Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fe100P01_F_O01
(504 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 28 0.92
SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 2.1
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 2.1
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 27 2.1
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 2.1
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 2.8
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 2.8
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 26 3.7
SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 4.9
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 4.9
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 4.9
SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch... 25 6.5
SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 6.5
SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 8.5
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 27.9 bits (59), Expect = 0.92
Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +2
Query: 305 LGDANGKAXEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESHK 475
+ +A KA ++LEQ+ + E L K H + E+ + +EKL A ++ + S +
Sbjct: 52 INEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEE 111
Query: 476 L 478
L
Sbjct: 112 L 112
>SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 762
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -3
Query: 376 LGRALDVLPRLFQSLXGLAVRVSERCPGDSW 284
+G + L RLF SL GL ++ PG+ W
Sbjct: 410 VGTVIQGLSRLFSSLYGLRFVPADISPGEVW 440
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 2.1
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 121 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 240
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 2.1
Identities = 15/36 (41%), Positives = 22/36 (61%), Gaps = 1/36 (2%)
Frame = +2
Query: 326 AXEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 430
A E +++ +IER+A K A + E+ ATALRE
Sbjct: 157 ASERAREAQSSIERSASLREKQAREEAERAATALRE 192
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 2.1
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 115 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 204
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 2.8
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +1
Query: 100 KAAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTKSKDA 219
K+ V R F + +EH+ K+LE+Q + L +SKDA
Sbjct: 195 KSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQSKDA 233
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 2.8
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +2
Query: 338 LEQSRQNIERTAEELRKAHPDVEK 409
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.8 bits (54), Expect = 3.7
Identities = 19/69 (27%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 293 SRAALGDANGKAXEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQE-S 469
+RAA +AN + E E ++ IE R+A ++ E+ + Q +E S
Sbjct: 798 ARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLERQRRIQQERDEEIS 857
Query: 470 HKLAXKVSS 496
KLA K ++
Sbjct: 858 RKLAEKAAA 866
>SPBC649.05 |cut12|stf1|spindle pole body protein Cut12
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 25.4 bits (53), Expect = 4.9
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +2
Query: 380 LRKAHPDVEKNATALREKLQAAVQNTVQE 466
L AH D+E T++RE+L++ + +E
Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 25.4 bits (53), Expect = 4.9
Identities = 18/68 (26%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +1
Query: 97 PKAAMVRRDAPDFFKDIEH--HTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQ 270
PKA R+ + DI+ T+E + +E + + + + KDG+++ +
Sbjct: 61 PKANRKRKQSYGLLHDIKELGGTEEDLELIEDVESDEELEFEEPNRKQPKKDGTDAFANE 120
Query: 271 LNAFAKSL 294
LN FAK L
Sbjct: 121 LNLFAKKL 128
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 25.4 bits (53), Expect = 4.9
Identities = 11/52 (21%), Positives = 25/52 (48%)
Frame = +2
Query: 323 KAXEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESHKL 478
KA + LE+ ++ E + EE+ H + T+ + + + +QE ++
Sbjct: 353 KACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEKSRV 404
>SPBC16E9.05 |erg6||delta-sterol C-methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.0 bits (52), Expect = 6.5
Identities = 16/47 (34%), Positives = 23/47 (48%), Gaps = 2/47 (4%)
Frame = -1
Query: 501 TFEDTXFANLWDSCTVFCTAACS--FSRRAVAFFSTSGWALRSSSAV 367
T + T N+WD TVF T+ +R +V F G A + +S V
Sbjct: 297 TGDITKCQNIWDVFTVFRTSRLGKLVTRYSVQFLEKIGVAAKGTSKV 343
>SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux
transporter Bfr1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1530
Score = 25.0 bits (52), Expect = 6.5
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -1
Query: 432 FSRRAVAFFSTSGWALRSSSAVRSMF 355
FSR V FFS AL+S S + +MF
Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595
>SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 432
Score = 24.6 bits (51), Expect = 8.5
Identities = 13/51 (25%), Positives = 26/51 (50%)
Frame = +2
Query: 344 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESHKLAXKVSS 496
++RQ E A+ +RK+ PDV + ++ +V + +E + +SS
Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.128 0.379
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,479,823
Number of Sequences: 5004
Number of extensions: 23952
Number of successful extensions: 120
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 119
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 200198394
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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