GVARGRRVTSYPSTRDKLSAGDYTY 484
+ ++K +G+ G+ G++ T YP +KL+A T
Sbjct: 60 LKNCKSLENMVKKQDSDGRLNAAICCAPALALGTWGLLEGKKATGYPVFMEKLAATCATA 119
Query: 485 VEGERVVVDGNVVTSRGPGTAYWFGLTLIXLL 580
VE RV +DG +VTSRGPGT F +TLI L
Sbjct: 120 VES-RVQIDGRIVTSRGPGTTIEFSITLIEQL 150
>At3g14990.1 68416.m01895 4-methyl-5(b-hydroxyethyl)-thiazole
monophosphate biosynthesis protein, putative supporting
cDNA gi|11908017|gb|AF326856.1|; contains Pfam profile
PF01965:DJ-1/PfpI family; identical to cDNA EST Athsr7
GI:5281061
Length = 392
Score = 77.4 bits (182), Expect = 7e-15
Identities = 54/156 (34%), Positives = 78/156 (50%), Gaps = 1/156 (0%)
Frame = +2
Query: 107 EMETVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPX 286
E+E + VD+LRR V +A + S V SR+ LV + L E +AEK +D ++LP
Sbjct: 224 EIEAIALVDILRRAKANVVIAAVGNSLEVEGSRKAKLVAEVLLDE-VAEKS-FDLIVLPG 281
Query: 287 XXXXXXXXXXXXX-VGALLKDHXDNGKXXXXXXXXXXXXXXHGVARGRRVTSYPSTRDKL 463
V L K N +G+ +G++ T++P DKL
Sbjct: 282 GLNGAQRFASCEKLVNMLRKQAEANKPYGGICASPAYVFEPNGLLKGKKATTHPVVSDKL 341
Query: 464 SAGDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTLI 571
S D +++E RVVVDGNV+TSR PGTA F L ++
Sbjct: 342 S--DKSHIE-HRVVVDGNVITSRAPGTAMEFSLAIV 374
Score = 71.7 bits (168), Expect = 3e-13
Identities = 48/158 (30%), Positives = 73/158 (46%), Gaps = 1/158 (0%)
Frame = +2
Query: 110 METVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPXX 289
+E V + +LRRGG VT+A +E V + +V D L++ +D ++LP
Sbjct: 19 LEAVAMITVLRRGGADVTVASVETQVGVDACHGIKMVADTLLSDIT--DSVFDLIVLPGG 76
Query: 290 XXXXXXXXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXXH-GVARGRRVTSYPSTRDKLS 466
+ ++K +G+ G+ G++ T YP +KL+
Sbjct: 77 LPGGETLKNCKSLENMVKKQDSDGRLNAAICCAPALALGTWGLLEGKKATGYPVFMEKLA 136
Query: 467 AGDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTLIXLL 580
A T VE RV +DG +VTSRGPGT F +TLI L
Sbjct: 137 ATCATAVES-RVQIDGRIVTSRGPGTTIEFSITLIEQL 173
>At4g34020.1 68417.m04827 DJ-1 family protein similar to CAP1
[Rattus norvegicus] GI:3250916, ThiJ protein,
Escherichia coli, PIR:H64771; contains Pfam profile
PF01965 ThiJ/PfpI family
Length = 472
Score = 74.5 bits (175), Expect = 5e-14
Identities = 46/157 (29%), Positives = 74/157 (47%)
Frame = +2
Query: 110 METVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPXX 289
+E V D+LRR V VT++ +E S + + ++ DK + EA + YD +ILP
Sbjct: 300 VELVSIADVLRRAKVDVTVSSVERSLRITAFQGTKIITDKLIGEAA--ESSYDLIILPGG 357
Query: 290 XXXXXXXXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXXHGVARGRRVTSYPSTRDKLSA 469
+ LL++ ++G+ HG+ + +R T YPS D+
Sbjct: 358 HTGSERLQKSKILKKLLREQHESGRIYGATNSSSTVLHKHGLLKEKRTTVYPSESDEPM- 416
Query: 470 GDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTLIXLL 580
+ +EG VV+DGNV+TS G T F L ++ L
Sbjct: 417 -NQQMIEGAEVVIDGNVITSLGLATVTKFSLAIVSKL 452
Score = 53.6 bits (123), Expect = 9e-08
Identities = 41/155 (26%), Positives = 67/155 (43%), Gaps = 1/155 (0%)
Frame = +2
Query: 107 EMETVITVDMLRRGGVTVTLAGLEGSSPVLCSRQVTLVPDKSLTEALAEKQQYDAVILPX 286
E+E V+ VD+LRR G VT+A +E V S L+ D +++ Q YD V LP
Sbjct: 96 EIEAVVLVDVLRRAGADVTVASVEQKLEVEGSSGTRLLADVLISKCA--DQVYDLVALPG 153
Query: 287 XXXXXXXXXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXXH-GVARGRRVTSYPSTRDKL 463
+ ++K ++ + G+ +R T +P+ KL
Sbjct: 154 GMPGAVRLRDCEILEKIMKRQAEDKRLYGAISMAPAITLLPWGLLTRKRTTGHPAFFGKL 213
Query: 464 SAGDYTYVEGERVVVDGNVVTSRGPGTAYWFGLTL 568
+ + + G + TSRGPGT++ F L+L
Sbjct: 214 PT---FWAVKTNIQISGELTTSRGPGTSFQFALSL 245
>At3g02720.1 68416.m00263 DJ-1 family protein / protease-related
similar to Intracellular Protease [Pyrococcus
horikoshii] GI:11513902; contains Pfam profile: PF01965
ThiJ/PfpI family
Length = 388
Score = 34.3 bits (75), Expect = 0.060
Identities = 24/97 (24%), Positives = 43/97 (44%), Gaps = 4/97 (4%)
Frame = +2
Query: 263 YDAVILPXXXXXXXXXXXXXXVGALLKDHXDNGKXXXXXXXXXXXXXXHGVARGRRVTSY 442
YDA+++P + ++K+ ++ K GV +GR+ T+Y
Sbjct: 275 YDALVIPGGRAPEYLALNEHVLN-IVKEFMNSEKPVASICHGQQILAAAGVLKGRKCTAY 333
Query: 443 PSTRDKLSAGDYTYVEG---ERVVVDGNVVTSRG-PG 541
P+ + + G T++E +R DGN+VT PG
Sbjct: 334 PAVKLNVVLGGGTWLEPDPIDRCFTDGNLVTGAAWPG 370
>At1g49600.1 68414.m05561 RNA-binding protein 47 (RBP47), putative
similar to DNA binding protein ACBF GB:U90212 GI:1899187
from [Nicotiana tabacum]
Length = 445
Score = 27.5 bits (58), Expect = 6.9
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2
Query: 467 AGDYTYVEGERVVVDGNVVTSRGPG 541
AG Y V+G +VV+D N S+G G
Sbjct: 234 AGRYPSVKGAKVVIDSNTGRSKGYG 258
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,020,366
Number of Sequences: 28952
Number of extensions: 170340
Number of successful extensions: 410
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 394
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 405
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1141585696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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