Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_O17
(624 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenas... 150 8e-37
At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenas... 148 3e-36
At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenas... 144 3e-35
At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenas... 142 1e-34
At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenas... 109 1e-24
At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenas... 105 3e-23
At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenas... 103 1e-22
At1g21440.1 68414.m02681 mutase family protein similar to carbox... 28 5.8
At3g63330.1 68416.m07125 protein kinase family protein contains ... 27 7.7
>At1g79530.1 68414.m09271 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 422
Score = 150 bits (363), Expect = 8e-37
Identities = 68/98 (69%), Positives = 81/98 (82%)
Frame = +2
Query: 296 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPS 475
+L +NG K+ V S+RDP IPW GA+YVV S+GVFTT KA++HL+GGAKKVIISAPS
Sbjct: 150 TLEINGKKVNVVSKRDPSEIPWADLGADYVVESSGVFTTLSKAASHLKGGAKKVIISAPS 209
Query: 476 ADAPMFVVGVNLQXYDPSLKVISNASCTTNCLAPLAKV 589
ADAPMFVVGVN Y P++ ++SNASCTTNCLAPLAKV
Sbjct: 210 ADAPMFVVGVNEHTYQPNMDIVSNASCTTNCLAPLAKV 247
Score = 58.8 bits (136), Expect = 3e-09
Identities = 24/36 (66%), Positives = 28/36 (77%)
Frame = +1
Query: 184 QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 291
+VVA+NDPFI YM Y+ KYDSTHG FKGS+ V D
Sbjct: 112 EVVAVNDPFIDAKYMAYMLKYDSTHGNFKGSINVID 147
Score = 36.3 bits (80), Expect = 0.017
Identities = 15/17 (88%), Positives = 17/17 (100%)
Frame = +3
Query: 111 SKIGINGFGRIGRLVLR 161
+K+GINGFGRIGRLVLR
Sbjct: 87 TKVGINGFGRIGRLVLR 103
>At1g16300.1 68414.m01951 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative
similar to glyceraldehyde-3-phosphate dehydrogenase
[Pinus sylvestris] GI:1100223; contains Pfam profiles
PF02800: Glyceraldehyde 3-phosphate dehydrogenase
C-terminal domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 420
Score = 148 bits (358), Expect = 3e-36
Identities = 68/98 (69%), Positives = 82/98 (83%)
Frame = +2
Query: 296 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPS 475
+L +NG ++ V S+RDP IPW GAEYVV S+GVFTT +AS+HL+GGAKKVIISAPS
Sbjct: 148 TLEINGKQVKVVSKRDPAEIPWADLGAEYVVESSGVFTTVGQASSHLKGGAKKVIISAPS 207
Query: 476 ADAPMFVVGVNLQXYDPSLKVISNASCTTNCLAPLAKV 589
ADAPMFVVGVN + Y P++ ++SNASCTTNCLAPLAKV
Sbjct: 208 ADAPMFVVGVNEKTYLPNMDIVSNASCTTNCLAPLAKV 245
Score = 59.7 bits (138), Expect = 2e-09
Identities = 24/43 (55%), Positives = 31/43 (72%)
Frame = +1
Query: 163 ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 291
A+ +VVA+NDPFI YM Y+FKYDSTHG +KG++ V D
Sbjct: 103 ATFRDDIEVVAVNDPFIDAKYMAYMFKYDSTHGNYKGTINVID 145
Score = 36.3 bits (80), Expect = 0.017
Identities = 15/17 (88%), Positives = 17/17 (100%)
Frame = +3
Query: 111 SKIGINGFGRIGRLVLR 161
+K+GINGFGRIGRLVLR
Sbjct: 85 TKVGINGFGRIGRLVLR 101
>At1g13440.1 68414.m01570 glyceraldehyde 3-phosphate dehydrogenase,
cytosolic, putative / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase, putative very
strong similarity to SP|P25858 Glyceraldehyde
3-phosphate dehydrogenase, cytosolic (EC 1.2.1.12)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 338
Score = 144 bits (350), Expect = 3e-35
Identities = 69/105 (65%), Positives = 83/105 (79%), Gaps = 1/105 (0%)
Frame = +2
Query: 278 LRFRMDSLLVNGNK-IAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKK 454
L+ + D L+ G K + VF R+P+ IPWG+AGA++VV STGVFT DKA+AHL+GGAKK
Sbjct: 63 LKVKDDKTLLFGEKPVTVFGIRNPEDIPWGEAGADFVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 455 VIISAPSADAPMFVVGVNLQXYDPSLKVISNASCTTNCLAPLAKV 589
V+ISAPS DAPMFVVGVN Y L ++SNASCTTNCLAPLAKV
Sbjct: 123 VVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKV 167
Score = 49.2 bits (112), Expect = 2e-06
Identities = 18/29 (62%), Positives = 25/29 (86%)
Frame = +1
Query: 184 QVVAINDPFIGLDYMVYLFKYDSTHGRFK 270
++VA+NDPFI +YM Y+FKYDS HG++K
Sbjct: 31 ELVAVNDPFITTEYMTYMFKYDSVHGQWK 59
Score = 36.7 bits (81), Expect = 0.013
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = +3
Query: 96 LADNMSKIGINGFGRIGRLVLR 161
+AD +IGINGFGRIGRLV R
Sbjct: 1 MADKKIRIGINGFGRIGRLVAR 22
>At3g04120.1 68416.m00436 glyceraldehyde-3-phosphate dehydrogenase,
cytosolic (GAPC) / NAD-dependent
glyceraldehyde-3-phosphate dehydrogenase identical to
SP|P25858 Glyceraldehyde 3-phosphate dehydrogenase,
cytosolic (EC 1.2.1.12) {Arabidopsis thaliana}
Length = 338
Score = 142 bits (345), Expect = 1e-34
Identities = 68/105 (64%), Positives = 82/105 (78%), Gaps = 1/105 (0%)
Frame = +2
Query: 278 LRFRMDSLLVNGNK-IAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKK 454
L+ + + L+ G K + VF R+P+ IPW +AGA+YVV STGVFT DKA+AHL+GGAKK
Sbjct: 63 LKIKDEKTLLFGEKPVTVFGIRNPEDIPWAEAGADYVVESTGVFTDKDKAAAHLKGGAKK 122
Query: 455 VIISAPSADAPMFVVGVNLQXYDPSLKVISNASCTTNCLAPLAKV 589
V+ISAPS DAPMFVVGVN Y L ++SNASCTTNCLAPLAKV
Sbjct: 123 VVISAPSKDAPMFVVGVNEHEYKSDLDIVSNASCTTNCLAPLAKV 167
Score = 49.6 bits (113), Expect = 2e-06
Identities = 19/37 (51%), Positives = 30/37 (81%), Gaps = 1/37 (2%)
Frame = +1
Query: 184 QVVAINDPFIGLDYMVYLFKYDSTHGRFK-GSVEVQD 291
++VA+NDPFI +YM Y+FKYDS HG++K ++++D
Sbjct: 31 ELVAVNDPFITTEYMTYMFKYDSVHGQWKHNELKIKD 67
Score = 36.7 bits (81), Expect = 0.013
Identities = 16/22 (72%), Positives = 18/22 (81%)
Frame = +3
Query: 96 LADNMSKIGINGFGRIGRLVLR 161
+AD +IGINGFGRIGRLV R
Sbjct: 1 MADKKIRIGINGFGRIGRLVAR 22
>At1g12900.1 68414.m01498 glyceraldehyde 3-phosphate dehydrogenase,
chloroplast, putative / NADP-dependent
glyceraldehydephosphate dehydrogenase, putative similar
to SP|P25856 Glyceraldehyde 3-phosphate dehydrogenase A,
chloroplast precursor (EC 1.2.1.13) (NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A)
{Arabidopsis thaliana}; contains Pfam profiles PF02800:
Glyceraldehyde 3-phosphate dehydrogenase C-terminal
domain, PF00044: Glyceraldehyde 3-phosphate
dehydrogenase NAD binding domain
Length = 399
Score = 109 bits (263), Expect = 1e-24
Identities = 51/99 (51%), Positives = 66/99 (66%), Gaps = 1/99 (1%)
Frame = +2
Query: 296 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAP- 472
+L V+G I + S+R+P +PWG+ G + V+ TGVF D A HL+ GAKKV+I+AP
Sbjct: 129 ALSVDGKIIKIVSDRNPSNLPWGELGIDLVIEGTGVFVDRDGAGKHLQAGAKKVLITAPG 188
Query: 473 SADAPMFVVGVNLQXYDPSLKVISNASCTTNCLAPLAKV 589
D P +VVGVN + Y +ISNASCTTNCLAP KV
Sbjct: 189 KGDIPTYVVGVNAELYSHEDTIISNASCTTNCLAPFVKV 227
Score = 33.5 bits (73), Expect = 0.12
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = +3
Query: 114 KIGINGFGRIGRLVLRCFY 170
K+ INGFGRIGR LRC++
Sbjct: 66 KVAINGFGRIGRNFLRCWH 84
>At3g26650.1 68416.m03330 glyceraldehyde 3-phosphate dehydrogenase
A, chloroplast (GAPA) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit A
identical to SP|P25856 Glyceraldehyde 3-phosphate
dehydrogenase A, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit A) {Arabidopsis thaliana}
Length = 396
Score = 105 bits (251), Expect = 3e-23
Identities = 50/99 (50%), Positives = 63/99 (63%), Gaps = 1/99 (1%)
Frame = +2
Query: 296 SLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAP- 472
++ V+G I V S R+P +PW + G + V+ TGVF + A H+E GAKKVII+AP
Sbjct: 126 AISVDGKIIQVVSNRNPSLLPWKELGIDIVIEGTGVFVDREGAGKHIEAGAKKVIITAPG 185
Query: 473 SADAPMFVVGVNLQXYDPSLKVISNASCTTNCLAPLAKV 589
D P +VVGVN Y +ISNASCTTNCLAP KV
Sbjct: 186 KGDIPTYVVGVNADAYSHDEPIISNASCTTNCLAPFVKV 224
Score = 33.5 bits (73), Expect = 0.12
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = +3
Query: 114 KIGINGFGRIGRLVLRCFY 170
K+ INGFGRIGR LRC++
Sbjct: 63 KVAINGFGRIGRNFLRCWH 81
>At1g42970.1 68414.m04947 glyceraldehyde-3-phosphate dehydrogenase
B, chloroplast (GAPB) / NADP-dependent
glyceraldehydephosphate dehydrogenase subunit B
identical to SP|P25857 Glyceraldehyde 3-phosphate
dehydrogenase B, chloroplast precursor (EC 1.2.1.13)
(NADP-dependent glyceraldehydephosphate dehydrogenase
subunit B) {Arabidopsis thaliana}
Length = 447
Score = 103 bits (247), Expect = 1e-22
Identities = 51/102 (50%), Positives = 67/102 (65%), Gaps = 3/102 (2%)
Frame = +2
Query: 293 DSLLVNGNKIAVFSERDPKAIPWGKAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAP 472
+++ V+G I V S RDP +PW + G + V+ TGVF A H++ GA KVII+AP
Sbjct: 145 ETISVDGKLIKVVSNRDPLKLPWAELGIDIVIEGTGVFVDGPGAGKHIQAGASKVIITAP 204
Query: 473 S--ADAPMFVVGVNLQXYDPSL-KVISNASCTTNCLAPLAKV 589
+ AD P +V+GVN Q Y + +ISNASCTTNCLAP AKV
Sbjct: 205 AKGADIPTYVMGVNEQDYGHDVANIISNASCTTNCLAPFAKV 246
Score = 33.5 bits (73), Expect = 0.12
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = +3
Query: 114 KIGINGFGRIGRLVLRCFY 170
K+ INGFGRIGR LRC++
Sbjct: 83 KVAINGFGRIGRNFLRCWH 101
>At1g21440.1 68414.m02681 mutase family protein similar to
carboxyvinyl-carboxyphosphonate phosphorylmutase
GB:O49290 from [Arabidopsis thaliana]; similar to
carboxyphosphonoenolpyruvate mutase (GI:47149)
[Streptomyces hygroscopicus]; contains Prosite PS00161:
Isocitrate lyase signature
Length = 336
Score = 27.9 bits (59), Expect = 5.8
Identities = 13/52 (25%), Positives = 24/52 (46%)
Frame = +2
Query: 365 KAGAEYVVXSTGVFTTTDKASAHLEGGAKKVIISAPSADAPMFVVGVNLQXY 520
+ A + TG+ D+A+ ++E GA + AP D + +G + Y
Sbjct: 191 RTDARALSAKTGLSDAIDRANLYMEAGADASFVEAPRDDDELKEIGRRTKGY 242
>At3g63330.1 68416.m07125 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 376
Score = 27.5 bits (58), Expect = 7.7
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 399 VSLPLQIKHLLTWREVLKKLLYQLPVLMPPCLL 497
V+ L +H+ W E K+L Y+L LM C+L
Sbjct: 262 VTRALLDQHIRGWSENFKELAYKLRSLMEMCIL 294
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,520,656
Number of Sequences: 28952
Number of extensions: 281347
Number of successful extensions: 726
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 679
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 722
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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