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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fe100P01_F_O18
         (653 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15)                99   9e-36
SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35)            24   2.4  
SB_14043| Best HMM Match : Extensin_1 (HMM E-Value=0.75)               29   3.3  
SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   3.3  
SB_605| Best HMM Match : Calx-beta (HMM E-Value=0)                     29   3.3  
SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.)                29   3.3  
SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.4  
SB_1052| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   4.4  
SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13)                   28   5.8  
SB_43823| Best HMM Match : MAM (HMM E-Value=0)                         28   5.8  
SB_49607| Best HMM Match : No HMM Matches (HMM E-Value=.)              25   7.1  
SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.)               28   7.6  

>SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15)
          Length = 710

 Score = 98.7 bits (235), Expect(2) = 9e-36
 Identities = 43/60 (71%), Positives = 51/60 (85%)
 Frame = +2

Query: 119 TTMGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKV 298
           T   ++ DT F +G+SGAS T+P QCS+LRKNG V++KGRPCKIVEMSTSKTGKHGHAKV
Sbjct: 585 TMAEELADTEFHSGESGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKV 644



 Score = 69.7 bits (163), Expect(2) = 9e-36
 Identities = 29/62 (46%), Positives = 43/62 (69%)
 Frame = +2

Query: 398 QLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGXELLCTVLKSCGEECVIA 577
           ++T+I +DGYL LM DNGD R D+K+ D D+  ++R  F++    + TVLK+ GEE V+ 
Sbjct: 643 KVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVG 702

Query: 578 VK 583
           VK
Sbjct: 703 VK 704


>SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35)
          Length = 458

 Score = 24.2 bits (50), Expect(2) = 2.4
 Identities = 13/53 (24%), Positives = 26/53 (49%)
 Frame = +3

Query: 183 SPCNVRPCVKMVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVKSMK 341
           +PC ++ C + +S+    V   ++K P   P    TL   ++G  +  +K +K
Sbjct: 398 NPCRIQYCTQEISMTPIHVLLLIVKAPILDPSLVVTLCSRFIGHQARKLKIVK 450



 Score = 23.8 bits (49), Expect(2) = 2.4
 Identities = 8/13 (61%), Positives = 9/13 (69%)
 Frame = +3

Query: 165 PGPQPPSPCNVRP 203
           PGPQ P P N+ P
Sbjct: 362 PGPQDPGPGNILP 374


>SB_14043| Best HMM Match : Extensin_1 (HMM E-Value=0.75)
          Length = 568

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 14/46 (30%), Positives = 22/46 (47%), Gaps = 1/46 (2%)
 Frame = +3

Query: 132 TSKTHTLRPETPGPQPPSPCNVRPCVKMVSLC*RVVH-ARLLKCPH 266
           T+K HT +P T  P    P N+ P +   +L   ++H     + PH
Sbjct: 168 TTKPHTTKPHTTKPHTTKPHNIDPTLPSPTLLNALLHFLYFYQAPH 213



 Score = 27.9 bits (59), Expect = 7.6
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +3

Query: 108 KFKTQQWVTSKTHTLRPETPGPQPPSPCNVRPC 206
           K KT +  T+K +T +P T  P+   P   +PC
Sbjct: 100 KPKTTKPHTNKPYTTKPRTTKPRTTKPHTTKPC 132


>SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1438

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 21/84 (25%), Positives = 37/84 (44%)
 Frame = +2

Query: 350 PSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGXE 529
           P +  + V   +  +++  D   +      ADN + RE+    + +L ++  T    G  
Sbjct: 284 PESDGIRVDETENGEHEAVDELPEDKPDTEADNYEQREETPTKEDELKSECTTSDSEGTP 343

Query: 530 LLCTVLKSCGEECVIAVKANTALD 601
              T  KS GEE V A ++  +LD
Sbjct: 344 SAATYGKSDGEENV-AQESEESLD 366


>SB_605| Best HMM Match : Calx-beta (HMM E-Value=0)
          Length = 1958

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 11/30 (36%), Positives = 20/30 (66%)
 Frame = -2

Query: 154 LKVCVFDVTHCCVLNLTTVRNDKIKKITIY 65
           + + +F VT+CC L LT +   + +++TIY
Sbjct: 14  VNLIIFTVTNCCFLVLTALIGFRDRQVTIY 43


>SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 154

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +2

Query: 209 KNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVGIDIFNG 328
           + G +M +G+PCKI   +   K G HG   +H+ G D  NG
Sbjct: 17  RRGVMMAEGKPCKITGTIEGLKAGNHGF-HIHVYG-DNTNG 55


>SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1671

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 16/43 (37%), Positives = 18/43 (41%)
 Frame = +2

Query: 323 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNG 451
           NG  Y   CP   N D    + EDY  +   D  YLT   D G
Sbjct: 400 NGHTYHMTCPGQTNFDPAKKRCEDYDCSG-RDVAYLTDQNDGG 441


>SB_1052| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1092

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 30/131 (22%), Positives = 53/131 (40%), Gaps = 8/131 (6%)
 Frame = +2

Query: 143  THFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKT-----GKHGHAKVHLV 307
            T  E+G   +    P QC+    + F+    RP + +  S ++      G     +  L 
Sbjct: 865  TRRESGQRVSHGLSPKQCAVSVVSSFMDSGARPRRFLPGSQARPRRFLPGSQPRPRRFLP 924

Query: 308  GIDIFNGKKYE--DICPSTHNMDVPHVKREDYQLTDISD-DGYLTLMADNGDLREDLKIP 478
             + +  G  Y   ++ P    +D+     + Y++ ++   D YL L  D GD    LK+ 
Sbjct: 925  VLKLDPGDFYRVLNLDPGDFYLDLKLDPGDFYRVLNLDPGDFYLDLKLDPGDFYPVLKLD 984

Query: 479  DGDLGTQLRTD 511
             GD    L+ D
Sbjct: 985  PGDFYPVLKRD 995


>SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13)
          Length = 492

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 17/44 (38%), Positives = 22/44 (50%)
 Frame = -3

Query: 558 PQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSSRRSPLSAIRVR 427
           P+  S V + +LP+   V  C+P SPS I RS    P     VR
Sbjct: 253 PRQISNVRSLTLPVRYQVIGCLP-SPSDIKRSVPYPPRQISNVR 295


>SB_43823| Best HMM Match : MAM (HMM E-Value=0)
          Length = 1724

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 2/28 (7%)
 Frame = -1

Query: 221  RNHFYAGPNIAW--GRWLRPRSLRSQSV 144
            R  F+  PN+A   G+WL P+SLR+  V
Sbjct: 947  REGFFNNPNLAGCKGQWLGPKSLRASRV 974


>SB_49607| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 4538

 Score = 25.0 bits (52), Expect(2) = 7.1
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +3

Query: 135  SKTHTLRPETPGPQPPSPCNVRPCV 209
            SK+H ++  T GP+  SP N R  +
Sbjct: 4412 SKSHPMQARTQGPRTHSPYNGRKSI 4436



 Score = 21.0 bits (42), Expect(2) = 7.1
 Identities = 11/29 (37%), Positives = 15/29 (51%)
 Frame = +3

Query: 255  KCPHPKPESTATLKFTWLGLISSMVKSMK 341
            K P   P    ++ FTW   ISS+  SM+
Sbjct: 4474 KNPGYGPAKELSIAFTWGFSISSLRSSMQ 4502


>SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 523

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
 Frame = +2

Query: 365 MDVPHVKREDYQLTDISDDGYLT----LMADNGDLREDLKIPDGDLGTQLRTDFDSGXEL 532
           +D+P+ K + + L  ++DD  L+    +  D   L E L  PD  +    R   D G E 
Sbjct: 37  LDIPYEKYDKFDLESLTDDECLSEFRFIKNDLYRLNEALNFPD-QITCPNRLTVD-GMEA 94

Query: 533 LCTVLKSCGEEC 568
           LC  L+     C
Sbjct: 95  LCMTLRRFAYPC 106


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,478,188
Number of Sequences: 59808
Number of extensions: 462416
Number of successful extensions: 1428
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1264
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1413
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1669334250
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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