Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_P02
(603 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 30 1.4
At4g20820.1 68417.m03021 FAD-binding domain-containing protein s... 29 2.4
At1g23230.1 68414.m02906 expressed protein 29 3.1
At4g22290.1 68417.m03224 ubiquitin carboxyl-terminal hydrolase f... 28 4.1
At5g22640.1 68418.m02645 MORN (Membrane Occupation and Recogniti... 28 5.5
At3g60720.1 68416.m06793 receptor-like protein kinase-related co... 28 5.5
At3g49590.2 68416.m05420 expressed protein 28 5.5
At3g49590.1 68416.m05419 expressed protein 28 5.5
At1g19000.2 68414.m02364 myb family transcription factor similar... 28 5.5
At1g19000.1 68414.m02363 myb family transcription factor similar... 28 5.5
At2g20950.1 68415.m02471 expressed protein 27 7.2
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 27 7.2
At5g16400.1 68418.m01917 thioredoxin, putative similar to SP|P29... 27 9.6
At5g16120.1 68418.m01883 hydrolase, alpha/beta fold family prote... 27 9.6
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 27 9.6
At1g70620.1 68414.m08138 cyclin-related contains weak similarity... 27 9.6
At1g49010.1 68414.m05495 myb family transcription factor contain... 27 9.6
>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 protein,
Xenopus laevis, PIR:T30335
Length = 1229
Score = 29.9 bits (64), Expect = 1.4
Identities = 24/96 (25%), Positives = 41/96 (42%)
Frame = +1
Query: 112 ESARRLQHQLSPXTSTPTEKIVLPSAEDVATXKTQKSLFDGIEKFDSSQLKHTETQEKNP 291
E+A + + +L S+P +K + + D S E +S++LK QEK
Sbjct: 834 ENAMKEKDELKRLLSSPDQKKPIEANSDTEMELCNISSEKSTEDLNSAKLKLELAQEKLS 893
Query: 292 LPDKDVVAAEKAHQNLLDGVEHFDKTQMKHTTTEEK 399
+ K + +N+LD + K + TEEK
Sbjct: 894 ISAKTIGVFSSLEENILDII----KLSKESKETEEK 925
>At4g20820.1 68417.m03021 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 532
Score = 29.1 bits (62), Expect = 2.4
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = -3
Query: 223 KTSGSXQWRRLQQTEAQSFQLVST 152
KT+ S WRRLQ+ EA+ QL+ T
Sbjct: 381 KTAISKLWRRLQEPEAEHAQLIFT 404
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 28.7 bits (61), Expect = 3.1
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = -3
Query: 532 PLWGAGSSRTFPCASA*WDRSSRC-RSGICSFPSP 431
PL+G + + P S W+R+ RC R I + PSP
Sbjct: 393 PLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSP 427
>At4g22290.1 68417.m03224 ubiquitin carboxyl-terminal hydrolase
family protein similar to pVHL-interacting
deubiquitinating enzyme 1 type II [Homo sapiens]
GI:18698435; contains Pfam profile PF00443: Ubiquitin
carboxyl-terminal hydrolase
Length = 974
Score = 28.3 bits (60), Expect = 4.1
Identities = 24/109 (22%), Positives = 45/109 (41%), Gaps = 4/109 (3%)
Frame = +3
Query: 282 EEPASGQRCCRSGESPPEPLGRS*TLRQDSDEAHDDGRKESTAPDRSYRSGEGKEQIPER 461
E +R +SPP PLG + L ++ DD +E +S G G +
Sbjct: 446 EREVKRKRVMERSDSPPPPLGFNNPLLPLANTYDDDDEEEENEQKKSQARGNGVAKGEGN 505
Query: 462 HRELRSH-*AEAHGNVREEPAPHKGRH*AREISLNH---YFITVTRKCI 596
+++ E + ++ + KG+H +R + + Y TV R+ +
Sbjct: 506 GNKVKGEAQEEVDDDEDDDVSKGKGKH-SRHVEVRRDCPYLDTVNRQVL 553
>At5g22640.1 68418.m02645 MORN (Membrane Occupation and Recognition
Nexus) repeat-containing protein contains Pfam profile
PF02493: MORN repeat
Length = 871
Score = 27.9 bits (59), Expect = 5.5
Identities = 18/64 (28%), Positives = 32/64 (50%)
Frame = +1
Query: 208 KTQKSLFDGIEKFDSSQLKHTETQEKNPLPDKDVVAAEKAHQNLLDGVEHFDKTQMKHTT 387
K+ K + DG+EK+ + K E +++ + ++V AE L + +E D+ K
Sbjct: 582 KSVKPMLDGLEKWTEEKKKAYEERKEMIQQELELVEAEIC---LEEAIEDMDEELKKKEQ 638
Query: 388 TEEK 399
EEK
Sbjct: 639 EEEK 642
>At3g60720.1 68416.m06793 receptor-like protein kinase-related
contains Pfam PF01657: Domain of unknown function,
duplicated in 33kDa secretory proteins; weak similarity
to receptor-like protein kinase homolog RK20-1
(GI:4530126) [Phaseolus vulgaris]
Length = 279
Score = 27.9 bits (59), Expect = 5.5
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = -2
Query: 245 NFSIPSNKDFWVXSVATSSADGS-TIFSVGVDVXGDS 138
N SN+D ++ SV TSS+D S F+VG D S
Sbjct: 42 NTPFESNRDTFLSSVVTSSSDASFNSFAVGNDSSSSS 78
>At3g49590.2 68416.m05420 expressed protein
Length = 603
Score = 27.9 bits (59), Expect = 5.5
Identities = 30/111 (27%), Positives = 41/111 (36%), Gaps = 4/111 (3%)
Frame = +3
Query: 75 PERPPQGSPQT*RVSSKASTPAVSXDVDTN*KDCASVC*RRRH*XDPEVFIRRYREV*FE 254
P P GSP T R S ++P ++ T S R+ R F
Sbjct: 338 PPFSPSGSPSTPRYISGGNSPRINVRPGTAPVTIPSSATLNRYVSSNFSEPGRNPLPPFS 397
Query: 255 PAEAHRD-SGEEPASG---QRCCRSGESPPEPLGRS*TLRQDSDEAHDDGR 395
P R S ++ G R RSGESP + + T + D +D GR
Sbjct: 398 PKSTRRSPSSQDSLPGIALYRSSRSGESPSGLMNQYPTQKLSKDSKYDSGR 448
>At3g49590.1 68416.m05419 expressed protein
Length = 603
Score = 27.9 bits (59), Expect = 5.5
Identities = 30/111 (27%), Positives = 41/111 (36%), Gaps = 4/111 (3%)
Frame = +3
Query: 75 PERPPQGSPQT*RVSSKASTPAVSXDVDTN*KDCASVC*RRRH*XDPEVFIRRYREV*FE 254
P P GSP T R S ++P ++ T S R+ R F
Sbjct: 338 PPFSPSGSPSTPRYISGGNSPRINVRPGTAPVTIPSSATLNRYVSSNFSEPGRNPLPPFS 397
Query: 255 PAEAHRD-SGEEPASG---QRCCRSGESPPEPLGRS*TLRQDSDEAHDDGR 395
P R S ++ G R RSGESP + + T + D +D GR
Sbjct: 398 PKSTRRSPSSQDSLPGIALYRSSRSGESPSGLMNQYPTQKLSKDSKYDSGR 448
>At1g19000.2 68414.m02364 myb family transcription factor similar to
MybSt1 GI:7705206 from [Solanum tuberosum]
Length = 285
Score = 27.9 bits (59), Expect = 5.5
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +1
Query: 208 KTQKSLFD-GIEKFDSSQLKHTETQEKNPLPDKDVVAAEKAHQNLLD 345
+ + SLFD E ++ TQE +PLP+ ++ + ++A Q D
Sbjct: 158 RRRSSLFDITTETVTEMAMEQDPTQENSPLPETNISSGQQAMQVFTD 204
>At1g19000.1 68414.m02363 myb family transcription factor similar to
MybSt1 GI:7705206 from [Solanum tuberosum]
Length = 285
Score = 27.9 bits (59), Expect = 5.5
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +1
Query: 208 KTQKSLFD-GIEKFDSSQLKHTETQEKNPLPDKDVVAAEKAHQNLLD 345
+ + SLFD E ++ TQE +PLP+ ++ + ++A Q D
Sbjct: 158 RRRSSLFDITTETVTEMAMEQDPTQENSPLPETNISSGQQAMQVFTD 204
>At2g20950.1 68415.m02471 expressed protein
Length = 520
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +3
Query: 411 PDRSYRSGEGKEQIPERHRELRSH*AEAHGNVREEPAPHKGRH*AREISLNHY 569
PDR RS E +++ ER E ++ + H PAP+ R+ S Y
Sbjct: 120 PDRRKRSSEITKELYERMYEADANVSPFHPFRSRSPAPYNTHERGRDYSRERY 172
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 27.5 bits (58), Expect = 7.2
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +2
Query: 191 KTSPLXRPRSLYSTVSRS 244
++ PL R RSLYS+VSRS
Sbjct: 229 RSRPLSRSRSLYSSVSRS 246
>At5g16400.1 68418.m01917 thioredoxin, putative similar to SP|P29450
Thioredoxin F-type, chloroplast precursor (TRX-F) {Pisum
sativum}; contains Pfam profile: PF00085 Thioredoxin
Length = 185
Score = 27.1 bits (57), Expect = 9.6
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = -3
Query: 337 GSGGLSPLRQHL-CPEAGSSPESRCASAGSNQTSRYRRI 224
G+GG SP++QH P +G + + S G RRI
Sbjct: 23 GAGGFSPVKQHCRIPNSGVATKIGFCSGGGGVLDSGRRI 61
>At5g16120.1 68418.m01883 hydrolase, alpha/beta fold family protein
similar to monoglyceride lipase from [Homo sapiens]
GI:14594904, [Mus musculus] GI:2632162; contains Pfam
profile PF00561: hydrolase, alpha/beta fold family
Length = 351
Score = 27.1 bits (57), Expect = 9.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = +1
Query: 271 ETQEKNPLPDKDVVAAEKAHQNLLDG 348
E EK PDK +V E A+ +LL+G
Sbjct: 294 ELYEKAKSPDKKIVLYENAYHSLLEG 319
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 27.1 bits (57), Expect = 9.6
Identities = 18/58 (31%), Positives = 25/58 (43%)
Frame = +3
Query: 384 DDGRKESTAPDRSYRSGEGKEQIPERHRELRSH*AEAHGNVREEPAPHKGRH*AREIS 557
D+G +S DR KE+ R E + E H REE K +H +E+S
Sbjct: 778 DNGSSDSDVDDRKEAKRRRKEEKKTRKEEKKRRREERH-RKREERRGGKEKHKKQELS 834
>At1g70620.1 68414.m08138 cyclin-related contains weak similarity to
Swiss-Prot:P35662 cylicin I (Multiple-band polypeptide
I) [Bos taurus]
Length = 897
Score = 27.1 bits (57), Expect = 9.6
Identities = 18/69 (26%), Positives = 30/69 (43%)
Frame = +3
Query: 273 DSGEEPASGQRCCRSGESPPEPLGRS*TLRQDSDEAHDDGRKESTAPDRSYRSGEGKEQI 452
+S ++ R R SP R SDE+ DD +++S++ ++ GK +
Sbjct: 794 ESSDDSKRKSRSRRRSVSPSPVRSRRKRSSPSSDESSDDSKRKSSSKRKNRSPSPGKSR- 852
Query: 453 PERHRELRS 479
RH RS
Sbjct: 853 -RRHVSSRS 860
>At1g49010.1 68414.m05495 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 314
Score = 27.1 bits (57), Expect = 9.6
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = -1
Query: 456 QEFVLFLLRFDSFDRGQWILFFRRRVLHLSLVEVFNSVQEVLV 328
+E LFLL D F +G W R V+ + +V + Q+ +
Sbjct: 139 EEHRLFLLGLDKFGKGDWRSISRNFVISRTPTQVASHAQKYFI 181
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.312 0.128 0.362
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,544,588
Number of Sequences: 28952
Number of extensions: 226697
Number of successful extensions: 572
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 547
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 569
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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