BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= fe100P01_F_O18 (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 198 2e-51 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 192 2e-49 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 187 5e-48 At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.7 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.6 At5g64290.1 68418.m08076 oxoglutarate/malate translocator, putat... 28 4.7 At5g64280.1 68418.m08075 oxoglutarate/malate translocator, putat... 28 4.7 At3g51290.1 68416.m05614 proline-rich family protein 28 4.7 At3g49180.1 68416.m05375 transducin family protein / WD-40 repea... 28 6.2 At3g06490.1 68416.m00753 myb family transcription factor (MYB108... 28 6.2 At2g28550.1 68415.m03468 AP2 domain-containing transcription fac... 27 8.2 At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 27 8.2 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 27 8.2 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 198 bits (484), Expect = 2e-51 Identities = 86/150 (57%), Positives = 116/150 (77%), Gaps = 1/150 (0%) Frame = +2 Query: 137 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 316 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 317 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LG 493 IF KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLL 123 Query: 494 TQLRTDFDSGXELLCTVLKSCGEECVIAVK 583 Q+++ FD G +L+ +V+ + GEE + A+K Sbjct: 124 QQIKSGFDDGKDLVVSVMSAMGEEQINALK 153 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 192 bits (467), Expect = 2e-49 Identities = 84/150 (56%), Positives = 114/150 (76%), Gaps = 1/150 (0%) Frame = +2 Query: 137 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 316 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 317 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 493 IF KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L Sbjct: 64 IFTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALL 123 Query: 494 TQLRTDFDSGXELLCTVLKSCGEECVIAVK 583 TQL+ F+ G +++ +V+ + GEE + A+K Sbjct: 124 TQLKNGFEEGKDIVVSVMSAMGEEQMCALK 153 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 187 bits (456), Expect = 5e-48 Identities = 82/150 (54%), Positives = 110/150 (73%), Gaps = 1/150 (0%) Frame = +2 Query: 137 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 316 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 317 IFNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLG 493 IF KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Sbjct: 64 IFTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLT 123 Query: 494 TQLRTDFDSGXELLCTVLKSCGEECVIAVK 583 Q+R FD G +++ +V+ S GEE + AVK Sbjct: 124 AQMRLGFDEGKDIVVSVMSSMGEEQICAVK 153 >At5g14230.1 68418.m01663 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 591 Score = 29.1 bits (62), Expect = 2.7 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +2 Query: 467 LKIPDGDLGTQLRTDFDSGXELLCTVLKSCGEEC 568 L IPDGD T L G +C L SCG C Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -3 Query: 237 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 130 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At5g64290.1 68418.m08076 oxoglutarate/malate translocator, putative similar to SWISS-PROT:Q41364 2-oxoglutarate/malate translocator, chloroplast precursor. [Spinach]{Spinacia oleracea} Length = 563 Score = 28.3 bits (60), Expect = 4.7 Identities = 16/71 (22%), Positives = 28/71 (39%) Frame = +3 Query: 165 PGPQPPSPCNVRPCVKMVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVKSMKI 344 P P PSP K++ L + +L+ P PE + L + S + + + Sbjct: 80 PPPPSPSPSPSPQGAKLIPLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVL 139 Query: 345 SVPPHTTWTYL 377 S P W ++ Sbjct: 140 SPLPVGAWAFI 150 >At5g64280.1 68418.m08075 oxoglutarate/malate translocator, putative similar to SWISS-PROT:Q41364 2-oxoglutarate/malate translocator, chloroplast precursor [Spinach]{Spinacia oleracea} Length = 549 Score = 28.3 bits (60), Expect = 4.7 Identities = 15/75 (20%), Positives = 31/75 (41%) Frame = +3 Query: 153 RPETPGPQPPSPCNVRPCVKMVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVK 332 +P+ P P PP P K++ L V +++ P+PE + + L + + Sbjct: 66 KPQPPPPSPPQPQG----AKLIPLAISVSIGLIVRFLIPRPEQVTSQGWQLLSIFLFTIS 121 Query: 333 SMKISVPPHTTWTYL 377 + + P W ++ Sbjct: 122 GLVLGPLPVGAWAFI 136 >At3g51290.1 68416.m05614 proline-rich family protein Length = 602 Score = 28.3 bits (60), Expect = 4.7 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = +3 Query: 114 KTQQWVTSKTHTLRPETPGPQPPSP 188 +T W T+ T ++ P P P PP P Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115 >At3g49180.1 68416.m05375 transducin family protein / WD-40 repeat family protein contains 5 WD-40 repeats (PF00400); GTP-binding protein beta chain homolog, Nicotiana tabacum, PIR:T16970 Length = 438 Score = 27.9 bits (59), Expect = 6.2 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = -2 Query: 595 SCVCFDGDDALLTAGFQHGA 536 +C+ F GDD+LL +G Q G+ Sbjct: 124 TCLVFSGDDSLLVSGSQDGS 143 >At3g06490.1 68416.m00753 myb family transcription factor (MYB108) identical to transcription factor MYB108 GI:15375290 from [Arabidopsis thaliana] Length = 323 Score = 27.9 bits (59), Expect = 6.2 Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Frame = +3 Query: 240 HARLLKCPHPKPESTATLKFTWLGLISSMVKSMKIS--VPPHTTWTYLT*SAKTTN 401 HA+ LKC + T+K+ W+ + ++S S TT T T SA T++ Sbjct: 122 HAKQLKCDVNSQQFKDTMKYLWMPRLVERIQSASASSAAAATTTTTTTTGSAGTSS 177 >At2g28550.1 68415.m03468 AP2 domain-containing transcription factor RAP2.7 (RAP2.7) nearly identical to AP2 domain transcription factor RAP2.7 (GI:2281639) [Arabidopsis thaliana] Length = 449 Score = 27.5 bits (58), Expect = 8.2 Identities = 9/15 (60%), Positives = 12/15 (80%) Frame = +3 Query: 138 KTHTLRPETPGPQPP 182 +T +RP+ PGPQPP Sbjct: 402 QTSAVRPQPPGPQPP 416 >At1g56345.1 68414.m06477 pseudouridine synthase family protein low similarity to SP|P23851 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil hydrolyase) {Escherichia coli}; contains Pfam profile PF00849: RNA pseudouridylate synthase Length = 322 Score = 27.5 bits (58), Expect = 8.2 Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 1/69 (1%) Frame = -2 Query: 547 QHGAQQFXAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVRY 371 +HGA + AA++V L +RD E + V+V S + + + D++ ++V A R Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIV-AEGER- 233 Query: 370 VHVVCGGTD 344 + CGG D Sbjct: 234 -ELSCGGDD 241 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.5 bits (58), Expect = 8.2 Identities = 15/45 (33%), Positives = 18/45 (40%), Gaps = 3/45 (6%) Frame = +3 Query: 138 KTHTLRPETPGPQPPS---PCNVRPCVKMVSLC*RVVHARLLKCP 263 KT T P PP PCN PC + S + + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,933,353 Number of Sequences: 28952 Number of extensions: 308595 Number of successful extensions: 933 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 870 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 930 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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